117 results about "Oxygenase" patented technology

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses a heme oxygenase mutant and application of the heme oxygenase mutant in preparation of bilirubin through multienzyme coupling, and belongs to the field of biological catalysis engineering. The forward mutant of the heme oxygenase is obtained through a directed evolution technology, and heme can be efficiently catalyzed to synthesize biliverdin; the bilirubin is synthesized by further introducing biliverdin reductase, coupling cytochrome P450 reductase and formate dehydrogenase, and coupling the two core enzymes with foreign assistant enzymes to construct recombinant escherichia coli to catalyze heme to synthesize bilirubin. In the system, NADPH does not need to be exogenously added, the reaction time is about 4 h, the yield of bilirubin reaches 2.10 g / L, accumulation of intermediate products is not observed in the catalysis process, and the highest yield and the shortest reaction time in bilirubin synthesis reported at home and abroad at present are achieved. Compared with the traditional pig bile extraction, the method disclosed by the invention breaks through the resource limitation, is simple to operate and high in yield, and realizes efficient and green synthesis of bilirubin.

The invention discloses a cephalotaxus hainanensis Ch2OGD1 gene and application thereof, and belongs to the technical field of gene engineering. The Ch2OGD1 gene is used for coding a 2-oxoglutaric acid dependent dioxygenase (2OGD) type Pictet-Spengler enzyme, and the Pictet-Spengler enzyme is used for coding a Pictet-Spengler enzyme. Through a tobacco transient expression system and in-vitro enzyme activity determination, the Ch2OGD1 is proved to be capable of specifically catalyzing the condensation of dopamine and 4-hydroxyphenylpropanal to generate 1-phenethyl isoquinoline. The invention provides a directional preparation method of 1-phenethyl isoquinoline based on the Ch2OGD1 gene, provides a key enzyme element for biosynthesis of a cephalotaxine alkaloid precursor compound, and provides a technical basis for expanding the application of the gene in plant genetic engineering. And a potential technical path is provided for relieving the problem of resource shortage of cephalotaxine alkaloids.

A genetically modified yeast cell, wherein the yeast cell comprises a genetic modification comprising overexpression of yeast gene encoding porphobilinogen deaminase (HEM3), the HEM3 gene having at least 80% identity with SEQ ID No. 7. The genome of the modified yeast cell further comprises one or more genetic modifications in one or more genes selected from: genes coding for heme-dependent repressor of hypoxic genes (ROX1), genes coding for heme oxygenase (HMX1), genes coding for a receptor for vacuolar proteases (VPS10), and genes coding for vacuolar proteinase (PEP4), the one or more genetic modifications being such that expression of a polypeptide from such a gene is reduced or disrupted or the polypeptide expressed is non-functional.

1, 4-disubstituted-1, 2, 3 triazole compounds of formula (I): (I) capable of inhibiting heme oxygenase activity, their use as a medicament for the treatment and / or prophylaxis of pathological conditions associated with alteration of heme oxygenase activity and pharmaceutical compositions comprising the same.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

The application discloses a method for promoting transport and improving retinal synthesis, which is obtained by introducing an exogenous ABC transport protein Pdr5 into a host fungus and adding a copy of beta-carotene-15, 15'-monooxygenase BLH; wherein the host fungus is a genetically modified Pichia pastoris strain PP-BCCIV. The application further verifies the retinal production performance of the recombinant strain by screening different surfactants, and further improves the ability of Pichia pastoris to produce retinal. At the same time, all the retinal is extracted into the extracellular, and all the carotenes are left in the intracellular, so as to achieve the co-production of carotenes and retinal. The recombinant Pichia pastoris of the application has a simple construction method, can better promote the synthesis of retinal and other vitamin A, and can make the engineering strain utilize glucose and methanol to efficiently synthesize retinal through a 5 L fermentation tank, which is beneficial to industrial production.

The invention relates to the field of genetic engineering and fermentation engineering, and discloses a genetically engineered bacterium for producing melatonin and application of the genetically engineered bacterium in fermentation production of melatonin. According to the invention, genes of mtrA, PTPS, SPR, PCD and DHPR of related enzymes synthesized by coding 2-amino-6-(1, 2-dihydroxypropyl)-5, 6, 7, 8-tetrahydro-4 (1H)-pterin diketone and genes of L-2-amino-3 (beta-indole) propionic acid monooxygenase, oxymethyltransferase, N-acetyltransferase and decarboxylase are co-expressed on the genetically engineered bacterium, so that the gene engineering bacterium is obtained. The strain can be used for fermentation production of melatonin, and experiments show that the yield of the strain can reach 6480 mg / L.

