137 results about "Hexokinase" patented technology

The invention discloses a creatine jubase MB isozyme activity detection reagent which comprises sulfide-oxidizing coenzyme, 6-phosphaogluconate dehydrogenase, sulfhydryl reagent, magnesium salt, glucokinase or hexoxinase, anti-CK-M antibody, phosphocreatine, adenosine diphosphate, glucose and glucose-6-phosphate dehydrogenase. The preparation method of the reagent is as follows: dissolving all components in a buffer solution with pH of 5.0 to 9.5. The creatine jubase MB isozyme activity detection reagent can enlarge detection sensitivity several times; and the determination result has high precision and degree of accuracy.

The present disclosure relates to host cells containing two or more of a recombinant cellodextrin transporter, a recombinant cellodextrin phosphorylase, a recombinant β-glucosidase, a recombinant phosphoglucomutase, or a recombinant hexokinase; and to methods of using such cells for degrading cellodextrin, for producing hydrocarbons or hydrocarbon derivatives from cellodextrin, and for reducing ATP consumption during glucose utilization.

The invention relates to a serum AFU detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises 50-500 mmol/L of a buffer solution (pH 4.0-4.5), 1-50 g/L of a surfactant, 1-20 g/L of an anti-interference agent and 0.1-100 g/L of a preservative; the reagent 2 comprises 50-500 mmol/L of a buffer solution (pH 7.0-7.5), 2-60 g/L of glucose, 1-20 KU/L of hexokinase, 1-20 KU/L of glucose-6-phosphate dehydrogenase, 0.3-5 g/L of magnesium sulfate, 0.2-5 g/L of a substrate, 1-100 mmol/L of a stabilizer, 0.1-40 g/L of a protective agent and 0.1-100 g/L of a preservative. With the adoption of the kit, problems of the poor substrate stability, the poor anti-interference capacity and the lower sensitivity which exist universally are solved.

The invention relates to an Aspergillus niger gene engineering strain efficiently producing malic acid. The Aspergillus niger gene engineering strain is an Aspergillus niger gene engineering strain which knocks out a citric acid transporter gene cexA, and overexpresses a glucose transporter gene mstC, a hexokinase gene hxkA, a phosphofructokinase gene pfkA, and a pyruvate kinase gene pkiA which are derived from Aspergillus niger. The efficiency for producing malic acid of the Aspergillus niger gene engineering strain is significantly improved, the output on the eighth day through fed-batch fermentation in a fermentation tank reaches 195.72-210 g/L, a conversion rate of malic acid to glucose reaches 1.59-1.64 mol/mol, and a fermentation cycle is shorter by one day compared with a starting strain, namely the fermentation cycle is shortened from original 9 days to 8 days. An excellent strain for the preparation of malic acid by microbial fermentation methods is provided.

The invention discloses a quantitative fructose assay kit which comprises inorganic acid deproteinized extract A, inorganic base deproteinized extract B, fructose calibration solution, a reagent 1 containing 0.001-0.1mol/L adenosine triphosphate sodium salt, a reagent 2 containing 1-100KU/L hexokinase and 1-100KU/L glucose-6-phosphate dehydrogenase, and a reagent 3 containing 0.001-0.1mol/L nicotinamide adenine dinucleotide. The seminal plasma fructose assay method comprises the following steps: respectively adding the reagent 1 and the reagent 2 to deproteinized seminal plasma and the fructose calibration solution, and mixing uniformly; reacting at the temperature of 10-40 DEG C for 5-120 minutes, then reading the absorbance respectively at the wavelength of 280-400nm; adding the reagent 3 respectively, and mixing uniformly; reacting under the same conditions and reading the absorbance; and calculating the difference between the absorbance read at the first time and the absorbance read at the second time, and comparing or calculating the absorbance of a seminal plasma specimen and the fructose calibration solution to obtain the concentration of the seminal plasma fructose. The kit and the method can be used for quantitative determination of fructose in sera, plasma, body fluid, food and solid extracting solution, the methodology is special, unique, clean and environment-friendly, manual operation and automatic batch assay can be realized, and the kit and the method are easy to popularize and apply clinically.

