Fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide

A nano-copper oxide and peroxidase technology, applied in analytical chemistry and nano fields, can solve the problems of poor selectivity, interference, false increase of blood glucose measurement value, etc., and achieve low cost, low sample demand and good reproducibility. Effect

Inactive Publication Date: 2014-04-16
FUJIAN MEDICAL UNIV
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Problems solved by technology

Commercial blood glucose meters are mostly completed in one step, but the selectivity is poor, and they are seriously interfered by substances such as maltose, xylose, and galactose, which often lead to a false increase in the blood glucos

Method used

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  • Fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide
  • Fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide
  • Fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide

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Effect test

example 1

[0038] The specific preparation steps of nano-copper oxide are as follows: (1) Take 150 ml of 0.02 mol / L copper acetate solution and 0.5 ml of glacial acetic acid into a three-necked flask equipped with a condenser, stir and heat to boiling; (2) Quickly add 0.04 g / ml sodium hydroxide solution 10 ml, continue to stir for 5 minutes after adding, and obtain brown copper oxide precipitate; (3) Centrifuge the black copper oxide precipitate obtained from the reaction, wash with absolute ethanol three times, and dry under reduced pressure, that is A nano-copper oxide powder with a diameter of 6 nm was obtained.

example 2

[0040]0.8 mL of terephthalic acid with a concentration of 18.75 mmol / L, 0.5 mL of hydrogen peroxide with a concentration of 1 mol / L and 50 μL of nano-copper oxide prepared in Example 1 with a concentration of 40 mg / L were added to 3.65 mL of In 200 mmol / L phosphate buffer (pH 3-10) of different pH, mix and shake well and place in a warm bath at 45 °C. After 20 minutes, measure its fluorescence intensity at 421 nm (excitation wavelength is 315 nm). Such as figure 1 As shown, the fluorescence intensity reached a maximum at pH 7.0.

example 3

[0042] 0.8 mL of terephthalic acid with a concentration of 18.75 mmol / L, 0.5 mL of hydrogen peroxide with a concentration of 1 mol / L and 50 μL of nano-copper oxide prepared in Example 1 with a concentration of 40 mg / L were added to 3.65 mL of In 200 mmol / L phosphate buffer (pH 7.0), mix and shake well, place in different temperature (20-55 ℃) warm bath, measure its fluorescence intensity at 421 nm after 20 minutes (excitation wavelength is 315 nm) . Such as figure 2 As shown, the fluorescence intensity reached the maximum at 45 °C.

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Abstract

The invention discloses a fluorescence analysis method (/b) for determining glucose by employing (b) nanometer copper oxide as simulated peroxide. The fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide is characterized by comprising the following steps: firstly, mixing glucose, glucose oxidase and phosphate buffer in a warm bath, and then adding the phosphate buffer; continuing to carry out warm bath on terephthalic acid and the nanometer copper oxide, wherein the fluorescence excitation wavelength and the emission wavelength of a reaction product of the mixing solution are 315nm and 421nm, the linear range of glucose content measurement is 3-120mumol/L, and the detection limit is 1.94mumol/L. Therefore, the method can be applied to determination of blood sugar concentration.

Description

technical field [0001] The invention relates to a fluorescence analysis method for measuring glucose by simulating peroxidase using nanometer copper oxide, which belongs to the field of analytical chemistry and nanotechnology. Background technique [0002] At present, there are o-toluidine method and enzymatic method for clinical determination of blood glucose. The o-toluidine method is simple in operation and low in cost, but has poor specificity, and the o-toluidine reagent is unstable and highly toxic. The enzymatic method is an analysis method that utilizes the highly selective catalysis of enzymes. According to different enzyme types, enzymatic methods can be divided into hexokinase method, glucose dehydrogenase method and glucose oxidase-peroxidase method. Commercial blood glucose meters are mostly completed in one step, but the selectivity is poor, and they are seriously interfered by substances such as maltose, xylose, and galactose, which often lead to a false inc...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈伟胡爱玲何少斌邓豪华李光文
Owner FUJIAN MEDICAL UNIV
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