203 results about "Monoamine oxidase" patented technology

The invention belongs to the field of medicine, and in particular relates to a medical application of 2-([1,1'-biphenyl]-4-yl)-2-oxoethyl 4-((3-chloro-4-methylphenyl) amino)-4-oxobutanoate in a selective histone lysine-specific demethylase 1 (LSD1) inhibitor, especially the application in anti-tumor medicaments. Pharmacodynamic tests indicate that the 2-([1,1'-biphenyl]-4-yl)-2-oxoethyl 4-((3-chloro-4-methylphenyl) amino)-4-oxobutanoate has a remarkable LSD1 inhibiting effect, and has selectivity to homologous proteins MAO-A (monoamine oxidase-A) and MAO-B.

The invention belongs to the technical field of environmentally water treatment and particularly relates to a water purifier for treating production wastewater of a rubber accelerator NOBS and a wastewater treatment method. The water purifier comprises a flocculating water purifier, a microbial water purifier and an enzyme preparation, wherein the flocculating water purifier consists of polyaluminum ferric chloride, polyacrylamide, ferrous sulfate and aluminum sulfate; the microbial water purifier consists of nitrobacteria powder, paracoccus denitrificans powder, thiobacteria powder, aniline degradation bacteria powder, flocculating bacteria powder, bacillus siliceous powder and bacillus subtilis powder; the enzyme preparation consists of pectinase, cellulose, oxidordeuctase, xylanase, alkaline protease and monoamine oxidase. The water purifier for treating sewage provided by the invention is good in water purification effect, quick in water purification speed, high in safety and free of secondary pollution. By using a material with a desorption capacity as a raw material, sewage is treated by being assisted by microorganisms, so that the water purifier is wide in application range, and the treated water is extremely high in transparency and can be recycled.

The invention discloses lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.6430. Raw milk is used as a growth medium of the strain, and the monoamine oxidase inhibition rate of the obtained fermented milk can reach 52.4-78.8%. The lactobacillus rhamnosus can be used as a functional additive for producing beverages and dietary supplements with the functions of inhibiting the monoamine oxidase activity, fighting Parkinson's disease, easing depression or defying aging, and can also be applied to preparation of drugs for fighting Parkinson's disease or easing depression.

The invention provides novel compounds of formula I: that are monoamine oxidase-B inhibitors, which can be useful in treating obesity, diabetes, and / or cardiometabolic disorders (e.g., hypertension, dyslipidemias, high blood pressure, and insulin resistance).

The invention discloses pleurotus citrinopileatus sing ultrafine power cultured by using ramulus mori stems as major ingredients and a production method of the pleurotus citrinopileatus sing ultrafine power. The production method is characterized in that the ramulus mori stems is adopted as the major ingredients of the pleurotus citrinopileatus sing ultrafine power, 35-55 DEG C low-temperature vacuum drying is carried out for 4 to 5 hours, the crushing is carried out by a 500-1000-mesh sieve ultrafine crushing machine, and the pleurotus citrinopileatus sing ultrafine dry power is obtained. The pleurotus citrinopileatus sing ultrafine power and the production method have the advantages that the original nutrition and health care characteristics of pleurotus citrinopileatus sing can be maintained, the activity of B type monoamine oxidase is inhibited, in addition, the peroxyl lipid content of the liver can be reduced, the lipofuscin content of the heart is reduced, the activity of SOD (super oxygen dehydrogenises) in red blood cells can be improved, good effects of nourishing yin and yang, delaying senescence, preventing and resisting cancer, lowering the blood pressure and the like are realized, in addition, more convenient edible performance and efficient utilization performance of the health care ingredients are realized, and meanwhile, the silkworm industrial chain can be favorably prolonged.

The present invention provides a method for producing an alkaloid, for example, reticuline, comprising providing dopamine as a substrate for a series of actions of monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase.

