695 results about "Fluorescence intensity" patented technology

The invention discloses a multi-mode nonlinear optical microscopic imaging device and system, and the device achieves the two-photon and three-photon excitation imaging of a sample through an integrated light source module, a dispersion compensation module, a scanning light path module, a fluorescence obtaining module, a synchronous control module and a signal processing module. Fluorescence intensity imaging and fluorescence lifetime imaging can be supported at the same time; wherein the scanning light path module is driven by the random mask to realize sparse scanning, so that scanning point locations are effectively reduced, and the exciting light dosage is reduced; the synchronous control module performs time window screening and processing on the fluorescence signal based on the pulse synchronous signal and the position code, so that the signal acquisition efficiency is improved; the signal processing module adopts an FPGA (Field Programmable Gate Array) to carry out real-time image reconstruction and life calculation, so that the imaging speed and quality are ensured; and the master control PC can also perform high-fidelity reconstruction on the sparse sampling image through a deep learning model, so that high-speed, low-light-dose and high-resolution nonlinear fluorescence intensity and life imaging is realized.

The invention provides a full-automatic indirect immunofluorescence interpretation method and system, a storage medium and a program product, and relates to the technical field of indirect immunofluorescence detection.The method comprises the steps that when identification information of a target sample is obtained, a plurality of sealed antigen slides are placed in slide carrying groove positions of a bearing platform in batches, and the target sample is obtained; driving the bearing platform through the transmission mechanism to sequentially move the plurality of antigen slides to the imaging area, and performing positioning and fixing operation on the antigen slides entering the imaging area by using the positioning and clamping device; sequentially carrying out multi-view scanning on the antigen slides entering the imaging area to obtain multi-view fluorescence images, and splicing the fluorescence images to generate a fluorescence panoramic image; performing feature extraction on the panoramic image, determining a fluorescence karyotype category and a target fluorescence intensity value, and determining an antibody titer according to the category, the intensity value and dilution information; and performing association mapping on the titer, the category and the panoramic image according to the identification information to generate a fluorescence interpretation result.

The invention relates to a UCNPs probe-based in-situ dynamic monitoring molecular interaction method, which comprises: S1, providing a UCNPs probe, the core layer of the UCNPs probe being a rare earth up-conversion nanocrystal, the shell layer of the UCNPs probe being an anti-quenching inert material, and the anti-quenching inert material coating the surface of the rare earth up-conversion nanocrystal; the surface of the anti-quenching inert material is modified with ligand molecules, and the ligand molecules are used for being specifically combined with target molecules in a to-be-detected sample; s2, mixing or contacting the UCNPs probe with a to-be-detected sample containing target molecules to construct a to-be-analyzed molecular interaction system; s3, irradiating the molecular interaction system by adopting near-infrared pulse laser, and collecting the change of the fluorescence lifetime tau of the UCNPs along with the time t and the change of the fluorescence intensity I along with the time t in real time to obtain a tau-t curve and an I-t curve; and S4, according to the tau-t curve and the I-t curve, realizing in-situ dynamic monitoring on the molecular interaction state. According to the method, a molecular binding / dissociation event is directly converted into a quantifiable and anti-interference optical signal, and the technical defect that in-situ dynamic monitoring of molecular interaction cannot be achieved in a traditional technology is overcome.

The invention relates to the technical field of skin lesion area reconstruction, in particular to a skin lesion area reconstruction system based on multi-modal image fusion. The system comprises a deep multi-scale feature extraction module, a skin thermal anomaly feature extraction module, a hemodynamic feature extraction module, a depth and boundary feature extraction module, a skin surface feature extraction module, a fluorescence intensity distribution and morphological feature extraction module, a first feature fusion module, a second feature fusion module and a lesion area reconstruction module. According to the method, appearance, heat, blood flow, structure and surface features are extracted from the multi-modal data, then the multi-modal image data are integrated, finally, comprehensive representation and reconstruction of skin lesion appearance, function and structure information are achieved according to the synergistic effect of the multi-modal information, and the accuracy and clinical reference value of lesion area reconstruction are effectively improved.

