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Method for extracting squalene from grease

A technology for squalene and oil, applied in chemical instruments and methods, purification/separation of hydrocarbons, hydrocarbons, etc., can solve problems such as large amount of organic solvent used, reduce the use of organic solvents, simple and effective operation, Environmentally friendly and efficient process

Active Publication Date: 2017-05-31
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to propose a highly efficient and environmentally friendly process for the technical problem of the large amount of organic solvent used in the extraction of squalene from oils and fats in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Take 10kg of olive oil (with a squalene content of 0.36%), add 5% lipase 435 and 5kg of glycerol, react in a water bath at 60°C for 8 hours, remove the lipase by centrifugation, and separate excess glycerol. Add 3L of water and 720g of β-cyclodextrin to the oil, stir in a water bath at 60°C for 1 hour, let stand at 4°C for 30 minutes, filter to obtain the clathrate, wash the clathrate with a small amount of water at room temperature, and use 95% ethanol to remove the clathrate Reflux at 80°C for 1 hour, filter while hot, concentrate the filtrate to obtain 46.5 g of crude squalene, use neutral alumina column to adsorb and separate, and elute with n-hexane to obtain 31.1 g of squalene, the content is 98.4%, the transfer rate 85.4%.

Embodiment 2

[0033]Example 2: Take 10kg of cod liver oil (squalene content 29.6%), add 15% lipase TL IM and 1.25kg glycerol, react in a water bath at 40°C for 18 hours, filter to remove lipase, and separate excess glycerol. Add 30L of water and 5.9kg of β-cyclodextrin to the oil, stir in a water bath at 50°C for 2 hours, let stand at 4°C for 30 minutes, filter to obtain the clathrate, wash the clathrate with a small amount of water at room temperature, and use 90% Ethanol was refluxed at 70°C for 1.5 hours, filtered while hot, and the filtrate was concentrated to obtain 3.22 kg of crude squalene, which was separated by adsorption and separation on a neutral alumina column to obtain 2.71 kg of squalene, with a content of 99.1% and a total transfer rate of 90.6%.

Embodiment 3

[0034] Example 3: Take 10kg of deep-sea fish oil (squalene content 13.6%), add 10% lipase RM IM and 10kg glycerol, react in a water bath at 50°C for 12 hours, filter to remove lipase, and separate excess glycerol. Add 20L of water and 6.8kg of β-cyclodextrin to the oil, stir in a water bath at 60°C for 1.5h, let stand at 8°C for 60min, filter to obtain the clathrate, wash the clathrate with a small amount of water at room temperature, and use 80 % ethanol was refluxed at 90°C for 30 minutes, filtered while it was hot, and the filtrate was concentrated to obtain 1.51 kg of crude squalene, which was separated by adsorption on a neutral alumina column to obtain 1.23 kg of squalene, with a content of 97.9% and a total transfer rate of 88.4%.

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PUM

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Abstract

The invention discloses a method for extracting squalene from grease. The method comprises the following steps of using glycerin and lipase to perform glycerolysis on the grease; converting most of triglyceride in the grease into monoglyceride and diglyceride; utilizing a cyclodextrin inclusion technique to extract the squalene, so as to avoid the complicated operation of the one-step column chromatography or saponification extraction method, greatly reduce the usage amount of organic solvent, and avoid the defects of high cost and low efficiency in the supercritical extraction and high-efficiency counter-current chromatography. The method has the advantages that the amount of impurities is small, the squalene and residual sterol are adsorbed and separated by an alumina column, and the purpose of further purifying the squalene is realized.

Description

technical field [0001] The invention belongs to the field of chemical engineering, and in particular relates to a method for extracting squalene from oil. Background technique [0002] Squalene is also known as squalene, tricosahexaene, cod liver oil terpene, and its chemical name is 2, 6, 10, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-twenty Tetracarbahexaene, a highly unsaturated hydrocarbon compound, was first discovered in black shark liver oil by Japanese chemist Tsujimoto in 1906. Squalene is a colorless oily liquid at normal temperature, insoluble in water, hardly soluble in methanol, ethanol and glacial acetic acid, easily soluble in organic solvents such as ether, petroleum ether, acetone, carbon tetrachloride, etc., polarity and triglyceride Esters and sterols are close. [0003] Squalene is often used as the base material of medical ointments (hydrophilic ointment, water-absorbing ointment), and also used as a component of suppositories, which can promote the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C7/00C07C7/148C07C7/152C07C7/04C07C7/12C07C11/21
CPCC07C7/005C07C7/04C07C7/12C07C7/148C07C7/152C07C11/21Y02P20/54
Inventor 许文东蔡鸿飞唐顺之牟肖男袁诚关伟键王小妹李继荣杨玉琼熊淑娴江程王国财吴伟锋
Owner GUANGZHOU HANFANG PHARMA
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