353 results about "Fluorometric Analysis" patented technology

The present invention provides instruments for analyzing a multiplicity of fluorescent dyes using a multiplicity of amplifying photodetectors, methods for using the instruments, methods for setting the instrument parameters, and methods for resetting the instrument parameters following a changed in photodetector amplification. The present invention is particularly applicable in the field of flow cytometry.

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.

The invention relates to a method based on near infrared fluorescence molecules and nano particle marks as well as an immunochromatography quantitative detection reagent based on near infrared fluorescence nanoparticles. According to the invention, infrared fluorescence is connected with nanoparticles to prepare an immunochromatography test strip based on the near infrared fluorescence nanoparticles. During the detection, an infrared light scanner is utilized, and a quality control line and a sample line are respectively scanned by utilizing near infrared light; and after the fluorescence intensity of a detection line is rectified by utilizing the fluorescence intensity of a quality control line, and a standard curve in a fluorescence analyzer is taken in, the concentration of a to-be-detected matter in a sample can be analyzed and detected. Compared with the direct connection of the near infrared fluorescence molecules and detecting molecules, the near infrared fluorescence nanoparticle immunochromatography improves the detection flexibility obviously, and the lowers the background fluorescence intensity. The marking method and the reagent can be applied to microorganism detection, food safety detection, poison detection as well as rapid dangerous chemical product detection.

An X-ray multiple spectroscopic analyzer includes an X-ray source, an optical system inputting X-rays to a single-crystal sample, a sample stage supporting the single-crystal sample, an X-ray diffraction detector, a rotation driving system that changes the angle of the X-ray diffraction detector, an X-ray diffraction measurement data storage unit, a structural analysis data analyzing unit, an energy-dispersive X-ray fluorescence detector, an X-ray fluorescence measurement data storage unit, an X-ray fluorescence analyzing unit, an X-ray fluorescence analysis data storage unit, and X-ray fluorescence analysis data acquiring unit. The structural analysis data analyzing unit analyzes the data of the crystal structure further on the basis of the analysis data of the fluorescent X-rays output from the X-ray fluorescence analysis data acquiring unit.

The invention relates to an immunochromatographic quantitative test reagent based on a near-infrared fluorescent marker. A near-infrared fluorescent molecule is adopted as a marker, and the immunochromatographic technology is adopted to prepare a near-infrared fluorescent immunochromatographic test strip. In the test process, a conventional near-infrared test instrument is used for respectively scanning a quality control line and a sample line through near-infrared rays, and after being corrected by utilizing the fluorescent strength of the quality control line, the fluorescent strength of a detection line is substituted in a standard curved line in a fluorescent analysis instrument, so that the concentration of an object to be tested in a test specimen can be analyzed. The reagent can be applied to the detection of microorganism, the food safety detection, the drug detection and the quick detection of dangerous chemicals. The immunochromatographic quantitative test reagent has characteristics of high sensitivity, accuracy in quantization and convenience in operation.

Provided are a method and an apparatus for easily identifying and detecting fluorescent material types captured in respective reaction regions of a substrate, particularly, a method and an apparatus for identifying and measuring the fluorescence intensities of a plurality of fluorescent materials using a small pixel count. The fluorescence intensities of four or more types of fluorescent materials are divided by a dividing section at ratios different at least for each fluorescence maximum wavelength range, and are detected by at least one detector including pixels for detecting the light fluxes divided at the different ratios. The type of the fluorescent material is determined on the basis of the ratio of the detected fluorescence intensity of the same detection portion, and the fluorescence intensity is measured.

In the present invention, a specimen is made to flow on a metal film on a prism, and excitation light (α) is emitted in a predetermined direction. By changing the position of a reflective member that reflects the excitation light (α), and adjusting the orientation of a reflective surface of the reflective member, the incident angle (θ) is changed while maintaining a state in which the excitation light (α) that enters the prism is reflected at a specific position on the metal film. The intensity of light to be generated on the metal film is measured, and the reflective member is positioned to match the position of the reflective member and the orientation of the reflective surface when a maximum amount of light is measured.

The invention discloses a fluorescence microballoon immunochromatography testing card for quantitatively testing HIV and preparation method. The testing card comprises A and B test paper, a sample cushion, a glass fibrous membrane, a nitrocellulose membrane and drinking paper, wherein, the nitrocellulose membrane is thereon fixed with a test line and a quality control line for realizing the simultaneous test of HIV antibody and HIV p24 antigen. The invention takes nucleocapsid dual-structural light-emitting nano-particles compounded by silicon dioxide and fluorescent substance as marks and adopts immunochromatographic technique to realize quantitative immunoassay of HIV. In the testing process, fluorescence microballoon excitation light source is adopted to carry out excitation, after the emitted fluorescence passes through an optical filter device, all emission spectra are collected, aggregated and multiplied by CCD scanning technique or optical fiber technique, and are then converted into numerical signals, the concentration of the substance to be measured is automatically calculated by using the built-in analysis software in a fluorescence analyzer. The invention has the advantages of high sensitivity, precise quota, fast detection, convenient operation, and economy and practicality.

