206 results about "Fluorometric Analysis" patented technology

The invention discloses a full-automatic milk somatic cell detection system and method based on fluorescence analysis. According to the full-automatic fluorescence microscopic examination system, automatic sample introduction and automatic mixing are completed through the automatic sample introduction module, automatic focusing of dyed samples is completed through the automatic focusing module, automatic photographing processing and automatic counting are completed through the automatic photographing and counting module, and therefore full-automatic fluorescence microscopic examination is achieved. According to the method, the automatic detection level is remarkably improved, the consistency of the detection process is ensured, and manual operation errors are reduced, so that the experiment efficiency, the data accuracy and the reproducibility are improved; compared with traditional off-line detection or laboratory detection, the method and system provided by the invention have better real-time performance and accuracy, and also have higher automation degree.

The invention discloses a ratio-type fluorescence analysis method for determining sodium dehydroacetate, which comprises the following steps: under an alkaline condition, synthesizing tetrafluoroterephthalic acid oxide, namely a fluorescent substance TFAox, by oxidizing tetrafluoroterephthalic acid with hydrogen peroxide, and forming a ratio-type fluorescence probe by the fluorescent substance and rhodamine B, a ratio-type fluorescence analysis method of sodium dehydroacetate is constructed based on the ratio-type fluorescence probe, and the method can be successfully applied to determination of sodium dehydroacetate in a water sample and a bread sample. The ratio-dependent fluorescence detection method for determining sodium dehydroacetate provided by the invention has the characteristics of wide linear range, high detection sensitivity, no interference of coexisting substances on determination, good selectivity and the like.

This invention discloses a method, apparatus, and electronic device for microstructure and performance analysis of NiV alloy targets, comprising: determining the grain size in the NiV alloy target based on a high-resolution electron microscope; dividing the NiV alloy target into coarse-grained and fine-grained regions based on the grain size; determining the form window for X-ray fluorescence spectroscopy analysis of each crystal region based on the grain size; applying X-ray fluorescence to each crystal region based on the form window to obtain fluorescence analysis values; comparing the fluorescence analysis values ​​with standard fluorescence values ​​to obtain X-ray fluorescence spectra; determining the vanadium content and sputtering rate of the NiV alloy target in each crystal region based on the X-ray fluorescence spectra; determining the texture of the target in each crystal region based on the sputtering rate of the NiV alloy target in each crystal region and the directional pole figure outside the etching groove of the target; and performing comparative analysis on the target in each crystal region based on the texture of the target in each crystal region to obtain the microstructure and performance analysis results for the target in each crystal region.

The invention provides application of nano CDs as a fluorescent probe in fluorescence detection of sugammadex sodium and a fluorescence detection method of sugammadex sodium, and relates to the technical field of drug analysis and detection. According to the invention, carboxyl in sugammadex sodium and nitrogen atoms in nano CDs interact with each other, the fluorescence intensity of nano CDs is enhanced through intramolecular charge transfer, sugammadex sodium and nano CDs can be effectively combined through a static combination effect, and nano CDs have high sensitivity and selectivity to sugammadex sodium; the method is suitable for detecting sugammadex sodium. By adopting a fluorescence analysis method, the high-sensitivity, high-selectivity, simple, rapid and accurate quantitative detection of the sugammadex can be realized according to the fluorescence enhancement efficiency of the sugammadex relative to the nano CDs after the sugammadex is added and a standard curve of the concentration of the sugammadex and the fluorescence enhancement efficiency. The fluorescence detection method provided by the invention is low in detection cost.

The utility model discloses a raw material batching device, which belongs to the technical field of fluorescence analysis, and comprises a support, wherein the support comprises a lower base, side plates installed at two ends of the lower base and an upper base installed on the side plates, a plurality of batching barrels are placed on the upper surface of the upper base, two clamping plates are arranged on opposite sides of the outer surface of the batching barrel, a plurality of clamping blocks are fixed on the side of the clamping plate close to the batching barrel, and a plurality of clamping grooves for the clamping blocks to be clamped are opened on the outer surface of the batching barrel; by arranging a plurality of batching barrels for use with label stickers, different raw materials can be calibrated, the batching barrels can be fixed with the clamping plates, the clamping blocks and the motion mechanism, no manual support is required for stability, the fixing effect is good, the pulley and the belt can be used to realize the rotation of a plurality of rotating shafts and stirring rods, and then a plurality of raw material samples can be stirred and batched, the batching effect is good, the operation is convenient, and the batching efficiency of multiple samples is improved.

