66 results about "Fluorometric Analysis" patented technology

The utility model discloses a rapid endotoxin detection device based on a recombinant C factor method, which comprises a shell with an opening on the front side; the kit is pushed into or pulled out of the shell through the opening by the bracket; the kit is placed on the bracket, a reaction cavity is formed in the kit, and a recombinant C factor reagent is stored in the reaction cavity; and the fluorescence analyzer is fixedly arranged above the kit in the shell. According to the endotoxin rapid detection device based on the recombinant C-factor method, recombinant C-factor reagents are added into the reagent storage area of the kit, the consistency of the recombinant C-factor reagents is high, batch difference does not exist, the accuracy is high when endotoxin detection is carried out, and the endotoxin rapid detection device is not limited by the yield of tachypleus amebocyte lysate. In the detection process, the recombinant C factor reagent is not exposed in the air, so that the influence of endotoxin in the air on the detection result is reduced.

The present application relates to the field of molecular biology, in particular to a primer probe composition, kit and detection method for 23 human papilloma virus genotyping detection, wherein the hydrolysis probe and molecular beacon probe are respectively designed in two different regions of HPV gene sequence E6 and L1, and the fluorescence collection point in the amplification stage is set in the high-temperature denaturation stage, so that the detection in the amplification stage and the melting curve stage is independent and does not interfere with each other, thereby realizing the combined use of real-time fluorescent PCR amplification detection technology and melting curve technology; the technology improves the single-well detection target flux of fluorescent PCR analysis technology, reduces the detection cost, and provides a more optimal detection method for HPV multiplex fluorescent PCR genotyping detection.

The application provides an adenosine deaminase content detection method and application thereof. The adenosine deaminase content detection method comprises the following steps: using the intrinsic fluorescence of adenosine, using adenosine deaminase to hydrolyze adenosine, and detecting the content of adenosine deaminase by a fluorescence analysis method. Adenosine itself has strong fluorescence, and after being hydrolyzed by adenosine deaminase, the fluorescence of the obtained hydrolysis product is reduced, thereby causing the fluorescence intensity of the solution of the detection system to be reduced; the content of adenosine deaminase in the system is detected by the change of the fluorescence intensity before and after adenosine hydrolysis. The adenosine deaminase content detection method in the application is simple to operate, does not need large instruments and equipment, and is cheap and easy to obtain materials, can accurately and rapidly detect the content of adenosine deaminase in a sample to be detected, has good sensitivity and specificity, and has a relatively wide application prospect.

The application discloses a fluorescence analysis system for specific response to p-nitrophenol based on gold nanoclusters AuNCs@CFP, 200 muL of gold nanoclusters AuNCs@CFP, 500 muL of PB buffer solution with pH=10 and to-be-detected p-nitrophenol are diluted to 4 mL, the fluorescence intensity of the mixture is determined at an excitation wavelength of 334 nm after reaction at 45 DEG C for 50 min, the concentration of the to-be-detected p-nitrophenol is calculated according to the determined fluorescence intensity and a regression equation, the linear concentration range of the p-nitrophenol is 1.25-40 muM, the regression equation is F0 / F i =0.0258C+1.0003, F0 and F i are the fluorescence intensities of the mixture before and after the addition of the p-nitrophenol respectively, the correlation coefficient R 2 =0.9922, the detection limit LOD is 1.92 muM, the concentration of the p-nitrophenol is 25 muM, the relative standard deviation RSD is 1.13% in 11 repeated determinations. The preparation method of the gold nanoclusters AuNCs@CFP in the application is simple and easy to synthesize, and the fluorescence performance of the gold nanoclusters AuNCs@CFP is excellent.

