95results about How to "Enhanced fluorescence emission" patented technology

Nucleotides comprising a reporter moiety and a polymerase enzyme blocking moiety in which the reporter moiety does not also act as a polymerase enzyme blocking moiety are described. Also described are compounds of Formula (I): wherein W is a phosphate group, B is a base, Y is a linker comprising an enzyme-cleavable group, R² is a reporter moiety, R³ is selected from H or OH, Z and Z′ are selected from H, OH, or a group X—R¹, wherein X is a linker comprising an enzyme-cleavable group and R¹ is a polymerase enzyme blocking group, provided that at least one of Z and Z′ is X—R¹.

The present invention relates to an assay including a surface having silver colloids or islands attached thereto. Attached to the surface and/or silver colloids/islands are polynucleotides which are complimentary to a target polynucleotide sequence. The assay is performed by adding the target polynucleotide sequence to the assay surface and allowed to hybridize with the capture polynucleotides. Fluorophore-labeled capture polynucleotides are added and hybridize to the target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence.

A lamp uses a solid state source to pump one or more doped semiconductor nanophosphors to produce a light output of a desired characteristic. The nanophosphor(s) is dispersed in a material, examples of which include liquids and gases. Various nanophosphors are discussed. In the examples, the material with the doped semiconductor nanophosphor(s) dispersed therein appears at least substantially clear when the lamp is off. The exemplary lamp also includes circuitry for driving the solid state source and a housing that at least encloses the drive circuitry. The lamp has a lighting industry standard lamp base mechanically connected to the housing and electrically connected to provide electricity to the circuitry for driving the solid state source.

The present invention relates to novel fluorophores and their use in combination with novel nucleic acid molecules, called aptamers, that bind specifically to the fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described.

The invention provides a preparation method of a fluorescent probe and an application of the fluorescent probe in aluminum ion detection. The fluorescent probe is obtained by virtue of a reaction in a heating reflux manner between 2-hydroxyl-1-naphthaldehyde and carbohydrazide. In the structure of the fluorescent probe provided by the invention, the N atoms on the C=N double bonds are influenced by a photo-induced electron transfer effect, and after the N atoms are combined with aluminum ions, the photo-induced electron transfer effect is inhibited, and planar conjugation is enhanced at the same time; two mechanisms together induce obvious enhancement of fluorescence emission; the fluorescent probe has good selectivity and sensitivity to the aluminum ions. High-selectivity identification of the aluminum ions is realized, a relatively good linear relation exists in a range of 5*10<-6> to 4*10<-5> mol/L, and the limit of detection is as low as 1.89*10<-8> mol/L, so that the fluorescent probe has a chromogenic sensing function.

The present invention relates to a method for screening and identifying compounds that modulate the activity tRNA splicing endonuclease. In particular, the invention provides assays for the identification of compounds that inhibit animalia tRNA splicing endonuclease. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads useful for treating and/or preventing cancer.

The invention discloses a fluorescence probe based on 1, 3, 4-oxadiazole derivative, and a synthetic method and applications thereof. Chemical formula of the fluorescence probe is represented in the invention. The synthetic method comprises following steps: N-[2-(5-phenyl-1, 3, 4-oxadiazole-2-yl)phenyl]-2-chloroacetamide, di-(pyridylmethylene)amine, N, N-diisopropylethylamine and potassium iodide are added into acetonitrile so as to obtain a mixture; the mixture is stirred, and is subjected to heating reflux under protection of nitrogen; solvent removing is realized via reduced pressure distillation, and obtained residues are subjected to column chromatography for separation so as to obtain the fluorescence probe N-[2-(5-phenyl-1, 3, 4-oxadiazole-2-yl)phenyl]-2-di(2-pyridylmethylene) acetamide. The raw materials are easily available; the synthetic method is simple; product separation and purification processes are easy; and the fluorescence probe possesses excellent water solubility, can be used for Zn<2+> monitoring analysis and tracer analysis in water environmental systems and biological cell systems, and possesses excellent sensitivity and relatively strong anti-interference capability on detection of Zn<2+>.