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

Methods for producing organosulfur compounds from a prokaryotic cell comprising enzymes involved in the production of cysteamine, hypotaurine, and taurine. Said prokaryote may comprise a vanin selected from vanin-1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and / or a flavin-containing monooxygenase 1 (fmo1). Methods include the expression of organosulfur compounds in Corynebacterium glutamicum with incubation conditions to promote compound production.

The invention belongs to the technical field of biological catalysis, and discloses a non-specific peroxygenase mutant, a preparation method and application thereof, and a preparation method of calcifediol. Compared with a wild type non-specific peroxygenase (comprising an amino acid fragment with a sequence as shown in SEQ ID NO: 1), the non-specific peroxygenase mutant provided by the invention has one or more of Y47L mutation, L83F mutation and V112E mutation. The non-specific peroxygenase mutant has excellent enzyme activity, has the advantages of high conversion rate, mild reaction conditions, low cost and the like when being applied to preparation of calcifediol, and has a good industrial application prospect.

Disclosed herein is a molecule, or a pharmaceutically acceptable salt thereof, that has a formula M1DO1-L-ME3, M1DO1 is a moiety that binds to IDO1, L is a linker covalently attaching M1DO1 and ME3, and ME3 is a moiety that binds to an E3 ubiquitin ligase. Disclosed herein are also the uses of the molecule, or a pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same, in a method of treating cancer in a subject in need thereof.

The invention relates to gene modification, in particular to a method for improving the catalytic capacity of engineering P450 peroxygenase hydrogen peroxide and application of the method. The invention relates to a method for improving hydrogen peroxide catalytic capacity of P450 enzyme, which comprises the following steps of: identifying a channel of the P450 enzyme by using Caver Web 1.0 software by taking a heme cofactor of the P450 enzyme as a starting point; the method comprises the following steps: selecting a target channel with the channel length of 10-30, the curvature of 0-2 and the diameter of 0-3 as a target channel, then selecting amino acid of which the side chain faces the channel at an inlet or a bottleneck of the target channel as key amino acid, and then performing specific mutation on one or more key amino acids, further, at least one channel capable of improving the hydrogen peroxide catalytic capability of the P450 enzyme is obtained, so that the catalytic capability of the P450 enzyme is improved under the catalysis of hydrogen peroxide. The catalytic activity of a plurality of mutants obtained by the invention is compared with that of a wild enzyme, and the channel with high catalytic activity is an H2O2 channel. The application of the method can improve the hydrogen peroxide utilization capability of the P450 enzyme, and further improves the activity of the catalytic substrate of the P450 enzyme.

The present application provides a kind of cytochrome P450 monooxygenase gene and the protein coded by it, recombinant plasmid and application derived from ramie, the coding region sequence of the cytochrome P450 monooxygenase gene derived from ramie is as shown in SEQ ID NO:1, the amino acid sequence of the protein coded by it is as shown in SEQ ID NO:2.The present application takes ramie as research object, obtains ramie cytochrome P450 monooxygenase gene (BnMAX1) by three generations sequencing and gene annotation comparison.Longer coding sequence of the target gene is obtained by PacBio Sequel sequencing, which is simple, fast and easy to operate.The function of the gene is verified by cloning identification, bioinformatics, expression pattern and genetic transformation analysis, which shows that the gene has the effect of relieving plant cadmium toxicity.The acquisition and application of the gene provide basic theoretical support for molecular improvement of cadmium tolerance of ramie, and provide new technical means for how to improve the ecological restoration ability of ramie under heavy metal cadmium stress.

The present application belongs to the technical field of quorum quenching enzyme preparation, and particularly relates to a multi-target quorum quenching enzyme preparation and a preparation method and application thereof, such as being mainly used for inhibiting the virulence factors of Pseudomonas aeruginosa, and including acyl homoserine lactone acyltransferase AiiO protein and 3-hydroxy-4-oxoquinolone 2,4-dioxygenase AqdC protein, and the mass ratio of the two is 20:(1-25); such as being mainly used for inhibiting the virulence factors and the synthesis of biological membranes of Pseudomonas aeruginosa, and further including deferiprone, and the mass ratio of AiiO, AqdC and deferiprone is 20:(1-25):(0.695-2.78), the present application can target the Las, Rhl and PQS of Pseudomonas aeruginosa, the yield and stability of the prepared AqdC protein are both high, the two types of enzymes are used in combination after the regulation amount, and the virulence factors regulated by different quorum sensing pathways in Pseudomonas aeruginosa are effectively inhibited; the regulation amount of deferiprone is used in combination with the two types of quorum quenching enzymes, and the virulence and biological membranes of Pseudomonas aeruginosa can be effectively inhibited in a comprehensive manner.

