19 results about "BORATE BUFFER" patented technology

The invention discloses a hemispherical glass precision grinding and polishing system and a grinding and polishing method, and relates to the technical field of hemispherical glass grinding and polishing. The hemispherical glass is finely ground by means of a bionic honeycomb grinding disc, the bionic honeycomb grinding disc is filled with 0.5 micron CeO2 grinding materials, and the pore diameter is 50 microns; the upper disc of the bionic honeycomb grinding disc revolves at 200rpm, and the lower disc of the bionic honeycomb grinding disc reversely rotates at 150rpm; a borate buffer solution is used for lubrication; and finally, the hemispherical glass is polished. Preparing a magnetorheological polishing solution by virtue of 20 vol% of carbonyl iron powder, 2 vol% of nano diamond and glycerol-based carrier liquid; a rigid magnetic brush is formed under the action of a 0.5 T axial magnetic field; meanwhile, the pressure is dynamically adjusted to 0.01-0.03 MPa by means of an interferometer, and the grinding and polishing process and parameter setting are optimized; by means of the method, the machining efficiency and the surface quality are remarkably improved.

This invention discloses a bifunctional small-molecule PEG-modified recombinant humanized collagen and its preparation method. The method includes the following steps: preparing a borate buffer solution containing sodium cyanoborohydride, pH 7.5-8.5; dissolving lyophilized recombinant collagen powder in the buffer solution to obtain a substrate solution; adding crosslinking agent powder to the substrate solution according to a molar ratio of free lysine to crosslinking agent of 1:(1.5-4.0); the crosslinking agent is a bifunctional small-molecule PEG with PEG as the backbone and groups at both ends that can couple with the amino groups of collagen; reacting at 4°C in the dark for 2-5 hours. This invention not only overcomes the bottleneck of anti-enzymatic stability but also transforms recombinant humanized collagen into a multi-arm macromolecular crosslinking substrate with highly active groups on its surface, providing key chemical bonding sites for its subsequent targeted coupling or anchoring to polymer networks such as hydrogels.

The application discloses a quercetin-chitosan nanoparticle suspension eye drop, a preparation method and application thereof. The eye drop contains the following components on the basis of 100ml of preparation: quercetin 0.001-0.006g; chitosan 0.05-0.3g; sodium tripolyphosphate 0.01-0.05g; sodium chloride 0.6-1.5g; benzalkonium bromide 0.005-0.02g; borate buffer 1-3g; and the rest is injection water, and the total amount is 100ml. Quercetin is loaded in nanoparticles formed by ion cross-linking of chitosan and sodium tripolyphosphate. The application solves the technical defects of poor water solubility and easy oxidation of quercetin by constructing a polyphenol-chitosan nanocomposite system. The application has a dual synergistic mechanism of ROS clearance and mucosa repair, significantly improves the ocular bioavailability of quercetin, and has a wide application prospect in drugs for preventing or treating dry eye.

The invention relates to the field of contact lens care, in particular to a hydrogen peroxide care solution suitable for a platinum alloy neutralization ring. The care solution is prepared from the following components in parts by weight: 20 to 50 parts of hydrogen peroxide, 7.6 to 11.3 parts of borate buffer agent, 0.1 to 0.5 part of chelating agent, 6 to 12 parts of nano inclusion solution, 20 to 40 parts of solubilizer, 0.01 to 0.05 part of antioxidant, 5 to 10 parts of regulator and 1000 parts of distilled water. The hydrogen peroxide care solution provided by the invention is suitable for a platinum alloy neutralization ring, and a hydrogen peroxide stabilizing system, a dibazol slow-release system and other auxiliaries are added into the hydrogen peroxide care solution. The care solution can prolong the life of a platinum ring, and the corrosion rate of platinum alloy is reduced by more than 50%; the production of strong acid neutralization products is avoided, and the eye irritation is reduced; in addition, the antibacterial broad-spectrum property is also realized.

