This invention discloses a foamed SiO2
enzyme array and its application in the preparation of
diglyceride edible oil, belonging to the field of oleochemical technology. This invention prepares a hydrophobic foamed SiO2 carrier (MCF-C8), and loads lipases ANL, NE, and CALB separately using a physical
adsorption method to prepare three functionally
specific enzyme arrays. Then, a dual-
coupling reaction strategy of "
hydrolysis-esterification" and "
glycerolysis-esterification" is constructed. ANL@MCF-C8 catalyzes the moderate
hydrolysis of
vegetable oil, or NE@MCF-C8 catalyzes the
glycerolysis of
vegetable oil, to obtain a primary
diglyceride mixture. The byproduct fatty acids produced in the reaction are recovered and esterified with
glycerol under the
catalysis of CALB@MCF-C8 to obtain a secondary
diglyceride mixture. After purification, an
edible oil product with a diglyceride content of 40%~82% and a 1,3-diglyceride content of 90% is obtained. This invention achieves efficient
recovery and utilization of byproduct fatty acids, improves product yield, and the
enzyme array has strong stability, is easy to separate and recover, and can be reused, effectively reducing production costs.