2702 results about "Endogeny" patented technology

The present invention relates to transmissible elements (e.g. conjugative plasmids, phage and phagemid) which are engineered to express molecules of interest (MOIs) and a pathway modification system (PMS) which is able to introduce mutations in target endogenous genes in a recipient bacterium, or is able to produce nucleic acids which inhibit or increase expression of target endogenous genes in a recipient bacterium, or is able to express peptide inhibitor molecules which inhibit a target endogenous protein in a recipient bacterium. The invention relates to host cells containing the transmissible elements, to pharmaceutical compositions containing such transmissible elements or host cells, and their use in the treatment of various diseases.

The invention discloses a bionic nanofiber-hydrogel composite biological scaffold as well as a preparation method and application thereof. The bionic nanofiber-hydrogel composite biological scaffold is a composite scaffold for simulating a gray-white matter partition structure of a spinal cord tissue, and comprises bionic nanofibers and a bionic hydrogel material, wherein the bionic nanofibers are arranged in an oriented manner and simulate a white matter ordered structure around the spinal cord, and the bionic hydrogel material is combined with the bionic nanofibers and simulates gray matter in the center of the spinal cord. According to the bionic nanofiber-hydrogel composite biological scaffold, the biological activity of loaded stem cells or adult cells can be improved, the problem that a large number of transplanted exogenous cells die due to unbalanced oxidative stress generated after tissue damage can be solved, and it is guaranteed that the transplanted cells reside in a damaged area and play a role; therefore, the spinal cord microenvironment is improved, proliferation and differentiation of endogenous neural stem cells are promoted, functional recovery after spinal cord injury is finally promoted, and great potential is achieved in spinal cord injury treatment.

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention relates to the technical field of detection kits, in particular to a detection kit for neutrophil gelatinase-associated lipocalin and a preparation method of the detection kit. The invention provides a detection kit for neutrophil gelatinase-associated lipocalin. The detection kit comprises an independently packaged reagent R1 and an independently packaged reagent R2, the reagent R1 comprises an HBR series specific blocking agent; the reagent R2 comprises carboxyl latex microspheres coupled with an NGAL antibody; the carboxyl content of the carboxyl latex microspheres is greater than 100 [mu] eq / g, and the particle size is 100-400 nm. According to the kit, an HBR series specific blocking agent is used as a component of the reagent R1, so that endogenous interference is reduced, and false positive and negative detection results of a sample are avoided. Furthermore, the latex with proper particle size is selected to be coupled with the polyclonal antibody, and the sealing condition is optimized, so that the method has the advantages of wide linearity, good analysis sensitivity, simplicity in operation, controllable batch difference and controllable cost.

Disclosed herein is a method of determining subject sensitivity to a WEE1 inhibitor, comprising obtaining or having obtained a biological sample from the subject, and performing or having performed at least one assay on the biological sample to determine if the subject has an altered function of CCNE1. Also disclosed are methods of treating cancer with a WEE1 inhibitor, comprising identifying a subject having (a) the cancer and (b) endogenous or altered function of CCNE1; and administering an effective amount of the WEE1 inhibitor, or a pharmaceutically acceptable salt thereof, to the subject.

The invention discloses a camel source single-domain antibody GH12 for recognizing a conformation B cell epitope in clostridium difficile glutamate dehydrogenase and application of the camel source single-domain antibody GH12. The nucleotide sequence of the camel source single-domain antibody GH12 is shown as SEQ ID NO: 1, and the amino acid sequence of the camel source single-domain antibody GH12 is shown as SEQ ID NO: 2. The camel source single-domain antibody GH12 disclosed by the invention has the advantages of strong specificity, high sensitivity, good accuracy and the like, and it is proved that the camel source single-domain antibody GH12 can be used for detecting recombinant expressed CD-GDH and endogenous CD-GDH by enzyme-linked immunosorbent assay (ELISA), protein dot blot hybridization (DB) and other methods.

