11 results about "BORATE BUFFER" patented technology

This invention discloses a bifunctional small-molecule PEG-modified recombinant humanized collagen and its preparation method. The method includes the following steps: preparing a borate buffer solution containing sodium cyanoborohydride, pH 7.5-8.5; dissolving lyophilized recombinant collagen powder in the buffer solution to obtain a substrate solution; adding crosslinking agent powder to the substrate solution according to a molar ratio of free lysine to crosslinking agent of 1:(1.5-4.0); the crosslinking agent is a bifunctional small-molecule PEG with PEG as the backbone and groups at both ends that can couple with the amino groups of collagen; reacting at 4°C in the dark for 2-5 hours. This invention not only overcomes the bottleneck of anti-enzymatic stability but also transforms recombinant humanized collagen into a multi-arm macromolecular crosslinking substrate with highly active groups on its surface, providing key chemical bonding sites for its subsequent targeted coupling or anchoring to polymer networks such as hydrogels.

The invention provides a nano-enzyme synthesis method which comprises the following specific steps: sequentially heating, drying and grinding an aqueous solution containing 0-generation polyamide-amine dendritic macromolecules, potassium ferricyanide and urea, carrying out high-temperature pyrolysis twice, and grinding again to obtain the target nano-enzyme. The invention also provides a corresponding dichlorvos detection method, which comprises the following operation steps: mixing a sample solution, a buffer solution and a nano-enzyme solution for incubation, and then adding the mixed solution into a borate buffer solution containing hydrogen peroxide for reaction; and after the reaction is finished, sequentially adding a potassium iodide solution and a rhodamine 6G solution, and carrying out fluorescence detection after the reaction is finished. The nano-enzyme synthesized by the method has multiple enzymatic characteristics of oxidase-like, peroxidase-like, catalase-like and superoxide dismutase-like, has the advantages of excellent selectivity and high sensitivity in detection of dichlorvos, and is suitable for rapid detection of dichlorvos in actual samples.

A method of inhibiting corrosion of a metal surface in contact with an aqueous medium is provided. The method may include contacting the metal surface with a corrosion inhibitor composition where the corrosion inhibitor composition may include a compound or salt thereof of formula (I). The disclosed corrosion inhibitor composition is especially useful for inhibiting corrosion in closed loop systems.

A method of inhibiting corrosion of a metal surface in contact with an aqueous medium is provided. The method may include contacting the metal surface with a corrosion inhibitor composition where the corrosion inhibitor composition may include a compound or salt thereof of formula (I). The disclosed corrosion inhibitor composition is especially useful for inhibiting corrosion in closed loop systems.

Disclosed herein is an aqueous composition comprising 0.001-0.1% (w / v) atropine or a salt thereof, a water-soluble polymer, and buffer (I), which is at a pH range of 6 or lower, wherein the buffer (I) is at least one selected from the group consisting of a phosphate buffer, an aminocarboxylate buffer, a carbonate buffer, an acetate buffer, a tartrate buffer, a borate buffer, and trometamol.

The invention discloses a high-chromium stainless steel passive film semiconductor characteristic testing solution and application thereof, and belongs to the technical field of electrochemical testing. The test solution is a borate composite electrolyte system and is prepared from the following components: 0.1 M of Na2B4O7. 10H2O, 0.05 M of H3BO3, 0.1 to 0.3 M of Na2SO4, 0.001 to 0.01 M of citric acid and 0.001 g / L of PEG-4000 (Polyethylene Glycol); na2SO4 is added as a supporting electrolyte, so that the solution resistance is reduced; the high complexing constant of citric acid and Fe < 3 + > is competitively combined with metal ions on the surface of the passive film, so that the damage to the passive film is reduced. The test solution is used for testing the semiconductor characteristics of the high-chromium stainless steel passive film, the problems that a traditional borate buffer solution system is poor in conductivity, high in passive film damage rate, single in environment simulation function and the like are solved, the test precision and functionality of the test solution are remarkably improved through multi-component collaborative optimization and process innovation, and the test cost is lowered. The method is suitable for material performance evaluation in corrosion sensitive environments such as nuclear power and chemical equipment.

