5620 results about "Fluorescence intensity" patented technology

Microspheres are constructed using magnetic particles. Hybrid microspheres are constructed using fluorescent or luminescent microspheres and magnetic nanoparticles. Reactive moieties on the surface of the resultant particles can be used for attachment of biologically active molecules, thus allowing selective separations and analytical assays to be performed. Distinguishable subsets of microspheres can be constructed based on fluorescent intensities, and separations can be affected based on variable degree of magnetic content.

High-accuracy magnetic measurement is performed by efficiently using nitrogen-vacancy pairs in all orientations. A magnetic measurement apparatus includes a diamond crystal and an image sensor. The diamond crystal has nitrogen-vacancy pairs. The image sensor detects the intensities of fluorescence generated by an exciting light applied to the diamond crystal by using a plurality of pixels. The nitrogen-vacancy pairs of the diamond crystal are made to one-to-one correspond to the pixels. The fluorescence generated by one nitrogen-vacancy pair is received by one pixel made to correspond to the nitrogen-vacancy pair.

A magnetic measuring device can be downsized. The magnetic measuring device includes a diamond crystal, a microwave source, a light source array/microwave circuit chip, an image sensor, and a signal controller. The diamond crystal contains a plurality of nitrogen-vacancy pairs. The microwave source generates the microwave that is irradiated to the diamond crystal. The microwave circuit unit in the light source array/microwave circuit chip irradiates the diamond crystal with the microwave. The light source array in the light source array/microwave circuit chip irradiates the diamond crystal with excitation light. The image sensor detects an intensity of fluorescent light generated from the diamond crystal. The signal controller performs image processing of a fluorescent image taken-in by the image sensor, and controls operations of the light source array/microwave circuit chip and the microwave source. The light source array/microwave circuit chip is provided on a first surface side of the diamond crystal, and the image sensor is provided on a second surface side opposed to the first surface of the diamond crystal.

A fluorescent signal analyzing apparatus includes an image input unit, a storage unit, an extracting unit, and an analyzing unit. At least one fluorescent image of a sample is input to the input unit. The storage unit stores data on the at least one fluorescent image input to the image input unit. The extracting unit extracts, for each of the fluorescent images, data on a fluorescence intensity in a desired region on the at least one fluorescent image from the data on the at least one fluorescent image stored in the storage unit. The analyzing unit calculates at least one of a correlation and a photon counting histogram for the desired region by use of the data on the fluorescence intensity.

A fluorescence conversion medium comprising, a fluorescent fine particle formed of an inorganic nanocrystal which absorbs visible and/or near ultraviolet light and emits visible fluorescence, and a transparent medium which disperses the fluorescent fine particle therein, an absorbance at a wavelength where the fluorescent intensity is maximized being in the range of 0.1 to 1.

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.

There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention relates to a method based on near infrared fluorescence molecules and nano particle marks as well as an immunochromatography quantitative detection reagent based on near infrared fluorescence nanoparticles. According to the invention, infrared fluorescence is connected with nanoparticles to prepare an immunochromatography test strip based on the near infrared fluorescence nanoparticles. During the detection, an infrared light scanner is utilized, and a quality control line and a sample line are respectively scanned by utilizing near infrared light; and after the fluorescence intensity of a detection line is rectified by utilizing the fluorescence intensity of a quality control line, and a standard curve in a fluorescence analyzer is taken in, the concentration of a to-be-detected matter in a sample can be analyzed and detected. Compared with the direct connection of the near infrared fluorescence molecules and detecting molecules, the near infrared fluorescence nanoparticle immunochromatography improves the detection flexibility obviously, and the lowers the background fluorescence intensity. The marking method and the reagent can be applied to microorganism detection, food safety detection, poison detection as well as rapid dangerous chemical product detection.

The invention provides a drug residue competition-type quantum dot-labeled immunochromatography assay test-strip/detection card and an observation device thereof, comprising the following preparation steps of coating the antibody of quantum dot-labeled drug molecule on a fiberglass film, coating the drug-molecular-carrier protein coupler and second antibody on a pyroxylin film or a cellulose acetate film respectively to form a detection belt and a quality control belt; preparing the immunochromatography assay test-strip from the fiberglass film and the pyroxylin film or the cellulose acetate film on a polyester or plastic plate, and assembling a shell. The quantum dots of the detection belt and the quality control belt on the test-strip is triggered by the ultraviolet LED source of the observation device, and the change of the fluorescent intensity of the observation belt and the quality control belt is observed, thus being capable of quantitatively analyzing the content of the drug molecule in the sample. The invention has simple operation, high sensitiveness and quick quantification, is suitable for detecting the drug residue in the food and can be widely applied to the customhouse, airport, health supervising department, household and the like.

