Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method

Abstract

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a preparation method and a quantitative detection method of a fluorescent microsphere immunochromatography test strip. Background technique [0002] In biological and medical testing, immunoassays are often used, including radioimmunoassays, enzyme-linked immunoassays, and immunochromatography. Radioimmunoassay and enzyme-linked immunoassay require expensive equipment, professional operators and complicated operation steps, and it is difficult to obtain test results quickly. Immunochromatography is often used for rapid qualitative or semi-quantitative detection due to its simple operation, rapidity and low cost, such as colloidal gold test strips such as clenbuterol hydrochloride, hepatitis B surface antigen, aflatoxin and luteinizing hormone. At present, the incidence of common infectious diseases such as AIDS, hepatitis B, hepatitis C, and syphilis in my country continues t...

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Problems solved by technology

[0004] (1) The colloidal gold labeling process is an electrostatic adsorption process, which belongs to physical adsorption, and has poor stability in the liquid phase, and the labeled protein molecules are easy to fall off again;

[0005] (2) Only when the gold particles reach a certain amount, the naked eye can distinguish the difference between the detection band and the background, so the detection sensitivity is not high;

[0006] (3) The matrix effect of different materials is obvious, and the background interference is very large;

The methods disclosed in these patents can improve the sensitivity of immunochromatographic detection, but the reported fluorescent nanoparticles have certain defects, such as dyes are easy to leak, and the ability to resist solution interference is poor, so the scope of application is largely limited.

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