The invention relates to the technical field of in-vitro detection reagents, in particular to a 5 '-nucleotidase calibrator and a preparation method thereof.The calibrator comprises 5'-nucleotidase, a borate buffering agent and animal serum, and the animal serum is selected from at least one of newborn calf serum, fetal calf serum, calf serum or sheep serum. The calibrator disclosed by the invention shows good stability and interchangeability, and ensures the detection accuracy of 5 '-nucleotidase.

The application discloses a cell membrane capillary whole column and a preparation method and application thereof, and the preparation method is as follows: (1) preparing a cell membrane suspension; (2) pretreating a capillary; (3) preparing a modified capillary whole column; (4) injecting an HCl aqueous solution into the modified capillary whole column, replacing methanol and reacting, injecting an NaIO4 aqueous solution, oxidizing, washing with water, and balancing with a borate buffer; and injecting the cell membrane suspension obtained in the step (1), replacing the borate buffer, so that a cell membrane capillary whole column is obtained. The cell membrane capillary whole column is used for separating active ingredients of traditional Chinese medicines, does not need to balance a chromatographic column, and is simple and easy to operate. In the process of discovering active chemical ingredients, unnecessary separation steps can be avoided, and the separation period is shortened in the research of effective substances of traditional Chinese medicines. Compared with a traditional separation and identification method, the method is more suitable for the research of effective substances of traditional Chinese medicines containing complex components.

The invention provides a nano-enzyme synthesis method which comprises the following specific steps: sequentially heating, drying and grinding an aqueous solution containing 0-generation polyamide-amine dendritic macromolecules, potassium ferricyanide and urea, carrying out high-temperature pyrolysis twice, and grinding again to obtain the target nano-enzyme. The invention also provides a corresponding dichlorvos detection method, which comprises the following operation steps: mixing a sample solution, a buffer solution and a nano-enzyme solution for incubation, and then adding the mixed solution into a borate buffer solution containing hydrogen peroxide for reaction; and after the reaction is finished, sequentially adding a potassium iodide solution and a rhodamine 6G solution, and carrying out fluorescence detection after the reaction is finished. The nano-enzyme synthesized by the method has multiple enzymatic characteristics of oxidase-like, peroxidase-like, catalase-like and superoxide dismutase-like, has the advantages of excellent selectivity and high sensitivity in detection of dichlorvos, and is suitable for rapid detection of dichlorvos in actual samples.

The invention relates to the field of pathogen adsorption materials, in particular to a klebsiella pneumoniae adsorption film, a preparation method thereof and application of the klebsiella pneumoniae adsorption film in an adsorption column. A preparation method of a klebsiella pneumoniae adsorption membrane comprises the following steps: preparing an activating solution, incubating a matrix membrane, carrying out an oscillating reaction and quenching, in the oscillating reaction, centrifuging the matrix membrane, removing a supernatant, cleaning, re-suspending in a PBS buffer solution or a borate buffer solution, adding an aptamer solution, and carrying out the oscillating reaction to obtain a semi-finished product of the adsorption membrane; the aptamers in the aptamer solution are a klebsiella pneumoniae aptamer and a klebsiella pneumoniae aptamer for expressing carbapenemase 2 (KPC-2). According to the application, common and drug-resistant strains of klebsiella pneumoniae are covered by virtue of synergistic adsorption of the two aptamers, so that the klebsiella pneumoniae adsorption film has a relatively strong adsorption effect on adsorption films of the two strains.

