361 results about "Infrared fluorescence" patented technology

The invention discloses a thyroid intraoperative real-time navigation method and a thyroid intraoperative real-time navigation system based on multi-mode optical fusion. The thyroid intraoperative real-time navigation method comprises the following steps: outputting visible light through an endoscope and coupling light waves of a narrow-band multispectral light source to irradiate an operative field, exciting parathyroid glands to generate near-infrared fluorescence, and receiving reflected visible light, split light, near-infrared fluorescence and laser speckle signals through an endoscope probe. And separating the composite optical signal into four channels, and respectively generating an anatomical structure color image, a blood vessel spectroscopic image, a parathyroid gland near-infrared fluorescence image and a laser speckle image. Performing decorrelation processing on the laser speckle image to generate a blood flow dynamic pseudo-color decorrelation speckle image; an anatomical structure, a blood vessel center line, a parathyroid gland contour and blood flow dynamic feature points are extracted through a multi-modal registration technology, after affine transformation space alignment is conducted, a comprehensive imaging map containing the anatomical structure, blood vessel distribution and parathyroid gland function marking information is generated through a wavelet fusion algorithm, and intraoperative multi-dimensional real-time tissue navigation is achieved.

The invention provides a fluorescence-magnetic particle image fusion method which can be applied to the technical field of medical image processing. The method comprises the following steps: performing two-dimensional convolution processing on a registered two-dimensional near-infrared fluorescence image to obtain a multi-channel near-infrared fluorescence extended feature map, and performing initial fusion on the multi-channel near-infrared fluorescence extended feature map and a registered three-dimensional magnetic particle cross-sectional image to obtain a three-dimensional near-infrared fluorescence extended feature map; performing multi-round three-dimensional convolution processing on the three-dimensional near-infrared fluorescence extended feature map to obtain a multi-scale near-infrared fluorescence feature map, and performing multi-round three-dimensional convolution processing on the registered three-dimensional magnetic particle cross-sectional image to obtain a multi-scale magnetic particle feature map; and based on an adaptive cross attention mechanism, performing multi-round feature map fusion on the multi-scale near-infrared fluorescence feature map and the multi-scale magnetic particle feature map on the same scale, and performing filtering convolution processing on a multi-round feature map fusion result to obtain a three-dimensional fluorescence-magnetic particle fusion image.

The invention relates to a microscope system, in particular to a confocal microscope system of a short-wave infrared band. The system comprises a bright / dark field microscopy subsystem, a photon emission microscopy subsystem, a short wave infrared laser scanning confocal microscopy subsystem, a fluorescence confocal microscopy subsystem and a control module. According to the system provided by the invention, the short-wave infrared fluorescence lifetime curve can be obtained through the electro-luminescence emission and / or photoluminescence emission of the semiconductor device in the integrated circuit, so that the defect type of the semiconductor can be judged. Besides, the system adopts a multi-subsystem optical path multiplexing structure in structural design, short-wave infrared fluorescence generated by exciting a defect or a failure point is collected while observation is facilitated, high integration level is achieved, and observation, positioning and failure analysis of the defect or the failure point can be achieved only by using a single system provided by the invention.

The invention discloses an ultra-wide-spectrum endoscopic imaging device capable of being used for short-wave infrared fluorescence imaging, which is used for in-vivo minimally invasive imaging and comprises an illumination module, a wide-spectrum image transmission module, an imaging module and an image processing module. The illumination module consists of a light source and a light guide optical fiber, and is used for generating illumination light from ultraviolet to short wave infrared band and transmitting the illumination light to the wide-spectrum image transmission module; the wide-spectrum image transmission module consists of a few-interface optical imaging channel and a light guide illumination channel, and is used for illuminating an imaging target, exciting target fluorescence and collecting image signals from ultraviolet to short-wave infrared bands; the imaging module is composed of an optical filter and an imaging camera, and the imaging camera can be a wide spectral response image sensor or can be formed by combining cameras working in different wavebands and is used for converting optical images in different wavebands into image data; and the image processing module is used for registering and fusing image data of different wavebands in real time and displaying the image data.

