453 results about "Infrared fluorescence" patented technology

The invention discloses a thyroid intraoperative real-time navigation method and a thyroid intraoperative real-time navigation system based on multi-mode optical fusion. The thyroid intraoperative real-time navigation method comprises the following steps: outputting visible light through an endoscope and coupling light waves of a narrow-band multispectral light source to irradiate an operative field, exciting parathyroid glands to generate near-infrared fluorescence, and receiving reflected visible light, split light, near-infrared fluorescence and laser speckle signals through an endoscope probe. And separating the composite optical signal into four channels, and respectively generating an anatomical structure color image, a blood vessel spectroscopic image, a parathyroid gland near-infrared fluorescence image and a laser speckle image. Performing decorrelation processing on the laser speckle image to generate a blood flow dynamic pseudo-color decorrelation speckle image; an anatomical structure, a blood vessel center line, a parathyroid gland contour and blood flow dynamic feature points are extracted through a multi-modal registration technology, after affine transformation space alignment is conducted, a comprehensive imaging map containing the anatomical structure, blood vessel distribution and parathyroid gland function marking information is generated through a wavelet fusion algorithm, and intraoperative multi-dimensional real-time tissue navigation is achieved.

The invention provides a fluorescence-magnetic particle image fusion method which can be applied to the technical field of medical image processing. The method comprises the following steps: performing two-dimensional convolution processing on a registered two-dimensional near-infrared fluorescence image to obtain a multi-channel near-infrared fluorescence extended feature map, and performing initial fusion on the multi-channel near-infrared fluorescence extended feature map and a registered three-dimensional magnetic particle cross-sectional image to obtain a three-dimensional near-infrared fluorescence extended feature map; performing multi-round three-dimensional convolution processing on the three-dimensional near-infrared fluorescence extended feature map to obtain a multi-scale near-infrared fluorescence feature map, and performing multi-round three-dimensional convolution processing on the registered three-dimensional magnetic particle cross-sectional image to obtain a multi-scale magnetic particle feature map; and based on an adaptive cross attention mechanism, performing multi-round feature map fusion on the multi-scale near-infrared fluorescence feature map and the multi-scale magnetic particle feature map on the same scale, and performing filtering convolution processing on a multi-round feature map fusion result to obtain a three-dimensional fluorescence-magnetic particle fusion image.

The invention relates to a microscope system, in particular to a confocal microscope system of a short-wave infrared band. The system comprises a bright / dark field microscopy subsystem, a photon emission microscopy subsystem, a short wave infrared laser scanning confocal microscopy subsystem, a fluorescence confocal microscopy subsystem and a control module. According to the system provided by the invention, the short-wave infrared fluorescence lifetime curve can be obtained through the electro-luminescence emission and / or photoluminescence emission of the semiconductor device in the integrated circuit, so that the defect type of the semiconductor can be judged. Besides, the system adopts a multi-subsystem optical path multiplexing structure in structural design, short-wave infrared fluorescence generated by exciting a defect or a failure point is collected while observation is facilitated, high integration level is achieved, and observation, positioning and failure analysis of the defect or the failure point can be achieved only by using a single system provided by the invention.

The invention discloses an ultra-wide-spectrum endoscopic imaging device capable of being used for short-wave infrared fluorescence imaging, which is used for in-vivo minimally invasive imaging and comprises an illumination module, a wide-spectrum image transmission module, an imaging module and an image processing module. The illumination module consists of a light source and a light guide optical fiber, and is used for generating illumination light from ultraviolet to short wave infrared band and transmitting the illumination light to the wide-spectrum image transmission module; the wide-spectrum image transmission module consists of a few-interface optical imaging channel and a light guide illumination channel, and is used for illuminating an imaging target, exciting target fluorescence and collecting image signals from ultraviolet to short-wave infrared bands; the imaging module is composed of an optical filter and an imaging camera, and the imaging camera can be a wide spectral response image sensor or can be formed by combining cameras working in different wavebands and is used for converting optical images in different wavebands into image data; and the image processing module is used for registering and fusing image data of different wavebands in real time and displaying the image data.

