158 results about "Fluorometric Analysis" patented technology

The invention discloses a ratio-type fluorescence analysis method for determining sodium dehydroacetate, which comprises the following steps: under an alkaline condition, synthesizing tetrafluoroterephthalic acid oxide, namely a fluorescent substance TFAox, by oxidizing tetrafluoroterephthalic acid with hydrogen peroxide, and forming a ratio-type fluorescence probe by the fluorescent substance and rhodamine B, a ratio-type fluorescence analysis method of sodium dehydroacetate is constructed based on the ratio-type fluorescence probe, and the method can be successfully applied to determination of sodium dehydroacetate in a water sample and a bread sample. The ratio-dependent fluorescence detection method for determining sodium dehydroacetate provided by the invention has the characteristics of wide linear range, high detection sensitivity, no interference of coexisting substances on determination, good selectivity and the like.

The utility model discloses a rapid endotoxin detection device based on a recombinant C factor method, which comprises a shell with an opening on the front side; the kit is pushed into or pulled out of the shell through the opening by the bracket; the kit is placed on the bracket, a reaction cavity is formed in the kit, and a recombinant C factor reagent is stored in the reaction cavity; and the fluorescence analyzer is fixedly arranged above the kit in the shell. According to the endotoxin rapid detection device based on the recombinant C-factor method, recombinant C-factor reagents are added into the reagent storage area of the kit, the consistency of the recombinant C-factor reagents is high, batch difference does not exist, the accuracy is high when endotoxin detection is carried out, and the endotoxin rapid detection device is not limited by the yield of tachypleus amebocyte lysate. In the detection process, the recombinant C factor reagent is not exposed in the air, so that the influence of endotoxin in the air on the detection result is reduced.

The utility model discloses a modularized fluorescence analysis and detection device, which relates to the technical field of fluorescence analyzers and particularly comprises a first shell which is a control part, a display screen is arranged at the top of the first shell, a control mainboard is arranged in the first shell, and the modularized fluorescence analysis and detection device further comprises a second shell connected with the first shell; the second shell is a detection part, and a frame body for placing a sample cell is arranged in the second shell; a fluorescence receiving part is arranged at the top in the second shell, an irradiation part is arranged at the bottom in the second shell, the fluorescence receiving part is located above the sample cell, and the irradiation part is located below the sample cell. During use, the first electric actuator can be controlled to move by operating the keys, so that the positions of the fluorescence receiving part and the irradiation part are adjusted, and a proper distance is kept between the optical filter group at the bottom of the fluorescence receiving part and the sample cell; and the light beams emitted by the irradiation part can be driven by controlling the first electric actuator to be completely concentrated on the sample cell.

The invention provides an automatic calibration system for an X-ray fluorescence analyzer, which comprises an automatic calibration platform, a mechanical arm assembly and a control device, and is characterized in that the mechanical arm assembly is used for at least grabbing and placing a standard sample; the control device comprises a standard sample scheduling management module, a plurality of XRF process control modules and a mechanical arm control module, and the standard sample scheduling management module is used for maintaining standard sample state information, responding to a standard sample request and generating a standard sample scheduling task according to the standard sample state information; each XRF process control module is in communication connection with one X-ray fluorescence analyzer and is used for controlling the calibration process of the corresponding X-ray fluorescence analyzer and initiating a standard sample request to the standard sample scheduling management module; and the mechanical arm control module is in communication connection with the mechanical arm assembly and used for receiving the standard sample scheduling task and converting the standard sample scheduling task into an instruction sequence for controlling the mechanical arm assembly to act, and by means of the mode, multi-machine parallel calibration can be automatically achieved.

The invention belongs to the technical field of biological medicine detection, and particularly relates to an SERS / fluorescent bimodal composite probe, a preparation method and application thereof and a chiral glucose detection method, the composite probe is a gold nanorod modified by a tetraphenylethylene nuclear derivative, and the tetraphenylethylene nuclear derivative is a TPE nuclear molecular probe with AIE attributes; the tetraphenyl ethylene core derivative is prepared by reacting tetrabromo tetraphenyl ethylene with bis (pinacolato) diboron, oxidizing, and reacting with p-mercaptophenylboronic acid. Compared with the prior art, the method has the advantages that the limitations of long time consumption, insufficient sensitivity or specificity deficiency of the traditional chiral molecule identification method in the prior art are solved; and SERS (Surface Enhanced Raman Scattering) and fluorescence analysis are lack of methods and materials suitable for chiral molecule identification. According to the scheme, the composite nanoprobe can form a sandwich structure with molecules of an object to be detected, and rapid detection of chiral glucose is realized according to SERS intensity, fluorescence intensity and color change of the composite nanoprobe.