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention relates to the technical field of medical chemistry, in particular to a bridge oxygen site regulatively modified rhodamine anti-cancer fluorescent compound as well as a preparation method and application thereof. The invention develops the rhodamine anti-cancer fluorescent compound of which the meso site is functionalized by tetraarylethene and the bridge oxygen site can be regulated and modified, the fluorescent compound has adjustable red light-near infrared fluorescence emission and excellent fluorescence quantum yield, and the quantum yield can be as high as 99%; the fluorescent compound specifically targets mitochondria, and mainly inhibits growth of tumor cells by inducing para-apoptosis; under irradiation of 655 nm near-infrared laser, ferroptosis is further triggered, efficient treatment of tumor cells is achieved through chemical-photodynamic synergistic effect, and a new treatment thought is provided for overcoming tumor drug resistance.

The invention belongs to the technical field of analysis and detection, and particularly relates to a beta-galactosidase response type hemicyanine near-infrared fluorescent probe, a preparation method thereof and application of the beta-galactosidase response type hemicyanine near-infrared fluorescent probe as a tumor organ imaging probe. The near-infrared fluorescent probe provided by the invention takes a water-soluble hemicyanine skeleton as a fluorophore, so that the biocompatibility of the near-infrared fluorescent probe is improved; based on the principle that beta-galactose can be selectively cut by beta-galactosidase, a galactose structure with a self-leaving group is used as a response group, selective quick response to the beta-galactosidase is achieved, and high sensitivity to the beta-galactosidase is shown. The near-infrared fluorescent probe provided by the invention can emit near-infrared fluorescence with long wavelength, has lower background fluorescence and good penetration depth, and can be directly used for detecting organoids containing the matrigel without eliminating the matrigel, so that the organoids are prevented from being damaged or additional experimental variables are prevented from being introduced in the process of eliminating the matrigel.

The invention belongs to the technical field of antitumor drugs, and particularly discloses a preparation method and application of a compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment. The compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment is shown in the following structural formula: # imgabs0, the molecular skeleton of the compound contains a triphenylamine unit and an N, N-dimethylaniline fragment, intramolecular rotation limitation and intramolecular vibration limitation can be generated, and obvious aggregation-induced emission (AIE) performance is shown. The function-integrated phototherapy agent has high active oxygen generation efficiency, photothermal conversion efficiency and near-infrared fluorescence quantum yield, and is beneficial to obtaining ideal photothermal-photodynamic therapy effect for deep tumors. The phototherapy agent is novel in structure, the preparation method is easy in raw material obtaining, the synthesis condition is mild, the preparation method is simple, purification is convenient and fast, implementation is easy, and the phototherapy agent has great application prospects.

The invention discloses an ultra-sensitive viscosity fluorescent probe based on a carbon-nitrogen double bond. The chemical structural formula of the probe is as follows: # imgabs0 #. The fluorescent probe is synthesized through Schiff base reaction, and is simple to operate and easy to synthesize. The probe can freely rotate at carbon-carbon double bonds and carbon-nitrogen double bonds, and when the probe is in high viscosity, free rotation of molecules is limited, so that twisted intramolecular charge transfer is inhibited, fluorescence of the probe is remarkably enhanced, and 650 nm near-infrared fluorescence is emitted under the excitation wavelength of 600 nm. In addition, the probe has ultrahigh sensitivity, excellent selectivity, excellent light stability and pH tolerance, and has huge application prospects in the technical fields of analytical chemistry, life science, biomedical treatment and the like.

The invention provides a high-luminous-efficiency full-spectrum light source manufacturing method and an LED device. The method comprises the steps that a metal support is provided; a light-emitting chip is welded to the metal support so that the whole metal support can form a channel, and the wave band range of the light-emitting chip is 400 nm to 480 nm; infrared fluorescent powder and glue are evenly mixed, bubbles are removed, infrared fluorescent powder packaging glue is obtained, the infrared fluorescent powder packaging glue is dispensed above the light-emitting chip, first fluorescent glue is formed, and the waveband range of the infrared fluorescent powder is 680-780 nm; mixing white light combined fluorescent powder with glue to obtain white light combined packaging glue, coating the white light combined packaging glue on the first fluorescent glue, and drying the white light combined packaging glue to obtain the full-spectrum light source with high luminous efficiency.