The embodiment of the invention relates to the technical field of drug detection, in particular to an anti-CD38 antibody drug receptor occupation detection method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is divided into a TEST tube and an FMO tube, a cell surface antibody and an RO detection antibody are added into the TEST tube, only the cell surface antibody is added into the FMO tube, and only the RO detection antibody is added into the FMO tube. The TEST tube and the FMO tube are subjected to sample detection analysis, so that the average fluorescence intensity of RO detection antibodies of the TEST tube and the FMO tube is obtained. And performing RO detection analysis by adopting a dissociation method, taking the tubes which are not combined with the medicine as a total receptor group, taking the tubes which are combined with the medicine as a dissociation receptor group, and calculating an RO detection result according to the average fluorescence intensity of the TEST tube and the FMO tube.

The invention relates to the cross technical field of biological medicine and image processing, and discloses a mesenchymal stem cell aging detection method based on image processing. The method comprises the following steps: automatically collecting bright field and multi-channel fluorescence images in an integrated cell culture monitoring device; after background correction and illumination homogenization, segmenting the cells by using a U-Net network and extracting single cell contours; calculating characteristics such as cell area, roundness, cytoplasmic ratio, nuclear form irregularity index, lysosome fluorescence intensity mean value and distribution entropy; and inputting a gradient boosting decision tree model to judge the unicellular aging state, and evaluating population aging with a 20% positive rate threshold. According to the invention, label-free, non-invasive and high-flux accurate detection is realized, and the method is superior to manual interpretation.

The invention relates to the technical field of fluorescence detection, in particular to a three-dimensional fluorescence detection correction method based on double light sources, and the correction method comprises the following steps: obtaining double light source optical parameters, sample cell geometric parameters and original fluorescence intensity data, the geometric parameters of the sample pool comprise the length, width, height, pool wall reflection coefficient and sample attenuation coefficient of the sample pool; and constructing a light intensity distribution model in a three-dimensional space of the sample cell based on the optical parameters of the double light sources and the geometric parameters of the sample cell. According to the invention, accurate characterization of the light intensity of any spatial point in the sample cell is realized, light intensity deviation caused by various factors is quantified from the source, personalized correction is carried out on each spatial point, the influence caused by optical path difference at different positions, light source fluctuation and element error is effectively offset, and the accuracy and accuracy of the detection result are improved. The consistency of detection values of standard substances with the same concentration in different areas of the sample pool is greatly improved, and the linearity of a standard curve is remarkably optimized.

The invention relates to a gallium oxide defect detection method. The method comprises the following steps: step 1, obtaining exciting light with a proper wavelength; 2, modulating the exciting light obtained in the step 1 so as to enable the exciting light to accurately enter a microscope system with proper energy; 3, irradiating the modulated exciting light to a gallium oxide sample on an electric displacement table through a microscope system, collecting fluorescence generated after the gallium oxide sample is excited through the microscope system, and transmitting the fluorescence to a spectrograph system to obtain a corresponding fluorescence spectrogram under a preset single pixel point position; and 4, setting a spectrograph system to collect the fluorescence intensity under a specific wavelength, and driving the gallium oxide sample to move through an electric displacement table so as to scan the fluorescence intensity of all pixel point positions in a sample detection range, thereby obtaining a gallium oxide defect fluorescence intensity imaging graph in the sample detection range. The method has the advantage of high sensitivity, can realize rapid nondestructive detection, and also reduces the detection cost at the same time.

The invention discloses a divalent nucleic acid aptamer fluorescence detection method based on an exonuclease I auxiliary target circulation strategy, and belongs to the field of analysis and detection.The divalent nucleic acid aptamer is a repetitive sequence composed of two monovalent nucleic acid aptamers and can form double-stranded DNA through base complementary pairing with complementary strand cDNA of the divalent nucleic acid aptamer, and the divalent nucleic acid aptamer can be used for fluorescence detection of the divalent nucleic acid aptamer. The method comprises the following steps: taking a bivalent nucleic acid aptamer as a template, forming a DNA silver nanocluster (DNA-AgNCs) under the action of silver nitrate and sodium borohydride, and when the target okadaic acid exists, combining the bivalent nucleic acid aptamer with the target, so that cDNA can be replaced from double-stranded DNA by the target. Exonuclease I is added to perform enzyme digestion on the cDNA and the divalent nucleic acid aptamer, so that the fluorescence intensity of the DNA-AgNCs can be rapidly reduced. The content of the okadaic acid can be judged by detecting the change of the fluorescence intensity. The invention provides a simple, convenient, rapid, high-sensitivity and high-specificity detection method for okadaic acid detection based on a divalent nucleic acid aptamer for the first time.

