Automatic multichannel flow-like image fluorescence analysis system

Abstract

The invention discloses an automatic multichannel flow-like image fluorescence analysis system including an integrated fluorescence excitation light source device, a sample stage device, a microscopic imaging device, an automatic control system and an analysis and processing system, wherein the automatic control system is connected with the integrated fluorescence excitation light source device, the sample stage device and the microscopic imaging device, and is used for respectively controlling the integrated fluorescence excitation light source device, the sample stage device and the microscopic imaging device; a microscopic optical system is formed by the integrated fluorescence excitation light source device, the sample stage device and the microscopic imaging device; the analysis and processing system is connected with the microscopic imaging device and is used for analysis and processing of images collected by the microscopic imaging device. The automatic multichannel flow-like image fluorescence analysis system can rapidly collect and analyze imaging information of a large number of samples based on the microscopic imaging technology, and also adopts a density function algorithm for analysis, so as to simultaneously obtain morphological iconography analysis results of all cells and density function flow-like data analysis results based on the large sample size.

Description

[0001] This application claims the priority of the Chinese patent application with the application number 201510376530.0 and the invention title "Integrated Fluorescent Excitation Light Source Device" submitted to the China Patent Office on July 1, 2015, the entire contents of which are incorporated in this application by reference. technical field [0002] The invention relates to the technical field of fluorescence analysis, and more specifically relates to an automatic multi-channel flow-like image fluorescence analysis system. Background technique [0003] Cell analysis is of great significance in both basic biological research and clinical diagnostic applications. Cell analysis and research are subdivided into many directions and applications. From the perspective of quantity and nature, it can be divided into qualitative research and quantitative research. [0004] In the prior art, the qualitative research on cells starts from the traditional observation and measuremen...

AI Technical Summary

Problems solved by technology

The advantage of the flow fluorescence analysis method is that it can quickly realize the collection of a large number of cell samples and various fluorescent characteristic signals. Based on the principle of density function probability classification analysis, it can finally achieve qualitative research on samples. The imaging of a single cell under the environment cannot directly conduct in-depth observation and judgment on a single cell, and its judgment on the nature of the cell is a probabilistic judgment rather than an absolute judgment.

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