The invention discloses a near-infrared confocal microscopic imaging system and a near-infrared confocal microscopic imaging method, and relates to the technical field of optical microscopic imaging. The method comprises the following steps that near-infrared exciting light sequentially passes through a beam expanding system, a liquid lens and a confocal scanning light path to irradiate a to-be-detected sample so as to generate a near-infrared fluorescence signal, and the liquid lens is used for adaptively adjusting the focal length and wavefront morphology according to the depth of the to-be-detected sample; a photomultiplier detects the near-infrared fluorescence signal after non-focus scattered light is filtered out through the confocal scanning light path and the pinhole, and a fluorescence signal image is obtained; and according to the fluorescence signal image, carrying out iterative optimization on the focal length and phase modulation parameters of the liquid lens to maximize the image sharpness or the signal-to-noise ratio and realize the three-dimensional microscopic imaging of the deep tissue. According to the near-infrared confocal microscopic imaging method provided by the invention, deep near-infrared microscopic imaging with low phototoxicity, low cost and high resolution can be realized.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The utility model discloses an imaging system. The imaging system comprises a light source, an endoscope connected with the light source, a second dichroscope, a visible light camera and a near-infrared camera. The light source comprises an excitation light source, an illumination light source and a first dichroscope used for combining light beams emitted by the excitation light source and the illumination light source. A light beam emitted by the light source is irradiated to a target object through the endoscope, so that the target object reflects visible light and is excited to emit near-infrared fluorescence, and the endoscope is used for collecting the visible light and the near-infrared fluorescence from the target object; the second dichroscope is arranged on the optical axis of the endoscope and is used for dividing the light from the endoscope into visible light and near-infrared fluorescence; and the visible light camera and the near-infrared camera are respectively used for imaging the visible light and the near-infrared fluorescence from the second dichroscope. In this way, the light beams emitted by the excitation light source and the illumination light source can irradiate the same position of the target object, and imaging of near-infrared fluorescence and visible light of the same scene is achieved.

The present invention relates to a fluorescent compound based on new indocyanine green IR820 and its preparation and application. The molecular structure of the fluorescent compound is shown in formula (1): Compared with the prior art, the fluorescent probe of the present invention has near-infrared fluorescence emission, good tumor targeting, biocompatibility, low cytotoxicity, and strong biological tissue penetration. It can be used for targeted fluorescence imaging of tumor cells and living tumors, and is expected to be further used in fluorescence surgical navigation.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The invention discloses a difunctional near-infrared fluorescent probe as well as a synthesis method and application thereof. The structural formula of the fluorescent probe is shown as a formula (I). The fluorescent probe DTPD provided by the invention can be used for fluorescent quantitative detection of hypochlorous acid and viscosity, and the fluorescent probe can realize detection of hypochlorous acid with high selectivity in an aqueous solution and is not interfered by other coexisting substances. Besides, the near-infrared fluorescent probe provided by the invention has relatively strong organism penetrability in a near-infrared region, can also reduce the interference of autofluorescence of organisms, and has a good cell imaging effect. And # imgabs0 #.

The structural formula of the oxygen-rhodamine near-infrared fluorescent dye is shown as a formula (a) or a formula (b), and R1 and R2 are respectively selected from hydrogen, alkyl or alkenyl; r3 and R4 are respectively selected from hydrogen, alkyl and alkenyl; r5-R9 are respectively selected from hydrogen, alkyl, methoxyl, ethyoxyl, halogen, carboxyl, sulfonic acid group, ethylene glycol group, hydroxyl or amino, and N is an integer greater than or equal to 0. The invention also discloses a preparation method and application of the fluorescent dye. The compound is novel in structure, excellent in performance, simple in preparation method and good in product application prospect.

The present invention provides a large Stokes shift near-infrared fluorescent probe for detecting HClO, its preparation method and biological imaging application, belonging to the field of bioanalysis technology. Based on the strategy of modifying the substituents at the 3,6 positions of xanthene, the present invention constructs a large Stokes shift near-infrared fluorescent probe for specifically recognizing HClO. Optical experiments show that the emission wavelength of the probe for detecting HClO is 776 nm, the Stokes shift reaches 148 nm, with strong selectivity, high sensitivity, and a detection limit as low as 4.95 nM, which can greatly reduce the interference of background fluorescence in biological imaging applications. The fluorescent probe of the present invention has almost no cytotoxicity and can be applied to the detection and imaging of HClO in living cells MCF-7 and rat liver tissues, with a tissue penetration depth of 140 μm.