The invention discloses a gene and a protein for adjusting tobacco polyphenol aroma substances and application of the gene and the protein. The gene is a tobacco NtMYB28 gene, and the CDS sequence of the tobacco NtMYB28 gene is as shown in SEQ ID No. 1. The invention further discloses application of the tobacco NtMYB28 gene in regulating the content of endogenous polyphenol substances in tobacco and regulating and controlling the metabolic pathway of phenylpropane in tobacco. The key gene NtMYB28 capable of simply and effectively adjusting the content of polyphenol substances in tobacco and the metabolic pathway of phenylpropane is found, and the gene can be applied to efficient cultivation of new high-aroma tobacco materials and development and utilization of new high-value functional tobacco germplasm.

The invention discloses an oligopeptide composition for inhibiting inclusion body formation and promoting Gag-Like protein nanoparticle self-assembly, the oligopeptide composition comprises a C-terminal oligopeptide and an N-terminal oligopeptide, the amino acid sequence of the N-terminal oligopeptide is as shown in SEQ ID NO: 1, and the amino acid sequence of the C-terminal oligopeptide is as shown in SEQ ID NO: 2. The oligopeptide composition can effectively avoid formation of a prokaryotic expression protein inclusion body and / or promote dissolution of prokaryotic expression protein, the oligopeptide composition is further utilized to be combined with HIV-1p24 and human endogenous retrovirus K capsid protein CA, nanoparticles are prepared respectively, and the oligopeptide composition can be used as a carrier for antigen surface display or drug transportation. The nanoparticles formed by the HIV-1 capsid protein p24 and the human HERV K CA have cavities which are obviously larger than those of ferritin, so that the nanoparticles have higher loading capacity; as an antigen display platform, the antibody also has good stability and wide applicability.

The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1 and application thereof, and relates to the technical field of biology. The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1. The nucleotide sequence of the enhancer RNA molecule MZGAe1 is as shown in SEQ ID NO: 1; meanwhile, the invention further provides a specific sgRNA sequence for activating the molecule in a targeted manner and a recombinant vector of the specific sgRNA sequence. Expression of endogenous MZGAe1 of cells is specifically activated by adopting a CRISPR activation technology, and conversion of mouse embryonic stem cells to bicellular-like cells can be efficiently promoted. By providing a brand-new endogenous targeted MZGAe1 accurate activation tool, the efficient transformation of wild mouse embryonic stem cells to bicellular cells can be realized only under the condition of endogenous activation of single enhancer RNA molecule MZGAe1, the proportion is at least increased by 5%, the expression of a totipotent marker gene ZSCAN4 is activated, and the expression of the totipotent marker gene ZSCAN4 is promoted. And an efficient and specific brand-new research tool and scheme are provided for researching zygote genome activation, embryonic development early events and cell reprogramming.

The invention features compounds (e.g., macrocyclic compounds) capable of modulating biological processes, for example through binding to a presenter protein (e.g., a member of the FKBP family, a member of the cyclophilin family, or PIN1) and a target protein (e.g., a eukaryotic target protein such as a mammalian target protein or a fungal target protein or a prokaryotic target protein such as a bacterial target protein). These compounds bind endogenous intracellular presenter proteins, such as the FKBPs or cyclophilins, and the resulting binary complexes selectively bind and modulate the activity of intracellular target proteins. Formation of a tripartite complex among the presenter protein, the compound, and the target protein is driven by both protein-compound and protein-protein interactions, and both are required for modulation of the targeted protein's activity. In some embodiments, the compounds of the invention “re-program” the binding of the presenter proteins to protein targets that either do not normally bind to the presenter protein (e.g., do not show detectable binding in mammalian cells absent the compound). In some embodiments, provided compounds “re-program” presenter protein binding to greatly enhance interaction with a particular target with which it may have some interaction absent the compound. Interactions achieved through such reprogramming result in an ability to modulate the activity of these new targets.

