284 results about "Infrared fluorescence" patented technology

The invention discloses an ultra-wide-spectrum endoscopic imaging device capable of being used for short-wave infrared fluorescence imaging, which is used for in-vivo minimally invasive imaging and comprises an illumination module, a wide-spectrum image transmission module, an imaging module and an image processing module. The illumination module consists of a light source and a light guide optical fiber, and is used for generating illumination light from ultraviolet to short wave infrared band and transmitting the illumination light to the wide-spectrum image transmission module; the wide-spectrum image transmission module consists of a few-interface optical imaging channel and a light guide illumination channel, and is used for illuminating an imaging target, exciting target fluorescence and collecting image signals from ultraviolet to short-wave infrared bands; the imaging module is composed of an optical filter and an imaging camera, and the imaging camera can be a wide spectral response image sensor or can be formed by combining cameras working in different wavebands and is used for converting optical images in different wavebands into image data; and the image processing module is used for registering and fusing image data of different wavebands in real time and displaying the image data.

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention relates to the technical field of medical chemistry, in particular to a bridge oxygen site regulatively modified rhodamine anti-cancer fluorescent compound as well as a preparation method and application thereof. The invention develops the rhodamine anti-cancer fluorescent compound of which the meso site is functionalized by tetraarylethene and the bridge oxygen site can be regulated and modified, the fluorescent compound has adjustable red light-near infrared fluorescence emission and excellent fluorescence quantum yield, and the quantum yield can be as high as 99%; the fluorescent compound specifically targets mitochondria, and mainly inhibits growth of tumor cells by inducing para-apoptosis; under irradiation of 655 nm near-infrared laser, ferroptosis is further triggered, efficient treatment of tumor cells is achieved through chemical-photodynamic synergistic effect, and a new treatment thought is provided for overcoming tumor drug resistance.

The invention discloses a near-infrared confocal microscopic imaging system and a near-infrared confocal microscopic imaging method, and relates to the technical field of optical microscopic imaging. The method comprises the following steps that near-infrared exciting light sequentially passes through a beam expanding system, a liquid lens and a confocal scanning light path to irradiate a to-be-detected sample so as to generate a near-infrared fluorescence signal, and the liquid lens is used for adaptively adjusting the focal length and wavefront morphology according to the depth of the to-be-detected sample; a photomultiplier detects the near-infrared fluorescence signal after non-focus scattered light is filtered out through the confocal scanning light path and the pinhole, and a fluorescence signal image is obtained; and according to the fluorescence signal image, carrying out iterative optimization on the focal length and phase modulation parameters of the liquid lens to maximize the image sharpness or the signal-to-noise ratio and realize the three-dimensional microscopic imaging of the deep tissue. According to the near-infrared confocal microscopic imaging method provided by the invention, deep near-infrared microscopic imaging with low phototoxicity, low cost and high resolution can be realized.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The present invention relates to a fluorescent compound based on new indocyanine green IR820 and its preparation and application. The molecular structure of the fluorescent compound is shown in formula (1): Compared with the prior art, the fluorescent probe of the present invention has near-infrared fluorescence emission, good tumor targeting, biocompatibility, low cytotoxicity, and strong biological tissue penetration. It can be used for targeted fluorescence imaging of tumor cells and living tumors, and is expected to be further used in fluorescence surgical navigation.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The structural formula of the oxygen-rhodamine near-infrared fluorescent dye is shown as a formula (a) or a formula (b), and R1 and R2 are respectively selected from hydrogen, alkyl or alkenyl; r3 and R4 are respectively selected from hydrogen, alkyl and alkenyl; r5-R9 are respectively selected from hydrogen, alkyl, methoxyl, ethyoxyl, halogen, carboxyl, sulfonic acid group, ethylene glycol group, hydroxyl or amino, and N is an integer greater than or equal to 0. The invention also discloses a preparation method and application of the fluorescent dye. The compound is novel in structure, excellent in performance, simple in preparation method and good in product application prospect.