The invention discloses a fluorescence analysis method (/b) for determining glucose by employing (b) nanometer copper oxide as simulated peroxide. The fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide is characterized by comprising the following steps: firstly, mixing glucose, glucose oxidase and phosphate buffer in a warm bath, and then adding the phosphate buffer; continuing to carry out warm bath on terephthalic acid and the nanometer copper oxide, wherein the fluorescence excitation wavelength and the emission wavelength of a reaction product of the mixing solution are 315nm and 421nm, the linear range of glucose content measurement is 3-120mumol/L, and the detection limit is 1.94mumol/L. Therefore, the method can be applied to determination of blood sugar concentration.

The invention relates to a full-elemental analysis method for a stainless steel sample, and belongs to the field of metallurgical analysis. According to the method, a direct reading spectrometry method and a X-ray fluorescence analysis method are respectively adopted to analyze elements of C, Si, Mn, P, S, Cu, Al, Mo, V, Ti, Co, As, Sn and Pb; the analysis result of the direct reading spectrometry method and the analysis result of the X-ray fluorescence analysis method are subjected to a correlation treatment; concentration input ports corresponding to the elements of C, Si, Mn, P, S, Cu, Al,Mo, V, Ti, Co, As, Sn and Pb are added to a X-ray fluorescence analyzer; a direct reading spectrometer is adopted to analyze the elements of C, Si, Mn, P, S, Cu, Al, Mo, V, Ti, Co, As, Sn and Pb; theX-ray fluorescence analyzer is adopted to analyze the elements of Cr, Ni and Fe; the direct reading spectrometer transmits the analysis result to the X-ray fluorescence analyzer by network to performinterference correction calculations of the elements of Cr, Ni and Fe; the full-elemental analysis result of the stainless steel sample is reported after all the data are merged. With the present invention, the full elements of the stainless steel sample can be rapidly analyzed, and the analysis time is 5 minutes.

An X-ray fluorescence analysis method according to an FP method uses a predefined theoretical intensity formula in a standard sample theoretical intensity calculation step for obtaining a sensitivity constant and in an unknown sample theoretical intensity calculation step during iterative calculation. In the formula, only in an absorption term relating to absorption of X-rays, a mass fraction of each component is normalized so that a sum of the mass fractions of all components becomes 1.

The invention discloses a hydrogel microsphere fluorescence sensor as well as a preparation method and application thereof. By use of the advantages of a hydrogel material, the hydrogel material is applied to wrapping a fluorescence probe; the hydrogel is fully mixed with the fluorescence probe, and the mixture is directly processed into a fluorescence sensor system which has a microsphere structure and is applicable to fluorometric analysis and detection by virtue of an ultraviolet polymerization reaction; successfully cured hydrogel micropheres have favorable flexible structures and can not release hydrogel monomers without influencing detection; due to a net-shaped structure, the wrapped fluorescence probe is quite stably dispersed in the hydrogel with out leaking through the micropheres; detection substances can freely enter into or move out of the whole microsphere fluorescence sensor system; in combination with a fluorescence microscope, the hydrogel microsphere fluorescence sensor is capable of quite conveniently analyzing and detecting ions, molecules and other relevant substances; the hydrogel microsphere fluorescence sensor is a microsphere which is formed by wrapping the fluorescence probe with hydrogel and has a diameter of 300-500 mu m.

The invention relates to fields of biochemical analysis, and concretely relates to a homogeneous time-resolved fluorescence analysis method of alpha-fetalprotein. The method comprises the following steps: 1, preparing a quantitative standard curve: incubating an HFRFA energy receptor, an HFRFA energy donor and an alpha-fetalprotein standard substance together, and carrying out time-resolved fluorescence detection and analysis to obtain the standard curve between the concentration of the alpha-fetalprotein and the fluorescence value; and 2, detecting a sample: incubating the HFRFA energy receptor, the HFRFA energy donor and the alpha-fetalprotein sample to be detected together, carrying out time-resolved fluorescence detection and analysis, comparing the fluorescence value obtained through detecting the sample with the standard curve obtained in step 1, and calculating to obtain the concentration of the alpha-fetalprotein sample to be detected. The method of the invention, which has the advantages of strong specificity and high detection sensitivity, has important meanings in the biochemical analysis of clinical molecular diagnosis and food detection.

Described herein are systems for analysis of biopolymers and complexes containing biopolymers based on optical measurement of ion flux through pores. Also described are methods of using such devices for analysis of biopolymers and complexes containing biopolymers, including methods of determining the nucleotide sequences of polynucleotides.