The invention belongs to the technical field of temperature sensors, and particularly relates to an up-conversion luminescence temperature sensor based on a plasmon effect and preparation and application thereof. The core of the sensor provided by the invention is a composite structure formed by a gold nanorod array and Er < 3 + > and Yb < 3 + > co-doped NaYF4 up-conversion nanoparticles, and the gold nanorod array is prepared by adopting a two-way aluminum oxide template method and is regularly and orderly arranged in a hexagonal shape. Under the excitation of 980nm near-infrared light, the local surface plasmon resonance effect of the gold nanorods can significantly improve the up-conversion luminescence intensity and accelerate the thermal response rate of the sensor; the ratio type temperature sensing system is constructed by detecting the fluorescence intensity ratio of two emission bands of 515-535 nm and 535-555 nm, self-calibration accurate detection within the temperature range of 303-348 K can be achieved, and the sensor has the advantages of being uniform in structure, controllable in preparation, high in detection sensitivity, high in response speed and the like.

The invention relates to the technical field of biotechnology detection data processing, discloses a PCR detection fluorescence intensity data processing system and method, and aims to solve the problems of low signal-to-noise ratio of fluorescence signals, inaccurate baseline deduction and poor Ct value judgment consistency in the prior art. The method comprises the following steps: processing an original fluorescence signal through adaptive wavelet threshold noise reduction, dynamically determining and correcting a baseline interval, extracting amplification curve characteristic parameters, dynamically adjusting a fluorescence threshold line according to amplification efficiency, identifying an abnormal amplification mode by using a pre-trained deep learning model, and finally integrating results to generate a comprehensive detection report. By adopting the technical scheme, the signal-to-noise ratio of the fluorescence signal and the baseline deduction precision can be remarkably improved, the consistency of the Ct value is improved, multiple abnormal amplification modes are automatically detected, and a full-automatic processing flow is realized.

The invention designs a preparation method of a magnetic molecular imprinting-aptamer sandwich fluorescence sensor for detecting kanamycin. And the magnetic molecular imprinting composite material Fe3O4 (at) UiO-66 (at) MIP with a specific adsorption target object KANA is prepared. When a detection target object KANA exists, the Fe3O4-coated UiO-66-coated MIP can rapidly and specifically capture the KANA in a short time. Then, a DNA single-stranded aptamer marked with FAM is added, after the aptamer and KANA captured on the surface of the Fe3O4 at-UiO-66 at-MIP are subjected to specific recognition and magnetic separation again, an aptamer probe can enter sediment along with the magnetic composite material Fe3O4 at-UiO-66 at-MIP, the fluorescence intensity of supernate is reduced, and quantitative analysis is conducted according to the fluorescence difference value delta F. When the KANA does not exist, the aptamer cannot enter the precipitate, so that the fluorescence signal intensity is not changed. Double recognition is formed through specific binding of an MIP imprinting cavity and the aptamer, matrix interference is reduced by means of fluorescence characteristics and magnetic separation capacity, the method has the advantages of being high in adsorption capacity, specific in recognition, high in sensitivity, wide in detection range, good in selectivity and the like, and rapid and accurate detection of KANA in a complex sample can be achieved.