The invention discloses a transcranial electrical stimulation control method and system for enhancing working memory, and the method comprises the steps: collecting and preprocessing a multi-channel endogenous electroencephalogram signal of a target object during the execution of a cognitive task, and obtaining a to-be-analyzed electroencephalogram signal; aligning the to-be-analyzed electroencephalogram signal with a behavior expression label generated by the target object during execution of the cognitive task to obtain an associated signal; according to the correlation signal, obtaining a channel pair with the highest phase synchronism between the forehead lobe and the apical lobe of the target object as a target channel pair; based on the associated signal data of the target channel pair, extracting frequency band features to generate stimulation parameters of radial and cranial electrical stimulation; and outputting transcranial electrical stimulation according to the stimulation parameters, and collecting endogenous electroencephalogram signals during stimulation in real time, so that the stimulation parameters are dynamically adjusted, and closed-loop regulation and control on the brain area of the target object are realized. Therefore, by the adoption of the method and device, accurate control over the endogenous phase difference between the prefrontal lobe and the apical lobe can be achieved, and therefore the work memory performance is enhanced.

The invention discloses a medicine for targeting senescent cells to stimulate endogenous immunity and application of the medicine, and relates to the field of biological medicine. The medicine provided by the invention can mediate the interaction between senescence cells and immune cells, enhance the endogenous immune function, and achieve the purposes of effectively removing senescence cells and improving senescence-related diseases. According to the functional molecule for targeted aging cell immune sensitization, one end of the molecule targets an aging cell membrane molecule, glutamic acid is used for marking the surface of an aging cell, coupling between an endogenous immune cell and the aging cell is mediated, and adhesion and removal of the aging cell by the immune cell are promoted. The molecule can regulate the immune microenvironment of senescence tissues, effectively reduce the number of positive senescence cells of senescence-related beta-galactosidase, reverse fibrosis and improve natural senescence phenotypes.

A method and apparatus for treatment of hypertension and heart failure by increasing secretion of endogenous atrial hormones by pacing of the heart. Pacing is done during the ventricular refractory period resulting in premature atrial contraction that does not result in ventricular contraction. Pacing results in the atrial wall stress, peripheral vasodilation, ANP secretion. Concomitant reduction of the heart rate is monitored and controlled as needed with backup pacing.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention belongs to the technical field of battery dressings, and particularly relates to a wearable magnesium battery self-powered dressing for chronic wound healing and a preparation method and application thereof. A flexible conductive carbon cloth coated with a viologen-based covalent organic framework material is used as a battery positive electrode, a magnesium sheet is used as a negative electrode, polyacrylamide hydrogel is used as a solid electrolyte, and the flexible wearable self-powered dressing is assembled. The formed electrode can effectively input electrical stimulation to the wound surface, the dressing can simulate an endogenous electric field in the wound, induce migration of fibroblasts, regulate the immune microenvironment of the wound and inhibit inflammatory response, polyacrylamide is used as gel electrolyte, and the dressing has good hygroscopicity, can absorb exudate at the wound surface and keep a wet healing environment, and can be applied to the wound healing. The chronic wound healing of the rat is effectively promoted. The self-powered dressing prepared by the invention provides a reliable electrotherapy method for chronic wound healing.

A high-throughput microfluidics-based fluorescence activated droplet sorting (FADS) system provides activation, sorting, and feeder cell-free expansion of NK cells that are enriched for killing activity. The NK cells are activated and identified based on their ability to kill target cells, such as tumor cells, without prior sensitization. The diversity of endogenous NK cells can be overcome to allow selection of a highly effective population of cells for therapeutic use, such as in the treatment of cancer.

The invention relates to the technical field of plant genetic engineering, in particular to a promoter and application thereof in orchid plants. The invention provides an orchid endogenous promoter which is a powerful constitutive promoter and can drive a target gene to be powerfully expressed in various tissues such as roots, stems, leaves and flowers of an orchid. Compared with a CaMV 35S promoter, the promoter has the advantages that the driving efficiency of the gene in orchid plants is greatly improved, an important tool is provided for molecular breeding of plants, and the promoter has a relatively good application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.

Disclosed are methods, means, and compositions of matter useful for the stimulation of angiogenesis directly by administration of membrane vesicles, such as fibroblast-derived exosomes, and / or through induction of angiogenic cytokines from blood cells contacted with fibroblast-derived exosomes. The invention provides means of treating conditions in which angiogenesis is beneficial through local or systemic administration of exosomes, including those derived from fibroblasts, wherein the fibroblasts are cultured under basal conditions or conditions of hypoxia. In other embodiments exosomes derived from fibroblasts are utilized to augment endogenous regenerative processes, such as hematopoiesis, angiogenesis and neurogenesis, as well as augment regenerative processes stimulated by administration of exogenous therapeutics such as cells, growth factors, or genes.