The utility model discloses a rapid endotoxin detection device based on a recombinant C factor method, which comprises a shell with an opening on the front side; the kit is pushed into or pulled out of the shell through the opening by the bracket; the kit is placed on the bracket, a reaction cavity is formed in the kit, and a recombinant C factor reagent is stored in the reaction cavity; and the fluorescence analyzer is fixedly arranged above the kit in the shell. According to the endotoxin rapid detection device based on the recombinant C-factor method, recombinant C-factor reagents are added into the reagent storage area of the kit, the consistency of the recombinant C-factor reagents is high, batch difference does not exist, the accuracy is high when endotoxin detection is carried out, and the endotoxin rapid detection device is not limited by the yield of tachypleus amebocyte lysate. In the detection process, the recombinant C factor reagent is not exposed in the air, so that the influence of endotoxin in the air on the detection result is reduced.

The utility model discloses a modularized fluorescence analysis and detection device, which relates to the technical field of fluorescence analyzers and particularly comprises a first shell which is a control part, a display screen is arranged at the top of the first shell, a control mainboard is arranged in the first shell, and the modularized fluorescence analysis and detection device further comprises a second shell connected with the first shell; the second shell is a detection part, and a frame body for placing a sample cell is arranged in the second shell; a fluorescence receiving part is arranged at the top in the second shell, an irradiation part is arranged at the bottom in the second shell, the fluorescence receiving part is located above the sample cell, and the irradiation part is located below the sample cell. During use, the first electric actuator can be controlled to move by operating the keys, so that the positions of the fluorescence receiving part and the irradiation part are adjusted, and a proper distance is kept between the optical filter group at the bottom of the fluorescence receiving part and the sample cell; and the light beams emitted by the irradiation part can be driven by controlling the first electric actuator to be completely concentrated on the sample cell.

The invention provides an automatic calibration system for an X-ray fluorescence analyzer, which comprises an automatic calibration platform, a mechanical arm assembly and a control device, and is characterized in that the mechanical arm assembly is used for at least grabbing and placing a standard sample; the control device comprises a standard sample scheduling management module, a plurality of XRF process control modules and a mechanical arm control module, and the standard sample scheduling management module is used for maintaining standard sample state information, responding to a standard sample request and generating a standard sample scheduling task according to the standard sample state information; each XRF process control module is in communication connection with one X-ray fluorescence analyzer and is used for controlling the calibration process of the corresponding X-ray fluorescence analyzer and initiating a standard sample request to the standard sample scheduling management module; and the mechanical arm control module is in communication connection with the mechanical arm assembly and used for receiving the standard sample scheduling task and converting the standard sample scheduling task into an instruction sequence for controlling the mechanical arm assembly to act, and by means of the mode, multi-machine parallel calibration can be automatically achieved.

The invention belongs to the technical field of biological medicine detection, and particularly relates to an SERS / fluorescent bimodal composite probe, a preparation method and application thereof and a chiral glucose detection method, the composite probe is a gold nanorod modified by a tetraphenylethylene nuclear derivative, and the tetraphenylethylene nuclear derivative is a TPE nuclear molecular probe with AIE attributes; the tetraphenyl ethylene core derivative is prepared by reacting tetrabromo tetraphenyl ethylene with bis (pinacolato) diboron, oxidizing, and reacting with p-mercaptophenylboronic acid. Compared with the prior art, the method has the advantages that the limitations of long time consumption, insufficient sensitivity or specificity deficiency of the traditional chiral molecule identification method in the prior art are solved; and SERS (Surface Enhanced Raman Scattering) and fluorescence analysis are lack of methods and materials suitable for chiral molecule identification. According to the scheme, the composite nanoprobe can form a sandwich structure with molecules of an object to be detected, and rapid detection of chiral glucose is realized according to SERS intensity, fluorescence intensity and color change of the composite nanoprobe.