The application discloses a method for detecting deoxy-najinmycin in milk and dairy products. The method comprises the following steps: extracting a sample solution to be derivatized by using an extracting agent, adding a buffer solution and a derivatization reagent to the sample solution to be derivatized to perform derivatization, performing liquid chromatography test on the sample to be tested after the derivatization, and qualitatively and quantitatively determining the sample according to the retention time and external standard method. The content of deoxy-najinmycin in the sample to be tested is obtained according to the test result. The extracting agent is an acetonitrile aqueous solution, the buffer solution is a potassium borate buffer solution, and the derivatization reagent comprises a fluorenylmethyloxycarbonyl chloride acetonitrile solution, a glycine solution and an acetic acid aqueous solution. The precision, repeatability, recovery rate and standard curve linearity of the detection method meet the industry requirements. The method has good extraction effect, high sensitivity, stable and accurate results, and is simple to operate and convenient to popularize.

The application discloses a kind of super-hydrophobic activated carbon materials, preparation method and application, it is related to activated carbon production technical field, including the following mass parts of raw materials: 70-90 parts activated carbon, 5-15 parts silicon dioxide nanoparticles, 1-5 parts fluorosilane, 3-7 parts reinforcing modifier.The application adds reinforcing modifier in raw materials, dopamine will occur self-polymerization and form polydopamine in alkaline borate buffer solution, by the active functional groups such as a large number of catechol and amino contained in polydopamine, when pretreated activated carbon B is immersed in reinforcing modifier, polydopamine will be deposited on the surface of activated carbon, by active group, more binding sites are provided for subsequent modification particle A self-assembly, enhance the physical adsorption and chemical bonding effect between modification particle A and activated carbon, to enhance the practical use effect of super-hydrophobic activated carbon, and optimize production process in production process, enhance the functionality.

A magnesium oxide powder, wherein 0.2 g of the magnesium oxide powder is put into 50 mL of a borate buffer (pH = 9.18), dispersed by an ultrasonic homogenizer for 3 minutes, then put into a measuring device within 1 minute after the dispersion treatment, and a Zeta potential measurement is performed under conditions where the temperature is set to 25°C and the equilibration time is 2 minutes, and the Zeta potential thus measured is -50 mV or more and -5 mV or less. It is preferable that the circularity of the above-described magnesium oxide powder be 0.6 or more and 0.8 or less. It is also preferable that the particle diameter Dv50 of the above-described magnesium oxide powder, which is obtained by a laser diffraction scattering method, be 1 μm or more and 200 μm or less. 50 1 μm or more and 200 μm or less.

This invention discloses a method for detecting rapidly soluble branched-chain amino acid free monomers. The method involves preparing water, acetonitrile, methanol, hydrochloric acid solution, acetic acid solution, sodium hydroxide solution, OPA solution, borate buffer, acetate buffer I, acetate buffer II, leucine reference standard, isoleucine reference standard, and valine reference standard; a high-performance liquid chromatograph, a liquid chromatography processor, a chromatographic column, an analytical balance, and centrifuge tubes; the detection steps are sequentially: “preparation of reference solution,” “preparation of sample solution,” “determination of sample solution and reference solution,” and “result calculation.” This provides a highly efficient quantitative detection scheme for rapidly soluble branched-chain amino acid free monomers.

The application relates to the field of pathogenic bacteria adsorption materials, in particular to a klebsiella pneumoniae adsorption film, a preparation method thereof and application of the klebsiella pneumoniae adsorption film in an adsorption column. The preparation method of the klebsiella pneumoniae adsorption film comprises the following steps: preparing an activation solution, incubating a base film, performing oscillation reaction and quenching. The oscillation reaction is that the base film is centrifuged, the supernatant is discarded, then the base film is washed, resuspended in a PBS buffer solution or a borate buffer solution, an aptamer solution is added, and oscillation reaction is performed to obtain an adsorption film semi-finished product. The aptamer in the aptamer solution is a klebsiella pneumoniae aptamer and a klebsiella pneumoniae aptamer expressing carbapenemase 2 (KPC-2). The two kinds of aptamers are synergistically adsorbed, common type and drug-resistant type strains of klebsiella pneumoniae are covered, and the klebsiella pneumoniae adsorption film has strong adsorption effects on the adsorption films of the two kinds of strains.