The invention discloses a plant source leuco-anthocyanidin dioxygenase mutant and application thereof in synthesis of chiral drug intermediates, and belongs to the technical field of enzymology engineering. According to the invention, leuco-anthocyanidin dioxygenase (LDOX) derived from arabidopsis thaliana is modified through site-specific mutagenesis, an F304L / T239S double mutant (LDOXLS) is obtained, the (R)-phenylglycine analogue can be synthesized with the yield of 1.69 g / L, the yield of 81% and the enantiomeric excess ratio of 97: 3, and different substituent derivatives of the (R)-phenylglycine analogue can be generated. Compared with the traditional method, the method for synthesizing the (R)-phenylglycine analogue and the derivative thereof by using the enzyme has the advantages of wider substrate range, higher stereoselectivity, more environment-friendly requirement, simplicity and convenience in operation and the like.

The invention relates to the technical field of bioengineering bacteria. The invention provides a saccharomyces cerevisiae engineering bacterium capable of synthesizing p-anisaldehyde from the beginning and a construction method of the engineering bacterium. A saccharomyces cerevisiae recombinant strain JS1-15 is used as an original strain; the saccharomyces cerevisiae engineering bacteria are obtained by heterologous expression of 4-hydroxymandelic acid synthetase HmaS, heme oxygenase HMO, benzoyl formate decarboxylase BFD and methyltransferase 4VPMT1. The saccharomyces cerevisiae engineering bacteria are used for preparing the saccharomyces cerevisiae engineering bacteria. The saccharomyces cerevisiae engineering bacterium disclosed by the invention can realize a complete de novo biosynthesis path from 4-hydroxyphenylpyruvic acid to p-anisaldehyde by taking glucose as a unique carbon source, and has good industrial application potential.

The application discloses a cyclohexanone monooxygenase mutant and application thereof in epsilon-caprolactone synthesis, and belongs to the technical field of bioengineering. The application discloses a cyclohexanone monooxygenase mutant, a nucleic acid for coding the cyclohexanone monooxygenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, and a recombinant cyclohexanone monooxygenase mutant catalyst. Compared with other catalysts for preparing epsilon-caprolactone, the cyclohexanone monooxygenase mutant provided by the application has the advantages of high catalytic activity and good thermal stability, and shows strong application potential and wide application prospect in industrial application.

Methods and systems for cell-free bioproduction of retinal and retinol are provided, which may be performed using a combination of a beta-carotene 15,15'-dioxygenase and an alcohol dehydrogenase. Cell-free retinoid production enables reaction rates and productivity not possible / not obtained in cell-based systems.

The invention belongs to the technical field of enzyme engineering and biological catalysis, and particularly relates to a non-specific peroxygenase mutant and application thereof. The catalytic activity of the mutant protein is remarkably improved compared with that of parent protein, so that the production of 25-hydroxyvitamin D3 or 25-hydroxyvitamin D2 catalyzed by non-specific peroxygenase is promoted, and a novel enzyme for production and a synthetic route are provided.

The present application relates to the technical field of biosynthesis, and specifically discloses a method for selectively hydroxylating 16-position of dehydroabietyl acid based on CYP153 family cytochrome P450 enzyme, which comprises the following steps: plasmid construction, heterologous expression of CYP153A99-RhFRED in Escherichia coli, heterologous expression of opt13 in Escherichia coli, and crude enzyme catalytic conversion reaction. By optimizing the codon bias of the protein sequence of cytochrome monooxygenase in the CYP153 family, the p450 enzyme CYP153A99 with the biological catalytic function of catalyzing the hydroxylation of C16 position of dehydroabietyl acid is obtained, high-site selective oxidation of dehydroabietyl acid is realized, the substrate spectrum and application field of the CYP153 family P450 enzyme are expanded, and a new technical path is provided for the green synthesis of dehydroabietyl acid derivatives.

The present invention provides methods for treating a cancer in a subject and for inhibiting tumor growth, metastasis or a dihydrorotate oxygenase enzyme activity of a tumor or cancer cell. At least one trisubstituted benzotriazole derivative with the formula (I)is administered to the subject or is contacted with the cancer cell. Compounds of formula (I) have substituents R1, R2 and R3 which have the meanings given in the specification, and pharmaceutically acceptable salts thereof.