A method of assaying the antioxidant capacity of a sample, the method including preparing an extraction solution including a solubility enhancing compound, adding the sample to the extraction solution, extracting the antioxidants present in the sample, adding a fluorescent probe to the extract, adding a free radical generator to the extract, detecting the fluorescence intensity decay of the probe in the presence of the sample over time, and calculating the antioxidant capacity of the sample based on the fluorescence intensity decay of the probe in the presence of the sample.

The invention relates to a preparation method and an application of a magnetic fluorescent nanoparticle with a shell-core structure. Firstly, a silica magnetic microsphere with a shell-core structure is prepared by using one or more than one of nanoparticles of Fe3O4, gamma-Fe2O3, MeFe2O4 (Me=Co, Mn, Ni), metal Ni, Co, Fe, and alloy Fe-Co, Ni-Fe as the inner core, and coating a silica shell, and then a fluorescent material (a chelate of Eu3+, Sm3+, Dy3+, Tb3+ and the like) is absorbed on the silica shell. Then, a layer of silica is coated on the surface to improve the stability of the fluorescent magnetic microsphere, and to prevent agglomeration and fluorescent material leakage. A lot of rare earth fluorescent materials are wrapped in the shell layer, so the fluorescence intensity signal of a prepared sample is greatly increased. The nanoparticle has dual functions of enrichment and marking, and has wider application prospects in the biomedical field.

A hand-held, small, lightweight instrument is disclosed that contains a light source capable of producing radiant energy in the spectral range between approximately 370 and approximately 410 nm, and an optical direction system for irradiating a target tissue by producing an illuminated spot thereon. A collection system is provided for receiving and directing fluorescent emissions in the frequency range between 450 and 700 nm returned from the target area to a detector. A processing system is used to determine the state of the tissue by using at least two and up to five spectral bands, preferably each being larger than 45 nm. Pairs of ratios of fluorescent intensities are compared to identify cancerous cells. An alphanumeric, false-color image and/or audio signal is immediately provided to inform a user of the state of the tissue.

The invention discloses a microwave sensor based on an NV color center diamond. A diamond internally containing a Nitrogen-Vacancy color center is adopted as a sensitive element, electronic energy level stimulation is achieved through lasers, an additionally-arranged static magnetic field is scanned, and the microwave frequency and the microwave intensity are measured through fluorescence intensity detection. Dependency of electronic rabi-flopping of the NV color center in the diamond on the external microwave magnetic field is brought into play, high theoretical accuracy and good stability are achieved, the microwave sensor has the advantages of being small in size, low in cost, high in accuracy, large in temperature range, simple in operation condition and the like and rotates on the basis of solid atoms, and the microwave sensor can serve in all the fields with the requirements for the low-cost high-accuracy microwave frequency and intensity detection in the future.

The present invention is to provide a high transmittance glass sheet that has a composition containing as coloring components, expressed in wt. %, 0.005 to less than 0.02% of total iron oxide in terms of Fe₂O₃, less than 0.008% of FeO, and 0 to 0.25% of cerium oxide and having a ratio of FeO in terms of Fe₂O₃ to the total iron oxide of lower than 40%, and exhibits high visible light transmittance and solar radiation transmittance. Alternatively, a high transmittance glass sheet is provided that contains not more than 0.06% of total iron oxide and 0.025 to 0.20% of cerium oxide and has a ratio of a fluorescence intensity at 395 nm to a fluorescence intensity at 600 nm of 10% or higher when subjected to ultraviolet irradiation at a wavelength of 335 nm.