The application discloses a near-infrared fluorescent powder and a light-emitting device. The near-infrared fluorescent powder comprises a composition formula LiA a Sc 1‑a‑p‑d‑ c D d P p An inorganic compound of EO4:cCr, wherein A elements include one or two of Li, Na and K, D elements include two of Ga, In, Al, Ce and Bi, and E elements include two or three of Hf, Ge and Si; wherein 0

This invention discloses a method for preparing HVC NPs for the diagnosis and treatment of Parkinson's disease and their applications. The probe HV in the HVC NPs responds to H2O2 and viscosity, utilizing near-infrared fluorescence imaging capabilities to achieve real-time monitoring of Parkinson's disease biomarkers, helping us better understand the occurrence and development of Parkinson's disease. With the assistance of RVG29, the HVC NPs effectively cross the blood-brain barrier. The curcumin within them can reduce cell apoptosis, lower oxidative stress levels, scavenge excess reactive oxygen species, combat inflammation, and reduce α-synuclein aggregation, demonstrating effective anti-Parkinson's disease effects at the cellular and experimental animal levels. This nanomaterial provides a new strategy for integrated research on the diagnosis and treatment of Parkinson's disease.

The invention discloses a near-infrared fluorescence imaging agent of a targeted folate receptor, an imaging system and application of the imaging agent and the imaging system, and belongs to the technical field of biomedical treatment. The technical problem to be solved is to provide the folate receptor-targeted near-infrared fluorescence imaging system, an imaging agent in the system can specifically target the folate receptor FR-alpha, and the folate receptor-targeted near-infrared fluorescence imaging system is used in application scenes of lung cancer and ovarian cancer and can show higher TNR, display tumor boundaries in real time and reduce the positive edge cutting rate. According to the technical scheme, the near-infrared fluorescence imaging agent of the targeted folic acid receptor is characterized by comprising a compound of the targeted folic acid receptor, a solvent and pharmaceutically acceptable auxiliary materials, the structure of the compound of the targeted folic acid receptor is F-L-D, F is the structure of the targeted folic acid receptor, D is a near-infrared dye, L is a connexon, and L is an electron donor. The structural formula is shown in the specification or pharmaceutically acceptable salts thereof.

The present invention discloses an LED light source for full-spectrum plant lighting, comprising a multi-channel bracket, an ultraviolet chip, a first blue light chip, a second blue light chip, a red light chip, a red light fluorescent glue layer, a near-infrared fluorescent glue layer, a first fluorescent glue layer, a second fluorescent glue layer and a multi-channel driver, wherein: the ultraviolet chip, the first blue light chip, the second blue light chip and the red light chip are arranged in series or in parallel on the upper surface of the bracket; the upper surface of the ultraviolet chip is coated with a red light fluorescent glue layer and a near-infrared fluorescent glue layer in sequence; the first fluorescent glue layer is dotted above the first blue light chip; the second fluorescent glue layer is dotted above the second blue light chip; the bracket is filled with transparent silica gel, and the multi-channel driver electrically connects and controls the current of the ultraviolet chip, the first blue light chip, the second blue light chip and the red light chip respectively.

The invention belongs to the field of molecular fluorescent probes, and particularly discloses a near-infrared fluorescent probe of quinone oxidoreductase 1 (NAD (P) H: quinone oxidoreductase 1, NQO1) with an in-situ covalent anchoring function and a preparation method of the near-infrared fluorescent probe. According to the probe, dicyanoisophorone is used as a core fluorophore, an aldehyde group is used as an NQO1 covalent anchoring group, and 3-methyl-3-(2, 4, 5-trimethyl-3, 6-dioxocyclohex-1, 4-diene-1-yl) butyrate is used as a recognition group of NQO1. The invention also provides an application of the near-infrared fluorescent probe with the in-situ covalent anchoring NQO1. Compared with other probes, the probe is simple in synthesis method and suitable for industrial production, and has high sensitivity, high selectivity and good biocompatibility in NQO1 detection. The fluorescent probe disclosed by the invention can realize covalent labeling and in-situ imaging functions after target NQO1 recognition response. Long-time in-situ tracking and imaging can be carried out on tumor cells and tissues by monitoring the NQO1 level, and a simple and effective method is provided for accurate detection of tumor disease related protease.

The present application relates to the technical field of nanoprobes, and particularly relates to a near-infrared fluorescence / magnetic resonance dual-mode probe and a preparation method and application thereof. The present application adopts a Brij98 amphiphilic molecule to wrap an IR824Et-PhDTA-Gd near-infrared fluorescence / magnetic resonance dual-response molecule to prepare the probe, so that the probe has an absorption wavelength of 650-850 nm, generates a fluorescence signal under excitation of 785 nm laser, and the generated fluorescence wavelength can reach 1000 nm, which is in a near-infrared region (780-1700 nm); the probe has good positive magnetic resonance response characteristics, and a longitudinal relaxation rate (r1) of the probe is 28 mM ‑1 s ‑1 , which is 7 times of that of a clinically used Magnevist; the probe can be applied in the fields of fluorescence and / or magnetic resonance imaging, and has a wide application prospect. The preparation method of the near-infrared fluorescence / magnetic resonance dual-mode probe is simple in operation and low in cost.