System and method for radiographic imaging of tissue using a non-radioactive, radio-opaque imaging agent that accumulates intracellularly in tissue in proportion to its functional, or physiological, activity. In one embodiment, the imaging agent is a cell-membrane permeable, radio-opaque, high affinity ligand for the intracellular enzyme hexokinase. The imaging agent is administered to a patient, and after an accumulation interval, radiographic images are acquired. The imaging agent preferentially accumulates in malignant tissue and increases its radio-opacity because of its elevated glucose metabolic rate relative to benign and normal tissue. The tissue being examined is transilluminated by X-ray beams with preselected different mean energy spectra, and a separate radiographic image is acquired during transillumination by each beam. An image processing system performs a weighted combination of the acquired images to produce a single displayed image. The image processing procedure isolates the radiographic density contributed solely by differential accumulation of the imaging agent in malignant, benign, and normal tissue. The system and method thus provides a functional image displayed with the anatomical detail and spatial resolution of a radiographic image. The viewer may interactively control the relative proportion of radiographic density contributed by imaging agent, soft tissue, and bone to the displayed image, allowing the display of functional and anatomical information in complete registration, and facilitating localization of malignant tissue in relation to nearby anatomical structures. In other embodiments, the system and method may be used to detect enzymes, nucleic acids, coenzymes, fatty acids, and other cellular targets in diagnostic imaging applications.

The invention relates to a method for synthesizing 6-hexose phosphate by employing an enzymic method. The method comprises the following steps: with hexose as a substrate, polyphosphates and/or triphosadenine salt as phosphate donors, putting a reactant into a pH buffer system; adding inorganic polyphosphoric acid hexokinase and cofactors to the buffer system; and synthesizing the 6-hexose phosphate under the catalysis action of the inorganic polyphosphoric acid hexokinase. Compared with a chemical method, the method for synthesizing the 6-hexose phosphate by employing the enzymic method is green and safe; compared with other methods for synthesizing the 6-hexose phosphate by other enzymic methods, the polyphosphates and/or triphosadenine salt can be used as phosphate donors by the inorganic polyphosphoric acid hexokinase disclosed by the invention. The triphosadenine salt with high cost is replaced with polyphosphates, so that the problem of high production cost is solved.

[PROBLEMS] To provide lipase activity determination reagents which function by an enzymatic method. The reagents are easy to use in an ordinary clinical examination, have excellent handleability, and are excellent in accuracy and reproducibility. [MEANS FOR SOLVING PROBLEMS] Lipase activity is determined with any of reagents which comprise a low-concentration buffer and a diglyceride dissolved therein. The diglyceride is used as a substrate for lipase, whereby the liquid reagents can have long-term storage stability. One of the reagents converts a monoglyceride yielded by a lipase reaction into glycerol with the aid of monoglyceride lipase, and further contains glycerol kinase, pyruvate kinase, lactate dehydrogenase, reduced NAD, ATP, and phosphoenol pyruvate. Another reagent converts a monoglyceride yielded by a lipase reaction into glycerol with the aid of monoglyceride lipase, and further contains glycerol kinase, glucose, ADP-dependent hexokinase, glucose-6-phosphate dehydrogenase, oxidized NAD or oxidized NADP, and ATP. A further reagent converts a monoglyceride yielded by a lipase reaction into glycerol with the aid of monoglyceride lipase, and further contains glycerol kinase, glycerol-3-phosphate oxidase, peroxidase, and a dye which colors in the presence of hydrogen peroxide.

The invention relates to the technical field of detection for GLU in the blood by adopting hexokinase, and in particular relates to a detection reagent for GLU in the blood, which is strong in stability and low in cost and adopts liquid hexokinase. The detection reagent adopts liquid double reagents, wherein the reagent I mainly contains a buffer solution, Mg<2+>, an ion balancing agent, a preservative, NAD (oxidized coenzyme I), a protective agent and a surfactant, and the reagent II contains a buffer solution, a protective agent, a surfactant, preservative, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH), ATP.NA2, and the like. In order to guarantee the stability of enzymes in the reagent, various protective agents including polyethylene glycol-20000, tween-80, FAD and the like are added in the reagent in a pertinence manner, in order to reduce the cost, especially the use of enzymes, various surfactants are added in the reagent, so that the emulsifying for enzymes is enhanced, the service efficiency for enzymes is improved, thus the cost of the reagent is greatly reduced, and the detection reagent is particularly suitable for being used and popularized in clinic.