The invention discloses a making method of chickpea natto. The method comprises the following steps of: soaking the chickpea for sprouting, steaming and boiling, inoculating, fermenting, after-ripening, freeze-drying and the like. In the method, the chickpea biochanin isoflavone is generated by carrying out the sprouting pretreatment on the chickpea; the nutritional ingredients of the chickpea are enhanced; and subsequently the natto bacillus subtilis is inoculated for fermentation so as to make the chickpea natto which is rich in chickpea flavonoid, bacillus natto and nattokinase. In addition, the invention also discloses the chickpea natto made by using the method and the application of the chickpea natto for preparing health-care foods or medicines for preventing or improving hyperlipidemia and resisting thrombosis. The experiments show that the chickpea natto disclosed by the invention can reduce the contents of the TC (total cholesterol), the TG (total glycerin) and the MAD (monoamine oxidase) of the organism blood serum, has the significant blood-fat depression and thrombolytic functions, and can be used for preparing the health-care foods or medicines for preventing or improving hyperlipidemia and resisting thrombosis.

The invention relates to an olefinic polymerization non-metallocene metal catalyst system and preparation and application thereof. A main catalyst is shown as a structural formula (1), wherein M represents Ti, Zr, Hf, Sc, Y, La, Nd or Sm; methylaluminoxane or modified methylaluminoxane is taken as a cocatalyst; and the molar ratio of the main catalyst to the cocatalyst is 1:100-5,000. The catalyst has high activity and high polymer molecular weight; when the catalyst is applied to copolymerization of ethylene and alpha-olefin or a polar alkene monomer, the insertion rate of the alpha-olefin or the polar alkene monomer is high; and the main catalyst has high stability and small monoamine oxidase (MAO) using amount.

The invention discloses a monoamine oxidase from Pseudomonas sp., a coding gene, a recombinant expression vector, a recombination expression transformant, and the synthesis application of the monoamine oxidase in tetrahydroquinoline chiral amine compounds. The coding gene of the monoamine oxidase MAO5-ZMU is from Pseudomonas sp. ZMU-T01. The amino acid sequence of monoamine oxidase protein is shown in SEQID No.1, and the corresponding coding gen nucleotide sequence is shown in SEQIDNo.2; the monoamine oxidase gene can be connected with an expression carrier to construct to obtain a recombination expression plasmid; the recombination expression plasmid is converted to escherichia coli to obtain genetically engineered bacteria with monoamine oxidase activity. The genetically engineered bacteria with monoamine oxidase activity can be used for effectively catalyzing 2-methyl-1,2,3,4-tetrahydroquinoline compounds for oxidation splitting, has the characteristics of high selectivity, moderatereaction condition, environment protection and the like and can be used for the biosynthesis of the chiral tetrahydroquinoline compounds.

The invention relates to a fluorescent testing method of monoamine oxidase activity. It forms fluorescent material, detects fluorescent intensity and achieves monoamine oxidase activity data by using monoamine oxidase to hydrolyze fluorescent probe. The probe is coumarin compound and its structure as shown in (I), in which R1 and R2 are alkyl of H or C1-C6 independently. Preparation of probe in this invention just needs simple device, convenient operation and easily gained materials. The prepared probe has stable property and high purity (above 99%). Compared to the current method for testing monoamine oxidase activity, its accuracy, sensitivity and testing velocity are all improved.

The invention provides novel compounds of formula I:that are monoamine oxidase-B inhibitors, which can be useful in treating obesity, diabetes, and / or cardiometabolic disorders (e.g., hypertension, dyslipidemias, high blood pressure, and insulin resistance).

The present invention provides a method for producing an alkaloid, for example, reticuline, comprising providing dopamine as a substrate for a series of actions of monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase.

The invention provides novel compounds of formulae I and II: that are monoamine oxidase-B inhibitors, which can be useful in treating obesity, diabetes, and / or cardiometabolic disorders (e.g., hypertension, dyslipidemias, high blood pressure, and insulin resistance).

The invention relates to a tacrine-coumarin derivative containing triazole and an application of the derivative and belongs to the technical field of biological medicine. The derivative comprises compounds represented as a formula I and pharmaceutically acceptable salt, and three preparation methods of the derivative are provided; a pharmacological experiment proves that the derivative is acetylcholinesterase inhibitor and can inhibit activity of acetylcholin esterase, butyrylcholine esterase and MAO (monoamine oxidase), and meanwhile, the derivative can inhibit autopolymerization of beta-amyloid protein, delay hydrolysis of acetyl choline and autopolymerization of beta-amyloid protein, improve the function of acetyl choline on synapsis and be used for treating Alzheimer's disease effectively.

