38results about How to "Stable integration" patented technology

The present invention provides an efficient gene delivery system using Adeno-Associated Viral (AAV) vector in gene therapy. Furthermore, the invention provides a combined AAV and Adenovirus (Adv) cocktail gene delivery system which is even more efficient in in vivo gene delivery and expression without eliciting any significant immune responses in an immunocompetent subject. In particular, the invention provides a therapeutic agent and methods for preventing, treating, managing, or ameliorating various diseases and disorders including, but not limited to, bone diseases, by delivering Bone Morphogenetic Protein 2 (BMP-2) for new bone formation via gene therapy using said system. The invention provides a nucleic acid molecule comprising an AVV vector and a promoter operably linked to a sequence encoding BMP-2; and a nucleic acid molecule comprising an Adv vector and a promoter operably linked to a sequence encoding BMP-2, as well as vectors and host cells comprising said nucleic acid molecules, respectively.

The present invention provides a method for producing a polyolefin-based resin crosslinked foamed sheet which is excellent in heat resistance and flexibility, has a small diameter of cells, can be developed in various utilities, and is excellent in vacuum moldability. The method for producing a polyolefin-based resin crosslinked foamed sheet according to the present invention comprises the steps of supplying a foamed sheet with closed cells, comprising a polyolefin-based resin, to a gap between one pair of rolls which have different circumferential speeds, and are rotated so that rotation directions on facing surfaces are the same direction, and applying a compression force and a shear stress to the foamed sheet with closed cells simultaneously to rupture a part of closed cells of the foamed sheet with closed cells to communicate closed cells into open cells.

The invention discloses a compound type piggyBac recombinant vector as well as a preparation method and an application of the compound type piggyBac recombinant vector. According to the recombinant vector, a target gene expression frame, selection marker gene expression frames I and II, piggyBac transposase expression frame regulated by a heat shock promoter and truncated type piggyBac transposon arms L2 and R2 are inserted between wild type piggyBac transposon arms R1 and L1, integration of target gene, selection marker gene, piggyBac transposase expression frame sequence in silkworm genome can be conveniently mediated, complete deletion of all of transposon sequences and selection marker gene sequences in progeny transgenosis silkworm genome can be realized through establishing and screening a transgenosis silkworm line with high target gene expression and then inducing piggyBac to express in the transgenosis silkworm by heat shock treatment, replacement of other exogenous gene and the target gene can be conveniently realized through box type exchange reaction mediated by phiC31 integrase by surplus target gene expression frame anchored by attP locus, and then fixed point integration of other exogenous genes in the genome locus is further realized.

The present invention provides a method of inhibiting angiogenesis within a tissue by providing exogenous PEDF to cells associated with the tissue. The presence of exogenous PEDF inhibits angiogenesis within the tissue, in part by interfering with the ability of vascular endothelia to expand within the tissue. The invention also provides a method for determining the severity of a tumor be assaying for the presence of PEDF within the tumor. To facilitate the inventive methods, the present invention provides pharmaceutical compositions including sources of PEDF.

The invention relates to an eyedrop bottle sorting and casing system device through a parallel four-axis robot provided with a visual system. The device comprises a working platform. A robot module, avisual system module and a workpiece platform module are arranged on the working platform. The robot module comprises the four-axis parallel robot and a control cabinet. The workpiece platform modulecomprises a photographing zone platform, a conveying belt, a charging zone and a discharging zone. The visual system module comprises a 2D camera, a light source, a computer and a visual software system. Specific to different gestures of eyedrop bottles in a detection zone, grabbing of different types is conducted, and a robot gas claw grabs the eyedrops and then sorts and cases the eyedrops. Inthe advanced manufacturing industry, the machine visual precision can be improved, the working efficiency can be improved, and the production cost is reduced.

This invention provides a method of conferring osmoprotection to plants. Plant plastid genomes, particularly the chloroplast genome, is transformed to express an osmoprotectant. The transgenic plants and their progeny display drought resistance. More importantly, such transgenic plants display no negative pleiotropic effects such as sterility or stunted growth.

The invention relates to a kitchen device (100) comprising: a grip (120) for manually holding the device, the grip defining a grip area (112), a first suction foot (130) attached to the device (100) and arranged to secure the device (100) to a surface (150) by 100 means of suction against the surface (150), an actuator (110) connected to the first suction foot (130) and extending in the grip area (112), the actuator (110) being arranged to transfer an actuating force (111) acting on the actuator (110) into a releasing force (131) acting on the first suction foot (130) to release the first suction foot (130) from the surface (150). This device can be released from the surface with an improved ease of use, because holding the device and releasing the device from the surface effectively have become a single, intuitive manual action by the user.

The present invention describes a modular hatrack (100) for an interior of an aircraft, wherein the modular hatrack (100) comprises at least one container (10) and at least one housing (20) to accommodate the container (10), wherein at least one component from the group comprising a personal supply channel (30), an electrical line (110), a light strip (11), an air duct (90) for an air conditioning system, and an air outlet (75) for an air conditioning system is integrated in the housing (20). The present invention also describes the use of the modular hatrack in an aircraft or in some other vehicle.