The invention relates to the technical field of oil-gas exploration, in particular to a method for measuring the oil content of an oil-based mud polluted rock core (rock debris). The method comprises the following steps: oil-based mud fluorescence quantification, sample pretreatment, ultrasonic oscillation extraction, extraction liquid fluorescence analysis, wellhead oil solvent fluorescence quantification and oil content calculation. The method comprises the following steps: collecting oil-based mud and wellhead oil on an exploration site, treating the oil-based mud and the wellhead oil with pesticide residue-grade dichloromethane, and determining respective fluorescence fingerprint spectrograms and characteristic peaks through three-dimensional fluorescence detection; the method comprises the following steps: grinding a rock core (rock debris) sample to 60-80 meshes, carrying out ultrasonic extraction to obtain an extract, detecting a spectrogram, identifying and deducting an oil-based mud pollution peak, and calculating the oil content by combining the correlation between the fluorescence intensity and concentration of wellhead oil. The method can effectively distinguish the oil-based mud from the oil content of the sample, realizes accurate detection of the oil content of the polluted sample, makes up for insufficient core data, is suitable for evaluation of conventional, unconventional, deep and ultra-deep oil and gas reservoirs, and provides reliable support for oil and gas exploration and the like.

A fluorescence analysis method for irradiating, with excitation light, a suspension in which a target substance is dispersed and analyzing measured intensity of fluorescence, the method comprising: acquiring a first intensity of fluorescence having a reference emission wavelength corresponding to the target substance and a second intensity of fluorescence having the reference emission wavelength or having an emission wavelength different from the reference emission wavelength, as measured by irradiating the suspension with excitation light having a reference excitation wavelength corresponding to the target substance; calculating representative values of the first intensity and the second intensity; and estimating the concentration of the target substance in the suspension on the basis of the representative values.

The invention belongs to a fluorescence analysis and detection method of p-nitrophenol, and particularly relates to a fluorescent carbon quantum dot probe for detecting p-nitrophenol as well as a preparation method and application of the fluorescent carbon quantum dot probe. The specific preparation method of the fluorescent carbon quantum dot probe comprises the following steps: taking aminopyrimidine as a precursor, mixing aminopyrimidine with water, and reacting at 160-200 DEG C to obtain the fluorescent carbon quantum dot probe with the surface rich in hydroxyl, amino and carboxyl groups. The fluorescent carbon quantum dot probe prepared by the invention has a strong fluorescent signal, selectively responds to p-nitrophenol, shows a wider linear detection range and high anti-interference capability, and is suitable for complex environmental pollutant detection.

The invention belongs to the technical field of copper electrolyte additive detection, and discloses a method for detecting the content of gelatin in a copper electrolyte based on an energy band regulation and control C3N4 fluorescent probe. Due to the fact that the intensity of fluorescence emitted by the C-C3N4 is not quenched due to existence of the cupric ions, when gelatin exists in the C-C3N4 solution, the quenching of the fluorescence of the C-C3N4 solution can be caused by existence of an extremely small amount of gelatin, and the concentration of the gelatin and the change value of the fluorescence intensity of the C-C3N4 are in a linear relation. C3N4 (C-C3N4) after energy band regulation and control is used as a fluorescent probe, a fluorescence analysis method is adopted, when a C-C3N4 solution is irradiated by light with the wavelength of 320 nm, an emission peak of the C-C3N4 can be seen at the position of 433 nm, and quantitative analysis of the gelatin in the copper electrolyte is achieved. The detection method is simple, convenient, efficient, high in detection sensitivity and low in detection limit, and has positive significance for effectively regulating and controlling the quality of the copper foil.

The preparation method comprises the following steps: adding a phosphor salt and urea into a carbon source, uniformly stirring, dissolving in water, carrying out a hydrothermal reaction at 180-200 DEG C, carrying out a series of purification operations such as suction filtration, high-speed centrifugation and dialysis, and obtaining the fluorescent carbon dots. Completely evaporating the solvent to dryness, replacing the solvent with equivalent absolute ethyl alcohol, fully dissolving by ultrasound, and filtering by using an organic filter head; the method solves the problems that the existing curcumin content detection cost is relatively high, the detection and analysis index error of a fluorescence analysis method is obvious, and the pretreatment process is relatively tedious. According to the method, after the curcumin and the fluorescent carbon dots have a synergistic effect, the characteristic half-peak width of a fluorescence emission spectrum of the coexisting solution, namely the light color purity in the coexisting solution, is regularly changed, and the content of the curcumin which promotes the change of the light color composition of the coexisting solution is measured according to the numerical value change condition of the half-peak width; and a curcumin quantitative analysis technology taking the high-performance fluorescent carbon dots as a detection medium is realized.