The invention provides a composition containing folic acid compounds and a near-infrared fluorescence capture system. The near-infrared fluorescence capture system comprises an imaging module, and the imaging module comprises a compound shown in the formula I or pharmaceutically acceptable salt of the compound. The compound shown in the formula I or the pharmaceutically acceptable salt of the compound can be used as a near-infrared fluorescence imaging agent, and is good in targeting property and clinical application prospect. # imgabs0 #

The invention provides a visible light and short wave infrared fluorescent endoscopic imaging system and method, and belongs to the technical field of fluorescent endoscopic imaging, and the system comprises an endoscope which is used for guiding a light signal into a position needing to be checked and collecting a light signal reflected or transmitted from a target tissue; a light source; the light source adopts a multimode light source and is connected with the endoscope through an optical fiber; a camera module; the camera module is connected with the endoscope through the optical bayonet, and the camera module can receive optical signals output by the endoscope and convert the optical signals into image information; and the controller is in control connection with the light source, the camera module and the display, the camera module transmits the image information into the controller for processing, and the display displays the image information processed by the controller. By fusing the visible light imaging mode and the short wave infrared fluorescence imaging mode, surface details and deep structure information of the tissue can be captured at the same time, and the high-resolution and high-definition imaging effect is achieved.

The present disclosure is a polyethylene glycol modified zwitterionic fluorescent probe. The core luminescent group of the fluorescent probe of the present disclosure is a heptamethine chain which is connected by a phenoxy bridge and has a rigid ring structure in the center. The two ends of the heptamethine chain are two sulfonic indole groups, and two polyethylene glycol chains are connected with the indole groups. A tumor targeting ligand is connected with a luminescent group through one polyethylene glycol chain and one alkaline amino acid spacer. The probe of the present disclosure can be connected with polypeptides, small molecules and antibodies having a tumor targeting function, thereby achieving near-infrared fluorescence imaging for tumor targeting. The probe of the present disclosure has good druggability, and is extremely suitable for industrial production and clinical promotion.

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention relates to the technical field of medical chemistry, in particular to a bridge oxygen site regulatively modified rhodamine anti-cancer fluorescent compound as well as a preparation method and application thereof. The invention develops the rhodamine anti-cancer fluorescent compound of which the meso site is functionalized by tetraarylethene and the bridge oxygen site can be regulated and modified, the fluorescent compound has adjustable red light-near infrared fluorescence emission and excellent fluorescence quantum yield, and the quantum yield can be as high as 99%; the fluorescent compound specifically targets mitochondria, and mainly inhibits growth of tumor cells by inducing para-apoptosis; under irradiation of 655 nm near-infrared laser, ferroptosis is further triggered, efficient treatment of tumor cells is achieved through chemical-photodynamic synergistic effect, and a new treatment thought is provided for overcoming tumor drug resistance.

The invention discloses a near-infrared fluorescence sensor, a near-infrared fluorescence sensor array and a preparation method and application of the near-infrared fluorescence sensor array, and belongs to the technical field of biological medicine. The composite material comprises a single-walled carbon nanotube, a single-stranded DNA (deoxyribonucleic acid) and hydrogel, the single-stranded DNA is connected and modified on the surface of the single-walled carbon nanotube, and the single-walled carbon nanotube modified with the single-stranded DNA is fixed in the hydrogel. Bacterial metabolites enter the hydrogel and influence the structure of the single-stranded DNA, so that the fluorescence intensity of the single-walled carbon nanotube is influenced. A plurality of near-infrared fluorescence sensors form an array to quickly capture component change caused by growth of bacteria in an object to be detected, and respond to fluorescence signal change, so as to visualize whether the components change or not. Whether bacteria grow or not is judged through response information of the multiple sensors, so that the bacteria are rapidly recognized, and the method has the advantages of being high in sensitivity, short in response time and small in needed sample size.

The invention discloses a Janus air filtering membrane for capturing, detecting and sterilizing integrated biological aerosol and a preparation method of the Janus air filtering membrane, and belongs to the technical field of filtering membranes. According to the technical scheme, the air filtering membrane is provided with an asymmetric two-side structure and a soluble micro-nano porous network structure, the surface of the filtering membrane is asymmetrically modified through an amphiphilic AIE photosensitizer, the Janus structure is constructed on the basis that the overall structure of the filtering membrane is not damaged, aggregation of the AIE photosensitizer on the single side of the filtering membrane is achieved, and therefore the air filtering membrane is formed. The air filter membrane has green capture detection performance and AIE photodynamic disinfection performance. The preparation method comprises the steps of solution preparation, filter membrane forming, surface asymmetric modification and drying. According to the Janus air filtering membrane, efficient capture and enrichment of biological aerosol can be achieved, the Janus air filtering membrane is used for microbiological detection under the guidance of near-infrared fluorescence imaging after being colored, and effective inactivation of air pathogens can be achieved by means of white light irradiation.