The invention discloses a leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe as well as a synthetic method and biological application thereof. The adopted syntheticmethod includes the following step: (1) synthesizing the novel leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe (NML); and the biological application comprises the following step: (1) applying the probe to biological imaging and serum detection. The leucine aminopeptidase and monoamine oxidase-responsive fluorescent probe is synthesized for the first time, and the problem of low specificity of a traditional single-enzyme fluorescent probe is overcome; the fluorescent probe has near-infrared fluorescence properties, the background fluorescence signal can be effectively reduced, and the sensitivity of the probe can be improved; and the fluorescent probe has a good staining effect on living cells and high staining efficiency, and can detect cell endogenous leucine aminopeptidase and monoamine oxidase.

A method for treatment of neurodegenerative disease conditions stemming from multiple sclerosis, aging, autoimmune diseases and fibromyalgia. A compound effective to increase neuronal metabolism of histamine to a histamine H2 agonist is administered in an amount sufficient to stimulate production of cyclic AMP at a level which is sufficient to maintain myelin against undergoing self-degeneration. The compound is selected from the group consisting of histamine M-methyltransferase, monoamineoxidase-A, monoamineoxidase-A agonists and histamine H3 antagonists. The histamine M-methyltransferase may be administered to increase neuronal metabolism of histamine to tele-methylhistamine, whereas the monoamineoxidase-A or monoamineoxidase-A agonist may be administered so as to increase neuronal metabolism of telemethylhistamine to an H2 agonist. Separately or in conjunction with the others, the histamine H3 antagonist may be administered so as to inhibit metabolism of the telemethylhistamine to an H3 agonist, thereby increasing metabolism of the telemethylhistamine to an H2 agonist. The increased histamine H2 agonist levels reduce demyelination and the symptoms that are associated with these conditions.

The invention relates to a functional food for improving senile dementia in the field of functional foods. The functional food is prepared from the following raw materials: 10 to 50 parts of deep sea fish oil, 5 to 80 parts of grape pip, 10 to 70 parts of ginkgo leaf and 5 to 40 parts of anemarrhena. The deep sea fish oil is heated to the temperature of between 35 and 45 DEG C, and is added with dry extract powder prepared by extracting defatted grape pip, ginkgo leaf and anemarrhena, and the mixture is stirred, ground uniformly and filled. The functional food prepared from a few raw materials by a simple process can improve the content of catalase (CAT) in brain, reduce the activity of monoamine oxidase (MAO), antagonize oxidation reaction and prevent the reduction of monoamine neurotransmitter so as to achieve the effect of preventing and treating the senile dementia; in the aspect of preventing and treating alzheimer disease (AD), the functional food has the advantages of conditioning integrally and acting at multiple target spots; and after the functional food is prepared into capsules, the capsules are convenient to eat and store, long in quality guarantee period, attractive in appearance, easy to produce and high in bioavailability, and better realize the health-care function to benefit consumers.

The invention discloses a kit of single reagent used for diagnosing monoamine oxidase (MAO) in serum. MAO hydrolyzed benzylamine, butyl amine, amyl amine, Beta-phenylethyl amine and other amine compounds are utilized to produce oxygen; the oxygen reacts with glutamate dehydrogenase in a coupling way; oxidized coenzyme is formed by the reaction of reduced coenzyme; and the activity of sample MAO formed by quantitative reaction is calculated through measuring the descending speed of the absorbance of light with a wavelength of 340 nm; in addition, enzyme and substrate enzyme which can slowly generate reduced nicotinoyl coenzyme are added in the reaction process, so that an enzyme-substrate enzyme-nicotinoyl coenzyme slow reaction system is formed in single reagent and the nicotinoyl coenzyme can be compensated slowly and circularly and further the single reagent can be stabilized when the concentration of the single reagent achieves a certain balance. The kit is convenient for use and simple is structure, can be used on a common ultraviolet / visible light analyzer or a semi-automatic / full-automatic biochemical analyzer for rapid measurement without using a special or additional apparatus and has low cost.