The invention discloses preparation of a B and N co-doped dual-emission ratio type fluorescent carbon quantum dot fluorescent probe and application of the B and N co-doped dual-emission ratio type fluorescent carbon quantum dot fluorescent probe in atrazine detection, and belongs to the technical field of nano functional materials and fluorescence analysis and detection. When the fluorescent carbon quantum dot fluorescent probe is prepared, the optimal reaction temperature is 160 DEG C, the optimal heating time is 5 hours, the optimal raw material ratio of 3-CPBA to PPD is 2: 1, and the optimal pH is 8. The concentration range of the detected atrazine is 0.15-600 [mu] g / L, the I660 / I380 ratio and the concentration of the atrazine show a good linear relationship, the linear fitting equation is y = 0.004 [atrazine] + 0.29, and the detection limit is 0.187 [mu] g / L. 3.5 min is selected as the detection time, so that both efficiency and accuracy can be considered. The probe has a specific recognition capability on atrazine in actual sample detection, and has a good detection effect. The method has great significance in the field of atrazine detection.

The invention discloses a glass fiber raw material-oriented robot cooperation online XRF analysis system, which comprises two multi-channel quantitative sampling and sample preparation units, a multifunctional measurement unit and a central control system, and two cooperation robot units are arranged in an open operation area between the two multi-channel quantitative sampling and sample preparation units; the central control system is electrically connected with the two multi-channel quantitative sampling and sample preparation units, the multifunctional measuring unit and the two collaborative robot units, the material quantity is accurately controlled through a quantitative feeding mechanism of a double-stage gate plate matched material level sensor, powder is pressed into a sample cake with uniform density through a constant-pressure compaction assembly, and the sample cake is pressed into a sample. Meanwhile, the measuring station enables the sample cup to be tightly attached to the window of the detection equipment through an exclusive jacking mechanism, so that the X fluorescence analyzer is accurately matched with the moisture measuring instrument, and the measuring precision is comprehensively improved from sampling quantification, sample preparation form to the detection process.

Chip laboratory cartridge (1), preferably for single use, in particular for a modular medical analysis system (20), comprising a sample chamber (2), wherein a fluorescent dye (4) is contained in the sample chamber (2) and wherein the fluorescent dye (4) is adhesive to a specific target molecule.

The invention relates to a filter device comprising a first filter carrier (10) with x first optical filters (11, 12) and a second filter carrier (20) with y second optical filters (21 - 23), wherein x and y each independently have a value of at least 2, and wherein the filter carriers (10, 20) are arranged to arrange one filter (11, 12, 21 - 23) of each filter carrier (10, 20) in a beam path of the filter device.Each first optical filter (11, 12) allows light of a respective first wavelength band (71, 72) to pass through, and each second optical filter (21-23) allows light of x respective second wavelength bands (81-86) to pass through, wherein the first wavelength bands (71, 72) do not overlap, the second wavelength bands (81-86) do not overlap, and each first wavelength band (71, 72) contains one second wavelength band (81-86) of each of the second optical filters (21-23). ​​An optical analysis device comprises a light source, a sample carrier, and an optical sensor. The filter device is arranged in a beam path between the light source and the sample carrier and / or in a beam path between the sample carrier and the optical sensor.In a method for operating the optical analysis device, a sample is arranged on the sample carrier, excited to fluorescence by means of the light source, and light emitted by the sample is detected by the optical sensor, whereby a fluorescence analysis of the sample is carried out in x · y optical channels.

The present invention relates to a CRISPR-Cas12a-based exosomal miRNA one-pot analysis method and device using an hourglass structure. The present invention provides an miRNA analysis method and device which can continuously perform the isolation and amplification of exosomes and the analysis of CRISPR Cas signals in a single reaction vessel by using multifunctional nanoparticles and an hourglass structure. The analysis method according to the present invention is characterized by: using an exosome isolation method using magnetic nanoparticles and an immune response without external equipment such as a centrifuge, thereby enabling selective isolation of only human cell-derived exosomes in a simple manner and at low cost; amplifying miRNA with CRISPR RNA (crRNA) instead of an aptamer, thus simplifying the amplification process; and analyzing a signal generated when a chromogenic enzyme immobilized via DNA is cleaved by Cas protein, thereby enabling analysis with the naked eye without separate fluorescence analysis equipment.