The invention discloses an enzymatically grafted phosphorus-containing chitosan oligosaccharide based inflaming retarding finishing method of a protein fiber product, and belongs to the technical field of textile biology. The inflaming retarding finishing method aims at solving defects that the fiber damage and the like are easily caused when a conventional chemical method is used for carrying out the inflaming retarding finishing on the protein fiber product. According to the inflaming retarding method, the pretreatment of the protein fiber product is first carried out; chitosan oligosaccharide is catalyzed to be grafted on the surface of a protein fiber through tyrosinase; the chitosan oligosaccharide is catalyzed to be phosphorylated by combining with hexokinase; the inflaming retarding effect is given to the protein fiber product. The inflaming retarding finishing method comprises the following specific steps of (1), carrying out the pretreatment on the protein fiber product; (2), catalyzing the protein fiber to graft the chitosan oligosaccharide by the tyrosinase; (3), catalyzing the chitosan oligosaccharide to be phosphorylated by the hexokinase; (4), carrying out water scrubbing and oven-drying post treatment. The protein fiber product treated by the inflaming retarding method is ameliorated in inflaming retarding performance and improved in mechanical performance; in comparison with the inflaming retarding finishing of the conventional chemical method, the inflaming retarding finishing, by which the enzymatically grafted phosphorus-containing chitosan oligosaccharide is adopted, of the protein fiber product is low in energy consumption.

The invention discloses a synthesis method of oligosaccharides of a Lewis x unimer, a dimer and saliva acidifying derivatives thereof. The method comprises the steps that core framework compounds (V)to (IX) are built by enzymic method modular assembly 1 and enzymic method modular assembly 2; a Lex unimer and a dimer (I) to (II) are assembled by enzymic method modular assembly 3; finally, an sLexunimer and A dimer (III) to (IV) are built by enzymic method modular assembly 4. High region selectivity and high efficiency of the enzymic method synthesis are combined; the target molecule synthesisis realized at higher yield. Glycosyltransferase, riboside generation enzyme and hexokinase are from prokaryotic organisms; the advantages of high protein expression, wide substrate adaptability, high catalysis efficiency and the like are realized; the synthesis method can be used for large-scale preparation.

The invention discloses genetic engineering bacteria producing beta- carotene and a construction method of the genetic engineering bacteria. The construction method comprises the following steps of firstly knocking out alpha-glycerophosphate dehydrogenase gut2 genes in a glycolysis way in a beta-carotene synthetizing pathway in yarrowia lipolytica, then performing free expression on beta-hydroxyl-beta-methylglutaric acid erg13 genes having two endogenous copy numbers of the yarrowia lipolytica, and then performing integrant expression on the erg13 genes having two endogenous copy numbers of the yarrowia lipolytica at the position where the gut2 genes are knocked out; and performing free expression on endogenous hexokinase Hxk genes of the yarrowia lipolytica, and screening positive transformants to obtain the engineering bacterial strains producing beta-carotene yarrowia lipolytica. The bacterial strains are subjected to fermentation, culturing, extraction and separation, the beta-carotene is purified, and the content of the beta-carotene can reach 26.6mg/g cell dry weight.

The invention belongs to production of enzymic preparations and particularly relates to a method for preparing diagnostic hexokinase. The method includes the process steps of purifying and rejuvenating a strain, preparing fermentation liquor containing hexokinase by fermentation and purifying the fermented crude hexokinase enzyme. The method for preparing the diagnostic hexokinase has the advantages that the problems that the diagnostic hexokinase with independent intellectual property is not available domestically, imported enzymic preparations are high in price and inconvenient to use and the like currently are solved, the method is reliable in strain source, feasible, suitable for industrialized production and capable of filling the blank that the diagnostic hexokinase with the independent intellectual property is not available domestically, and major performance indexes of the diagnostic hexokinase prepared by the method are superior to those of the imported products.

The invention discloses a reagent kit for detecting glucose by a hexokinase method, which belongs to a reagent kit in a method for detection glucose containing enzyme. The reagent kit comprises four bottles of reagent I, one bottle of reagent II, and one glucose standard liquid, wherein the reagent I comprises oxalate with the concentration of 8.0mmol/L. By inhibiting the activities of endogenous lactate dehydrogenase and alpha-hydroxybutyrate dehydrogenase through the oxalate, the reagent kit controls an interference reaction, increases the specificity of the reaction, and improves the accuracy of detection results. The reagent kit is slightly affected by the volume ratio of sample reagents and the reaction time during the detection, and the linear range can reach 37.4mmol/L. A use method and the range of the reagent kit are the same as those of the prior hexokinase method; and the reagent kit can not increase the burden of experimental personnel, almost does not increase the cost of reagents, is economical, convenient and easy, and is the reagent kit which detects the glucose by the hexokinase method with higher accuracy.

The invention discloses a carbon dioxide kit employing enzymatic cycling assay. The kit is composed of a NADH analogue cyclic regeneration system and a carbon dioxide test reagent, wherein the NADH analogue cyclic regeneration system consists of 6-phosphogluconate dehydrogenase, hexokinase, glucose and adenosine triphosphate disodium. The carbon dioxide kit is simple to prepare and low in cost, and the NADH analogue cyclic regeneration system in the kit can prolong the storage time of the kit and effectively improves the stability, accuracy and precision of testing.

