The application discloses a kind of
metal organic framework ZIF-8 fixed
enzyme and its preparation method and application in preparation of
diglyceride, to provide a kind of can effectively improve
enzyme stability and the reuse of fixed
enzyme, with the characteristics of simple operation, mild condition, can effectively promote
edible oil preparation rich in
diglyceride component
edible oil while retaining enzyme activity, its prepared
diglyceride edible oil byproduct is less,
active ingredient is higher;Its technical scheme is that
aspergillus niger
lipase is added to
zinc nitrate solution, is uniformly dispersed, then, slowly 2-methylimidazole solution is added, uniform speed stirring 60min, stationary 20min, the product is centrifuged, washed, freeze-dried to obtain fixed enzyme;Then
raw material oil and
glycerol are mixed, emulsified, then ZIF-8 fixed enzyme is added,
glycerolysis reaction is carried out at 40~70℃ for 2~12h, after reaction, fixed enzyme is centrifuged, liquid is purified by molecular
distillation, and DAG-rich edible oil is obtained;It is related to
food technology field.