The invention relates to the technical field of oil-gas exploration, in particular to a method for measuring the oil content of an oil-based mud polluted rock core (rock debris). The method comprises the following steps: oil-based mud fluorescence quantification, sample pretreatment, ultrasonic oscillation extraction, extraction liquid fluorescence analysis, wellhead oil solvent fluorescence quantification and oil content calculation. The method comprises the following steps: collecting oil-based mud and wellhead oil on an exploration site, treating the oil-based mud and the wellhead oil with pesticide residue-grade dichloromethane, and determining respective fluorescence fingerprint spectrograms and characteristic peaks through three-dimensional fluorescence detection; the method comprises the following steps: grinding a rock core (rock debris) sample to 60-80 meshes, carrying out ultrasonic extraction to obtain an extract, detecting a spectrogram, identifying and deducting an oil-based mud pollution peak, and calculating the oil content by combining the correlation between the fluorescence intensity and concentration of wellhead oil. The method can effectively distinguish the oil-based mud from the oil content of the sample, realizes accurate detection of the oil content of the polluted sample, makes up for insufficient core data, is suitable for evaluation of conventional, unconventional, deep and ultra-deep oil and gas reservoirs, and provides reliable support for oil and gas exploration and the like.

A fluorescence analysis method for irradiating, with excitation light, a suspension in which a target substance is dispersed and analyzing measured intensity of fluorescence, the method comprising: acquiring a first intensity of fluorescence having a reference emission wavelength corresponding to the target substance and a second intensity of fluorescence having the reference emission wavelength or having an emission wavelength different from the reference emission wavelength, as measured by irradiating the suspension with excitation light having a reference excitation wavelength corresponding to the target substance; calculating representative values of the first intensity and the second intensity; and estimating the concentration of the target substance in the suspension on the basis of the representative values.

The invention belongs to a fluorescence analysis and detection method of p-nitrophenol, and particularly relates to a fluorescent carbon quantum dot probe for detecting p-nitrophenol as well as a preparation method and application of the fluorescent carbon quantum dot probe. The specific preparation method of the fluorescent carbon quantum dot probe comprises the following steps: taking aminopyrimidine as a precursor, mixing aminopyrimidine with water, and reacting at 160-200 DEG C to obtain the fluorescent carbon quantum dot probe with the surface rich in hydroxyl, amino and carboxyl groups. The fluorescent carbon quantum dot probe prepared by the invention has a strong fluorescent signal, selectively responds to p-nitrophenol, shows a wider linear detection range and high anti-interference capability, and is suitable for complex environmental pollutant detection.

A current sensor for detecting an electrical current in a line (LTG) comprises means for optical excitation and fluorescence analysis of non-magnetic centers (NV) or paramagnetic centers (NV) in isotropic sensor elements (SE1, SE2). Pump radiation (LB) with a specific wavelength and intensity is used to generate fluorescent radiation (FL), the intensity and time delay of which are measured relative to the pump radiation. The current sensor generates a magnetic field through the current flow, which floods the non-magnetic centers (NV) with different magnetic flux densities (B1, B2). The current value (ILTG) is determined from the detected fluorescence intensities and delays. A computing module (CTR) enables the evaluation, storage, transmission, or output of the data.