The invention relates to fluorescence immune chromatography test paper which is formed by mutually and sequentially overlapping a sample pad, a combination pad, an antibody carrying film and water absorbing paper on a lining board with adhesive, wherein the combination pad is coated with a fluorescence material antibody 1 composite, a fluorescence-marked material is fluorescein granules or fluorescence material converted by rare earth, the antibody carrying film is a cellulose nitrate film or a nylon film and is respectively connected with an antibody 2 and the T line and the C line of a secondary antibody, the fluorescence material is fluorescein granules or the fluorescence granules converted by the rare earth, and the fluorescein granules are selected from isosulfocyanic acid fluorescein or tetramethyl isosulfocyanic acid rhodamine or tetraethyl rhodamine, and the surface of the fluorescence-marked material is aminated and combined with the antibody by cross-linking reaction. The fluorescence quantitative measuring system is used for detecting the fluorescence strength of the areas of the T line and the C line, and the quantitative detection is completed by the standard curve of the antigen concentration. The invention is suitable for the immune detection of tumor marked objects of urinal fiber-connected protein, and the like.

The invention discloses an ultraviolet fluorescence double-signal water quality monitoring device taking an LED (light emitting diode) as a light source and an application method of the device, and belongs to the field of environmental monitoring and water treatment. The ultraviolet fluorescence double-signal water quality monitoring device taking the LED as the light source consists of a sample collecting part and a detection part. The application method comprises the steps of selecting a deep ultraviolet LED lamp light source with specific wavelength and a photoelectric detector component according to a three-dimensional fluorescence atlas, detecting fluorescence intensity and ultraviolet absorption at the specific wavelength at the same time and calculating a ratio of the fluorescence intensity to the corresponding ultraviolet absorption. According to the device, no chemical reagent is consumed, monitoring of total concentration change of soluble organic matters in a water body can be realized, change of concentration of fluorescence ingredients such as proteins and humus can be reflected at the same time, and the sensitive, quick, efficient, economical, easy and information-rich on-line monitoring device is provided for the water treatment.

A microscope apparatus comprises: a distribution measurement apparatus with respect to an observation region that comprises a fluorescent material that is activated when irradiated with an activating light, and that emits fluorescence when irradiated with an exciting light of a wavelength different from the activating light, obtains a fluorescent picture image through irradiation with the exciting light, and measures a fluorescent intensity distribution; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by repeating an operation wherein the observation region is irradiated with the activating light at the set irradiation intensities, and repeating an operation wherein a fluorescent picture image is obtained by irradiating with the exciting light after the activating light irradiation, generating a sample picture image.

The invention relates to a method for determining the amount of nucleic acid present in a sample, wherein:—a fluorophore is added to the sample,—fluorescence intensities emitted by the fluorophore at least two emission wavelengths in response to light stimulations at least two excitation wavelengths respectively are measured, and—the amount of nucleic acid present in the sample is deduced from the measured fluorescence intensities.

The invention reduces the loss of fluorescence intensity obtained from a specimen to acquire clear fluorescence images when irradiating the specimen with ultrashort-pulse laser light produced by a laser light source. The invention provides a laser-scanning examination apparatus including a laser light source for producing ultrashort-pulse laser light; a laser light source for producing continuous-wave laser light; a measurement head including an optical scanning unit for scanning the laser light on a specimen and an objective optical system; an imaging unit for detecting return light from the specimen in response to the ultrashort-pulse laser light; and an imaging unit for detecting return light from the specimen in response to the continuous-wave laser light. The laser light sources and one imaging unit are connected to the measurement head by an optical fiber, and the other imaging unit is connected to the measurement head by another optical fiber with a larger core diameter.

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

The invention belongs to the fluorescence-coded technology field and in particular relates to a high voltage electric spinning preparation method of multiplex fluorescence coding microsphere. In the method, fluorescent materials (quantum dots material or fluorescent dyes) with different quantities and different fluorescent characteristics are dispersed in polymer solution (or inorganic matter sol). Then, through the high voltage electric spinning process, the coding microsphere with controllable size and adjustable fluorescent strength and lighting wavelength is obtained. The quantum dots material can be used alone or used in mixing way. The fluorescent material can be used alone or used in mixing way. The flurescent material can also be used together with the quantum dots material. The coding microsphere prepared through the invention can provide fluorescent probes in large quantities for the fields of gene expression, protein-protein interaction, simultaneous detection of various diseases, high throughput screening, combinational chemistry, etc. The method of the invention has the advantages of simple operation, wide application, low cost, stable fluorescence performance, and the like, and is provided with good application and expansion value.