Reagent kit for monoamine oxidase MAO single-reagent measurement
A technology of monoamine oxidase and kit, applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral characteristic measurement, etc., to achieve the effect of simple operation, convenient use, and easy promotion and application
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Embodiment 1
[0059] Embodiment 1: the preparation of the single reagent of kit
[0060] The present application exemplifies the preparation of three single-reagent kits, the preparation raw materials, preparation steps and the obtained components of the three single-reagents are:
[0061] 1) Preparation of reagents:
[0062] Required raw materials
[0063] name molecular weight purity Manufacturer Tris-base 121.1 99.9% Beijing Xinjingke Biotechnology Co., Ltd. NaCl 58.44 Analytical pure Beijing Yili Fine Chemicals Co., Ltd. NaN3 65.01 99% Merck EDTA-2Na 372.24 BSA 98.0% Amresco ADP—K 501.3 TritonX—100 647 99.0% Beijing Xinjingke Biotechnology Co., Ltd. α-ketoglutarate 146.1 99.5% Beijing Xinjingke Biotechnology Co., Ltd. Benzylamine 107.15 99.5% across D—glucose 180.16 99.8% Beijing Xinjingke Biotechnology Co., Ltd. GLDI 237u / mg Toyobo NADH 709.9 roche GDH ...
Embodiment 2
[0106] Embodiment 2: the method and step of detecting MAO in the sample with kit
[0107] 1) The detection parameters are:
[0108] Temperature 37°C
[0109] Wavelength 340nm(main)&405nm(secondary)
[0110] Cuvette light path 1.0cm
[0111] Test Method Rate Method
[0112] Reaction Direction Negative Reaction
[0113] 2) The detection steps are:
[0114] First adjust to zero with water for calibration; then add 0.018ml of sample; then add 0.18ml of reagent, incubate at 37°C for 4 minutes, test for 2 minutes, and calculate the change of the average absorbance per minute (ΔA / min) during the test period. The theoretical K value is -1740.
[0115] 3) Normal reference value of MAO in serum: 0-12U / L
Embodiment 3
[0116] Embodiment 3: the anti-interference ability of detection kit I, II, III
[0117]Simultaneously, the performance of 3 kinds of single kits configured in Example 1, i.e. kits I, II, and III, is detected as follows:
[0118] 1. Anti-interference experiment of the kit
[0119] The samples to be tested containing ascorbic acid, bilirubin, and Hb of different concentrations were configured according to conventional techniques in the art respectively, and kits I, II, and III were used to detect the activity of MAO in the sample in the detection instrument, thereby detecting the effects of the three kits on each The anti-interference ability of a kind of interfering substances (each sample was performed in triplicate, and the average value was taken). Test result (mean value) is as shown in table 1:
[0120] Table 1
[0121]
[0122] The results in Table 1 show that when ascorbic acid ≤ 50mg / dL, bilirubin ≤ 40mg / dL or Hb ≤ 200mg / dL, it does not affect the detection of MAO...
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