The invention discloses a power system equipment operation risk intelligent identification and early warning system. The system comprises an environmental parameter acquisition module, a passive ultraviolet fluorescence detection module, an active ultraviolet laser excitation module, a background fluorescence analysis and dynamic threshold calculation module, an abnormal signal confirmation module, an intelligent determination module and a closed loop verification and alarm module. Environment characteristic indexes are calculated by monitoring temperature, humidity and air pressure in real time; collecting and analyzing a natural ultraviolet fluorescence signal, actively emitting ultraviolet laser to excite environmental fluorescence response, and adaptively calculating a dynamic anomaly judgment threshold value; abnormal signals are accurately confirmed by utilizing multi-dimensional feature combination and feature space analysis, and corona discharge and line discharge are distinguished through intelligent judgment; and finally, carrying out automatic secondary verification positioning on the visible light image, and automatically generating a discharge early warning signal. The system has the characteristics of high sensitivity, strong interference resistance and high intelligent degree, and the discharge detection reliability is effectively improved.

1. Name of the Design Product: X-ray Fluorescence Analyzer (Model SDXRF1000). 2. Use of the Design Product: An X-ray fluorescence analysis instrument for non-ferrous metals, ferrous metals, ore dressing and smelting, geological industry, and coal industry. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective View 1.

The invention relates to a method for determining the amount of at least one printing bottom applied to at least one paper layer as a carrier material by means of X-ray fluorescence analysis, the method comprises the steps of: providing a plurality of reference samples by applying at least one printing bottoming material to at least one carrier material in respectively differently quantificationally defined amounts; recording at least one X-ray fluorescence spectrum of each of the reference samples with at least one X-ray fluorescence measurement head for the divalent cations to be detected respectively contained in the printing bottoming part; associating differently quantificationally defined amounts of the printed bottoming of the reference sample with the recorded X-ray fluorescence spectrum of the mentioned reference sample; and creating a calibration model for a relationship between the recorded X-ray fluorescence spectrum of the reference sample and the affiliated quantificationally defined amount of the printed bottoming; providing at least one sample to be measured by applying at least one layer consisting of at least one printing bottoming part to at least one carrier material; recording at least one X-ray fluorescence spectrum of the sample to be measured using at least one X-ray fluorescence measuring head; and determining a quantitative amount of the printed bottoming applied to the carrier material in the sample to be measured by comparing the X-ray fluorescence spectrum of the sample to be measured with a calibration model created from the reference sample.

The utility model provides an eight-channel dry-type fluorescence analyzer which comprises a shell and a fluorescence detection head used for detecting a reagent card, the fluorescence detection head is movably arranged in the shell, a detection clamping plate is arranged in the shell, a clamping plate displacement assembly used for moving the detection clamping plate is movably arranged in the shell, and the detection clamping plate is movably arranged in the shell. Eight card supports are arranged on the detection card plate in parallel, each card support is movably connected to the detection card plate, eight microswitches which are in one-to-one correspondence with the card supports and are used for detecting whether reagent cards are inserted or not are arranged on the detection card support, and a sensor for positioning the positions of the reagent cards is arranged on the detection displacement assembly. An identification probe for scanning codes of the reagent card and a detection controller for receiving and analyzing data are arranged in the shell. The kit can be used for simultaneously detecting eight reagent cards, identifying codes of the reagent cards in batches, quickly recording detection data and improving the detection efficiency and the data analysis precision.

The present application relates to the field of molecular biology, in particular to a primer probe composition, kit and detection method for 23 human papilloma virus genotyping detection, wherein the hydrolysis probe and molecular beacon probe are respectively designed in two different regions of HPV gene sequence E6 and L1, and the fluorescence collection point in the amplification stage is set in the high-temperature denaturation stage, so that the detection in the amplification stage and the melting curve stage is independent and does not interfere with each other, thereby realizing the combined use of real-time fluorescent PCR amplification detection technology and melting curve technology; the technology improves the single-well detection target flux of fluorescent PCR analysis technology, reduces the detection cost, and provides a more optimal detection method for HPV multiplex fluorescent PCR genotyping detection.

The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.

The application provides an adenosine deaminase content detection method and application thereof. The adenosine deaminase content detection method comprises the following steps: using the intrinsic fluorescence of adenosine, using adenosine deaminase to hydrolyze adenosine, and detecting the content of adenosine deaminase by a fluorescence analysis method. Adenosine itself has strong fluorescence, and after being hydrolyzed by adenosine deaminase, the fluorescence of the obtained hydrolysis product is reduced, thereby causing the fluorescence intensity of the solution of the detection system to be reduced; the content of adenosine deaminase in the system is detected by the change of the fluorescence intensity before and after adenosine hydrolysis. The adenosine deaminase content detection method in the application is simple to operate, does not need large instruments and equipment, and is cheap and easy to obtain materials, can accurately and rapidly detect the content of adenosine deaminase in a sample to be detected, has good sensitivity and specificity, and has a relatively wide application prospect.

