111results about How to "Reduce fluorescence" patented technology

The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.

This invention relates generally to methods and apparatus for utilizing energy, e.g., optical radiation, to treat various dermatological and cosmetic conditions. A handheld dermatological device that facilitates viewing and measuring parameters of a treatment area before, during, and after application of a treatment modality, and methods of use therefor, are disclosed. The device can include a deflective device that can steer radiation to control a target position of the radiation. The device can also include a control device to allow a user to control the radiation through manipulation of the deflective device.

The present invention provides a handheld dermatological device for visualizing a skin treatment region prior to, during, or after therapeutic treatment with therapeutic energy. An apparatus according to the teachings of the invention can include an image capture device and a display device mounted to the apparatus and electrically coupled to the image capture device. The display device is capable of displaying images of the treatment area captured by the image capture device. The apparatus can further include a head capable of transmitting therapeutic energy to a treatment area, which can be precisely aligned by the user to a desired portion of the treatment area through the use of the display device. In some embodiments, the apparatus can include one or more illumination sources for illuminating a skin target region, and shield for shielding the image capture device from direct reflection of the illuminating radiation from a selected skin surface portion.

The present invention relates to novel fluorophores and their use in combination with novel nucleic acid molecules, called aptamers, that bind specifically to the fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described.

The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins and other biomolecules. According to another aspect of the invention, an isoelectric point based separation is enabled in a first dimension, and a size based separation in a second dimension.

The application relates generally to methods useful for the selective amplification of one or more target nucleic acid or fragments thereof, as well as compositions and kits comprising said amplification reaction mixtures. More specifically, the application relates to a composite primer that comprises a 5′ promoter portion and a 3′ target-recognition portion which is complementary to the 3′ end portion of a target polynucleotide sequence; and optionally, a means for identifying the 5′ end portion of the target polynucleotide sequence. The amplification reaction mixture comprises at least one handle-stem-loop structure which comprises a 5′ single-stranded handle comprising the promoter portion and a double-stranded stem comprising at least one pair of self-folding segments hybridized to each other, and optionally, a single-stranded loop comprising the sequence between the pair of self-folding segments.

Methods and compositions involving enzyme-activatable fluorophore polymer imaging agents for photodetection of specific tissues and/or human diseases and disorders are provided. In certain embodiments, the imaging agent comprises a hydrophilic polymer backbone (e.g., poly(L)lysine), a hydrophobic fluorophore, and a hydrophilic solubilizing agent. The solubilizing agent may comprise a quarternary ammonium group (e.g., a 1-methylnicotinic group) to enhance self-quenching of the fluorophore. Various methods for the generation and purification of imaging agents are also provided, including methods involving solid phase probe extraction or ion exchange purification.

The invention relates to synthesis and application of an indole hemicyanine dye, which belongs to the synthesis of a functional dye and the application of the functional dye as a fluorescent dye in the field of fine chemistry industry. Indole quaternary ammonium salts and the corresponding aldehyde are condensed to form compounds with the advantages of large molar extinction coefficient, good light stability, easy synthesis and the like. The molecules have pH probe property, solid-state fluorescent property and binding property with biomacromolecules according to different substituents and anions. Being used as a pH probe, the compound can adjust the pKa of the probe according to the different substituents, and can be used for two-color living cell imaging; being used as a solid-state emission dye, the compound can adjust the solid-state emission wavelength according to different substituents and anions; being used as a biological molecule probe, the compound has good very response to the biological molecule, and living cell membrane permeability ability, can be used for cell staining, can prevent the interference of biomass autofluorescence because of longer absorption and emission wavelengths, and can also reduce the damage of exciting light to cells or biological tissues.

The present invention provides a treatment method to excise a cancerous lesion, such as in the breast, with subsequent ablation of the margin. The method provides for location and excision with ablation under open guidance or guided imaging and for diagnosis by cytology. The method may be a minimally, invasive same day method with diagnosis before or immediately after excision. Also provided is a method of treating close or positive margins of an excisional site of a cancerous lesion in a breast by ablating the margin while monitoring the fluorescence of a fluorophor at the site to determine when ablation of the close or positive margin has occurred.

The invention discloses a transcription factor detection method based on DNA-Ag nanoclusters molecular beacons and exonuclease III cycle signal amplification. The novel nanomaterial DNA-Ag nanoclusters molecular beacon is applied as a fluorescent probe to transcription factor detection for the first time. Also, based on the characteristic that fluorescence adjustment of DNA-Ag nanoclusters molecular beacons is carried out by an enzyme cleavable DNA fragment, exonuclease III is added to participate in signal transformation and also participate in enhancement of the detection signal. The transcription factor detection method provided by the invention has the advantages of no label, high selectivity, low cost and high sensitivity, and can realize high throughput detection of transcription factors in biological samples.