The application discloses a near-infrared fluorescent powder and a light-emitting device. The near-infrared fluorescent powder comprises a composition formula LiA a Sc 1‑a‑p‑d‑ c D d P p An inorganic compound of EO4:cCr, wherein A elements include one or two of Li, Na and K, D elements include two of Ga, In, Al, Ce and Bi, and E elements include two or three of Hf, Ge and Si; wherein 0

This invention discloses a method for preparing HVC NPs for the diagnosis and treatment of Parkinson's disease and their applications. The probe HV in the HVC NPs responds to H2O2 and viscosity, utilizing near-infrared fluorescence imaging capabilities to achieve real-time monitoring of Parkinson's disease biomarkers, helping us better understand the occurrence and development of Parkinson's disease. With the assistance of RVG29, the HVC NPs effectively cross the blood-brain barrier. The curcumin within them can reduce cell apoptosis, lower oxidative stress levels, scavenge excess reactive oxygen species, combat inflammation, and reduce α-synuclein aggregation, demonstrating effective anti-Parkinson's disease effects at the cellular and experimental animal levels. This nanomaterial provides a new strategy for integrated research on the diagnosis and treatment of Parkinson's disease.

The invention discloses a near-infrared fluorescence imaging agent of a targeted folate receptor, an imaging system and application of the imaging agent and the imaging system, and belongs to the technical field of biomedical treatment. The technical problem to be solved is to provide the folate receptor-targeted near-infrared fluorescence imaging system, an imaging agent in the system can specifically target the folate receptor FR-alpha, and the folate receptor-targeted near-infrared fluorescence imaging system is used in application scenes of lung cancer and ovarian cancer and can show higher TNR, display tumor boundaries in real time and reduce the positive edge cutting rate. According to the technical scheme, the near-infrared fluorescence imaging agent of the targeted folic acid receptor is characterized by comprising a compound of the targeted folic acid receptor, a solvent and pharmaceutically acceptable auxiliary materials, the structure of the compound of the targeted folic acid receptor is F-L-D, F is the structure of the targeted folic acid receptor, D is a near-infrared dye, L is a connexon, and L is an electron donor. The structural formula is shown in the specification or pharmaceutically acceptable salts thereof.

The present invention discloses a Cr 3+ -doped hexafluoride near-infrared fluorescent material and a preparation method thereof. The chemical composition formula of the material is: K2LiMF6:xCr 3+ , where M is one of Al, Ga or In, and x is the molar percentage coefficient of the doped Cr 3+ ions relative to the M 3+ ions, and 0 < x ≤ 0.09. The fluorescent material K2LiMF6:xCr 3+ disclosed by the present invention has a stable cubic double perovskite structure and can achieve broadband near-infrared emission with ultra-high thermal stability under visible light excitation. It can be used to prepare a broadband near-infrared light source device to realize the functions of night vision and vein imaging. The preparation method of the K2LiMF6:xCr 3+ material disclosed by the present invention is a hydrothermal method, which is carried out in an aqueous solution. This method has simple operation and mild reaction conditions and is suitable for large-scale industrial production.

The present invention discloses an LED light source for full-spectrum plant lighting, comprising a multi-channel bracket, an ultraviolet chip, a first blue light chip, a second blue light chip, a red light chip, a red light fluorescent glue layer, a near-infrared fluorescent glue layer, a first fluorescent glue layer, a second fluorescent glue layer and a multi-channel driver, wherein: the ultraviolet chip, the first blue light chip, the second blue light chip and the red light chip are arranged in series or in parallel on the upper surface of the bracket; the upper surface of the ultraviolet chip is coated with a red light fluorescent glue layer and a near-infrared fluorescent glue layer in sequence; the first fluorescent glue layer is dotted above the first blue light chip; the second fluorescent glue layer is dotted above the second blue light chip; the bracket is filled with transparent silica gel, and the multi-channel driver electrically connects and controls the current of the ultraviolet chip, the first blue light chip, the second blue light chip and the red light chip respectively.

The invention belongs to the field of molecular fluorescent probes, and particularly discloses a near-infrared fluorescent probe of quinone oxidoreductase 1 (NAD (P) H: quinone oxidoreductase 1, NQO1) with an in-situ covalent anchoring function and a preparation method of the near-infrared fluorescent probe. According to the probe, dicyanoisophorone is used as a core fluorophore, an aldehyde group is used as an NQO1 covalent anchoring group, and 3-methyl-3-(2, 4, 5-trimethyl-3, 6-dioxocyclohex-1, 4-diene-1-yl) butyrate is used as a recognition group of NQO1. The invention also provides an application of the near-infrared fluorescent probe with the in-situ covalent anchoring NQO1. Compared with other probes, the probe is simple in synthesis method and suitable for industrial production, and has high sensitivity, high selectivity and good biocompatibility in NQO1 detection. The fluorescent probe disclosed by the invention can realize covalent labeling and in-situ imaging functions after target NQO1 recognition response. Long-time in-situ tracking and imaging can be carried out on tumor cells and tissues by monitoring the NQO1 level, and a simple and effective method is provided for accurate detection of tumor disease related protease.