This invention belongs to the field of compound technology and provides a DNA-intercalating fluorescent quenching compound, its preparation method, and its application. This invention provides a DNA-intercalating fluorescent quenching compound that exhibits absorption in the visible light region and certain DNA sequence selectivity. The DNA-intercalating fluorescent quenching compound provided by this invention can be further used to design fluorescence analysis systems for restriction endonuclease EcoRV and DNases. The DNA-intercalating quenching compound can achieve relevant fluorescence analysis by combining with fluorescent molecules in a covalent-free manner, reducing the need for chemical labeling steps, thereby improving efficiency and reducing detection costs, and showing good application prospects in related analyses.

The application discloses a method for analyzing silicon nitride manganese alloy by glass melting X-ray fluorescence spectrum, and comprises the following steps: step one, mixing a sample and an oxidizing agent and placing them in the middle of a platinum yellow crucible with a flux on the bottom; step two, placing the sample in an electric heating automatic melting furnace to prepare a to-be-detected glass disc by borate melting; step three, completely melting, automatically casting, cooling the sample disc, demolding and obtaining a glass disc for X fluorescence analysis; and step four, optimizing analysis parameters, establishing a working curve, performing sample analysis and obtaining the content of measured elements. The application relates to the technical field of metallurgical chemical analysis and detection, and particularly relates to a method for analyzing silicon nitride manganese alloy by glass melting X-ray fluorescence spectrum.

The application discloses a fluorescence analysis system for specific response to p-nitrophenol based on gold nanoclusters AuNCs@CFP, 200 muL of gold nanoclusters AuNCs@CFP, 500 muL of PB buffer solution with pH=10 and to-be-detected p-nitrophenol are diluted to 4 mL, the fluorescence intensity of the mixture is determined at an excitation wavelength of 334 nm after reaction at 45 DEG C for 50 min, the concentration of the to-be-detected p-nitrophenol is calculated according to the determined fluorescence intensity and a regression equation, the linear concentration range of the p-nitrophenol is 1.25-40 muM, the regression equation is F0 / F i =0.0258C+1.0003, F0 and F i are the fluorescence intensities of the mixture before and after the addition of the p-nitrophenol respectively, the correlation coefficient R 2 =0.9922, the detection limit LOD is 1.92 muM, the concentration of the p-nitrophenol is 25 muM, the relative standard deviation RSD is 1.13% in 11 repeated determinations. The preparation method of the gold nanoclusters AuNCs@CFP in the application is simple and easy to synthesize, and the fluorescence performance of the gold nanoclusters AuNCs@CFP is excellent.

The invention discloses preparation of a B and N co-doped dual-emission ratio type fluorescent carbon quantum dot fluorescent probe and application of the B and N co-doped dual-emission ratio type fluorescent carbon quantum dot fluorescent probe in atrazine detection, and belongs to the technical field of nano functional materials and fluorescence analysis and detection. When the fluorescent carbon quantum dot fluorescent probe is prepared, the optimal reaction temperature is 160 DEG C, the optimal heating time is 5 hours, the optimal raw material ratio of 3-CPBA to PPD is 2: 1, and the optimal pH is 8. The concentration range of the detected atrazine is 0.15-600 [mu] g / L, the I660 / I380 ratio and the concentration of the atrazine show a good linear relationship, the linear fitting equation is y = 0.004 [atrazine] + 0.29, and the detection limit is 0.187 [mu] g / L. 3.5 min is selected as the detection time, so that both efficiency and accuracy can be considered. The probe has a specific recognition capability on atrazine in actual sample detection, and has a good detection effect. The method has great significance in the field of atrazine detection.

