Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones

An aerobic ammonia oxidizing bacteria, acyl homoserine technology, applied in chemical instruments and methods, water/sludge/sewage treatment, biological water/sewage treatment, etc. AOB and quorum sensing less problems, to achieve the effect of efficient and stable operation, reduce loss, and shorten the required time

Pending Publication Date: 2015-11-25
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, there are few studies on AOB and quorum sensing, and no research on the intrinsic relationship between AHLs structure and AOB aggregation growth has been found.

Method used

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  • Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones
  • Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones
  • Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones

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Experimental program
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Effect test

Embodiment 1

[0025] Inorganic ammonia nitrogen (NH 4 Cl) The artificial wastewater domesticates the activated sludge with AOB as the single dominant bacterium in the SBR reactor. After the domestication, the inorganic ammonia nitrogen volume load of the sludge reaches 0.81g / (L·d), and the ammonia nitrogen degradation efficiency is close to 100%. The average particle size of the mud is 175.03 μm, and the flora structure is AOB as the single dominant bacterium (content is 25%). 5mlOD 600 =0.1 bacterial suspension was added to the microwell plate, and nitrogen source (100mgNH 4 + -N / L), trace elements and 2μM 3-oxo-C 6 -HSL, after being placed in an incubator (28°C) for 24h and 48h respectively, the biomass of the biofilm was measured. The 24h in this embodiment means that the microwell plate is put into the incubator and then continuously cultured, and the biomass of the biofilm is taken out after 24h; 48h means that the nitrogen source, trace elements and 3-oxo of the same load are adde...

Embodiment 2

[0028] Inorganic ammonia nitrogen (NH 4 Cl) The artificial wastewater domesticates the activated sludge with AOB as the single dominant bacterium in the SBR reactor. After the domestication, the inorganic ammonia nitrogen volume load of the sludge reaches 1.5g / (L·d), and the ammonia nitrogen degradation efficiency is close to 100%. The average particle size of the mud is 168.5 μm, and the flora structure is AOB as the single dominant bacterium (content is 40%). 5mlOD 600 =0.2 bacteria suspension was added into the microwell plate, and nitrogen source (100mgNH 4 + -N / L), trace elements and 2μM AHLs with different chemical structures were placed in an incubator (35°C) for 12h and 24h respectively, and then the biomass of the biofilm was measured to investigate the effect of the chemical structure of AHLs on the ability of AOB to attach and grow . The AHLs selected in this embodiment are respectively C 6 -HSL, C 8 -HSL, C 10 -HSL and 3-oxo-C 6 -HSL, 3-oxo-C 8 -HSL, 3-oxo...

Embodiment 3

[0031] The activated sludge (60% content) in which AOB is the dominant bacterium was cleaned and then inoculated into three identical columnar reactors (effective volume 200ml, height 160mm, inner diameter 40mm). The initial inoculated sludge concentration was 2.36gMLSS / L, MLVSS1.87g / L, and the ammonia nitrogen load was 0.4kg / (m 3 d). Sequenced batch operation is carried out. A cycle of sequenced batch operation includes water intake, aeration, sedimentation and drainage stages, and the hydraulic retention time is 24h. The influent of one of the reactors only contained nitrogen source and trace elements, and no AHL signal molecules were added. In addition to nitrogen source and trace elements, the influent water of the other two reactors also added 3-oxo-C 6 -HSL and C 6 -HSL. During the aeration phase, keep DO>2mg / L and pH between 7-8. After first settling for 1-10 minutes, drain the loose and slow-settling sludge on the upper layer, then settle for 20-30 minutes, and the...

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Abstract

The invention belongs to the technical field of sewage treatment and relates to a method for enhancing aerobiotic ammonia oxidizing bacterium (AOB) gathering through N-acylated homoserine lactone (AHLs) compounds. The method includes the specific steps that AHLs signal molecules with the specific variety and the specific concentration are added to a reactor containing AOB activated sludge, under the condition that a sufficient nitrogen source and oxygen are provided, a quorum-sensing system is enhanced, aerobiotic ammonia oxidizing bacterium gathering growth is promoted, and biological membranes or granular sludge is obtained. According to the method, the AOB physiology is adjusted and controlled on the aspect of the signal molecules, AOB gathering growth is enhanced, rapid and efficient enrichment of AOB is achieved, and the AOB is fixed in a qualified sense, so that losses are reduced, and time needed for AOB enrichment is shortened. A new thought is provided for rapid AOB enrichment through the method, the method is expected to be used for the biological nitrogen removal process, and important significance is provided for efficient and stable operation of the ammonia-nitrogen wastewater biological treatment process.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for strengthening the aggregation of aerobic ammonia oxidizing bacteria by using N-acyl homoserine lactone compounds. Background technique [0002] The discharge of high-concentration ammonia nitrogen wastewater without proper treatment will cause eutrophication of the water body, aggravate the shortage of water resources and the current situation of water pollution. At present, denitrification of wastewater takes a long time and the process is complicated. The nitrogen content of treated wastewater often exceeds the discharge standard. How to effectively denitrify wastewater has become an increasingly urgent problem. Biological denitrification has strong advantages due to low cost and no pollution, and nitrification reaction is the rate-limiting step. Aerobic ammonium oxidizing bacteria (AOB) play an irreplaceable role in the nitrification reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 李安婕侯保连李美茜吴玲娟陈舒涵
Owner BEIJING NORMAL UNIVERSITY
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