The application discloses a specific palmitoyl transferase inhibitor and application thereof, and an active ingredient of the palmitoyl transferase inhibitor is 4-(3-(2-(trifluoromethyl)-9H-thioxanthene-9-ylidene)propyl)-1-piperazine ethanol or a salt thereof. Current palmitoylation inhibitors, 2-bromohexadecanoic acid (2-BP), cerulenin and tunicamycin can all inhibit protein palmitoylation in cells, but all are not selective. And the existing palmitoylation inhibitors are similar to endogenous fatty acids, and inevitably affect lipid metabolism in the body, which limits the clinical application. The application discloses, for the first time, the application of 4-(3-(2-(trifluoromethyl)-9H-thioxanthene-9-ylidene)propyl)-1-piperazine ethanol as a specific palmitoyl transferase inhibitor, and the palmitoyl transferase inhibitor is better in specificity and reversible in inhibition.

The invention discloses a Taq DNA polymerase mutant as well as a preparation method and application thereof, and relates to the technical field of biology. The Taq DNA polymerase mutant is mutation on the basis of a wild type Taq DNA polymerase amino acid sequence as shown in SEQ ID NO.1, and comprises at least one of amino acid mutation L345E, L352D and S438M. The Taq DNA polymerase mutant has excellent tolerance to urea, and solves the dual technical problems that complex biological samples containing endogenous inhibitors such as urea can be efficiently amplified, and the Taq DNA polymerase mutant is compatible with difficult PCR reactions which take urea as an additive to optimize a high GC template and the like. Therefore, the mutant is a molecular biology tool enzyme which is wider in applicability and more stable and reliable in performance.

The present disclosure provides mouse models comprising an endogenous Stathmin2 (Stmn2) gene that comprises a human exon 2a polynucleotide sequence.

In some aspects, the disclosure relates to compositions and methods useful for inhibiting SOD1 expression in cells (e.g., cells of a subject). In some embodiments, the disclosure describes isolated nucleic acids engineered to express an inhibitory nucleic acid targeting endogenous SOD1 and an mRNA encoding a hardened SOD1 protein. In some embodiments, compositions and methods described by the disclosure are useful for treating Amyotrophic Lateral Sclerosis (ALS) in a subject.

The invention relates to a method and application for positively regulating the defense response of plants to pests, and the method comprises the following steps: the plants are contacted with small RNA (Ribonucleic Acid) to enhance the resistance of the plants to the pests, and the small RNA comprises plant endogenous 22nt-siRNA. Researches find that the endogenous 22nt-siRNA of the plant can improve the resistance of the plant to phytophagous insects, and the 22nt-siRNA can specifically target a field phytophagous insect gene while enhancing the resistance of the plant, has the biological prevention and control advantage of no environmental pollution, and can be used for preventing and treating the phytophagous insects. Therefore, the method plays an important role in forward regulation of defense response of plants to pests and prevention and control of plant pests.

The invention discloses a multifunctional coating capable of forming a long-term stable endogenous electric field to promote antibiosis, and a preparation method and application thereof, and belongs to the technical field of biological functional modification. According to the coating, through the elaborately designed Ta / Ag heterostructure, long-term stable generation of an endogenous electric field is achieved, the antibacterial performance is enhanced, osseointegration is promoted, and the problem of drug resistance is avoided; the coating does not need external electrical stimulation, so that the operation process is simplified, and the clinical application risk is reduced; due to the Ta material, the osseointegration capacity is improved, and Ag has broad-spectrum and efficient antibacterial performance. The TC4-Ta / Ag group has excellent charge storage capacity and a stable electric field, and the long-term osseointegration effect is remarkably improved; according to the preparation method of the coating, the coating which is stable in structure and not prone to falling off can be obtained, the long-term stable antibacterial performance is kept, the coating has wide application prospects in the field of titanium alloy bone implants, a new solution is provided for orthopedic surgery and implant treatment, and the treatment effect and life quality of patients are improved.