The invention provides new oxazolidinones of formula (I), where R1, R2, R3 and R4 are independently selected from hydrogen, F and Cl; A is selected from certain heterocycles optionally substituted; X is selected from O, S, NRs and CR8Rg; Y is selected from O, S, SO, SO2, NO, NR11 and CR11R12; and n is selected from 0 and 1. The invention also provides different processes for the preparation of such compounds. Oxazolidinone compounds of formula (I) are active against Gram-positive and some Gram-negative human and veterinary pathogens with a weak monoamine oxidase (MAO) inhibitory activity. They are useful for the treatment of bacterial infections.

The invention discloses a process for producing 20 percent amitraz emulsifiable concentrate, which relates to the field of production and preparation of pesticides. A formulation of the amitraz emulsifiable concentrate comprises the following components: 202 kilograms per ton of raw amitraz, 120 kilograms per ton of emulsifying agent, 100 kilograms per ton of DMF, and 10 kilograms per ton of potentiating agent; and proper amount of dimethyl benzene is complemented. The amitraz emulsifiable concentrate is amitraz series broad spectrum acaricide, is mainly used for inhibiting the activity of monoamine oxidase, has the functions of contact toxicity, feeding resistance and repelling, and also has certain functions of internal absorption and fumigating. The amitraz emulsifiable concentrate is suitable for mites of various crops, and has better prevention effect on homoptera pests. The amitraz emulsifiable concentrate has a good pesticidal effect, avoids uninterrupted alternated use of pesticides, does not pollute the environment, and improves the quality and sanitation quality safety level of fruits.

The invention provides a fleece-flower root life-prolonging tablet for treating baldness according to the theory that the baldness is treated in need of treating liver and kidney firstly. The formula of the fleece-flower root life-prolonging tablet is as follows according to the weight ratio: 300-400 parts of polygonum multiflorum, 18-22 parts of rehmannia, 14-20 parts of semen sesami nigrum, 30-50 parts of the root of bidentate achyranthes, 150-200 parts of mulberry, 30-50 parts of fructus ligustri lucidi, 200-250 parts of eclipta, 30-50 parts of folium mori, 70-90 parts of semen cuscutae, 230-280 parts of fructus rosae laevigatae, 30-50 parts of fructus psoraleae, 60-100 parts of phlomis bracteosa royle and 15-25 parts of honeysuckle. The modern pharmacology research shows that the fleece-flower root life-prolonging tablet has the functions of resisting free radicals, preventing lipid peroxidation, reducing activity of monoamine oxidase, enhancing the function of adrenal gland and the like; in addition, the fleece-flower root life-prolonging tablet can lessen the formation of experimental atheromatous plaques and lipid deposition, so as to exert the functions of reducing blood fat and resisting atherosclerosis.

The invention discloses 2,3-dihydrobenzofuran derivatives shown in a formula (I), intermediates for preparing the derivatives and a preparation method of the derivatives. In the formula (I), R1 is shown in formulae (II) and (III), wherein R5 and R6 are independently substituted phenyl; R2 and R4 are independently benzoyl or substituted benzoyl; and R3 is H, hydroxy, halogen, nitro or C1-C3 alkyl. In the invention, novel 2-phenylseleno-methyl-2,3-dihydrobenzofuran compounds containing organic selenium and five-membered nitrogen-containing heterocycles are provided; the synthesis process is simple, is high in yield and is easy to industrialize; and the compounds are depressant medicaments with significant inhibition on monoamine oxidase activity, thereby providing a research basis for screening new medicaments such as antidepressant medicaments, anti-Parkinson medicaments and the like.

The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six indexes of diagnosis, forecast and prognosis of hepatitis, hepatocirrhosis and liver cancer, including hepatitis B virus surface antigen (HBsAg), hepatitis C virus antibody (HCVAb), alpha-fetoprotein (AFP), alpha-L-Fucosidase (AFU), mono amine oxidase (MAO), hyaluronic acid (HA). And the orifice plate comprises a substrate and reaction orifices on the substrate, where the reaction orifices comprise 2-384 sample orifices and 2-8 standard product orifices, and at the bottom of each reaction orifice is solid carrier coated with micro lattice of HBsAg, HCVAg, AFP, AFU,MAO,and HA antigens / antibodies or more. And the reaction plate and reagent can simply and conveniently, quickly and accurately implement simultaneous detection of the six indexes of diagnosis, forecast and prognosis of hepatitis, hepatocirrhosis and liver cancer for many persons.

