Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for detecting reagents and glucose, which is applied in the field of concentrated glucose detection reagent freeze-dried microspheres and its preparation, to achieve the effects of simplified operation steps, uniform size and shape, and easy packaging
Active Publication Date: 2014-12-10
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF7 Cites 14 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] The object of the present invention is to provide a kind of concentrated glucose detection reagent freeze-dried microsphere and preparation method thereof, to solve the problems existing in prior art liquid and freeze-dried powder glucose detection reagent
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0042] A method for preparing concentrated glucose detection reagent freeze-dried microspheres, comprising the following steps:
[0043] (1), prepare glucose detection reagent;
[0044] (2), add lyoprotectant and plasticizer, filter to remove insoluble matter after fully dissolving, vacuum degassing removes air bubble;
[0045] (3) Micro-droplets are prepared by using a liquid separation system that can be accurately quantified, and the droplets are dropped into liquid nitrogen, and frozen reagent microspheres are formed after being cooled by liquid nitrogen;
[0046] (4) Transfer the frozen reagent microspheres into a lyophilizer to freeze-dry to prepare the detection reagent lyophilized microspheres.
[0047] The volume of the droplet in step (3) is precisely controlled by a liquid separation system, the size of the droplet is 2-10ul, and the size error is within 2%.
[0048] The glucose detection reagent freeze-dried microspheres that can be used for instant detection ins...
[0067] The surfactant TrionX-100 in the glucose detection reagent can effectively control the generation of air bubbles during the dissolution of the reagent microspheres; the lyoprotectant in the reagent can be sucrose, trehalose, polyethylene glycol, bovine serum albumin, poly One or more of vinylpyrrolidone, mannitol, and dextran, the content of which can be determined according to the morphology and solubility of the freeze-dried microspheres of the detection reagent.
[0068] (2) Use th...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a freeze-drying concentrated glucose detection reagent microsphere and a preparation method thereof. The freeze-drying microsphere comprises a triethanolamine buffer solution, ATP, NADP, hexokinase, 6-glucose dehydrogenase phosphate, a preservative, trehalose, polyethylene glycol 8000, polyethylene glycol 20000, TrionX-100 and the like. The preparation method comprises the following steps: (1) preparing a solution, and performing filtration and degassing; (2) preparing droplets by using a precise quantification liquid separation system, dropwise adding the droplets into liquid nitrogen, and preparing a freezing microsphere; and (3) transferring the freezing microsphere into a freeze dryer, and performing freeze-drying on the freezing microsphere to obtain a freeze-drying detection reagent microsphere. A glucose detection reagent provided by the invention is a spherical granular freeze-drying reagent and can be pre-packaged into a detection chip. Compared with an existing liquid detection reagent, the glucose detection reagent has the advantages that the storage, transportation and the like at the stable room temperature can be realized. Compared with a freeze-drying powder reagent, the glucose detection reagent has the advantages of accuracy in quantification, convenience in packaging treatment and the like.
Description
technical field [0001] The invention relates to the field of freeze-dried microspheres and their preparation, in particular to a concentrated glucose detection reagent freeze-dried microspheres and a preparation method thereof. Background technique [0002] The sugar in the blood is primarily glucose, which is the form of sugar transported in the body. The fasting blood glucose concentration of normal people is 3.61-6.11mmol / L. Fasting blood glucose over 7.0mmol / L is called high blood sugar . Blood glucose concentration below 3.61mmol / L is called Hypoglycemia . Maintaining a constant level of blood sugar is extremely important for ensuring the normal functional activities of various tissues and organs of the human body, especially brain tissue. The level of blood sugar is closely related to the function of the human body. Therefore, the detection of blood sugar level is of great significance for clinical diagnosis and treatment. [0003] With the continuous progress o...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66
CPCG01N33/66
Inventor 张涛黎海文刘聪周武平蒋克明郭振
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Login to view more 
Login to view more