The invention relates to the technical field of biological detection, in particular to an HClO near-infrared fluorescent probe as well as a preparation method and application thereof. The structure of the HClO near-infrared fluorescent probe is shown in the specification. The emission wavelength of the near-infrared fluorescent probe developed by the invention is 750nm, and the near-infrared fluorescent probe has high selectivity, high sensitivity (the detection limit is as low as 49 nM) and quick response capability to HClO, and has low cytotoxicity and excellent in-vivo imaging capability, so that visual observation of HClO in a living Parkinson's disease model mouse body is successfully realized, and the near-infrared fluorescent probe has a wide application prospect. The method has great potential in the aspect of tracking HClO related pathological states in real time.

The invention belongs to the field of biomedical molecular images, particularly relates to an endoscope visible light image and near-infrared fluorescence image fusion method based on unsupervised deep learning, and aims to overcome the defects that splicing fusion of visible light images and near-infrared fluorescence images is inaccurate and low in speed and the like. The method comprises the following steps: acquiring an original visible light image and an original near-infrared fluorescence image as a training set of data, constructing and training a convolutional neural network based on unsupervised image fusion, performing guided filtering noise reduction processing on the original visible light image and the near-infrared fluorescence image acquired by a camera, and inputting the processed images into a trained model network, and a visible light image (the fluorescence is pseudo-color) fused with the near-infrared fluorescence image is obtained. According to the invention, the precision and speed of near-infrared fluorescence imaging are obviously improved based on the deep learning method.

The invention belongs to the technical field of fluorescent probes and biological detection, and particularly relates to a hypochlorous acid response type near-infrared fluorescent probe for screening Alzheimer's disease antioxidants as well as a preparation method and application of the hypochlorous acid response type near-infrared fluorescent probe. The near-infrared fluorescent probe FP-MT-HClO provided by the invention is subjected to a specific reaction under the action of HClO, generates obvious fluorescence signal enhancement, outputs near-infrared fluorescence signals at about 665 nm, and has the characteristics of quick response, high sensitivity, good selectivity, strong anti-interference capability and the like. An in-vitro screening model used for simulating an inflammatory oxidative stress link related to the Alzheimer's disease is constructed based on the probe, and rapid screening and activity evaluation of candidate antioxidants capable of reducing the HClO level in cells are achieved. The method disclosed by the invention is good in repeatability, is suitable for in-vitro screening of candidates related to inflammatory oxidative stress, and can be used for early evaluation and mechanism research of an oxidative stress process related to Alzheimer's disease.

The invention relates to the technical field of biological medicine, in particular to a TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof, and discloses a fluorescent molecular probe named as' TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof '. The invention aims to solve the problems of large molecular weight, poor pharmacokinetic performance, limited tissue penetration, high imaging background and poor imaging timeliness of the existing antibody fluorescent probe. The probe comprises a TREM-1 targeting module (TDM), a near-infrared fluorescence reporter group, a dark quenching group and a conformational sensitive connecting arm (CSL). The fluorescence of the probe is quenched before the probe is combined with TREM-1, and after the probe is combined with a target spot, TDM specific binding induces CSL conformation rearrangement, so that a reporter group and a quenching group are spatially separated, fluorescence resonance energy transfer (FRET) is relieved, and fluorescence activation is realized. According to the invention, rapid, high-contrast and low-background fluorescence imaging of atherosclerotic plaques is realized, and the probe is small in molecular size, strong in tissue penetrability and rapid in-vivo clearing.

PPAB derived near-infrared super-small fluorescent silica nanoparticles and synthesis method and application thereof, the fluorescent silica nanoparticles prepared by the present application exhibit strong near-infrared fluorescence and good water solubility, have a strong fluorescence emission peak near 650 nm-700 nm wavelength, exhibit good fluorescence spectrum performance, and therefore the silicon quantum dots have relatively good cell tissue penetration. Due to the unique optical properties and relatively good cell tissue penetration, the in vivo fluorescence imaging can be effectively carried out. The fluorescent silica nanoparticles prepared by the present application have excellent singlet oxygen generation performance and peroxidase performance in addition to excellent near-infrared optical performance and good biocompatibility, can be used as a photodynamic / chemodynamic synergistic treatment reagent, and are used for antibacterial and antitumor applications, and have no toxic side effects on the organism itself.