The invention provides a high-enthalpy shock tunnel free flow electron density two-dimensional diagnosis system and method. The system comprises a laser, a sheet light assembly, a double-camera imaging system, a time sequence synchronizer, a work station and other core components. NO molecules in a flow field are excited by laser to generate fluorescence, two fluorescence images with nanosecond delay are obtained by using a spectroscope and two sets of synchronous acquisition systems, fluorescence lifetime two-dimensional distribution is calculated based on a fluorescence intensity ratio, and then two-dimensional space distribution of electron density is obtained through inversion of a physical model. Non-contact measurement is achieved, interference of a traditional probe on a flow field is avoided, the method has the outstanding advantages of two-dimensional visualization, high temporal-spatial resolution, high measurement precision and the like, and a reliable diagnosis means is provided for high-enthalpy flow field characteristic research and aircraft communication performance evaluation.

The invention relates to the field of water quality detection, in particular to a water quality sampling detection method and device applied to a small and micro wetland unit. The method comprises the following steps: determining a turbidity coefficient according to scattered light intensity of a target water sample under different wavelengths, absorbance of a target interval in an ultraviolet absorption spectrum and fluorescence intensity of a target area in a fluorescence spectrum; determining a scattering equivalent absorption value of each wavelength according to the scattered light intensity of the target water sample under each wavelength, the absorbance corresponding to each wavelength in the ultraviolet absorption spectrum and the turbidity coefficient; determining a real ultraviolet absorption signal of each wavelength according to the absorbance corresponding to each wavelength in the ultraviolet absorption spectrum and the scattering equivalent absorption value of the wavelength; correcting the fluorescence spectrum according to the turbidity coefficient and the scattering equivalent absorption value of each wavelength to obtain a corrected fluorescence spectrum; and detecting the water quality according to the corrected fluorescence spectrum and the real ultraviolet absorption signal of each wavelength. According to the invention, accurate water quality sampling detection can be provided.

The invention discloses an anti-caries early warning method and system based on oral health detection, and belongs to the technical field of data processing. The method comprises the following steps: acquiring and processing target detection data to generate a clear enamel demineralization feature image and a buffer capability score; analyzing pixel-level fluorescence intensity distribution by adopting a convolutional neural network model, determining a quantized value of the enamel demineralization degree, and further determining a comprehensive dental caries risk level category; judging a risk change trend according to the historical target detection data sequence to obtain a dynamic evaluation update value; a data fusion algorithm is adopted to obtain a final decayed tooth risk assessment index, and risk early warning information is generated according to the early warning template; by integrating fluorescence imaging, an image enhancement algorithm, a convolutional neural network, spectral analysis, k-means clustering and time sequence analysis, a complete process from enamel demineralization feature extraction to comprehensive risk assessment is constructed, the accuracy and individuation level of decayed tooth risk assessment are remarkably improved, and a scientific basis is provided for early prevention and intervention.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The application discloses a pinacine alkyl tetrahydrocarbazole fluorescent probe capable of detecting cell lysosome polarity and a preparation method and application thereof. 9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-formaldehyde is used as raw material, and condensation reaction is carried out with 1,3-indan dione to obtain compound 2-(2-(9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-yl)vinyl)-1-indene-1,3(2H)-dione (TC-AP-ID), and the probe can specifically detect the polarity of cell lysosomes. In a mixed system of 1,4-dioxane and water, with the increase of the polarity of the system, the fluorescence color of the probe gradually changes from orange to red, and the fluorescence intensity significantly decreases. The probe has the advantages of simple synthesis, good selectivity, high sensitivity, good biocompatibility, low toxicity and the like, can be applied to targeted detection of the polarity of cell lysosomes, and has a good application prospect.