The present invention relates to a qPCR microfluidic chip card and a real-time fluorescence PCR analyzer, comprising: a microfluidic chip base body; a sample pretreatment section and a reaction chamber, the reaction chamber communicating with the mixing section; a first exhaust air flow channel used to connect the reaction chamber to the outside environment to discharge gases from the reaction chamber; and a first air-permeable water separator located between the first exhaust air flow channel and the reaction chamber.wherein the main body of the microfluidic chip comprises a blocking region, and wherein at least a part of the first exhaust flow channel is located in the blocking region, and wherein the at least one part of the first exhaust flow channel located in the blocking region can be sealed with a heating and / or extrusion deformation process, and wherein the blocking region is heated and / or extruded so that the first exhaust flow channel located in the blocking region is blocked, and during amplification, the aerosol generated by the heating cannot enter the external environment through the first exhaust flow channel to prevent the aerosol from contaminating the external environment, thus reducing the hidden danger to biological safety during detection.

The invention belongs to the field of antibiotic detection, and relates to detection of norfloxacin (NOR). The invention provides a Luminol-Eu-NCDs ratiometric fluorescent probe as well as a preparation method and application of the Luminol-Eu-NCDs ratiometric fluorescent probe. According to the application, a novel ratiometric fluorescence sensor is constructed through covalent linkage of Luminol-Eu self-assembled and synthesized by AIE and NCDs derived from Listeria monocytogenes. The Luminol-Eu NPs with the AIE effect overcomes the fluorescence quenching characteristic of a traditional fluorophore in a high concentration or aggregation state, so that the reference fluorescence brightness of fluorescence analysis is greatly improved. In addition, due to the introduction of the Luminol-Eu NPs, the sensitivity and the accuracy of NOR detection can also be improved. On the basis, a simple ratio fluorescence sensor with high selectivity is developed, and the ratio fluorescence sensor is used for detecting NOR residues in a milk sample.

The invention discloses a glass fiber raw material-oriented robot cooperation online XRF analysis system, which comprises two multi-channel quantitative sampling and sample preparation units, a multifunctional measurement unit and a central control system, and two cooperation robot units are arranged in an open operation area between the two multi-channel quantitative sampling and sample preparation units; the central control system is electrically connected with the two multi-channel quantitative sampling and sample preparation units, the multifunctional measuring unit and the two collaborative robot units, the material quantity is accurately controlled through a quantitative feeding mechanism of a double-stage gate plate matched material level sensor, powder is pressed into a sample cake with uniform density through a constant-pressure compaction assembly, and the sample cake is pressed into a sample. Meanwhile, the measuring station enables the sample cup to be tightly attached to the window of the detection equipment through an exclusive jacking mechanism, so that the X fluorescence analyzer is accurately matched with the moisture measuring instrument, and the measuring precision is comprehensively improved from sampling quantification, sample preparation form to the detection process.

Chip laboratory cartridge (1), preferably for single use, in particular for a modular medical analysis system (20), comprising a sample chamber (2), wherein a fluorescent dye (4) is contained in the sample chamber (2) and wherein the fluorescent dye (4) is adhesive to a specific target molecule.

The invention discloses a phosphorite grade sorting method and equipment based on an FPGA (Field Programmable Gate Array) and an ARM (Advanced RISC Machines) architecture, and relates to the technical field of phosphorite sorting. The method comprises the steps that a regression model is constructed, ore phosphorus grade data of a known grade is collected through a handheld X-ray fluorescence analyzer to serve as sample data, and model training is achieved; the method comprises the following steps: acquiring hyperspectral data and spectrums of ores on a transmission line in real time through a hyperspectral camera, dividing the hyperspectral data and spectrums into a plurality of narrow bands, and converting a signal of each narrow band into band reflectivity data; according to the method, hyperspectral data are converted into pseudo-color data, a plurality of wave band reflectivity data are established into multi-channel image data, grade regression is performed on each pixel point through a regression model, so that the ore surface phosphorus distribution condition is calculated, and grade sorting is realized. According to the method, the hyperspectral data of the ore is collected in real time through the hyperspectral camera, grade calculation and sorting are carried out through the constructed regression model, and the problems that existing phosphorite grade sorting is low in double speed and low in accuracy are solved.

