6 results about "Specific chromosome" patented technology

The application discloses a method for detecting glioma chromosome abnormalities based on targeted sequencing, and belongs to the technical field of biological medicine. The method first acquires the allele frequency of a to-be-detected sample at preset SNP sites (covering 1p, 1q, 19p, 19q, chromosome 7 and chromosome 10), and then calculates and determines whether specific chromosome arms or chromosomes have loss of heterozygosity. Meanwhile, the copy number of the region where each SNP site is located is calculated based on the sequencing depth, and the total copy number of the above-mentioned chromosomes is obtained by integration. Finally, the loss of heterozygosity determination result and the chromosome copy number information are comprehensively combined, so that the simultaneous identification of 1p / 19q co-deletion, gain of chromosome 7 (+7) and deletion of chromosome 10 (-10) is realized. The method does not require paired samples, can accurately quantify the copy number, avoid false positives, and only needs to detect part of the SNP sites, that is, can be combined with hot spot mutation detection, thereby saving cost and improving detection efficiency.

A method includes steps of: obtaining an original section image that is related to a subject and that includes cell-image portions; for each of the cell-image portions, determining a number of specific protein signals as a specific protein number, determining a number of specific chromosome signals as a specific chromosome number, and calculating a ratio of the specific protein number to the specific chromosome number as an individual protein-to-chromosome ratio; selecting N number of critical cell-image portions from among the cell-image portions according to the individual protein-to-chromosome ratios; and determining a risk of the subject developing breast cancer based on the specific protein number and the specific chromosome number determined for each of the N number of critical cell-image portions.

The present application relates to the technical field of in-vitro diagnosis, and in particular to a cell detection kit for the abnormality of human chromosomes 3 and 10 and a use thereof. A probe set comprised in the kit uses a specific chromosome locus or a centromere as a target, or uses a combination of a plurality of specific chromosome loci or centromeres as a target, so that the kit can rapidly and effectively detect a variety of cancer samples comprising lung cancer, breast cancer, intestinal cancer, esophageal cancer, bladder cancer, liver cancer, gastric cancer, pancreatic cancer, ovarian cancer, cervical cancer or prostate cancer, and is suitable for cancer diagnosis and development and popularization of prognostic products.

The invention discloses a KASP molecular marker method related to powdery mildew resistance of highland barley and application of the KASP molecular marker method, and belongs to the technical field of molecular markers. Two groups of KASP molecular marker primers are developed by analyzing a highland barley genome and aiming at key SNP sites on a specific chromosome. Wherein the first group of primers is designed aiming at the SNP site of the 711384649 site on the second chromosome of the highland barley, and the second group of primers is designed aiming at the SNP site of the 711384825 site on the second chromosome of the highland barley. By utilizing the primer combinations, the resistance of highland barley plants to powdery mildew can be efficiently and accurately identified. The detection method disclosed by the invention has the advantages of high accuracy, rapid detection, low cost, short period, simplicity and convenience in operation and the like, can effectively assist in breeding work of a novel powdery mildew resistant highland barley variety, and has an important application value and a wide development prospect.

The present invention belongs to the technical field of molecular breeding, and specifically relates to an SNP locus combination associated with mastitis resistance traits in dairy cows, as well as probes, a chip, and applications thereof. The SNP locus combination comprises 348 SNP loci located at specific chromosomal positions, the physical positions of which are determined based on the ARS-UCD2.0 reference genome. The present invention further provides probes and a liquid-phase chip for detecting the SNP locus combination, which can be used for early selection and breeding of mastitis resistance in dairy cows, identification of germplasm resources, kinship analysis, and pedigree correction. By means of high-throughput genotyping technology, the present invention enables rapid and accurate evaluation of innate resistance in dairy cows, and has the advantages of high detection efficiency, low cost, and suitability for large-scale population screening, thereby having important significance for improving disease-resistant breeding efficiency in dairy cows and ensuring the safe production of dairy products.

The invention discloses a marker for prognosis layering of mucus fibrosarcoma and application of the marker, and belongs to the technical field of biological medicine. The MDM2 protein or the number 12 chromosome polysome has obvious correlation with the prognostic stratification of the mucus fibrosarcoma, the MDM2 protein or the number 12 chromosome polysome is used as the marker for the prognostic stratification of the mucus fibrosarcoma, and a reagent for preparing and detecting the two markers can be applied to judging the prognostic stratification of the mucus fibrosarcoma. The marker disclosed by the invention is abnormal in protein level or specific chromosome structure, and compared with a gene-level marker, the marker disclosed by the invention is easier to carry out standardized detection and interpretation through conventional technologies such as immunohistochemistry and the like, and is suitable for developing related prognosis detection reagents.