The utility model discloses an optical detection device of a fluorescence analyzer, comprising a base and a reference bracket fixed on the base, a camera and an annular light source are installed on the reference bracket, the camera and the annular light source are arranged along the vertical direction of the reference bracket, the annular light source is located below the camera, the lens end of the camera passes through the inner ring of the annular light source and faces the detection area, the annular light source comprises a light source housing and a plurality of LED light sources, the plurality of LED light sources are arranged at an angle on the shell wall of the light source housing facing the detection area, and the plurality of LED light sources are arranged along the circular track of the light source housing. The light spot area of ​​the utility model can cover the entire detection area of ​​the fluorescence analyzer, and can capture clear and recognizable images in a short exposure time, thereby improving the detection sensitivity and detection speed.

A current sensor for detecting an electrical current in a line (LTG) comprises means for optical excitation and fluorescence analysis of non-magnetic centers (NV) or paramagnetic centers (NV) in isotropic sensor elements (SE1, SE2). Pump radiation (LB) with a specific wavelength and intensity is used to generate fluorescent radiation (FL), the intensity and time delay of which are measured relative to the pump radiation. The current sensor generates a magnetic field through the current flow, which floods the non-magnetic centers (NV) with different magnetic flux densities (B1, B2). The current value (ILTG) is determined from the detected fluorescence intensities and delays. A computing module (CTR) enables the evaluation, storage, transmission, or output of the data.

The invention relates to a method for preparing a sample wafer for X fluorescence analysis of bentonite by using a melting method, which comprises the following steps: 1) adding a flux and a bentonite sample into a crucible, dropwise adding a boric acid-glycerol solution, and stirring until the mixture is muddy; (2) adding a liquid oxidant into the crucible, uniformly stirring, wiping the head of the glass rod with quantitative filter paper, and putting the wiped filter paper into the crucible; and 3) firing the crucible on a high-frequency sample melting machine until the solid in the crucible is molten into a liquid state, adding a release agent, slightly cooling, and demolding to obtain the sample for X fluorescence analysis. The method has the advantages that the operation is simple, the flaking rate is high, and prerequisite conditions are provided for quickly, accurately and reliably measuring the primary and secondary components of the bentonite. According to the method, the sample does not need to be fired at high temperature by a muffle furnace in advance, the platinum crucible does not need to be pretreated, the detection period is remarkably shortened, and the power consumption cost is reduced.

The invention discloses a power system equipment operation risk intelligent identification and early warning system. The system comprises an environmental parameter acquisition module, a passive ultraviolet fluorescence detection module, an active ultraviolet laser excitation module, a background fluorescence analysis and dynamic threshold calculation module, an abnormal signal confirmation module, an intelligent determination module and a closed loop verification and alarm module. Environment characteristic indexes are calculated by monitoring temperature, humidity and air pressure in real time; collecting and analyzing a natural ultraviolet fluorescence signal, actively emitting ultraviolet laser to excite environmental fluorescence response, and adaptively calculating a dynamic anomaly judgment threshold value; abnormal signals are accurately confirmed by utilizing multi-dimensional feature combination and feature space analysis, and corona discharge and line discharge are distinguished through intelligent judgment; and finally, carrying out automatic secondary verification positioning on the visible light image, and automatically generating a discharge early warning signal. The system has the characteristics of high sensitivity, strong interference resistance and high intelligent degree, and the discharge detection reliability is effectively improved.

1. Name of the Design Product: X-ray Fluorescence Analyzer (Model SDXRF1000). 2. Use of the Design Product: An X-ray fluorescence analysis instrument for non-ferrous metals, ferrous metals, ore dressing and smelting, geological industry, and coal industry. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective View 1.

The invention relates to a method for determining the amount of at least one printing bottom applied to at least one paper layer as a carrier material by means of X-ray fluorescence analysis, the method comprises the steps of: providing a plurality of reference samples by applying at least one printing bottoming material to at least one carrier material in respectively differently quantificationally defined amounts; recording at least one X-ray fluorescence spectrum of each of the reference samples with at least one X-ray fluorescence measurement head for the divalent cations to be detected respectively contained in the printing bottoming part; associating differently quantificationally defined amounts of the printed bottoming of the reference sample with the recorded X-ray fluorescence spectrum of the mentioned reference sample; and creating a calibration model for a relationship between the recorded X-ray fluorescence spectrum of the reference sample and the affiliated quantificationally defined amount of the printed bottoming; providing at least one sample to be measured by applying at least one layer consisting of at least one printing bottoming part to at least one carrier material; recording at least one X-ray fluorescence spectrum of the sample to be measured using at least one X-ray fluorescence measuring head; and determining a quantitative amount of the printed bottoming applied to the carrier material in the sample to be measured by comparing the X-ray fluorescence spectrum of the sample to be measured with a calibration model created from the reference sample.

