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Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof

A technology for detecting reagents and glucose, which is applied in the field of concentrated glucose detection reagent freeze-dried microspheres and its preparation, to achieve the effects of simplified operation steps, uniform size and shape, and easy packaging

Active Publication Date: 2014-12-10
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of concentrated glucose detection reagent freeze-dried microsphere and preparation method thereof, to solve the problems existing in prior art liquid and freeze-dried powder glucose detection reagent

Method used

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  • Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof
  • Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof
  • Freeze-drying concentrated glucose detection reagent microsphere and preparation method thereof

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preparation example Construction

[0042] A method for preparing concentrated glucose detection reagent freeze-dried microspheres, comprising the following steps:

[0043] (1), prepare glucose detection reagent;

[0044] (2), add lyoprotectant and plasticizer, filter to remove insoluble matter after fully dissolving, vacuum degassing removes air bubble;

[0045] (3) Micro-droplets are prepared by using a liquid separation system that can be accurately quantified, and the droplets are dropped into liquid nitrogen, and frozen reagent microspheres are formed after being cooled by liquid nitrogen;

[0046] (4) Transfer the frozen reagent microspheres into a lyophilizer to freeze-dry to prepare the detection reagent lyophilized microspheres.

[0047] The volume of the droplet in step (3) is precisely controlled by a liquid separation system, the size of the droplet is 2-10ul, and the size error is within 2%.

[0048] The glucose detection reagent freeze-dried microspheres that can be used for instant detection ins...

Embodiment 1

[0062] Embodiment 1 table 1, glucose detection reagent composition

[0063] Element

Embodiment 2

[0064] Embodiment 2 table 2, glucose detection reagent composition

[0065] Element

concentration

Triethanolamine buffer

150mM

ATP

6mM

NADP

6mM

Hexokinase

15KU / L

Glucose 6-phosphate dehydrogenase

6KU / L

Methyl hydroxymethylbenzoate

1g / L

polyethylene glycol 4000

30g / L

polyethylene glycol 80000

30g / L

[0066] Trehalose

10g / L

Surfactant Trion X-100

3g / L

[0067] The surfactant TrionX-100 in the glucose detection reagent can effectively control the generation of air bubbles during the dissolution of the reagent microspheres; the lyoprotectant in the reagent can be sucrose, trehalose, polyethylene glycol, bovine serum albumin, poly One or more of vinylpyrrolidone, mannitol, and dextran, the content of which can be determined according to the morphology and solubility of the freeze-dried microspheres of the detection reagent.

[0068] (2) Use th...

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Abstract

The invention discloses a freeze-drying concentrated glucose detection reagent microsphere and a preparation method thereof. The freeze-drying microsphere comprises a triethanolamine buffer solution, ATP, NADP, hexokinase, 6-glucose dehydrogenase phosphate, a preservative, trehalose, polyethylene glycol 8000, polyethylene glycol 20000, TrionX-100 and the like. The preparation method comprises the following steps: (1) preparing a solution, and performing filtration and degassing; (2) preparing droplets by using a precise quantification liquid separation system, dropwise adding the droplets into liquid nitrogen, and preparing a freezing microsphere; and (3) transferring the freezing microsphere into a freeze dryer, and performing freeze-drying on the freezing microsphere to obtain a freeze-drying detection reagent microsphere. A glucose detection reagent provided by the invention is a spherical granular freeze-drying reagent and can be pre-packaged into a detection chip. Compared with an existing liquid detection reagent, the glucose detection reagent has the advantages that the storage, transportation and the like at the stable room temperature can be realized. Compared with a freeze-drying powder reagent, the glucose detection reagent has the advantages of accuracy in quantification, convenience in packaging treatment and the like.

Description

technical field [0001] The invention relates to the field of freeze-dried microspheres and their preparation, in particular to a concentrated glucose detection reagent freeze-dried microspheres and a preparation method thereof. Background technique [0002] The sugar in the blood is primarily glucose, which is the form of sugar transported in the body. The fasting blood glucose concentration of normal people is 3.61-6.11mmol / L. Fasting blood glucose over 7.0mmol / L is called high blood sugar . Blood glucose concentration below 3.61mmol / L is called Hypoglycemia . Maintaining a constant level of blood sugar is extremely important for ensuring the normal functional activities of various tissues and organs of the human body, especially brain tissue. The level of blood sugar is closely related to the function of the human body. Therefore, the detection of blood sugar level is of great significance for clinical diagnosis and treatment. [0003] With the continuous progress o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66
CPCG01N33/66
Inventor 张涛黎海文刘聪周武平蒋克明郭振
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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