The application belongs to the technical field of environmental catalysis and green chemical industry, and particularly relates to a PDI / PMSO composite photocatalytic system and a method for producing hydrogen peroxide by using organic wastewater. The PDI / PMSO composite photocatalytic system can significantly improve the yield of H2O2, and when the concentration of PMSO is 10 mM, the yield of H2O2 reaches 137.28 muM.g ‑1 ·h ‑1 -1.h-1, which is 4.3 times higher than that of a pure water system. The system can promote the separation of photo-generated electron-hole pairs (the steady-state fluorescence intensity is reduced by 37%) and enhance the electron conduction efficiency (the photocurrent is increased by 1.8 times) through synergistic effect, and has excellent cycle stability (the yield is maintained above 80% after 10 cycles) and practical water body applicability. The process is simple and low in cost, and provides an integrated solution for the resource utilization of organic wastewater and the green synthesis and application of H2O2.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The application discloses a detection method of cell surface antigens and application thereof, and relates to the detection field. The detection method of cell surface antigens comprises the following steps: mixing liposome-treated cells to be detected with capture microspheres and fluorescent microspheres, detecting the fluorescence intensity, and analyzing the expression of cell surface antigens. The surface of the capture microspheres is coated with a first antibody against a specific antigen on the surface of target cells. The surface of the fluorescent microspheres is coated with a second antibody against a target antigen on the surface of target cells. The detection method has low cost and can be used for directly quantitatively and qualitatively analyzing the expression of surface antigens of specific types of cells.

The invention belongs to the field of biosensing and fluorescence imaging, and particularly relates to a fluorescent probe compound and application thereof. The probe compound is a compound as shown in a formula I or a compound as shown in a formula II, or a pharmaceutically acceptable salt thereof; the structures of the compound in the formula I and the compound in the formula II are shown in the specification, and the definition of each group is shown in any embodiment of the invention. The fluorescent probe compound shows rapid and high reactivity to oxidizing free radicals, the generated fluorescent compound is high in fluorescence intensity and good in stability, and early diagnosis can be carried out on ferroptosis-related disease models such as oxygen-glucose deprivation / reperfusion and drug liver injury on living cell or living body levels and the like. I II

This invention discloses a method for determining the arsenic content in ores, belonging to the field of ore element detection technology, comprising the following steps: (1) weighing the ore sample, adding hydrochloric acid-nitric acid mixed acid for acid hydrolysis, then adding hydrofluoric acid and perchloric acid for auxiliary digestion, evaporating to near dryness, and dissolving the residue with hydrochloric acid; (2) adding a thiourea-ascorbic acid-citric acid mixed reducing masking agent to reduce and mask interfering ions; (3) using an atomic fluorescence spectrometer to determine the fluorescence intensity of arsenic in the sample solution, and calculating the arsenic content according to the standard curve. This method achieves accurate and stable determination of arsenic in various ores such as phosphate rock and iron ore by optimizing sample pretreatment conditions, improving the reduction system and matrix interference suppression strategy.

This invention belongs to the field of fiber optic sensing and relates to a method based on WS 2 A temperature sensor based on all-fiber fluorescence intensity comprises a single-mode fiber, a three-hole grapefruit fiber, and a multimode fiber connected in sequence; the three-hole grapefruit fiber has three air holes, in which WS is deposited. 2 The multimode fiber output end is equipped with a mounting base, on which a filter is mounted. This invention utilizes WS... 2 Assembled within a three-hole optical fiber, with single-mode and multi-mode fibers fused together at both ends, this sealed structure effectively isolates the sensor from external air, preventing contact between the outside air and the two-dimensional material on the inner wall of the fiber during heating. This slows down the decomposition of transition metal sulfides, significantly improving the fluorescent sensor's ability to detect high temperatures and extending its lifespan. The sensor itself and its detection system are coupled and output via optical fiber, eliminating the need for external spatial light or optical modulation.

The application discloses an acid-responsive fluorescent sensing material and a preparation method and application thereof. The acid-responsive fluorescent sensing material can detect strong acid in a liquid environment. The structure of the acid-responsive fluorescent sensing material is as follows: the group responding to acid of the material is a hydroxyl group of a tertiary alcohol, and the hydroxyl group is removed under the action of acid to change the fluorescence intensity. Different molar amounts of strong acid can be distinguished by different fluorescence intensities in various liquid environments. The fluorescent sensing material prepared by the application can detect acid. After the fluorescent sensing material is mixed with acid, the color of the solution can be obviously observed to change. The change of the fluorescence signal under an ultraviolet lamp can be observed by naked eyes. The visual detection of strong acid is realized. The response speed and accuracy of the acid are improved. The fluorescent sensing material has good reusability. The acid-responsive fluorescent sensing material has the advantages of simple synthesis method, convenient separation and purification, high yield and good stability, and therefore has certain application value in the field of intelligent sensors.