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention discloses an application of a tumor-associated macrophage targeting nanoprobe in ultrasensitive magnetic resonance molecular imaging of in-vivo in-situ lung cancer. The tumor-associated macrophage targeting nanoprobe prepared by the method provided by the invention is loaded with water-insoluble supramolecular celopphane, so that Xe'molecular cage 'celopphane can be uniformly dispersed in water and a biological system, and is used for in-vivo hyperpolarized 129Xe magnetic resonance imaging. According to the nanoprobe, mannose modification is carried out on the surface of the nanoprobe, so that the nanoprobe can effectively target tumor-related macrophages in a tumor area, and targeted detection of in-situ lung cancer tumors is realized; the surface of the nanoprobe is subjected to near-infrared fluorescence molecular modification, so that fluorescence / magnetic resonance bimodal detection of solid tumors can be realized. In combination with the advantage that local drug concentration can be improved by pulmonary drug delivery, a strong 129Xe magnetic resonance signal of the developed probe can be directly detected in vivo, and in-vivo 129Xe magnetic resonance molecular imaging is realized on an in-situ lung cancer model mouse through a direct sampling imaging mode.

The invention discloses a near-infrared photosensitizer for specifically recognizing fibroblast activation protease (FAP-alpha) as well as a preparation method and application of the near-infrared photosensitizer. A cyanine derivative is used as a fluorescent parent nucleus (ICyOH) of the photosensitizer, the fluorescence of the photosensitizer can be almost completely masked by modifying the photosensitizer, and after the photosensitizer reacts with FAP-alpha for 60 minutes, the fluorescence intensity is rapidly enhanced, so that high sensitivity, good selectivity and anti-interference performance are shown. The ZGP-LL-ICy is a photosensitizer with a cyanine structure, FAP-alpha fluorescence enhancement and high selectivity to FAP-alpha, the reagent shows high selective response to FAP-alpha enzyme, active ICyOH molecules with near-infrared fluorescence are released under the action of enzymolysis, and tumor fluorescence imaging of deep tissue penetration is achieved. Meanwhile, the activated ICyOH can generate cytotoxic active oxygen under the illumination condition, and photodynamic therapy on pancreatic cancer is achieved. The reagent has the advantages of low background fluorescence, deep tissue penetration, diagnosis and treatment integration and the like, and has important application value in the fields of early diagnosis and precise treatment of pancreatic cancer.

The invention discloses a tyrosinase near-infrared fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of analytical chemistry. According to the technical scheme, the tyrosinase near-infrared fluorescent probe is characterized in that the structural formula of the tyrosinase near-infrared fluorescent probe is shown in the specification. The invention further specifically discloses a preparation method of the tyrosinase near-infrared fluorescent probe and application of the tyrosinase near-infrared fluorescent probe to selective detection of tyrosinase in a water environment, a biological cell system and living organisms. The near-infrared fluorescent probe disclosed by the invention has the advantages of fluorescence emission in a near-infrared region, good selectivity, high sensitivity and the like.

This invention relates to ONOO ‑ In the field of detection technology, specifically involving an ONOO ‑ Near-infrared fluorescent probe, its preparation method, and its application. This invention constructs a novel fluorescent probe specifically for detecting ONOO. ‑ The ONOO ‑ Near-infrared fluorescent probe for ONOO ‑ It exhibits good reactivity and is capable of specifically imaging endogenous ONOO in living cells or in vivo systems. ‑ Furthermore, it exhibits a large Stokes shift and an emission wavelength of 760 nm, significantly higher than other near-infrared fluorescent probes based on hemicyanine dye structures. In addition, this ONOO... ‑ The near-infrared emission characteristics of near-infrared fluorescent probes enable them to penetrate deeper into tissues and reduce background interference in bioimaging, which is significant for improving imaging sensitivity and accuracy. Based on these characteristics, ONOO... ‑ Near-infrared fluorescent probes can be used for the diagnosis and research of diseases such as atherosclerosis, neurodegenerative diseases, inflammation, and cancer.