The invention belongs to the technical field of fluorescence sensing, and particularly relates to a fluorescence analysis method which comprises the following steps: mixing a solution to be detected with chromium selenide quantum dots, and detecting the fluorescence intensity of a system to obtain the content of sodium tripolyphosphate in the solution to be detected. According to the present invention, it is found that sodium tripolyphosphate can selectively quench the fluorescence of chromium selenide quantum dots, such that the chromium selenide quantum dot-based sodium tripolyphosphate fluorescence analysis method is provided so as to meet the requirement of efficient detection of sodium tripolyphosphate.

The application discloses a preparation and application of a fluorescent probe for specifically detecting human serum albumin, which can be used as a high-sensitivity and high-selectivity HSA fluorescent analysis method. The self-assembled nano fluorescent probe has the advantages of excellent selectivity, good anti-interference ability, rapid response, high sensitivity, good biocompatibility, strong stability, simple preparation, low synthesis cost and the like. The nanoparticles constructed by quinoline hydrazine Schiff base and HSA can be used as a visual carrier to carry platinum drugs. The nanoparticles have the advantages of emitting fluorescence under light excitation, thereby playing a tracing role on the nanoparticles. After the nanoparticles are dissociated, the fluorescence disappears, so that the release of the platinum drugs in a biological system can be monitored.

The invention relates to a filter device comprising a first filter carrier (10) with x first optical filters (11, 12) and a second filter carrier (20) with y second optical filters (21 - 23), wherein x and y each independently have a value of at least 2, and wherein the filter carriers (10, 20) are arranged to arrange one filter (11, 12, 21 - 23) of each filter carrier (10, 20) in a beam path of the filter device.Each first optical filter (11, 12) allows light of a respective first wavelength band (71, 72) to pass through, and each second optical filter (21-23) allows light of x respective second wavelength bands (81-86) to pass through, wherein the first wavelength bands (71, 72) do not overlap, the second wavelength bands (81-86) do not overlap, and each first wavelength band (71, 72) contains one second wavelength band (81-86) of each of the second optical filters (21-23). ​​An optical analysis device comprises a light source, a sample carrier, and an optical sensor. The filter device is arranged in a beam path between the light source and the sample carrier and / or in a beam path between the sample carrier and the optical sensor.In a method for operating the optical analysis device, a sample is arranged on the sample carrier, excited to fluorescence by means of the light source, and light emitted by the sample is detected by the optical sensor, whereby a fluorescence analysis of the sample is carried out in x · y optical channels.

The present invention relates to a CRISPR-Cas12a-based exosomal miRNA one-pot analysis method and device using an hourglass structure. The present invention provides an miRNA analysis method and device which can continuously perform the isolation and amplification of exosomes and the analysis of CRISPR Cas signals in a single reaction vessel by using multifunctional nanoparticles and an hourglass structure. The analysis method according to the present invention is characterized by: using an exosome isolation method using magnetic nanoparticles and an immune response without external equipment such as a centrifuge, thereby enabling selective isolation of only human cell-derived exosomes in a simple manner and at low cost; amplifying miRNA with CRISPR RNA (crRNA) instead of an aptamer, thus simplifying the amplification process; and analyzing a signal generated when a chromogenic enzyme immobilized via DNA is cleaved by Cas protein, thereby enabling analysis with the naked eye without separate fluorescence analysis equipment.

The present invention relates to a fluorescent nanoparticle, a fluorescent probe, a preparation method and an application thereof. The fluorescent nanoparticle is an SrS nanoparticle doped with Pr, Sm and Mn. The fluorescent probe comprises mesoporous silica loaded with the fluorescent nanoparticle, a DNAzyme complex coupled to the surface of the mesoporous silica, and a shell located on the outer layer of the DNAzyme complex. In the presence of near-infrared light and a specific nucleic acid fragment, the present invention activates the fluorescent probe system and releases the cyanine dye FD-1080 in the DNAzyme complex structure, and monitors the near-infrared second-region fluorescence emission of FD-1080 to realize the analysis of the target fragment. The present invention not only broadens the selectable fluorescent nanomaterials for biological imaging, but also contributes to the development of near-infrared light-controlled biochemical sensors and near-infrared light-controlled drug delivery carriers, and promotes the application of fluorescence analysis technology and light-controlled nanomachines in the field of biomedical detection.