Leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe as well as synthetic method and biological application thereof

A technology of leucine aminopeptidase and monoamine oxidase, which is applied in the field of fluorescent probes, can solve the problems of low sensitivity and poor specificity of fluorescent probes, and achieve stable fluorescent signals, high selectivity, and good staining effects
CN110746410AActive Publication Date: 2020-02-04HUNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUNAN UNIV
Publication Date
2020-02-04

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Abstract

The invention discloses a leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe as well as a synthetic method and biological application thereof. The adopted syntheticmethod includes the following step: (1) synthesizing the novel leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe (NML); and the biological application comprises the following step: (1) applying the probe to biological imaging and serum detection. The leucine aminopeptidase and monoamine oxidase-responsive fluorescent probe is synthesized for the first time, and the problem of low specificity of a traditional single-enzyme fluorescent probe is overcome; the fluorescent probe has near-infrared fluorescence properties, the background fluorescence signal can be effectively reduced, and the sensitivity of the probe can be improved; and the fluorescent probe has a good staining effect on living cells and high staining efficiency, and can detect cell endogenous leucine aminopeptidase and monoamine oxidase.
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Description

technical field

[0001] The invention belongs to the technical field of fluorescent probes, and relates to a near-infrared fluorescent probe activated by leucine aminopeptidase and monoamine oxidase, a synthesis method and a biological application. Background technique

[0002] Liver diseases such as hepatitis, cirrhosis and liver cancer are major diseases worldwide. Currently, millions of people die from liver disease every year. The best treatment for liver disease today is accurate diagnosis and treatment before it gets worse. However, similar symptoms in the early stages of different liver diseases limit the accurate diagnosis of liver diseases. Standard methods for liver disease diagnosis, such as histopathological examination and molecular medical imaging, are often ineffective in the middle and late stages of the disease, which often leads to delays in diagnosis. Therefore, there is an urgent need to develop a simple and accurate method to diagnose and differentiate...

Claims

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