The invention discloses a near-infrared fluorescence immunoassay analyzer, and relates to the field of biomedical engineering.The near-infrared fluorescence immunoassay analyzer comprises an analyzer body, a dome-type fluorescence collector is installed in an inner cavity of a sealing seat, a narrow-band light filter is installed at the bottom of the dome-type fluorescence collector, and a piezoelectric focusing lens set is installed at the bottom of a light source sliding block; a light source sliding block is arranged in the analyzer body, a laser range finder and an optical fiber beam splitter are respectively arranged on two sides of the light source sliding block, a double-layer reagent card base body is arranged in the center of the top of the analyzer body, a snakelike microfluidic channel is formed in the top of the double-layer reagent card base body, a shape memory alloy is arranged at the bottom of the double-layer reagent card base body, and a thermosensitive actuator array is arranged at the bottom of the shape memory alloy. According to the near-infrared fluorescence immunoassay analyzer, eight-path parallel scanning is combined with dynamic focusing, so that the single-sample full-index detection time can be greatly shortened, the near-infrared fluorescence immunoassay analyzer can be compatible with various complex biological samples, a chemical color developing agent is not needed, and the experiment cost and the environmental pollution risk are reduced.

The invention relates to a chromium ion single-doped ultra-wideband near-infrared fluorescent material, a preparation method thereof and an LED device, and belongs to the technical field of luminescent materials. The chemical composition formula of the material is Ca2Sc1-yYyGa3-3xSn2O12: xCr, x is larger than or equal to 0.003 and smaller than or equal to 0.10, and y is larger than or equal to 0.00 and smaller than or equal to 1.00. The material is high in phase purity, simple in preparation process, simple and convenient to operate, environment-friendly and high in synthesis efficiency. Under excitation of 475-500 nm, the emission spectrum of the material covers the range of 600-1600 nm, the material has high luminous intensity in a first near-infrared region (about 855 nm) and a second near-infrared region (about 1300 nm), and the full width at half maximum can reach 200-655 nm. Meanwhile, the material has good absorption in the range of 400-700 nm, can be effectively matched with a commercial blue light LED chip, and is suitable for preparing a high-performance near-infrared LED light source. Compared with the prior art, the material provided by the invention not only can realize ultra-wideband emission covering a first near-infrared area and a second near-infrared area, but also is adjustable in emission peak position and high in absorption characteristic and commercial blue light chip compatibility, and is beneficial to development of high-efficiency and high-stability near-infrared LED devices.

The application provides a near-infrared fluorescent pH probe and a preparation method and application thereof, and belongs to the technical field of biosensing; the chemical structural formula of the probe is as follows: the novel near-infrared fluorescent pH probe can detect in a more acidic range, for example, when the pH changes from 6.40 to 3.00, the fluorescence intensity at 790 nm is enhanced by nearly 70 times, and the fluorescent effect is better; the fluorescent pH probe has low cytotoxicity, high stability and good biocompatibility; and can effectively perform fluorescence imaging on different intracellular pH.

The invention belongs to the technical field of biological medicine analysis, and particularly relates to a fluorescent probe sequentially responding to gamma-glutamyltranspeptidase and monoamine oxidase and application of the fluorescent probe. The structure of the fluorescent probe is as shown in a formula 1, the fluorescent probe in the formula 1 is constructed by sequentially covalently connecting gamma-glutamyltranspeptidase and specific recognition groups of monoamine oxidase and integrating the gamma-glutamyltranspeptidase and the specific recognition groups of monoamine oxidase into a benzothiazole-xanthene fluorescent parent structure, and the fluorescent probe has near-infrared fluorescence luminescence performance. The probe adopts a double enzyme response activation strategy, and can be activated and generate a fluorescence signal only when gamma-glutamyltranspeptidase and monoamine oxidase exist at the same time, so that the fluorescence probe is high in specificity and has excellent accuracy and specificity in the aspects of pathological imaging, drug safety evaluation and the like.

The invention discloses an application of a near-infrared fluorescent probe in preparation of a near-infrared first region and near-infrared second region diagnosis, prevention and / or treatment medicine, and belongs to the technical field of organic fluorescent molecules. The near-infrared fluorescent probe specifically targets cMet, has good tumor targeting capability and water solubility, also has near-infrared two-region developing capability, and has huge development potential and application prospect in fluorescence-guided intraoperative navigation.