A method for detecting an AFB1 content using fluorescence analysis based on optical sectioning is provided, which relates to the field of biological detection technologies. The method includes steps for detecting the AFB1 content by using a fluorescence probe combination, which includes fluorescence probes aptamer and PC-strand. The nucleotide sequence of the aptamer is shown as SEQ ID NO: 1, and a FAM group is labeled at a 3′ end of the nucleotide sequence shown as SEQ ID NO: 1. The nucleotide sequence of the PC-strand is shown as SEQ ID NO: 2, a TAMRA group is labeled at a 5′ end of the nucleotide sequence shown as SEQ ID NO: 2, and a PC group is labeled between 10th bp and 11th bp of the nucleotide sequence shown as SEQ ID NO: 2. The method can achieve photosensitive detection of AFB1, with high detection sensitivity and strong specificity.

Provided are a system for monitoring a hydroxyl radical scavenging index in water using a real-time multi-fluorescence analyzer and a parallel factor analysis apparatus and a method therefor, wherein the system monitors the hydroxyl radical scavenging index in water using the real-time multi-fluorescence analyzer and the parallel factor analysis apparatus, whereby it is possible to monitor the characteristics of an organic material in target water through a continuous flow analysis method without using an existing indicator material, rhodamine B. In addition, in a water treatment system having an advanced oxidation process (AOP) applied thereto in which ozone, ultraviolet rays, hydrogen peroxide, and the like are combined, it is possible to simply calculate the hydroxyl radical scavenging index in the target water through an organic material characteristic index for each component obtained by classifying the characteristic structure of the organic material in water using real-time fluorescence analysis by means of a parallel factor (PARAFAC) model. Accordingly, the amount of chemical injection and the amount of ultraviolet irradiation, which are process control variables, can be controlled, and under given operating variable conditions, the removal rate of a target material in water is predicted, whereby the system can also be used as a diagnostic tool for process evaluation in the advanced oxidation process. Furthermore, the system can provide operational convenience that enables process control while reducing the amount of power consumed in the advanced oxidation process even though the type of target material and the water quality characteristics of raw water change.

The utility model provides an eight-channel dry-type fluorescence analyzer which comprises a shell and a fluorescence detection head used for detecting a reagent card, the fluorescence detection head is movably arranged in the shell, a detection clamping plate is arranged in the shell, a clamping plate displacement assembly used for moving the detection clamping plate is movably arranged in the shell, and the detection clamping plate is movably arranged in the shell. Eight card supports are arranged on the detection card plate in parallel, each card support is movably connected to the detection card plate, eight microswitches which are in one-to-one correspondence with the card supports and are used for detecting whether reagent cards are inserted or not are arranged on the detection card support, and a sensor for positioning the positions of the reagent cards is arranged on the detection displacement assembly. An identification probe for scanning codes of the reagent card and a detection controller for receiving and analyzing data are arranged in the shell. The kit can be used for simultaneously detecting eight reagent cards, identifying codes of the reagent cards in batches, quickly recording detection data and improving the detection efficiency and the data analysis precision.

The present application relates to the field of molecular biology, in particular to a primer probe composition, kit and detection method for 23 human papilloma virus genotyping detection, wherein the hydrolysis probe and molecular beacon probe are respectively designed in two different regions of HPV gene sequence E6 and L1, and the fluorescence collection point in the amplification stage is set in the high-temperature denaturation stage, so that the detection in the amplification stage and the melting curve stage is independent and does not interfere with each other, thereby realizing the combined use of real-time fluorescent PCR amplification detection technology and melting curve technology; the technology improves the single-well detection target flux of fluorescent PCR analysis technology, reduces the detection cost, and provides a more optimal detection method for HPV multiplex fluorescent PCR genotyping detection.

The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.