The present invention relates to a method for detecting ferric ions by using fluorescent carbon quantum dots. The method comprises: preparing a dispersion liquid having a mass ratio of glucose and to absolute alcohol of 40:1-5:1; placing into a hydrothermal reaction kettle, and carrying out a reaction for 5-20 h in a 80-230 DEG C environment to obtain fluorescent quantum dots; reactively adding a carbon quantum dot solution to a group of quartz fluorescence cuvettes; adding a ferric ion solution with a concentration of 10<8>-10<5>, placing in a fluorescence spectrophotometer, testing the optimal excitation wavelength of the carbon quantum dots, adjusting the excitation wavelength of the fluorescence spectrophotometer to the optimal excitation wavelength, and recording the change curve of the fluorescence intensity of the solution along with the fluorescence excitation wavelength change; drawing the change curve of the carbon quantum dot solution fluorescence intensity reducing percentage along with the iron ion concentration change, and fitting to obtain a fitting formula; and calculating the ferric ion concentration in the detected object by using the formula. According to the present invention, the method is simple and easy to perform, and other organic reagents are not required to be added.

The present invention relates to a slide having a first and a second major surface on opposite sides of the slide and one or more openings through the slide from the first major surface to the second major surface and one or more membranes mounted to one major surface of the slide over the one or more openings. The slide provides for both an ability to wash and to reduce the background fluorescence in the active membrane area. Preferably the membrane is made of nitrocellulose, nylon or PVDF. The opening(s) can be used to allow wash solutions to pass through the membrane and be removed along with the contaminants (unattached materials such as proteins or antibodies, dyes, salts, etc) from the slide thereby reducing the fluorescent background. Additionally, the one or more openings exhibit a lower fluorescence than a membrane that is laid over or attached to the slide, as neither the slide material or any adhesive is present which would otherwise increase the background fluorescence.

The invention discloses a preparation method of a rare-earth coordination polymer fluorescence probe and an application of the rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection and belongs to the technical field of optical sensing. The preparation method comprises steps as follows: triphosadenine is mixed with a Tris-HCl buffer solution containing Ce<3+>, and the rare-earth coordination polymer fluorescence probe ATP-Ce-Tris is prepared; when H2O2 is present in the solution, Ce<3+> in the ATP-Ce-Tris is oxidized into Ce<4+> by H2O2 to result in fluorescence quenching of the ATP-Ce-Tris, and accordingly, detection of H2O2 is realized; simple and sensitive detection of glucose is further realized through fluorescence quenching of the ATP-Ce-Tris due to H2O2 which is produced through catalytic oxidation of glucose by glucose oxidases.

A method of testing water to detect the presence of at least one chemical or biological warfare agent includes the steps of: a. exposing water to a measuring means for measuring photosynthetic activity of free-living indigenous photosynthetic organisms; b. measuring photosynthetic activity of indigenous photosynthetic organisms occurring naturally in the water to detect the presence of at least one chemical or biological warfare agent in the water.

The present invention relates to a method and a kit for detecting nucleic acid sequence variation using melting curve analysis, especially relates to a method and a kit for detecting nucleic acid sequence variation by melting curve analysis using self-quenched probe. Said method provides the characteristics of the self-quenched probe employed, as well as the corresponding nucleic acid amplification conditions, so that the probe can bind to the amplified target sequence, and variations of the target sequence can be detected by melting curve analysis. The present invention also encompasses a kit assembled according to the method described.

A bioassay employing a first group including a lanthanide ion carrier chelate and a first recognition element, a second group including an antenna ligand and a second recognition element; where the lanthanide ion carrier chelate binds strongly to lanthanide, or the lanthanide ion carrier chelate binds moderately to lanthanide, and an agent complexing the lanthanide ion is additionally employed at a concentration of at least 1 pmol/l. The antenna ligand binds weakly to the lanthanide ion. Analyte recognition by the first recognition element and by the second recognition element results in either chelate complementation and increased fluorescence, or chelate discomplementation and decreased fluorescence.

The invention provides supramolecular polymer gel based on pillar[5]arene. The supramolecular polymer gel has the advantages that the pillar[5]arene modified by sulfo-acethydrazide serves as a main body, 2,2'-(1,6-hexyl)-benzimidazole serves as an object, the main body and the object are completely dissolved and self-assembled in a DMSO-H2O solution and then cooled to room temperature to form theorganic gel with bluish-white aggregation induced emission, the aggregation-state fluorescence quenching is achieved after coordination between the gel and iron or copper ions, and the two types of metal ions are detected highly sensitively; solid powder obtained after drying of the supramolecular polymer gel emits strong white fluorescence, and can be directly used for quickly detecting acid gases, so that a possibility is provided for development and application of high-performance solid gas sensors.

Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.

Adhesive compositions useful for bonding fluorescent material-containing cellulosic substrates such as envelopes may be formulated using an adhesive polymer such as an emulsion of polyvinyl acetate in water and one or more compounds capable of reducing the degree of fluorescence exhibited by the substrate in areas of the substrate surface having the adhesive coated thereon. When irradiated by short wavelength light, the surface areas containing the adhesive coating appear darker than the surface areas that are free of adhesive, allowing quality control problems associated with application of the adhesive to be readily monitored and corrected. At the same time, however, the adhesive may be formulated such that the fluorescence-reducing compound does not alter the appearance of the adhesive coating when viewed under normal daylight conditions.

The invention provides an oil-base illegal cooking oil lubricant for drilling fluids, which comprises the following components in percentage by weight: 90-95% of illegal cooking oil, 0.1-0.15% of preservative, 0.5-1% of antioxidant, 5-7% of extreme-pressure additive, 0.1-0.15% of ultraviolet absorbent and 0.5-1% of pour-point depressant. The oil-base illegal cooking oil lubricant for drilling fluids is developed by using the illegal cooking oil as the base raw oil. The lubricant has the advantages of favorable water solubility, high stability, low fluorescence and no influence on geological logging, effectively solves the problem that the lubrication lowers the torque and friction resistance in the horizontal well drilling process, provides a brand-new way for recovery and reutilization of illegal cooking oil, has wide and profound social benefits, and has wide prospects for popularization and application.

The invention provides a method for synthesizing fluorescent silver nano-particles under microwave by polymethylacrylic acid, relating to fluorescent silver nano-particles, and provides the method for synthesizing the fluorescent silver nano-particles under microwave by polymethylacrylic acid. In the method, the polymethylacrylic acid is used as a stabilizing agent and a reducing agent to synthesize the fluorescent silver nano-particles by a microwave method; silver nitrate aqueous solution is prepared; polymer is added into the silver nitrate aqueous solution, and is stirred to prepare reaction precursor solution; the reaction precursor solution is filled in a quartz reaction flask, the quartz reaction flask is sealed and subject to a microwave heating reaction; and after the microwave heating reaction, the system is cooled to room temperature to obtain the fluorescent silver nano-particles, and the obtained product can be placed in a 4 DEG C low-temperature refrigerator to keep in dark place.

The present invention has an object to provide a novel agent for anti-neurodegenerative diseases and solves the object by providing an agent for anti-neurodegenerative diseases containing, as an effective ingredient, the compound(s) represented by the following General formula 1:

wherein in General formula 1, R₁ through R₃ independently represent a hydrogen atom or an appropriate substituent; Z₁ represents a heterocyclic ring and Z₂ represents the same or different heterocyclic or aromatic ring as in Z₁, wherein the heterocyclic and aromatic rings optionally have a substituent; represents an integer of 0, 1 or 2; p represents an integer of 0 or 1, with the proviso that p is 1 when o is 0 or 2, and p is 0 when o is 1; R₁ and R₂ do not exist when o is 0, while, when p is 0, R₃ does not exist and the binding between the carbon atom to which R₂ binds and Z₂ is a single bond; X₁⁻ represents an appropriate counter anion and q represents an integer of 1 or 2.

The present invention relates to a composition of citric acid based emulsifiers comprising polyamides for oilfield drilling applications that exhibit very low fluorescence, and to a process for their preparation.

Belong to synthesis of functional dyes and their application as fluorescent dyes in the field of fine chemicals, the invention relates to synthesis and application of an indole hemicyanine dye. The compound is formed by condensation of an indole quaternary ammonium salt and corresponding aldehyde, and has the advantages of large molar extinction coefficient, good light stability, and easy synthesis, etc. According to different substituent groups and anions, the molecule involved in the invention can have pH probe properties, solid-state fluorescence properties and the performance to combine with biomacromolecules. When serving as a pH probe, the compound can adjust probe pKa according to different substituent groups, and can be used for bi-color living cell imaging; when serving as a solid-state emission dye, the compound can adjust its solid-state emission wavelength according to different substituent groups and anions; and when serving as a biomolecular probe, the compound has good response to biological molecules and live cell membrane permeability, and can be used for cell staining. Due to its long absorption and emission wavelength, the compound can avoid the interference of biomass autofluorescence, and also reduce the damage of excitation light on cells or biological tissues.