The invention relates to the technical field of pathogen optical detection, and discloses a multiple immunofluorescence analysis method for detecting multiple microbial pathogens. The method comprises the following steps: acquiring multi-modal fluorescence data of a sample surface through a bionic visual perception system, and generating a pathogen surface distribution heat map by using a deep neural network to identify a high-risk area. The system dynamically regulates and controls an optical detection array to emit a coding light signal into a sample according to a region division result, and reconstructs an internal three-dimensional structure of a pathogen by analyzing fluorescence response. Surface and internal data are integrated through a space-time alignment mechanism, fusion analysis is carried out by adopting a graph neural network, and a detection confidence score is generated to drive an adaptive decision. According to the method, accurate and dynamic detection of pathogens from the surface to the interior is realized, and the detection depth and the result reliability of a hidden target in a complex sample are improved.

1. Name of the product in this design: Graphical User Interface for Mechanical Debugging Demonstration of Electronic Equipment. 2. Intended use of this design: for use in electronic devices. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Purpose of the graphical user interface: Used for mechanical debugging in fluorescence analysis systems. Clicking the functional buttons on the interface in the main view enables interaction; for example, clicking any menu button on the left side of the interface will display the corresponding information.

The utility model discloses a reagent card carrying mechanism for a fluorescence analyzer, which is characterized in that first direction guide rail assemblies are arranged on two sides of an in-vitro first direction movement module, a first direction driving motor body is arranged at the end part of a first direction lead screw assembly, and the first direction movement module realizes horizontal transverse movement; a second direction guide rail assembly is arranged below the second direction lead screw assembly, a second direction driving motor body is arranged at the end of the second direction lead screw assembly, a third direction guide rail is arranged outside the third direction lead screw assembly, a third direction driving motor body is arranged at the end of the third direction lead screw assembly, and the third direction movement module achieves vertical lifting. According to the reagent card carrying mechanism for the fluorescence analyzer, through movement in three directions, the electric clamping hand main body can be moved to different positions, so that a reagent card is accurately positioned and clamped, the reagent card is stably clamped by using the clamping hand main body, and the clamping stability is improved.

The present invention provides a method for analyzing a halogenated structure in a composite semipermeable membrane. The method for analyzing a composite semipermeable membrane according to the present invention includes: a step A for immersing the composite semipermeable membrane in a solution containing alkali and at least one of nicotinamide and a derivative thereof; and a step B for performing fluorescence analysis on the solution obtained in step A.

The invention discloses a three-dimensional fluorescence analysis device for a river monitoring station. A first Y-shaped pipe is fixedly connected to a water inlet of a water quality three-dimensional fluorescence analysis equipment main body mounted in a river monitoring station, impurity removal boxes are fixedly connected and communicated with inlets and outlets in the two ends of the first Y-shaped pipe, and the same second Y-shaped pipe is fixedly connected and communicated with the ends, away from the inlets, of the two impurity removal boxes; the end, away from the two impurity removing boxes, of the second Y-shaped pipe is connected into an external river channel through a water pumping pipe, and the top openings of the two impurity removing boxes are movably sleeved with box covers. In the cleaning device, when a filter screen on a mounting framework in an impurity cleaning box needs to be lifted out and replaced, two stop blocks are manually operated to promote a rack to move leftwards on a guide seat, so that the rack can synchronously drive two first gears, and the first gears are promoted to respectively drive second gears; and the states of the two valve plates are quickly switched.

The invention provides a flow analysis system signal baseline estimation method and system, equipment and a medium, all calculations are carried out in an FPGA (Field Programmable Gate Array) in parallel and finished in an assembly line without software delay, and the method and the system can be directly used for triggering subsequent real-time signal processing or cell sorting decisions; through the triple mechanisms of hardware pre-filtering, extreme value elimination and recursive smoothing, various noises are effectively inhibited, and the baseline estimation variance is significantly reduced; an adder, a comparator and a register are mainly used in the algorithm, a complex multiplication divider or a large number of storage units are not needed, FPGA resource occupation is small, and power consumption is low. The provided stable and accurate double-baseline value can be used as the estimation input of a noise covariance matrix in a weighted least square (WLS) spectrum unmixing algorithm, and the unmixing precision and the signal-to-noise ratio of multicolor fluorescence analysis are directly improved; due to pure digital hardware implementation, the method can be easily transplanted to different types of flow cytometers or other optical particle analysis equipment.