The invention relates to the field of medicinal chemistry, in particular to a 4-hydroxycoumarin-3-Schiff base compound (I). Pharmacodynamic experiments prove that the compound is a multifunctional monoamine oxidase (MAO) inhibitor, has better effects of inhibiting beta-amyloid protein (A beta) aggregation, metal ion chelation, oxidation resistance and neuroprotection, and can be used for curing Alzheimer's disease.

The present invention provides methods of using Monoamine Oxidase C (MAO-C), also known as renalase, as a therapeutic protein in its active and inactive forms. Administering inactive renalase protein to individuals with lower renalase levels can be used to provide them with an adequate pool of the protein that can be activated by the body as needed. Active renalase protein can be administered to individuals needing an immediate reduction of catecholamine levels. An inhibitor of renalase may be used to enhance adrenergic action.

The invention discloses a compound shown as a formula (i1) or a formula (i2) and a preparing method thereof. The compound can be adopted as a fluorescence probe and applied for monoamines oxidase activity fluorescence detection. The compound has characteristics of simple preparation equipment, simple operation processes, commercialized and easily available raw materials, stable properties of the compound as the fluorescence probe for the monoamines oxidase, and high purity. When the fluorescence probe is applied for the monoamines oxidase activity fluorescence detection method, the accuracy and the sensitivity are largely improved than those of basic methods used in the prior art for detection the monoamines oxidase activity.

The invention relates to the application of a calcium disodium edentate and corticosteroid compound preparation in preparing medicine capable of inhibiting connective tissue growth factors and monoamine oxidase, and provides a compound preparation prepared from calcium disodium edentate and corticosteroid, which comprises the calcium disodium edentate which serves as the active component, the corticosteroid and one or more medicinal auxiliary ingredients; and the compound preparation contains the calcium disodium edentate in the weight percentage of 2 to 20 percent, glucocorticoid in the weight percentage of 0.01 to 0.1 percent, and the auxiliary ingredients which can be used for injection.

The invention discloses a composition for dredging breast microcirculation to maintain autologous glandular cells and a preparation method thereof. The composition takes oligopeptide-1 and multiple medicinal plant extracts as main components, and can balance the ratio of in vivo estradiol to progesterone mainly by reducing hormones released by autologous gonadotropin; moreover, the composition can promote metabolism of human breast tissues, inhibit the activity of monoamine oxidase in breast tissues and synthesis of collagenous fibers, and inhibit abnormal hyperplasia of endothelial growth factors and basic fibroblast growth factors of breast blood vessels. The composition can effectively dredge breast channels and collaterals, improve breast microcirculation, discharge toxins accumulated in breasts, eliminate breast catheter hydrops and hyperemia edema, and restore the health and vitality of breasts. The composition has the effects of dredging, discharging, repairing, nourishing and strengthening mammary glands to achieve breast strengthening, beautification and protection, has the effects of preventing mammary diseases and breast healthcare, can realize 'preventive treatment of diseases', and can improve the sub-health state of breasts while preventing the diseases.

The invention provides recombinant broad-spectrum metarhizium; the recombinant broad-spectrum metarhizium down-regulates the expression of monoamine oxidase or does not express the monoamine oxidase,or the content of tryptamine in a recombinant broad-spectrum metarhizium body is higher than that of tryptamine in wild type broad-spectrum metarhizium; the recombinant broad-spectrum metarhizium is the strain per se, the progenies of the strain, the conidia produced by the strain, the mycelia produced by the strain, or any combination of the progenies, the conidia and the mycelia. A monoamine oxidase gene in the recombinant broad-spectrum metarhizium is knocked out, so that the concentration of tryptamine in a broad-spectrum metarhizium robertsii is significantly increased, the insecticidal efficiency is further obviously increased, and the half lethal time LT50 of the wild-type broad-spectrum metarhizium is shortened to 6.136+ / -0.488 days from 7.33+ / -0.445 days; furthermore, the recombinant broad-spectrum metarhizium is harmless to the environment, good in biological safety and non-toxic to human beings.