The invention belongs to the technical field of analysis and detection, and particularly relates to a beta-galactosidase response type hemicyanine near-infrared fluorescent probe, a preparation method thereof and application of the beta-galactosidase response type hemicyanine near-infrared fluorescent probe as a tumor organ imaging probe. The near-infrared fluorescent probe provided by the invention takes a water-soluble hemicyanine skeleton as a fluorophore, so that the biocompatibility of the near-infrared fluorescent probe is improved; based on the principle that beta-galactose can be selectively cut by beta-galactosidase, a galactose structure with a self-leaving group is used as a response group, selective quick response to the beta-galactosidase is achieved, and high sensitivity to the beta-galactosidase is shown. The near-infrared fluorescent probe provided by the invention can emit near-infrared fluorescence with long wavelength, has lower background fluorescence and good penetration depth, and can be directly used for detecting organoids containing the matrigel without eliminating the matrigel, so that the organoids are prevented from being damaged or additional experimental variables are prevented from being introduced in the process of eliminating the matrigel.

The present invention relates to labeled compounds suitable for positron emission tomography (PET) imaging and / or fluorescence imaging such as near-infrared fluorescence imaging (NIRF). The invention further relates to the use of these compounds for carrying out PET scans. imaging calcium sensing receptor (CSR)-positive organs. excising parathyroid tissue. protecting parathyroid tissue during thyroid surgery, and treating disorder(s) of a CSR-positive tissue in a subject.

The invention belongs to the technical field of antitumor drugs, and particularly discloses a preparation method and application of a compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment. The compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment is shown in the following structural formula: # imgabs0, the molecular skeleton of the compound contains a triphenylamine unit and an N, N-dimethylaniline fragment, intramolecular rotation limitation and intramolecular vibration limitation can be generated, and obvious aggregation-induced emission (AIE) performance is shown. The function-integrated phototherapy agent has high active oxygen generation efficiency, photothermal conversion efficiency and near-infrared fluorescence quantum yield, and is beneficial to obtaining ideal photothermal-photodynamic therapy effect for deep tumors. The phototherapy agent is novel in structure, the preparation method is easy in raw material obtaining, the synthesis condition is mild, the preparation method is simple, purification is convenient and fast, implementation is easy, and the phototherapy agent has great application prospects.

The invention discloses construction of a near-infrared fluorescent carbon dot based on metformin. The carbon dot is synthesized from metformin and an amino acid compound through a one-pot hydrothermal method. The preparation method comprises the following steps: mixing the metformin and the amino acid compound, dissolving in an alkaline solution, carrying out ultrasonic dissolution, transferring the solution into a polytetrafluoroethylene inner container, putting the inner container into a stainless steel high-pressure kettle, and carrying out heating reaction in a muffle furnace. After the reaction is finished and a reaction system is cooled to room temperature, taking out the solution and centrifuging to obtain supernate; and then putting the supernate into a dialysis bag for dialysis purification, and after dialysis is finished, freeze-drying the solution to obtain the carbon dots MACDs disclosed by the invention. The prepared carbon dot has excellent biocompatibility and optical property adjustability, retains the characteristic structure of metformin, has near-infrared fluorescence emission, and can realize disease diagnosis of organisms.

The invention belongs to the technical field of biological medicine, and relates to a small-molecule near-infrared fluorescent probe SHP-PP and a preparation method and application thereof.The small-molecule near-infrared fluorescent probe SHP-PP is composed of a targeted SHP2 recognition group, a linking group and a fluorophore Py5, keeps high affinity to SHP2, can specifically mark SHP2 in living cells, and can be used for detecting the SHP2 in the living cells. According to the present invention, the probe has characteristics of high sensitivity, high protein selectivity, maximum reduction of interference of other proteins, excellent optical property, imaging requirement meeting, maximum emission wavelength in the near-infrared region, large Stokes displacement reaching 200 nm, high signal-to-noise ratio, stable fluorescence signal, and low phototoxicity, and can be used for the preparation of the fluorescent probe, such that the probe has characteristics of high signal-to-noise ratio, stable fluorescence signal, and low phototoxicity.