This invention relates to the application and method of a fluorescent ionic liquid in the detection of nitrophenol isomers. The fluorescent ionic liquid is (poly)1,3-di(N-methylimidazolyl)propane-1,4-naphthalenedicarboxylate, and the structure of the compound is shown in the following formula. The preparation method is as follows: First, 30 mL of NaOH solution (3 mol / L) and 9.47 g of 1,4-naphthalenedicarboxylate are added sequentially to a single-necked flask, and the mixture is refluxed at 30 °C for 24 h to obtain a clear brown solution, which is a sodium 1,4-naphthalenedicarboxylate solution. Next, 8 mL of 1,3-dibromopropane, 12 mL of N-methylimidazolium, and 50 mL of acetone are added sequentially to a three-necked flask. The mixture is stirred and refluxed at 45 °C for 20 h, washed three times with 50 mL of acetone each time, and filtered to obtain a white solid powder. Finally, the prepared sodium 1,4-naphthalenedicarboxylate solution and 8.3 g of the white solid product obtained in step two are added sequentially to a single-necked flask, and the mixture is stirred and reacted at 50 °C for 24 h. The prepared solution was rotary evaporated, dried, diluted with anhydrous ethanol, filtered, and dried again to obtain the target product (poly)1,3-di(N-methylimidazolyl)propane 1,4-naphthalenedicarboxylate. This invention is simple to operate and has high fluorescence intensity and sensitivity, making it suitable for fluorescence analysis and identification of nitrophenol isomers.

Provided is a cleaning sheet excellent in cleanliness, foreign particle-removing performance, and transfer performance. The cleaning sheet includes a cleaning layer. The cleaning layer contains a polyimide-based resin. The cleaning layer has an amount of a metal transferred to a silicon wafer of 1×1011 atoms / cm2 or less, which is measured by a total reflection X-ray fluorescence analysis method.

This invention belongs to the field of fluorescence analysis technology and relates to the application of a sulfur quantum dot fluorescent probe in tryptophan detection. This invention utilizes sulfur quantum dot probes to explore exogenous tryptophan cell imaging technology, studies the role of ultraviolet light in cell imaging, and enhances the fluorescence signal intensity of the sulfur quantum dot fluorescent probe by ultraviolet light irradiation, effectively achieving in vitro quantitative detection of tryptophan and highly sensitive, highly selective in situ detection in living cells. This invention can be used for the detection of tryptophan in vitro and in living cells, and has promising clinical application prospects in the biomedical field.

The invention discloses a fluorescence analysis and detection method and system based on quantum dots, and belongs to the technical field of analysis and detection. According to the method, functional modified quantum dots are used as fluorescent probes, a fluorescence detection system is constructed through specific recognition reaction, and high-sensitivity and high-specificity detection of a target detection object is realized by combining collection and quantitative analysis of fluorescence signals. The corresponding system comprises an excitation light source module, a sample reaction module, a fluorescence detection module, a data processing module and a control module, all the modules work cooperatively, key parameters in the detection process can be precisely regulated and controlled, and the detection precision and stability are improved. The method solves the problems of poor probe stability, low quantum yield and high detection limit in a traditional fluorescence detection method, has the advantages of high detection speed, high specificity and wide application range, and can be widely applied to the fields of food safety detection, environmental pollutant monitoring, biomedical diagnosis and the like.

A method for calibrating fluorospectrometric measurements obtained on a spectrometric device is disclosed. Calibration methods of this disclosure utilizes non-fluorescent emissions of water, primarily Raman scattering signals of water, as a calibration reference. For aqueous samples containing minute amounts of fluorescent analytes, the spectrometric measurements of samples obtained will contain both fluorescent emission and non-fluorescent emission, including water Raman scattering. Calibration factors based on the water Raman peaks are provided. Spectra of samples may be compared to each other by calibrating the spectral readings against the calibration factors. Also disclosed are apparatus useful for performing the calibration methods, including lens array for enhancing the optics of spectrometric measurements and water Raman calibration methods, spectrometric devices configured to perform the calibration methods, and computer-readable medium encoding the calibration method.