The invention provides a glass reagent for preparing glass fuse pieces used for metallurgical flux X-ray fluorescence analysis, consisting of anhydrous lithium tetraborate or mixture of anhydrous lithium tetraborate and lithium metaborate, and boric acid. A method for preparing the glass fuse pieces includes: firstly adding the anhydrous lithium tetraborate or the mixture of anhydrous lithium tetraborate and lithium metaborate in the metallurgical flux, then fusing at a high temperature for 12-15mins in a platinum yellow pot, preparing the glass fuse pieces after cooling; or fully mixing the boric acid with the anhydrous lithium tetraborate or the mixture of the anhydrous lithium tetraborate and lithium metaborate in advance, preparing the fast fused glass reagent and then mixing with the metallurgical flux, then fusing at the high temperature for 12-15mins in the platinum yellow pot, and preparing the glass fuse pieces after cooling. The glass reagent has accurate and stable analysis results, and greatly shortens the analysis period of the metallurgical fusing agent.

The invention discloses an X-ray detection method for a fluorescence analyzer. The method comprises the following steps of: separating the characteristic X-ray of an object to be detected by utilizing the Bragg's law; detecting the separated characteristic X-ray by an X-ray detector to obtain spectroscopic data; processing the spectroscopic data of the separated characteristic X-ray by an energy dispersion method; detecting the characteristic X-ray of all elements before light splitting by singly using an X-ray detector with high resolution, and carrying out spectrum peak calculation by the energy dispersion method. Grade calculation is carried out on data processed by using two methods, thereby the grade analysis precision can be improved compared with that obtained by singly using one analysis method. The invention is particularly suitable for the condition that the physical property of the object to be detected has greater change and the conditions of low-grade measurement and nonmetal element measurement.

The invention belongs to the technical fields of material preparation, analysis and detection, and discloses a preparation method of a rare-earth fluorescent molecularly imprinted membrane and an application in detection of cyfluthrin. According to the technical scheme disclosed by the invention, the method comprises the following steps: preparing an Eu (III) rare-earth complex by a solution method; wrapping the rare-earth complex with a silicon-based surface; adopting a surface molecular imprinting technology; and with cyfluthrin as a template, methacrylic acid (MAA) as a functional monomer and EGDMA as a cross-linking agent and azobisisobutyronitrile (AIBN) as an initiator, preparing a rare-earth fluorescent molecularly imprinted probe. Trace cyfluthrin in a water sample is detected by a fluorescence analysis method; the related coefficient R2 is 0.99269; and the result proves that the rare-earth fluorescent molecularly imprinted probe obtained by the method has excellent recognition performance and extremely high sensitivity on cyfluthrin molecules.

The invention discloses a portable fluorescence analyzer, which comprises a test strip compartment for inserting a rapid fluorescence chromatography diagnostic test strip; Fluorescence chromatography quickly diagnoses the fluorescent substances on the test strips to generate fluorescent emission light; the camera is located at the entrance above the collection channel on the excitation light source to collect the fluorescence emission light of the fluorescent test strips; the communication module is used to send the images collected by the camera ; The processing module is used to control the excitation light source, the camera and the communication module to coordinate their work.

A fiber spectroscopic probe that can be mounted directly above the objective lens of a standard microscope to add a spectroscopic function to the microscope. The constructed microscope with fiber spectroscopic probe is suitable for micro-sampling, Raman analysis, as well as fluorescence analysis and can be easily reconfigured for different excitation/detection wavelengths. The fiber spectroscopic probe only consists of a minimum number of optical components and is compact enough to induce minimum alteration to the optical path of the microscope.

A novel phenazine imidazo phenylboronic acid fluorescence sugar probe adopts phenylboronic acid to modify phenazine imidazo compounds, and the structure of the compound is 2-(4'-phenylboronic acid)-1H-phenazine[2,3-d]imidazo. The fluorescence sugar probe can be used for buffering the detection of sugar in aqueous solution, that is, boric acid reacts with sugar molecules when being added with sugar so as to generate boric acid lactone, and electron migration in a system is led to be changed, so that the fluorescence intensity of a phenazine imidazo system is improved. The novel phenazine imidazo phenylboronic acid fluorescence sugar probe has the advantages as follows: excitation wavelength can be within a visible region (ranging from 350 to 770nm), the emission region is positioned in long wavelength, and larger Stokes shift can be achieved; higher saccharimetry selectivity and sensitivity can be achieved; the novel phenazine imidazo phenylboronic acid fluorescence sugar probe is suitable for the real-time fluorometric analysis of blood sugar in the system; and the stability is good, and the novel phenazine imidazo phenylboronic acid fluorescence sugar probe can be used and stored for a long time. The fluorescence sugar probe provides a better research method for the research on biological signal channels relative to biological phenylboronic acid as well as significance and diagnosis of the phenylboronic acid in certain disease or in vivo process.

The present invention includes optical path, circuit, computer and operation platform. The optical path includes light source, optical filters, objective, half-reflecting mirror, focusing lens, pinhole and photomultipler tube. The circuit includes the part for data collection of photomultipler tube, A/D converter, high voltage relay, high voltage output controller and high voltage output detection unit. The computer is connected to the circuit via communicator. The present invention features the computer with CE WINDOWS application software for parameter setting, peak detection and data processing.