5results about How to "Stable integration" patented technology

The present application belongs to the field of microbial metabolic engineering and industrial microorganism technology, and particularly relates to a method for stably integrating exogenous DNA into Pseudozyma roseolata, an iterative gene editing method and application thereof. The preservation number of the strain MYL-2 is CCTCC M 20252968, and the strain can efficiently accumulate oil under nitrogen-limited culture conditions, the main fatty acid components in the cells are C16-C18 fatty acids, and the strain has application potential as a microbial oil production strain. The present application provides a method for stably integrating exogenous DNA into Pseudozyma roseolata, and constructs a recombinant expression vector pNFH-loxp-RAD51 and a Cre recombination vector containing a loxP site, and for the first time, precise removal and repeated use of a screening marker mediated by a Cre-LoxP system are realized in the strain, thereby laying a foundation for multi-round gene editing and efficient engineering modification of the strain.

This invention relates to a novel method for producing cartilage tissue in vitro, as well as methods for therapeutic uses and screening of the cartilage tissue produced therefrom.

The invention relates to the technical field of power electronics, and discloses a wind-solar converter switching device optimization control method and system and a program product, and the method comprises the steps: obtaining a target multi-dimensional parameter set of a wind-solar converter, carrying out the power prediction through a long-short-term memory network model, and carrying out the dynamic parallel control of the wind-solar converter, and predicting device junction temperature distribution of the wind-solar converter in combination with a control result. Meanwhile, multi-parameter fusion diagnosis is carried out on fault states of parallel switching devices in the wind-solar converter, a fault redundancy switching strategy is determined based on the real-time fault states of the switching devices, and finally a parallel control result, a prediction result, a multi-parameter fusion diagnosis result and the fault redundancy switching strategy are integrated. According to the method, the number of normal parallel devices and the redundancy of a heat dissipation system are remarkably reduced on the premise that the current endurance capability and the thermal stability are ensured.

The invention provides cigarette paper with a temperature change indication function and a preparation method thereof, and relates to the field of cigarette paper. The cigarette paper with the temperature change indication function comprises a body paper base material and a temperature change functional coating, a micro-cavity array area is formed on the surface of one side of the body paper base material, the temperature change functional coating is coated on the micro-cavity array area on the surface of the body paper base material, and paint forming the temperature change functional coating comprises a thermochromic material and an adhesive. The micro-cavity array area is formed on the surface of one side of the body paper base material, and the temperature change functional coating is coated on the micro-cavity array area on the surface of the body paper base material, so that the temperature change functional coating is anchored in the micro-cavity, the temperature change functional coating is effectively and physically protected, stable integration of the temperature change functional coating is realized, and the temperature change functional coating is effectively prevented from being damaged. And meanwhile, a foundation is laid for realizing a precise regionalization indication function.

This invention relates to a genetically engineered strain for synthesizing retinal, its construction method, and its applications. The construction method involves introducing DNA fragments of the β-carotene 15,15'-oxygenase gene, isopentenyl pyrophosphate isomerase gene, phytoene dehydrogenase gene, geranyl-geranyl pyrophosphate synthase gene, and phytoene synthase gene into *Rhizopus cylindrica* to obtain a genetically engineered strain. This strain enhances the expression of β-carotene 15,15'-oxygenase, isopentenyl pyrophosphate isomerase, phytoene dehydrogenase, geranyl-geranyl pyrophosphate synthase, and phytoene synthase in *Rhizopus cylindrica*. By selecting *Rhizopus cylindrica* as a host, introducing key synthetic genes, and achieving stable genome integration, this method yields an engineered strain capable of efficiently synthesizing retinal using glucose as a carbon source, simplifying the production process, reducing costs, and improving industrial applicability.