The invention relates to a separation and purification method of a Basidiomycetes antifungal component; the antifungal component is composed of two amide-type substances, and the molecular weight of the two substances is 5732.39 and 7187.01; the separation and purification process comprises the following steps: Basidiomycetes entity authentication, strain separation and purification, strain identification, mycelium liquid culture, antifungal component extraction, antifungal component separation, antifungal component purification. The method can inhibit chrysosperma mycelium growth and spore germination with 100 percent of inhibition rate, and has obvious inhibiting function to Pinus sylvestris germ at the same time. The Basidiomycetes antifungal substance affects the activity of seven enzymes of pathogenicbacteria, reduces the activity of hexokinase, improves the activity of pyruvate kinase, succinic acid, lactate dehydrogenase and ATP enzyme, and speeds up the respiration of germs.

The invention relates to a natrium diagnosis / determination reagent kit which utilizing a enzyme colorimetry method and an enzyme-linked method technology, simultaneously the invention also relates to a method principle for determining the natrium concentration, the composition and the components of a reagent, belonging to the technical field of the medicine / food / environment examination and determination. The main components of the reagent kit of the invention comprise buffer solution, coenzyme, deoxycholate citrate lactose sacharose agar, adenosine triphosphate, magnesium chloride, Beta-galactosidase, hexokinase, dextrose-6-phosphate dehydrogenase and stabilizer. A sample is mixed with the reagent according to a certain volume to lead to perform the enzymatic reaction, a reacting substance is put under an ultraviolet / visible light analyzer to examine the rising speed of absorbance at the position of a 340nm dominant wavelength, thereby detecting and calculating the natrium concentration value. Through the adoption of the invention, the required determined results can be obtained completely through the ultraviolet / visible light analyzer.

The invention mainly aims at providing a creatine kinase isozyme bi-reagent and a preparation method thereof. The reagent I is prepared from the following components: 90 to 120mmol/L of imidazole buffer solution, 15 to 25mmol/L of N-acetylcysteine, 25 to 35mmol/L of phosphocreatine, 1 to 10mmol/L of adenosine diphosphate, 1 to 10mmol/L of triphosadenine, greater than 1.5KU/L of glucose-6-phosphatedehydrogenase, greater than 2.5KU/L of heterophosphatase and greater than 2.0KU/L of creatine kinase isozyme antibody; 0.01 to 0.05 percent by mass concentration of polyvinylpyrrolidone, 0.01 to 0.05percent by mass concentration of tris(nonylphenyl) phosphate, 0.01 to 0.02 percent by mass concentration of lauryl dihydroxyethyl amine oxide, 0.05 percent of isomeric sodium ascorbate and the balance of water; the reagent II is prepared from the following components: by adopting a reagent II solution as a basis, 1 to 5mmol/L of nicotinamide adenine dinucleotide phosphate, 10 to 30mmol/L of glucose and the balance of water. In the case of storing for 14 days at 37 DEG C, the reagent stability is still relatively high, and measurement value deviation of the reagent is less than 10 percent.

The invention discloses a method for improving germination and growth of corn seeds and relates to the field of crop planting. The method aims to solve the technical problems of low germination rate and long germination period of existing corn seeds. The method comprises the steps of 1, sterilizing the surfaces of the seeds; 2, preparing a seed soaking agent; 3, performing vibrated seed soaking treatment. The method can shorten the average time of seed germination and increase the germination rate, germination vigor, germination index and vigor index of the seeds. The growth and elongation ofa root system and germs are boosted; the activity of hexokinase and uridine diphosphate glucose pyrophosphorylase is obviously increased, the activity of aldolase is reduced, the activity of phosphofructokinase is maintained at a relatively normal level, the glycolysis metabolism and the biosynthesis process of cell walls are promoted, and metabolic products and energy are provided for the germination and growth of the seeds. The method is used for promoting the germination and growth of the corn seeds.

The invention provides a compound or a pharmaceutically acceptable salt thereof or a stereoisomer or isotope labeled compound thereof. The compound has a good inhibition effect on hexokinase. Meanwhile, the invention also provides a pharmaceutical composition containing the compound or the pharmaceutically acceptable salt thereof or the stereoisomer or isotope labeled compound thereof. The invention further discloses an application of the compound or the pharmaceutically acceptable salt or the stereoisomer or isotope labeled compound in preparation of drugs for treating diseases such as T1D, T2D, LADA, EOD, YOAD, MODY, dystrophy related diabetes and gestational diabetes.