The present invention relates to methods for detecting the presence of ribonuclease enzymes, more specifically to methods that provide for a visual detection assay. The methods entail contacting a test sample suspected of containing ribonuclease activity with a substrate containing a ribonuclease-sensitive internucleotide linkage flanked directly or indirectly by a fluorescence reporter group and a dark quencher, such that if a ribonuclease activity is present in the sample, the ribonuclease-sensitive internucleotide linkage is cleaved and the fluorescence reporter group emits a visually detectable signal. The present invention further provides novel nucleic acid compositions used as substrates for such assays and encompasses kits for performing the methods of the invention.

The present invention discloses size controlled and stabilized nano-aggregates of molecular ultra small clusters of noble metals and a process for the preparation thereof. The present invention discloses single source multicolor noble metal spherical and uniform nano aggregates of 10-22 nm made up of discrete molecular ultra small noble metal (Nb) nanoclusters (MUSNbNC's) of 1-6 atoms. The MUSNbNC's are capped with amine/DCA (dicarboxy acetone) group acting as a steric and kinetic hindrance for core growth suppressing the further autocatalysis and conversion of super critical nucleus or growth by ripening and formation of nanoparticles and thus having intense fluorescence.

Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays. The dyes are of the structure:

in which Z¹ and Z² independently represent a phenyl or a naphthyl ring system; X and Y are selected from oxygen, sulphur, —CH═CH— and the group:

R¹ and R² are selected from C₁-C₄ alkyl, —(CH₂)_n—P, —{(CH₂)₂—O}_p—R⁶ and group W; where P is selected from COOR⁷, SO₃⁻ and OH, W is mono- or di-substituted nitrobenzyl, R⁶ is methyl or ethyl, R⁷ is selected from H, C₁-C₄ alkyl and CH₂OC(O)R⁸, where R⁸ is methyl, or t-butyl, n is an integer from 1 to 10, and p is an integer from 1 to 3; R³ and R⁴ are selected from hydrogen, NO₂, halogen, SO₃⁻, C₁-C₄ alkoxy and —(CH₂)_m, —COOR⁷; where R⁷ is hereinbefore defined and m is 0 or an integer from 1 to 5; R⁵ is C₁-C₆ alkyl optionally substituted with COOR⁷, SO₃⁻, or OH; where R⁷ is hereinbefore defined; and at least one of groups R¹, R², R³ and R⁴ comprises at least one N0₂ group. Also provided are methods for screening for a test agent whose effect upon nitroreductase enzyme activity and nitroreductase gene expression is to be determined.

The invention belongs to the technical field of luminous materials and relates to a nanogold shell coated upconversion nanocrystal composite structure material and a preparation method of the material. The material solves the technical problems of large nano particle size, low fluorescence intensity, incomplete core-shell structure and poor biological tissue penetration capacity of the existing rare-earth upconversion luminescent material. The invention provides the nanogold shell coated upconversion nanocrystal composite structure material in a fluoride and oxide upconversion nano core-shellstructure. The invention further provides the preparation method of the composite structure material. Compared with the upconversion luminescent material prepared by the traditional method, the nanogold shell coated upconversion nanocrystal composite structure material is small in particle size, regular in pattern, good in dispersity, good in biocompatibility, and easy in surface biological functionalization; a gold shell is ultrathin, complete and consecutive; and selectively reinforced fluorescence emission is obtained by adjusting a position of an SPR (surface plasmon resonance) absorptionpeak. The preparation method of the material has the advantages of good controllability, low reaction temperature, low equipment requirements, simple in-situ synthesis operation technology and the like.