The application provides an adenosine deaminase content detection method and application thereof. The adenosine deaminase content detection method comprises the following steps: using the intrinsic fluorescence of adenosine, using adenosine deaminase to hydrolyze adenosine, and detecting the content of adenosine deaminase by a fluorescence analysis method. Adenosine itself has strong fluorescence, and after being hydrolyzed by adenosine deaminase, the fluorescence of the obtained hydrolysis product is reduced, thereby causing the fluorescence intensity of the solution of the detection system to be reduced; the content of adenosine deaminase in the system is detected by the change of the fluorescence intensity before and after adenosine hydrolysis. The adenosine deaminase content detection method in the application is simple to operate, does not need large instruments and equipment, and is cheap and easy to obtain materials, can accurately and rapidly detect the content of adenosine deaminase in a sample to be detected, has good sensitivity and specificity, and has a relatively wide application prospect.

The present application relates to a fluorescence analysis method for rapidly determining the copper and zinc content and adhesion amount in brass-plated steel cords, and the fluorescence analysis method comprises the following steps: sample preparation, standard sample selection, instrument measurement conditions, working curve drawing, and accuracy and precision determination. The present invention arranges the samples of brass-plated steel cords or strands in an orderly manner and places them in an X-ray fluorescence analyzer to directly test the copper and zinc content, and the standard curve method to test the adhesion amount of the copper and zinc coating. The fluorescence analysis method of the present invention does not require stripping treatment, is simple to operate, and has a fast test speed; a shape correction function is introduced into the calibration curve, and the analysis results are accurate and reliable, providing a fast and accurate determination method for testing the element content and adhesion amount in the steel cord coating, solving the problem of testing the adhesion amount of steel cord samples, and meeting the fast and accurate testing needs of enterprises.

The invention relates to application of fluorescent ionic liquid in detection of nitrophenol isomers and a method, the fluorescent ionic liquid is (poly) 1, 3-bis (N-methylimidazolyl) propane 1, 4-naphthalic acid salt, and the structure of the compound is as shown in the following formula: # imgabs0 #. The preparation method comprises the following steps: firstly, sequentially adding 30mL of NaOH solution (3mol / L) and 9.47 g of 1, 4-naphthalic acid into a single-mouth bottle, carrying out reflux reaction at 30 DEG C for 24 hours, filtering, washing, and drying to obtain the fluorescent ionic liquid. The brown clear solution is the 1, 4-sodium naphthalate solution. Secondly, 1, 3-dibromopropane (8mL), N-methylimidazole (12mL) and acetone (50mL) are sequentially added into the three-neck flask; stirring and refluxing at 45 DEG C for 20 hours, washing with acetone for three times, 50mL each time, and carrying out suction filtration to obtain white solid powder; and finally, sequentially adding the prepared sodium 1, 4-naphthalate solution and 8.3 g of the white solid product obtained in the step 2 into a single-mouth flask, and stirring and reacting at 50 DEG C for 24 hours. And carrying out rotary evaporation, drying, absolute ethyl alcohol dilution, suction filtration and drying on the prepared solution to obtain the target product (poly) 1, 3-bis (N-methylimidazolyl) propane 1, 4-naphthalic acid salt. The method is simple and convenient to operate, has relatively high fluorescence intensity and sensitivity, and can be used for fluorescence analysis and analysis and identification of nitrophenol isomers.

This invention belongs to the field of compound technology and provides a DNA-intercalating fluorescent quenching compound, its preparation method, and its application. This invention provides a DNA-intercalating fluorescent quenching compound that exhibits absorption in the visible light region and certain DNA sequence selectivity. The DNA-intercalating fluorescent quenching compound provided by this invention can be further used to design fluorescence analysis systems for restriction endonuclease EcoRV and DNases. The DNA-intercalating quenching compound can achieve relevant fluorescence analysis by combining with fluorescent molecules in a covalent-free manner, reducing the need for chemical labeling steps, thereby improving efficiency and reducing detection costs, and showing good application prospects in related analyses.

The application discloses a method for analyzing silicon nitride manganese alloy by glass melting X-ray fluorescence spectrum, and comprises the following steps: step one, mixing a sample and an oxidizing agent and placing them in the middle of a platinum yellow crucible with a flux on the bottom; step two, placing the sample in an electric heating automatic melting furnace to prepare a to-be-detected glass disc by borate melting; step three, completely melting, automatically casting, cooling the sample disc, demolding and obtaining a glass disc for X fluorescence analysis; and step four, optimizing analysis parameters, establishing a working curve, performing sample analysis and obtaining the content of measured elements. The application relates to the technical field of metallurgical chemical analysis and detection, and particularly relates to a method for analyzing silicon nitride manganese alloy by glass melting X-ray fluorescence spectrum.