The invention discloses a boron difluoride curcumin skeleton compound as shown in a formula (I), and belongs to the field of medical imaging molecular probes. The boron difluoride curcumin skeleton compound disclosed by the invention has the characteristics of near-infrared emission and large Stokes shift, can be used as a fluorescent probe for marking key pathological proteins such as beta-amyloid protein (Abeta) aggregate of Alzheimer's disease (AD) and the like, and has the characteristics of high sensitivity, high signal-to-noise ratio and high imaging; in addition, in-vivo level A beta imaging can be realized through a blood brain barrier. The boron difluoride curcumin skeleton compound disclosed by the invention also has a treatment effect and can be used for remarkably inhibiting A beta monomers from forming aggregates and depolymerizing A beta aggregates, and meanwhile, the compound shown as the formula (I) is used for remarkably inhibiting A beta-induced microglial cell inflammatory response. The invention also provides a preparation method of the boron difluoride curcumin skeleton compound, an intermediate compound and application of the boron difluoride curcumin skeleton compound. # imgabs0 #

The invention discloses an ultra-sensitive viscosity fluorescent probe based on a carbon-nitrogen double bond. The chemical structural formula of the probe is as follows: # imgabs0 #. The fluorescent probe is synthesized through Schiff base reaction, and is simple to operate and easy to synthesize. The probe can freely rotate at carbon-carbon double bonds and carbon-nitrogen double bonds, and when the probe is in high viscosity, free rotation of molecules is limited, so that twisted intramolecular charge transfer is inhibited, fluorescence of the probe is remarkably enhanced, and 650 nm near-infrared fluorescence is emitted under the excitation wavelength of 600 nm. In addition, the probe has ultrahigh sensitivity, excellent selectivity, excellent light stability and pH tolerance, and has huge application prospects in the technical fields of analytical chemistry, life science, biomedical treatment and the like.

The invention provides a high-luminous-efficiency full-spectrum light source manufacturing method and an LED device. The method comprises the steps that a metal support is provided; a light-emitting chip is welded to the metal support so that the whole metal support can form a channel, and the wave band range of the light-emitting chip is 400 nm to 480 nm; infrared fluorescent powder and glue are evenly mixed, bubbles are removed, infrared fluorescent powder packaging glue is obtained, the infrared fluorescent powder packaging glue is dispensed above the light-emitting chip, first fluorescent glue is formed, and the waveband range of the infrared fluorescent powder is 680-780 nm; mixing white light combined fluorescent powder with glue to obtain white light combined packaging glue, coating the white light combined packaging glue on the first fluorescent glue, and drying the white light combined packaging glue to obtain the full-spectrum light source with high luminous efficiency.

The invention discloses a near-infrared confocal microscopic imaging system and a near-infrared confocal microscopic imaging method, and relates to the technical field of optical microscopic imaging. The method comprises the following steps that near-infrared exciting light sequentially passes through a beam expanding system, a liquid lens and a confocal scanning light path to irradiate a to-be-detected sample so as to generate a near-infrared fluorescence signal, and the liquid lens is used for adaptively adjusting the focal length and wavefront morphology according to the depth of the to-be-detected sample; a photomultiplier detects the near-infrared fluorescence signal after non-focus scattered light is filtered out through the confocal scanning light path and the pinhole, and a fluorescence signal image is obtained; and according to the fluorescence signal image, carrying out iterative optimization on the focal length and phase modulation parameters of the liquid lens to maximize the image sharpness or the signal-to-noise ratio and realize the three-dimensional microscopic imaging of the deep tissue. According to the near-infrared confocal microscopic imaging method provided by the invention, deep near-infrared microscopic imaging with low phototoxicity, low cost and high resolution can be realized.

The invention provides an anoxic and endogenous formaldehyde dual-response supramolecular fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of fluorescent probes. The supramolecular fluorescent probe provided by the invention is composed of azobenzene calixarene and a formaldehyde response fluorescent probe included in the azobenzene calixarene. The supramolecular fluorescent probe provided by the invention can simultaneously respond to oxygen deficit and high endogenous formaldehyde microenvironment characteristics and generate a stronger near-infrared fluorescent signal for analysis and detection, so that false positive signal interference is reduced, high-specificity imaging under tumor microenvironment conditions can be realized, and a new mode is provided for precise imaging of tumors. Compared with the existing fluorescent probe, the supramolecular fluorescent probe provided by the invention combines the detection of two important biomarkers in the tumor generation and development processes, and does not need to introduce a large-volume targeting group into the molecule; the preparation method is simple, the structure is novel, the imaging performance is excellent, and the tumor is accurately subjected to near-infrared fluorescence imaging tracing.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