The application provides a preparation method of a functionalized gel for capillary electrophoresis analysis, comprising the following steps: configuring a potassium borate buffer solution containing tris-hydroxymethyl aminomethane, ethylenediamine tetraacetic acid and anhydrous potassium tetraborate; adding a solid polymer product into the potassium borate buffer solution, and dissolving at room temperature to obtain the functionalized gel; mixing a sugar aqueous solution, a labeled molecule acetic acid solution and a reducing agent mixture according to a specified proportion, and incubating at a specified temperature to obtain a derivatized monosaccharide sample, and diluting the derivatized monosaccharide sample; mixing the derivatized monosaccharide sample with formamide according to a specified proportion to obtain a sample to be detected, filling a capillary with the functionalized gel, using the potassium borate buffer solution as a buffer solution at both ends of the capillary, and separating on a capillary electrophoresis instrument according to specified electrophoresis conditions.

A method of inhibiting corrosion of a metal surface in contact with an aqueous medium is provided. The method may include contacting the metal surface with a corrosion inhibitor composition where the corrosion inhibitor composition may include a compound or salt thereof of formula (I). The disclosed corrosion inhibitor composition is especially useful for inhibiting corrosion in closed loop systems.

A method of inhibiting corrosion of a metal surface in contact with an aqueous medium is provided. The method may include contacting the metal surface with a corrosion inhibitor composition where the corrosion inhibitor composition may include a compound or salt thereof of formula (I). The disclosed corrosion inhibitor composition is especially useful for inhibiting corrosion in closed loop systems.

Disclosed herein is an aqueous composition comprising 0.001-0.1% (w / v) atropine or a salt thereof, a water-soluble polymer, and buffer (I), which is at a pH range of 6 or lower, wherein the buffer (I) is at least one selected from the group consisting of a phosphate buffer, an aminocarboxylate buffer, a carbonate buffer, an acetate buffer, a tartrate buffer, a borate buffer, and trometamol.

A magnesium oxide powder in which 0.2 g of a magnesium oxide powder is introduced into 50 mL of a borate buffer solution (pH = 9.18), is dispersed for 3 minutes by an ultrasonic homogenizer, then is introduced into a measurement device within 1 minute after the dispersion, and is subjected to Zeta potential measurement under the conditions of a set temperature of 25 DEG C and an equilibrium time of 2 minutes, and the measured Zeta potential is-50 mV to-5 mV (inclusive). It is preferable that the magnesium oxide powder have a circularity of 0.6 to 0.8 inclusive. It is also preferable that the particle diameter (D50) of the magnesium oxide powder at a volume-based cumulative frequency of 50% obtained by laser diffraction scattering is 1 [mu] m to 200 [mu] m (inclusive).

The invention discloses a high-chromium stainless steel passive film semiconductor characteristic testing solution and application thereof, and belongs to the technical field of electrochemical testing. The test solution is a borate composite electrolyte system and is prepared from the following components: 0.1 M of Na2B4O7. 10H2O, 0.05 M of H3BO3, 0.1 to 0.3 M of Na2SO4, 0.001 to 0.01 M of citric acid and 0.001 g / L of PEG-4000 (Polyethylene Glycol); na2SO4 is added as a supporting electrolyte, so that the solution resistance is reduced; the high complexing constant of citric acid and Fe < 3 + > is competitively combined with metal ions on the surface of the passive film, so that the damage to the passive film is reduced. The test solution is used for testing the semiconductor characteristics of the high-chromium stainless steel passive film, the problems that a traditional borate buffer solution system is poor in conductivity, high in passive film damage rate, single in environment simulation function and the like are solved, the test precision and functionality of the test solution are remarkably improved through multi-component collaborative optimization and process innovation, and the test cost is lowered. The method is suitable for material performance evaluation in corrosion sensitive environments such as nuclear power and chemical equipment.

