The invention provides a
CRISPR / Cas9 technology based preparation method and applications of a PI3K[gamma]
whole body knockout mouse and a kit. The preparation method comprises following steps: designing sgRNA for identify the
gene PI3K[gamma]; connecting the sgRNA to a
plasmid carrier, carrying out in-vitro transcription to obtain
RNA that can be applied to
microinjection; injecting the
RNA intoa mouse fertilized egg, after injection, giving birth to mice; identifying the
genotype of the mice, selecting mice, whose PI3K[gamma]
whole body knockout
genotype detection result is positive, and
mating picked mice with wild mice to obtain PI3K[gamma]
whole body knockout mice with a stable
genotype. Compared with a conventional
gene knockout technology, the operation is easier, the efficiency ishigher, homozygote
mutation is obtained more easily, and good foundation is provided for the research on functions of PI3K[gamma].