The application discloses a lateral test strip for detecting C-reactive protein and application thereof, and the test strip comprises a bottom plate and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption paper which are sequentially stacked on the bottom plate; the combination pad is loaded with AIE fluorescent microspheres; the AIE fluorescent microspheres are composed of P(St-MMA) microspheres coated with AIE materials after hydrolysis and carboxylation; and anti-C-reactive protein antibodies are connected to the AIE fluorescent microspheres through a covalent bond. The surface coating amount of the AIE fluorescent microspheres is increased by 1.6-2.3 times, and the fluorescence intensity per unit mass is increased by about 40-60%. The increase of the coating amount makes the density of the light-emitting core higher, the overall quantum yield is significantly improved, and the uniformity of the fluorescence signal and the detection sensitivity are enhanced. The characteristics are particularly prominent in the detection of CRP protein in the chromatographic test strip, and the detection limit can be effectively reduced, the T / C signal ratio can be improved, and the reaction time can be shortened.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

The invention provides a method for detecting sulfonamides in animal-derived food through capillary electrophoresis-laser-induced fluorescence, and belongs to the technical field of detection. Comprising the following steps: (1) mincing a sample, adding an extracting solution for extraction, and carrying out solid-phase extraction column extraction and methanol elution to obtain an eluted sample solution; (2) preparing a sulfanilamide drug standard substance and fluorescamine into gradient solutions by using methanol and acetonitrile respectively, and stirring at room temperature for reaction to obtain a solution A; (3) mixing the elution sample solution with a fluorescent amine-acetonitrile solution, and stirring and reacting at room temperature to obtain a solution B; and (4) detecting the fluorescence intensity of the sulfonamides in the solution A by adopting capillary electrophoresis-laser induced fluorescence to obtain a linear equation for detecting the concentration of the sulfonamides in the solution B. The invention establishes a novel method for detecting sulfonamide residues in animal-derived food by using high-efficiency capillary electrophoresis-laser-induced fluorescence, and realizes high-sensitivity detection of three sulfonamides in animal-derived food.

The invention belongs to the technical field of chrysin detection, and particularly relates to a dual-emission fluorescent molecularly imprinted polymer as well as a preparation method and application thereof. The method comprises the following steps: preparing carbon dots with different emission wavelengths; carrying out amination modification on the carbon dots; and preparing the dual-emission fluorescent molecularly imprinted polymer. The method also comprises the following steps: respectively adding the dual-emission fluorescent molecularly imprinted polymer into chrysin solutions with different known concentrations, carrying out fluorescence quenching reaction, and establishing a standard curve according to the linear relationship between the detected fluorescence intensity and the corresponding concentration of the chrysin solution; adding the dual-emission fluorescent molecularly imprinted polymer into a solution of a to-be-detected product, and carrying out fluorescence quenching reaction to obtain fluorescence intensity; and calculating the concentration of chrysin in the solution to be detected according to the standard curve. The preparation method is used for solving the technical problems that in the prior art, a single-emission fluorescent molecularly imprinted polymer has defects in the aspects of sensitivity, background fluorescence interference resistance in a complex environment and the like in the detection process.

The application provides an adenosine deaminase content detection method and application thereof. The adenosine deaminase content detection method comprises the following steps: using the intrinsic fluorescence of adenosine, using adenosine deaminase to hydrolyze adenosine, and detecting the content of adenosine deaminase by a fluorescence analysis method. Adenosine itself has strong fluorescence, and after being hydrolyzed by adenosine deaminase, the fluorescence of the obtained hydrolysis product is reduced, thereby causing the fluorescence intensity of the solution of the detection system to be reduced; the content of adenosine deaminase in the system is detected by the change of the fluorescence intensity before and after adenosine hydrolysis. The adenosine deaminase content detection method in the application is simple to operate, does not need large instruments and equipment, and is cheap and easy to obtain materials, can accurately and rapidly detect the content of adenosine deaminase in a sample to be detected, has good sensitivity and specificity, and has a relatively wide application prospect.