The utility model discloses an X-ray fluorescence analyzer which comprises a shell, an X-ray tube, a detector, an isolating membrane and an isolating piece, a measuring cavity is defined by the shell, the X-ray tube and the detector extend into the measuring cavity, a first through hole is formed in the bottom end face of the shell so that X-rays can be emitted out and irradiate a sample, and the isolating membrane covers the first through hole so as to isolate the measuring cavity from the sample; an air gap is formed between the bottom end of the shell and the sample, the separator is located between the shell and the sample, and the upper end face of the separator is connected with the bottom end face of the shell; a second through hole is formed in the isolation piece, and the second through hole and the first through hole are correspondingly arranged so that the X-ray can penetrate through the isolation piece and the second through hole to irradiate the sample; an air flow channel is formed in the isolation piece so that air can be guided into the second through hole to be blown. According to the X fluorescence analyzer, the problem that the scanning measurement assembly is polluted is avoided; the air gap between the assembly and the sample is purged by the purge gas, unstable components in the air gap are taken away, and the signal-to-noise ratio of detection is improved.

The present application relates to a fluorescence analysis device and a fluorescence analysis method. The fluorescence analysis device is a device that analyzes measurement data before operation processing, which is obtained by detecting fluorescence generated when excitation light is respectively irradiated on a plurality of samples each labeled with any one of a plurality of fluorescent pigments, through a plurality of detection channels, and identifies the samples based on the kind of the fluorescent pigment labeled. The fluorescence analysis device includes a first determination section that determines whether the plurality of samples can be separated into two or more groups based on measurement data obtained by one or more of the plurality of detection channels, and a first separation section that separates any one group from among the plurality of samples when the first determination section determines that the plurality of samples can be separated into two or more groups.

The invention provides a glass raw material content detection method for improving fluorescence analysis accuracy, which comprises the following steps: S1, mixing a glass raw material sample with a flux to form a mixture; s2, putting the mixture into a crucible, adding a release agent, then carrying out melting treatment, and cooling to obtain a test sample; and S3, analyzing the test sample by using an X-ray fluorescence spectrophotometer, and correcting and making a standard working curve through a correction formula. In the fluorescence analysis process of the melting slide preparation method, the influence of the residual quantity of the release agent on the analysis result is avoided by monitoring the interference of the residual quantity of the release agent on the to-be-detected element and correcting the analysis result; and the detection accuracy and the anti-interference capability are improved.

A fluorescence detection system, including apparatus and methods, suitable for qPCR and other fluorescence-based analyses. The system may comprise various components, including a stage, an illumination module, a detection module, and an optical relay structure. The stage may be configured to support a sample holder. The illumination module may include one or more discrete light sources configured to produce excitation light. The detection module may be configured to detect fluorescence emission light produced, in response to the excitation light, by a fluorescent sample positioned in the sample holder. The optical relay structure may include a beamsplitter assembly configured to direct the excitation light from the illumination module along an illumination path to the sample holder and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder.

A fluorescence analysis device for discriminating samples in accordance with a type of fluorescent dye labeled by analyzing measurement data before an arithmetic process obtained by detecting fluorescence generated when a plurality of samples labeled with any of a plurality of fluorescent dyes are each irradiated with excitation light by a plurality of detection channels comprises a first determination unit configured to determine whether or not the plurality of samples can be separated into two or more populations based on the measurement data by any one or more of the plurality of detection channels; and a first separation unit configured to separate one population from the two or more populations when the first determination unit determines that the plurality of samples can be separated into the two or more populations.

