The invention belongs to the technical field of bio-
genetic engineering and in particular relates to a
genome editing method using an attachment carrier for encoding targeted
endonuclease and a kit. According to the
genome editing method, the attachment carrier is utilized to express the targeted
endonuclease, and the attachment carrier can realize nonconformable and
stable gene expression and simultaneously expresses a screening
gene; cells without getting plasmids can be removed by virtue of
drug screening; and when edition is finished, a
drug is removed, the cells can gradually lose the attachment carriers, and therefore, an editing
cell line without containing a foreign
gene is obtained. With the adoption of the
genome editing method, the editing time is prolonged, and the
gene editing efficiency is improved. The invention also provides the kit for editing a
specific chromosome sequence of the edited cells. The
genome editing method and the kit are suitable for genome edition of various eucaryotic cells.