The invention discloses lead-free halogen double perovskite near-infrared fluorescent powder and a preparation method thereof, and the chemical general formula of the fluorescent powder is Cs2M1-x-yCl6: xPt < 4 + >, yEr < 3 + >, wherein M is any one of Zr, Sn and Hf, and 0 lt; x is less than or equal to 0.02, 0lt; y < = 0.35. The preparation method comprises the following steps: adding an erbium acetate solution into a platinum tetrachloride solution, and sealing and stirring; adding the obtained solution into an MCl4 solution, and sealing and stirring at 50-70 DEG C; adding a cesium chloride solution, sealing and stirring at 40-60 DEG C, and after the solution is cooled to room temperature, carrying out centrifugal separation and centrifugal washing; and drying at 40-60 DEG C, and cooling. The emission peak of the obtained fluorescent powder Pt < 4 + > well covers the excitation peak of Er < 3 + >, energy is effectively transmitted to a light-emitting center Er < 3 + >, and the light-emitting efficiency of Er < 3 + > is remarkably improved; strong absorption is realized in ultraviolet, near ultraviolet and blue light areas.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The utility model discloses an imaging system. The imaging system comprises a light source, an endoscope connected with the light source, a second dichroscope, a visible light camera and a near-infrared camera. The light source comprises an excitation light source, an illumination light source and a first dichroscope used for combining light beams emitted by the excitation light source and the illumination light source. A light beam emitted by the light source is irradiated to a target object through the endoscope, so that the target object reflects visible light and is excited to emit near-infrared fluorescence, and the endoscope is used for collecting the visible light and the near-infrared fluorescence from the target object; the second dichroscope is arranged on the optical axis of the endoscope and is used for dividing the light from the endoscope into visible light and near-infrared fluorescence; and the visible light camera and the near-infrared camera are respectively used for imaging the visible light and the near-infrared fluorescence from the second dichroscope. In this way, the light beams emitted by the excitation light source and the illumination light source can irradiate the same position of the target object, and imaging of near-infrared fluorescence and visible light of the same scene is achieved.

The present invention relates to a fluorescent compound based on new indocyanine green IR820 and its preparation and application. The molecular structure of the fluorescent compound is shown in formula (1): Compared with the prior art, the fluorescent probe of the present invention has near-infrared fluorescence emission, good tumor targeting, biocompatibility, low cytotoxicity, and strong biological tissue penetration. It can be used for targeted fluorescence imaging of tumor cells and living tumors, and is expected to be further used in fluorescence surgical navigation.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The invention discloses a difunctional near-infrared fluorescent probe as well as a synthesis method and application thereof. The structural formula of the fluorescent probe is shown as a formula (I). The fluorescent probe DTPD provided by the invention can be used for fluorescent quantitative detection of hypochlorous acid and viscosity, and the fluorescent probe can realize detection of hypochlorous acid with high selectivity in an aqueous solution and is not interfered by other coexisting substances. Besides, the near-infrared fluorescent probe provided by the invention has relatively strong organism penetrability in a near-infrared region, can also reduce the interference of autofluorescence of organisms, and has a good cell imaging effect. And # imgabs0 #.

The structural formula of the oxygen-rhodamine near-infrared fluorescent dye is shown as a formula (a) or a formula (b), and R1 and R2 are respectively selected from hydrogen, alkyl or alkenyl; r3 and R4 are respectively selected from hydrogen, alkyl and alkenyl; r5-R9 are respectively selected from hydrogen, alkyl, methoxyl, ethyoxyl, halogen, carboxyl, sulfonic acid group, ethylene glycol group, hydroxyl or amino, and N is an integer greater than or equal to 0. The invention also discloses a preparation method and application of the fluorescent dye. The compound is novel in structure, excellent in performance, simple in preparation method and good in product application prospect.

The present invention provides a large Stokes shift near-infrared fluorescent probe for detecting HClO, its preparation method and biological imaging application, belonging to the field of bioanalysis technology. Based on the strategy of modifying the substituents at the 3,6 positions of xanthene, the present invention constructs a large Stokes shift near-infrared fluorescent probe for specifically recognizing HClO. Optical experiments show that the emission wavelength of the probe for detecting HClO is 776 nm, the Stokes shift reaches 148 nm, with strong selectivity, high sensitivity, and a detection limit as low as 4.95 nM, which can greatly reduce the interference of background fluorescence in biological imaging applications. The fluorescent probe of the present invention has almost no cytotoxicity and can be applied to the detection and imaging of HClO in living cells MCF-7 and rat liver tissues, with a tissue penetration depth of 140 μm.