The invention discloses clostridium butyricum as well as a culture method and application thereof, belongs to the field of feed additives, and aims to solve the problems that a controllable fattening period is shortened and the efficacy of the clostridium butyricum cannot be completely exerted due to the fact that a wide range of clostridium butyricum needs a relatively long adaptation period for an endogenous intestinal environment of an animal. The clostridium butyricum from the North Naa Biotechnology Co., Ltd. Is cultured by simulating an animal endogenous environment in vitro, and after the clostridium butyricum which completely adapts to animal endogenous is obtained, the screened animal endogenous clostridium butyricum is coated by adopting a coating technology, so that the clostridium butyricum is not damaged by a strong acid environment in an animal stomach bag; the clostridium butyricum strain has the advantages that the clostridium butyricum strain can be used for preparing animal endogenous clostridium butyricum, a large quantity of animal endogenous clostridium butyricum can reach intestinal tracts and be released, and the animal endogenous clostridium butyricum can quickly adapt to animal intestinal environment due to higher adaptability of the strains, and can quickly form efficient colonization and functional expression under the condition of almost no adaptation period. The feed additive can be used as a biological feed additive.

The invention discloses a nano-carrier based on charge crosslinking, a preparation method of the nano-carrier and application of the nano-carrier as a tumor chemotherapy drug. Wherein the nano-carrier is formed by assembling two polymer chains with positive and negative charges through electrostatic interaction, a precursor ligand on a polymer chain B releases an organic ligand under exogenous or endogenous stimulation, and competes for metal ions on a polymer chain A with positive charges to generate a metal complex in situ, and meanwhile, the electrostatic interaction of the nano-carrier is weakened, so that the metal complex is formed. The dissociation of the nano-carrier is caused, a macromolecular chain B containing an organic ligand is released, and the effect of tumor chemotherapy or immunoregulation is achieved. The method is simple to operate, short in time consumption and mild in reaction condition; the material is good in biocompatibility and high in safety degree. Pharmacodynamic experiments in some embodiments show that the nano-carrier can effectively kill the breast cancer and trigger cellular immunogenic death, meanwhile, tumor cell metastasis is inhibited, and a new thought and means are provided for the clinical problem of breast cancer lung metastasis urgently needing to be solved at present.

The invention relates to an application of a protein coded by circular RNA circVEGFC in an esophageal squamous cell carcinoma diagnosis product. The invention provides a protein circVEGFC-182aa translated by a circular RNA (Ribonucleic Acid) molecule circVEGFC and application of the protein circVEGFC-182aa. It is found that the expression of a circular RNA molecule circVEGFC (namely hsacirc0004496) in esophageal squamous cell carcinoma tissue is remarkably increased, the circular RNA molecule circVEGFC participates in the malignant progression of esophageal squamous cell carcinoma, the circVEGFC can encode a protein containing 182 amino acids, namely circVEGFC-182aa, the protein is subjected to endogenous expression in esophageal squamous cell carcinoma, and the invasion and migration ability of esophageal squamous cell carcinoma is promoted; the result shows that the circVEGFC plays a biological function mainly through the specific protein circVEGFC-182aa coded by the circVEGFC, so that the circVEGFC-182aa can be used as a diagnosis marker of esophageal squamous cell carcinoma to assist diagnosis.

The invention discloses interference engineering bacteria for blocking early infection of animal parasitic nematodes and application, and belongs to the field of animal parasitic disease prevention and treatment. According to the interference engineering bacteria disclosed by the invention, the HCON00038080 (cyp-22a1) gene of haemonchus contortus is specifically silenced by expressing double-stranded RNA (dsRNA), so that the biosynthesis of endogenous bile acid-like steroid hormone of the haemonchus contortus is inhibited, the activation of infectious larvae is influenced, and the infection establishment process of the infectious larvae is blocked. The invention can be used for preventing early infection of haemonchus disease of ruminants such as cattle, sheep, camels and the like and the phenomenon of spring / perinatal high tide, has higher application value for prevention and control of haemonchus disease, and has reference value for prevention and control of parasitic nematode disease of other animals.