3 results about "Specific chromosome" patented technology

The application discloses a method for detecting glioma chromosome abnormalities based on targeted sequencing, and belongs to the technical field of biological medicine. The method first acquires the allele frequency of a to-be-detected sample at preset SNP sites (covering 1p, 1q, 19p, 19q, chromosome 7 and chromosome 10), and then calculates and determines whether specific chromosome arms or chromosomes have loss of heterozygosity. Meanwhile, the copy number of the region where each SNP site is located is calculated based on the sequencing depth, and the total copy number of the above-mentioned chromosomes is obtained by integration. Finally, the loss of heterozygosity determination result and the chromosome copy number information are comprehensively combined, so that the simultaneous identification of 1p / 19q co-deletion, gain of chromosome 7 (+7) and deletion of chromosome 10 (-10) is realized. The method does not require paired samples, can accurately quantify the copy number, avoid false positives, and only needs to detect part of the SNP sites, that is, can be combined with hot spot mutation detection, thereby saving cost and improving detection efficiency.

The present invention belongs to the technical field of molecular breeding, and specifically relates to an SNP locus combination associated with mastitis resistance traits in dairy cows, as well as probes, a chip, and applications thereof. The SNP locus combination comprises 348 SNP loci located at specific chromosomal positions, the physical positions of which are determined based on the ARS-UCD2.0 reference genome. The present invention further provides probes and a liquid-phase chip for detecting the SNP locus combination, which can be used for early selection and breeding of mastitis resistance in dairy cows, identification of germplasm resources, kinship analysis, and pedigree correction. By means of high-throughput genotyping technology, the present invention enables rapid and accurate evaluation of innate resistance in dairy cows, and has the advantages of high detection efficiency, low cost, and suitability for large-scale population screening, thereby having important significance for improving disease-resistant breeding efficiency in dairy cows and ensuring the safe production of dairy products.

The invention discloses a marker for prognosis layering of mucus fibrosarcoma and application of the marker, and belongs to the technical field of biological medicine. The MDM2 protein or the number 12 chromosome polysome has obvious correlation with the prognostic stratification of the mucus fibrosarcoma, the MDM2 protein or the number 12 chromosome polysome is used as the marker for the prognostic stratification of the mucus fibrosarcoma, and a reagent for preparing and detecting the two markers can be applied to judging the prognostic stratification of the mucus fibrosarcoma. The marker disclosed by the invention is abnormal in protein level or specific chromosome structure, and compared with a gene-level marker, the marker disclosed by the invention is easier to carry out standardized detection and interpretation through conventional technologies such as immunohistochemistry and the like, and is suitable for developing related prognosis detection reagents.