The application discloses a method for detecting deoxy-najinmycin in milk and dairy products. The method comprises the following steps: extracting a sample solution to be derivatized by using an extracting agent, adding a buffer solution and a derivatization reagent to the sample solution to be derivatized to perform derivatization, performing liquid chromatography test on the sample to be tested after the derivatization, and qualitatively and quantitatively determining the sample according to the retention time and external standard method. The content of deoxy-najinmycin in the sample to be tested is obtained according to the test result. The extracting agent is an acetonitrile aqueous solution, the buffer solution is a potassium borate buffer solution, and the derivatization reagent comprises a fluorenylmethyloxycarbonyl chloride acetonitrile solution, a glycine solution and an acetic acid aqueous solution. The precision, repeatability, recovery rate and standard curve linearity of the detection method meet the industry requirements. The method has good extraction effect, high sensitivity, stable and accurate results, and is simple to operate and convenient to popularize.

The application discloses a kind of super-hydrophobic activated carbon materials, preparation method and application, it is related to activated carbon production technical field, including the following mass parts of raw materials: 70-90 parts activated carbon, 5-15 parts silicon dioxide nanoparticles, 1-5 parts fluorosilane, 3-7 parts reinforcing modifier.The application adds reinforcing modifier in raw materials, dopamine will occur self-polymerization and form polydopamine in alkaline borate buffer solution, by the active functional groups such as a large number of catechol and amino contained in polydopamine, when pretreated activated carbon B is immersed in reinforcing modifier, polydopamine will be deposited on the surface of activated carbon, by active group, more binding sites are provided for subsequent modification particle A self-assembly, enhance the physical adsorption and chemical bonding effect between modification particle A and activated carbon, to enhance the practical use effect of super-hydrophobic activated carbon, and optimize production process in production process, enhance the functionality.

A magnesium oxide powder, wherein 0.2 g of the magnesium oxide powder is put into 50 mL of a borate buffer (pH = 9.18), dispersed by an ultrasonic homogenizer for 3 minutes, then put into a measuring device within 1 minute after the dispersion treatment, and a Zeta potential measurement is performed under conditions where the temperature is set to 25°C and the equilibration time is 2 minutes, and the Zeta potential thus measured is -50 mV or more and -5 mV or less. It is preferable that the circularity of the above-described magnesium oxide powder be 0.6 or more and 0.8 or less. It is also preferable that the particle diameter Dv50 of the above-described magnesium oxide powder, which is obtained by a laser diffraction scattering method, be 1 μm or more and 200 μm or less. 50 1 μm or more and 200 μm or less.

This invention discloses a method for detecting rapidly soluble branched-chain amino acid free monomers. The method involves preparing water, acetonitrile, methanol, hydrochloric acid solution, acetic acid solution, sodium hydroxide solution, OPA solution, borate buffer, acetate buffer I, acetate buffer II, leucine reference standard, isoleucine reference standard, and valine reference standard; a high-performance liquid chromatograph, a liquid chromatography processor, a chromatographic column, an analytical balance, and centrifuge tubes; the detection steps are sequentially: “preparation of reference solution,” “preparation of sample solution,” “determination of sample solution and reference solution,” and “result calculation.” This provides a highly efficient quantitative detection scheme for rapidly soluble branched-chain amino acid free monomers.

The application relates to the field of pathogenic bacteria adsorption materials, in particular to a klebsiella pneumoniae adsorption film, a preparation method thereof and application of the klebsiella pneumoniae adsorption film in an adsorption column. The preparation method of the klebsiella pneumoniae adsorption film comprises the following steps: preparing an activation solution, incubating a base film, performing oscillation reaction and quenching. The oscillation reaction is that the base film is centrifuged, the supernatant is discarded, then the base film is washed, resuspended in a PBS buffer solution or a borate buffer solution, an aptamer solution is added, and oscillation reaction is performed to obtain an adsorption film semi-finished product. The aptamer in the aptamer solution is a klebsiella pneumoniae aptamer and a klebsiella pneumoniae aptamer expressing carbapenemase 2 (KPC-2). The two kinds of aptamers are synergistically adsorbed, common type and drug-resistant type strains of klebsiella pneumoniae are covered, and the klebsiella pneumoniae adsorption film has strong adsorption effects on the adsorption films of the two kinds of strains.