The invention provides an erbium ion doped intermediate infrared luminous fluorine tellurate glass. In terms of mol percentage, the glass comprises the following components by mole percent: 43-53 % of ZrF4, 18-22 % of CaF2, 3-5 % of YF3, 2-4 % of AlF3, 18-22 % of NaF, 0-5 % of TeO2, 0-5 % of GeO2, 0-5 % of Bi2O3 and 2-8 % of ErF3. The glass is prepared by using a covered platinum crucible and a silicon carbide rod electric furnace melting method. According to the invention, after heavy metal oxide is introduced, a fluoride glass is still transparent without crystallization, the infrared transmission rate of the glass is 85 % near intermediate infrared 2.7 microns, the heat stability of the glass is improved and the chemical stability of the glass is also improved; under laser diode pumping with wavelength of 980 nm, obviously enhanced intermediate infrared 2.7 microns fluorescence can be obtained; and the erbium ion doped intermediate infrared luminous fluorine tellurate glass provided by the invention is suitable for preparing and using erbium ion doped special glass and optical fiber materials which are luminous at intermediate infrared wave band of 2.7 microns.

The invention discloses a novel near-infrared high-quantum-yield dye which has the following structural formula I shown as the original specification, wherein R1, R2, R6 and R7 are hydrogen, a low-level alkyl, an ether group and a substituted alkyl or acyl that are independent respectively; R3, R4, R5, R8, R9 and R10 are hydrogen, a low-level alkyl or halogen that are independent respectively; R11 is hydrogen, methyl, a cyano group or trifluoromethyl; X<theta> is a negative ion. The invention discloses a preparation method of the dye. The dye has good biocompatibility, low toxicity, longer fluorescence-emission and high fluorescence quantum yield, the autofluorescence of a background is avoided, a fluorescence image with high signal-to-noise ratio can be obtained, and the dye is used for fluorescence labeling for nucleolar RNA in a biological system and plays an important pole in a study process of physiology related to the nucleolar RNA.

The invention discloses a Mg-Yb-Er doped lithium niobate laser crystals and a preparation method thereof, belonging to the field of single crystal growth. The laser crystals grow by Czochralski method, and the initial raw materials in the crystal growth formula comprise MgO, Yb2O3, Er2O3, Li2CO3 and Nb2O5 powder. The preparation method comprises the following steps: firstly preparing raw materials, mixing, and pressing, then sintering the block material, and then putting the sintered material in a lifting furnace to grow, and dissolving materials, sowing, seeding, necking, shouldering, conducting diameter growth, pulling out and annealing in the growth, and finally polarizing the grown crystals. The grown Mg-Yb-Er doped lithium niobate laser crystals disclosed herein have long fluorescent lifetime, large absorption and emission cross section, and are expected to be applied in all solid-state middle-infrared band lasers.

The invention discloses a crystal material, which is characterized in that the chemical formula of the crystal material is CaNdxEryLa(1-x-y)Ga3O7, wherein x is greater than or equal to 0.01 and less than or equal to 0.05, and y is greater than or equal to 0.1 and less than or equal to 0.3; the crystal material belongs to a tetragonal system and a space group shown in the description; the crystal material is formed into a laminated electronegative skeleton structure formed by a GaO4 tetrahedron, and Nd<3+>, Er<3+>, Ca2<+> and La<3+> are distributed among layers and have an unordered crystal structure. An Nd<+> ion is doped in Er<+3> activated CaLaGa3O7 crystal to greatly enhance the adsorption efficiency of the crystal for pump light, the efficient laser output of the -2.7mu m waveband of LD pumping is realized, the fluorescence emission of the crystal in an intermediate infrared waveband is enhanced, the service life and the particle number of 4I13/2 are greatly decreased, a self-final state bottleneck effect is inhibited, high gain is kept by laser media in an oscillation process, and the slope efficiency of the laser output is improved.

The present invention relates to an assay for the determination of a microorganism including a lysing chamber having triangular shaped metallic structures wherein the apexes of two triangles are arranged in alignment and forming a reactive zone for placement of the microorganism and lysing by microwave energy for exposing and isolating a target polynucleotide sequence. The isolated target polynucleotide sequence is introduced to an assay system for contact with polynucleotides which are complimentary to the isolated target polynucleotide sequence. Fluorophore-labeled capture polynucleotides are added for hybridizing to any bound target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence.

Device and methods for the incorporation of Quantum-Dots for spectroscopic analysis into biomedical devices are described. In some examples, the Quantum-Dots act as light emitters, light filters or analyte specific dyes. In some examples, a field of use for the apparatus and methods may include any biomedical device or product that benefits from spectroscopic analysis.