The invention relates to a filter device comprising: a first filter carrier (10) having x first optical filters (11, 12); and a second filter carrier (20) having y second optical filters (21-23), wherein x and y each independently have a value of at least 2, and wherein the filter carriers (10, 20) are each designed such that one filter (11, 12, 21-23) of each filter carrier (10, 20) can be positioned in an optical path of the filter device. Each first optical filter (11, 12) transmits light in a first wavelength band (71, 72), and each second optical filter (21-23) transmits light in x second wavelength bands (81-86), wherein the first wavelength bands (71, 72) do not overlap, the second wavelength bands (81-86) do not overlap, and each first wavelength band (71, 72) contains a second wavelength band (81-86) from each of the second optical filters (21-23). An optical analysis device comprises a light source (41), a sample carrier (42), and an optical sensor (43). The filter device is arranged in an optical path between the light source (41) and the sample carrier (42) and / or in an optical path between the sample carrier (42) and the optical sensor (43). In a method for operating the optical analysis device, a sample (50) is arranged on the sample carrier (42), is excited to fluoresce by means of the light source (41), and light emitted by the sample (50) is detected by the optical sensor (43), wherein a fluorescence analysis of the sample is carried out in x · y optical channels.

The invention provides a fluorescence-colorimetric dual-mode lateral chromatography test strip platform and application, and belongs to the technical field of test strip detection. The fluorescence-colorimetric dual-mode lateral chromatography test strip platform comprises a fluorescent microsphere-gold nanoparticle conjugate and a DNA (Deoxyribose Nucleic Acid) signal amplification tree solution. The platform combines dual-mode detection of gold nanoparticles and fluorescent microspheres, can be observed by naked eyes, also can be used for quantitative analysis by a fluorescence analyzer, and has the characteristics of portability, low cost and high sensitivity. Meanwhile, the platform is a piece of lateral flow test paper based on a dendritic DNA signal amplification system, and can be used for rapidly and sensitively detecting pathogenic bacteria genes of the sugarcane pokkah boeng disease.

To provide an analysis of vibration fluorescence from a biological cell.SOLUTION: A method and a system are for analyzing vibration fluorescence showing vibration ion flux associated with one or more biological cells. An exemplary method (50) may include a step (54) of detecting fluorescence (91) from one or more biological cells (72) to generate a series of data points (120) describing a vibration pattern (110). A series of gradients may be calculated (60) with respect to the vibration pattern (110). For example, sliding window (122) may be used to define a subset (126) of a series of data points (120) from which the series of gradients are calculated. The peaks (118, 150a, 150b) of the vibration pattern (110) may be identified using a series of gradients (62).SELECTED DRAWING: Figure 2

The utility model relates to a material moving device which is characterized by comprising a support, a horizontal moving mechanism, a lifting mechanism and a clamping mechanism. Wherein the horizontal moving mechanism is arranged on the bracket; the lifting mechanism is connected to the driving end of the horizontal moving mechanism, and the horizontal moving mechanism is used for driving the lifting mechanism to move in the horizontal direction; the clamping mechanism is connected to the driving end of the lifting mechanism, and the lifting mechanism is used for driving the clamping mechanism to move in the vertical direction; and the clamping mechanism is used for clamping the X fluorescence analysis sample box filled with the sample. The material moving device provided by the utility model is simple in structure, low in manufacturing cost and high in reliability, can clamp a sample through the clamping mechanism to move in the horizontal and vertical directions, replaces manual transfer of the sample, and improves the automation level during sample detection.

The application discloses a method for GI and GII type norovirus on-site typing detection, comprising the following steps: in the presence of primers, carrying out double RT-RPA reaction on a template, then adding the amplification product after reaction into a CRISPR / Cas12a detection system of GI and GII type norovirus respectively, carrying out fluorescence analysis, and completing GI and GII type norovirus on-site typing detection. The application covers each subtype of norovirus GI and GII, has no cross reaction with common gastroenteritis viruses such as rotavirus and enterovirus, can effectively distinguish the two genotypes of GI and GII, and has no off-target phenomenon, and the minimum detection limit of the application for the GI and GII genotype norovirus is 10 2 copies / µL and 1 copies / µL respectively. The whole detection process can be completed within 35 min under the condition of 37 DEG C constant temperature, can be directly used for on-site detection by means of an ultraviolet flashlight, and does not need laboratory instruments and professional operation.