The present application relates to the technical field of nanoprobes, and particularly relates to a near-infrared fluorescence / magnetic resonance dual-mode probe and a preparation method and application thereof. The present application adopts a Brij98 amphiphilic molecule to wrap an IR824Et-PhDTA-Gd near-infrared fluorescence / magnetic resonance dual-response molecule to prepare the probe, so that the probe has an absorption wavelength of 650-850 nm, generates a fluorescence signal under excitation of 785 nm laser, and the generated fluorescence wavelength can reach 1000 nm, which is in a near-infrared region (780-1700 nm); the probe has good positive magnetic resonance response characteristics, and a longitudinal relaxation rate (r1) of the probe is 28 mM ‑1 s ‑1 , which is 7 times of that of a clinically used Magnevist; the probe can be applied in the fields of fluorescence and / or magnetic resonance imaging, and has a wide application prospect. The preparation method of the near-infrared fluorescence / magnetic resonance dual-mode probe is simple in operation and low in cost.

The invention relates to the technical field of biological detection, in particular to an HClO near-infrared fluorescent probe as well as a preparation method and application thereof. The structure of the HClO near-infrared fluorescent probe is shown in the specification. The emission wavelength of the near-infrared fluorescent probe developed by the invention is 750nm, and the near-infrared fluorescent probe has high selectivity, high sensitivity (the detection limit is as low as 49 nM) and quick response capability to HClO, and has low cytotoxicity and excellent in-vivo imaging capability, so that visual observation of HClO in a living Parkinson's disease model mouse body is successfully realized, and the near-infrared fluorescent probe has a wide application prospect. The method has great potential in the aspect of tracking HClO related pathological states in real time.

The invention belongs to the field of biomedical molecular images, particularly relates to an endoscope visible light image and near-infrared fluorescence image fusion method based on unsupervised deep learning, and aims to overcome the defects that splicing fusion of visible light images and near-infrared fluorescence images is inaccurate and low in speed and the like. The method comprises the following steps: acquiring an original visible light image and an original near-infrared fluorescence image as a training set of data, constructing and training a convolutional neural network based on unsupervised image fusion, performing guided filtering noise reduction processing on the original visible light image and the near-infrared fluorescence image acquired by a camera, and inputting the processed images into a trained model network, and a visible light image (the fluorescence is pseudo-color) fused with the near-infrared fluorescence image is obtained. According to the invention, the precision and speed of near-infrared fluorescence imaging are obviously improved based on the deep learning method.

The present invention discloses a fluorescent probe for detecting O2 ·‑ , which is constructed with silicon rhodamine as the fluorescent group and diphenylphosphinyl as the recognition group. In the presence of O2 ·‑ , O2 ·‑ will perform a nucleophilic attack on the phosphate ester, followed by 1,6 elimination, resulting in the decomposition of the quaternary ammonium salt, releasing the silicon rhodamine luminophore and promoting its ring-opening. Before the reaction, the silicon rhodamine quaternary ammonium salt 8 with a spiro structure remains non-fluorescent and has a low background; after reacting with O2 ·‑ , the free and ring-opened silicon rhodamine dye can be excited to emit near-infrared fluorescence, thereby achieving the purpose of detecting O2 ·‑ , and it will have good application prospects in the field of bio-related sciences. The fluorescent probe for detecting O2 * - in the present invention has the structure shown in Formula 8: #imgabs0#

The invention belongs to the technical field of fluorescent probes and biological detection, and particularly relates to a hypochlorous acid response type near-infrared fluorescent probe for screening Alzheimer's disease antioxidants as well as a preparation method and application of the hypochlorous acid response type near-infrared fluorescent probe. The near-infrared fluorescent probe FP-MT-HClO provided by the invention is subjected to a specific reaction under the action of HClO, generates obvious fluorescence signal enhancement, outputs near-infrared fluorescence signals at about 665 nm, and has the characteristics of quick response, high sensitivity, good selectivity, strong anti-interference capability and the like. An in-vitro screening model used for simulating an inflammatory oxidative stress link related to the Alzheimer's disease is constructed based on the probe, and rapid screening and activity evaluation of candidate antioxidants capable of reducing the HClO level in cells are achieved. The method disclosed by the invention is good in repeatability, is suitable for in-vitro screening of candidates related to inflammatory oxidative stress, and can be used for early evaluation and mechanism research of an oxidative stress process related to Alzheimer's disease.