Method and system for realizing Y-STR typing on male individual by adopting 26 Y-STR genetic loci

A technology of Y-STR and gene loci, which is applied in the field of Y-STR typing and system of male individuals using 26 Y-STR loci, which can solve the problems of typing crosstalk and unreadable typing

Active Publication Date: 2017-05-31
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a method for Y-STR typing of male individuals using 26 Y-STR loci, by adopting specific Y-STR locus combinations and specific primers designed for these loci, so that in the typing process There will be no problems of unreadable typing, crosstalk, and misplacement of typing, and the genotypes of 26 Y-STR loci of male individuals can be accurately obtained, so as to realize family investigation and paternity identification of male individuals , the detection of male components in mixed male and female components, and the determination of multiple male mixed components to provide data support

Method used

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  • Method and system for realizing Y-STR typing on male individual by adopting 26 Y-STR genetic loci
  • Method and system for realizing Y-STR typing on male individual by adopting 26 Y-STR genetic loci
  • Method and system for realizing Y-STR typing on male individual by adopting 26 Y-STR genetic loci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1. Verification of the accuracy of the method and system for Y-STR typing of male individuals of the present invention

[0060] In this example, the male individual is 193 male human anticoagulant blood samples, whose individual source is known, but in the implementation of Example 1 of this application, its individual source is assumed to be unknown, and the method and system of this application are used to analyze It performs Y-STR typing, including:

[0061] 1) Using the DNA acquisition system in the system of the present invention to obtain the DNA of a male individual, 2) Using the composite detection system in the system of the present invention to obtain the genotypes of the 26 Y-STR loci of the DNA, and according to the genotype Obtain the Y-STR typing result of the individual, 3) use the deduction system described in the system of the present invention to carry out family investigation, paternity paternity identification, male and female The origin ...

Embodiment 2

[0090] The Y-STR typing system of this application is compatible with the existing Comparison of plus kits in pedigree judgment

[0091] Compared with the detection system of patent CN103866019, the Y-STR typing system of the present invention adds 5 Y-STR sites, which increases the ability to distinguish males from different families. In addition, the added Y-STR sites are all low-mutation Therefore, the 26 Y-STR sites in the system of the present invention are all low-to-medium mutation rate sites, which are more suitable for family screening, because high mutation sites are likely to lead to false exclusions in family screening.

[0092] Examples are as follows:

[0093] On July 30, 2016, Ma (female, 12 years old) was raped and killed in Changdong Village, Zhifang Town, Ruzhou City, Henan Province. Vaginal swabs and anal swabs were taken during the autopsy, and the pre-sperm spot test was positive , after DNA testing of the two specimens, the autosomal DNA and Y chromoso...

Embodiment 3

[0101] Example 3 Comparison between the detection system of the present invention and the detection system in the patent CN103866019

[0102] 1. In the detection system of the present invention and the patent CN103866019 detection system, the primers designed for each locus amplify the size range of the amplification products of each allele as follows:

[0103] Patent CN103866019:

[0104]

[0105] This application (which figure 1 The amplified product layout diagram of each locus of the application is shown):

[0106]

[0107] It can be seen from the black line that the amplification products of DYS460 and DYS389II in the patent CN103866019 are arranged too closely, which will cause the following two problems:

[0108] Question 1), the allelic typing standard (ladder) is incorrectly typed.

[0109] image 3 It shows the typing diagram of the allelic ladder (ladder) obtained by using the patent CN103866019 system. It can be seen that the shown patent CN103866019, th...

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Abstract

The invention provides a method and a system for realizing Y-STR typing on the male individual by adopting 26 Y-STR genetic loci. The method comprises the following steps: acquiring DNA of the male individual, and acquiring the genotypes of the 26 Y-STR genetic loci of the DNA, wherein the 26 Y-STR genetic loci are as follows: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS448, DYS456, DYS458, DYS635, GATA H4, DYS438, DYS439, DYS460, DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533; and acquiring the Y-STR typing result of the male individual according to the genotypes of the 26 genetic loci of the male individual. With the typing result, the operations including family checking, paternity identification, detection for the male element in male and female mixed samples, and the detection for the male element in a plurality of male mixed samples can be realized on the male individual.

Description

technical field [0001] The present invention relates to a typing method and system, in particular to a method and system for Y-STR typing of male individuals using 26 Y-STR loci. Background technique [0002] Tandem repeat sequence (short tandem repeat, STR) is a kind of polymorphic genetic marker system that widely exists in human chromosomal DNA. -7bp) and the length of the amplified products is less than 500bp, the number of the allele site represents the number of sequence repetitions. The allelic fragment lengths of STR loci are concentrated, so multiple STR loci can be amplified multiple times. Multiple STR loci are compounded and amplified, and the cumulative identification ability can approach or reach the level of DNA fingerprinting. [0003] The Y chromosome is a male-specific chromosome, and all male individuals of the same paternal line have the same Y-STR haplotype. As a supplementary method of autosomal testing, Y-STR test has important application value for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2525/151
Inventor 莫晓婷白雪张建孙辉姚伊人李万水赵兴春
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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