The invention discloses application of a premna microphylla turcz diene oxygenase gene NtPO, the nucleotide sequence of the premna microphylla turcz diene oxygenase gene NtPO is shown as SEQ ID NO.1, the premna microphylla turcz diene oxygenase gene NtPO contains 450 basic groups, the gene is derived from tobacco, and the premna microphylla turcz diene oxygenase gene NtPO is obtained by editing the NtPO gene. The topped plant height, stem girth, waist leaf length, waist leaf width and leaf area of the gene editing plant in the mature period are all higher than those of a control plant. In a word, according to the application, the NtPO gene is knocked out by utilizing a CRISPR / Cas9 mediated gene editing technology to obtain a gene editing plant which affects the growth and development of the plant, and a genetic material and a theoretical basis are provided for tobacco premna microphylla diene oxygenase gene research and tobacco growth and development research.

The present application provides a kind of herbicide composition for anti-benzoxyacetone phenoxy acid double oxygenase inhibitor and the herbicide safener containing quinoline structure;The herbicide composition prepared by the present application can reduce the phytotoxicity of herbicide to gramineous crops such as wheat, rice and millet without affecting the efficacy of herbicide;For three different gramineous crops such as wheat, rice and millet, the composition of the present application is significantly better than the composition for reducing the phytotoxicity of herbicide to rice and millet in reducing the phytotoxicity of herbicide to wheat.

The application discloses a construction method of a recombinant strain of Zymomonas mobilis for producing melanin and application of the recombinant strain. The recombinant strain is transformed with an expression plasmid containing a p-hydroxyphenylpyruvate dioxygenase hppD gene and a phenylalanine-4-hydroxylase pah gene. The nucleotide sequence of the p-hydroxyphenylpyruvate dioxygenase hppD gene is shown as SEQ ID NO:1, and the nucleotide sequence of the phenylalanine-4-hydroxylase pah gene is shown as SEQ ID NO:2. The application successfully realizes the heterologous synthesis of melanin in the Zymomonas mobilis by introducing the HGA synthesis pathway based on the analysis of the melanin production pathway. The introduction of the key metabolic pathway endows the Zymomonas mobilis with a brand-new metabolic capacity, and greatly expands the application boundary of the Zymomonas mobilis in the field of biosynthesis.

The application discloses a Talaromyces with multi-oxidase synergistic expression capacity and a fermentation method and application thereof. Talaromyces sp. The Talaromyces (Talaromyces autumnalis) Ta-lac is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC No. 42125. Compared with the engineering strain for biosynthesis of laccase, the strain has strong environmental adaptability, can naturally synthesize laccase, and has relatively stable genome, no potential risk of exogenous genes and relatively high safety. The Talaromyces has a short growth cycle, high yield of laccase, nitropropane dioxygenase, hydrogenase coenzyme dioxygenase and squalene monooxygenase and high lignin degradation activity in a fermentation process. The Talaromyces Ta-lac is applied to degradation of toxin dye wastewater, and has remarkable detoxification and decolorization effects.

The invention discloses a mutant containing flavin monooxygenase and application of the mutant. According to the invention, semi-rational design and screening are carried out on the flavin-containing monooxygenase to obtain the flavin-containing monooxygenase mutant, and the amino acid sequence of the flavin-containing monooxygenase mutant is shown as SEQ ID NO.4. The mutant provided by the invention can obviously improve the activity of the flavin-containing monooxygenase and is used for improving the yield of the teer violet. By using the engineering bacteria expressing the flavin-containing monooxygenase mutant, the activity of the flavin-containing monooxygenase can be remarkably improved, the engineering bacteria are used for producing the tylosin-containing monooxygenase mutant to improve the yield, the yield of the tylosin-containing monooxygenase mutant is improved to 74.6%, and the titer of the tylosin-containing monooxygenase mutant can reach 853.7 mg L <-1 >.

The invention provides a method for producing beta-hydroxy-beta-methylbutyric acid through double-enzyme co-expression, and relates to the technical field of enzyme engineering. According to the invention, L-amino acid deaminase and 4-hydroxyphenylpyruvate dioxygenase are expressed by using the same promoter in a host cell, and a key RBS combination is determined by optimizing and screening ribosome binding site RBS sequences, so that expression balance and efficient synergy of the two enzymes are realized; on the basis, biosynthesis of beta-hydroxy-beta-methylbutyric acid is carried out by taking L-leucine as a substrate, the yield of beta-hydroxy-beta-methylbutyric acid reaches 14.12 mM, and the molar conversion rate is 56.5%. Through series co-expression design of double enzymes, efficient biosynthesis of beta-hydroxy-beta-methylbutyric acid is achieved, and the method has the advantages of being mild in reaction condition, high in product purity, environmentally friendly and the like and is suitable for industrial popularization.