The invention provides a micron-nanometer structural bionic scaffold. Through the application of self-assembled collagen nano-fibers and bioactive glass nano-particles, strengthening can be performedon a scaffold respectively from the pores and the skeleton of a micron-sized silk fibroin porous scaffold, so that the micron-nanometer structural bionic scaffold strengthened on both inside and outside can be built. Through the discovery of experiments, the obtained bionic scaffold can have excellent mechanical properties, the collagen nano-fibers, the bioactive glass and silk fibroin can be stably integrated, and the osteogenesis of stem cells can be better promoted through the bionic structure of the bionic scaffold.

The invention discloses a construction method and application of expression vectors of a wild type hFIX (human coagulation factor IX) and three hFIX mutants in Pichia pastoris. The construction method comprises the following steps: according to a gene sequence provided by Genebank, cloning a hFIX full-length cDNA (complementary deoxyribonucleic acid) sequence from healthy human liver through a RT-PCR (reverse transcription-polymerase chain reaction) technology, constructing a yeast expression plasmid pPIC9K-hFIX which is used for Pichia pastoris expression and is fused with a yeast alpha-factor signal peptide and a full-length hFIX sequence, transferring the plasmid into Pichia pastoris SMD1168, and screening out a high-level expressing yeast strain; and then, constructing a yeast expression plasmid pPICAZ-hFIX, designing and constructing three yeast hFIX high-activity mutants on this basis, electrically transferring into Pichia pastoris SMD1168, and screening out high-level expressing yeast mutant strains. The coagulation activity of each of the obtained various hFIX yeast expression vectors is higher than that of a standard hFIX. The 50L pilot fermentation research on the wild type hFIX (pPIC9K-hFIX) shows that the maximum protein purification product secretion expression quantity can be up to 558 mg/L and the purity and coagulation activity are high.

Disclosed therein are a pulley assembly for a compressor and a manufacturing method of the same, which can prevent possible damages during processing and is fit for high-temperature environment because a pulley is made of magnesium alloy. The pulley assembly includes: a cylindrical hollow pulley made of magnesium alloy; an inner ring formed integrally with the pulley by insert injection molding, the inner ring being fixed to the inner circumferential surface of the pulley and made of a different material from the pulley; and a bearing forcedly pressed and fixed to the inner circumferential surface of the inner ring.

A recombinant marker gene encoding an orotate transporter polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO: 2, a polynucleotide construct comprising at least one copy of the recombinant marker gene, a cell comprising at least one exogenous copy of the marker gene, and a method of selecting or identifying a cell comprising at least one copy of the recombinant marker gene, and/or selecting or identifying a cell which has been cured of the recombinant marker gene.

The invention relates to construction and application of a novel angiotensin converting enzyme inhibitor screening model, and belongs to the technical field of biotechnology and food development. Specifically, the C structural domain protein of an angiotensin converting enzyme is successfully expressed by PCR, plasmid construction, protein extraction and the like, a stable liposome simulated membrane is obtained by combining repeated freezing and thawing with liquid nitrogen and extrusion with a liposome extruder, and finally the angiotensin converting enzyme bound with the simulated membraneis obtained; the enzyme activity of the system is measured by high-performance liquid chromatography, and it is verified that the model has practical significance. The constructed screening model canbe specifically used for exploring the functional rule of angiotensin converting enzyme inhibitors in membrane surface environments and is expected to provide a new idea for screening the angiotensinconverting enzyme inhibitors; meanwhile, the problem of large difference between in-vivo activity and in-vitro activity in screening of the angiotensin-converting enzyme inhibitors can be better solved.

The invention relates to universal carriers constructed based on methylotrophic hansenula polymorpha and pichia pastoris, in partituclar to a construction method of a universal carrier for performing heterologous protein expression on pichia pastoris and hansenula polymorpha. The construction method comprises the following steps that (1) a plasmid pHanMOX-GFP is digested through restriction enzymes Bgl II and BamH I, so that a pHan carrier is obtained; (2) a carrier plasmid pPinkAOX1-HPV16L1 is digested through restriction enzymes Bgl II and BamH I, a fragment PAOX1-HPV16L1-CYC1TT is recovered and then connected with the pHan carrier obtained in the step (1) through a T4 ligase at 16 DEG C overnight, an LB flat plate containing 0.1 mg/mL kanamycin is converted and coated, and plasmid enzyme digestion is extracted for identification, so that a restructured hansenula polymorpha plasmid pHanAOX1-HPV16L1 is obtained. The universal carrier is used for guiding expression of human papillomavirus type 16 major capsid protein L1 in pichia pastoris and hansenula polymorpha.

The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.

The invention relates to a method for specificity overexpression of miRNA of slow virus mediated mammal cells. The method aims at solving the problem that an existing miRNA expression system cannot achieve specificity expression of single miRNA of an miRNA complementary pair. By means of an shRNA system, an miRNA mature sequence is adopted as an upstream sense sequence, an antisense sequence complementing the miRNA mature sequence at the downstream is mutated according to T to C and A to G rules, the mutated RNA second-level neck ring structure is cloned to a carrier, then virus packaging is performed, and a cell line of the RNA second-level neck ring structure obtained after stable conversion mutation is built. One specific miRNA in the complementary miRNA pair can be overexpressed, and the method is applied to the field of miRNA overexpression.