198 results about "Microwell Plate" patented technology

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention discloses a design method of an array type silencer adaptive to a main ventilation air path between a stator iron core of a hydraulic generator and an air cooler and the array type silencer, and relates to the technical field of suppression or reduction of noise of the hydraulic generator. The method comprises the steps of S1, obtaining aerodynamic noise data and airflow parameters of a main ventilation air path, S2, determining a silencing thought and a micro-perforated plate array core structure according to broadband vortex, discrete rotation, vortex falling and surge noise, S3, determining the boundary dimension and preliminarily calculating the airflow parameters, S4, designing plate parameters in combination with a micro-perforated resonance formula, and S5, calculating the noise of the micro-perforated plate array. S5, performing simulation optimization and S6, accurately accounting airflow parameters, and locking a scheme meeting requirements; the silencer comprises a shell and a plurality of micro-perforated plates, and the multiple layers of micro-perforated plates are arranged in series. The invention has the advantages of no sound absorption material, low wind resistance (the air volume attenuation is less than or equal to 5%, and the pressure loss is less than or equal to 200Pa), the noise of the main wind path is reduced by 5-7dB (A), the service life is long, the operation and maintenance cost is low, and the requirements of the main wind path are met.

The invention provides an ultrathin broadband sound absorption composite structure which comprises a plurality of silencing units, every two of the silencing units are in contact with each other on the same plane to form the three-dimensional ultrathin broadband sound absorption composite structure, each silencing unit comprises a shell, a through hole is formed in each shell, and the through holes are communicated with the silencing units. A thin film is arranged in the position, located in the center of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, a micro-pore plate is arranged in the position, located on the periphery of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, and the micro-pore plate or the thin film or other resonance structures are arranged in the micro-pore plate or the thin film or other resonance structures in the micro-pore plate or the thin film or other resonance structures. The ultra-thin broadband sound absorption composite structure provided with the micropore plate and the thin film is small in size, the thickness of the ultra-thin broadband sound absorption composite structure is 1 / 23 of the sub-wavelength size, the ultra-thin broadband sound absorption composite structure has the ultra-thin characteristic, the sound absorption frequency band is wide, the noise reduction effect is good, and the ultra-thin broadband sound absorption composite structure can be suitable for sound absorption and noise reduction of various small places and spaces.

The invention relates to the technical field of power equipment cooling, and discloses a reactive power compensation device evaporative cooling system which comprises an SVG compensation cabinet, one side of the SVG compensation cabinet is provided with a cooling liquid discharge pipe and a cooling liquid access pipe, and the cooling liquid discharge pipe and the cooling liquid access pipe are both connected to a heat exchange mechanism. An automatic replacement mechanism is arranged in the heat exchange mechanism, and the heat exchange mechanism is connected to the cooling mechanism; according to the invention, the heat exchange mechanism is connected with the micro-pore plate of the IGBT module for heat exchange, meanwhile, the cooling mechanism is matched for evaporative cooling of cooling water for heat exchange, and the automatic replacement mechanism can complete the replacement process of the filtering tank under the condition of continuous work without shutdown, so that stable operation of power equipment is guaranteed; meshes of the air inlet plate are cleaned through the fan cover anti-blocking mechanism, so that the meshes are prevented from being blocked; and in the cleaning process, the circulating cleaning mechanism is matched to automatically remove impurities such as fallen leaves in the cooling mechanism, and the labor cost of system management is reduced.

The invention provides a rotational flow multi-channel composite gas-liquid separation device and a separation method. The separation device comprises a shell assembly, and a gas inlet cavity, a transition cavity, a filter cavity and a discharge cavity which are separated and communicated in the shell assembly; an umbrella-shaped cover, an inner curved surface fin assembly, an outer curved surface fin assembly and a scroll plate are arranged in the air inlet cavity, and a liquid outlet is formed in the corresponding shell assembly below the scroll plate; the transition cavity is arranged above the side of the air inlet cavity, the filtering cavity is located on the side of the air inlet cavity, the upper end of the filtering cavity is communicated with the transition cavity, a micropore plate is arranged in the filtering cavity, a cavity, corresponding to the lower portion of the micropore plate, of the filtering cavity is communicated with the discharging cavity, an inlet of the exhaust port is communicated with the discharging cavity, and an outlet of the exhaust port is communicated with the outside. The device is reasonable in structural design, adopts an integrated design, adapts to the spatial layout of the electric vehicle, can effectively improve the separation efficiency, is reliable in liquid drainage, can effectively avoid reflux and gas leakage, and is high in working condition adaptability.

Furniture such as desks and other items are provided with sound absorbing features consisting of micro-perforations extending through structures having vertical surfaces. The micro-perforations are formed using a LASER cutting tool or a punch and are either cylindrical or frustoconical. The micro-perforations are formed in drawer facings, doors, support structures, and free-standing objects and storage devices having vertical surfaces. The micro-perforations have diameters in the range of 0.20 to 0.70 mm. The micro-perforations have been found to increase sound absorption of a desk by as much as 4 Sabins within the frequency range of 100 to 5,000 Hz. Structural panels consisting of front and rear micro-perforated panels surrounding a porous central core may be employed in such furniture.

The utility model relates to the technical field of micro-pore plates, in particular to an improved shallow-pore pore plate which comprises a rotating structure used for improving the mounting efficiency and a pore plate body used for bearing the pore plate, a hole is formed in one side of the pore plate body, the rotating structure is mounted in the hole, and a base is fixedly connected to the bottom of the pore plate body. Through the arrangement of the rotating structure which comprises the fixing rod, the gasket, the circular ring, the square block, the clamping rod, the groove, the eccentric wheel, the supporting rod, the nut and other components, the rotating structure can drive the clamping rod to move in the groove by rotating the eccentric wheel through the mutual matching relation between the eccentric wheel component and the clamping rod component, and therefore the position of the clamping rod is adjusted; the pore plate body can be quickly mounted and dismounted through the rotating structure, the problems that a traditional pore plate is low in mounting efficiency and inconvenient to dismount are solved, and the mounting and dismounting efficiency of the pore plate is improved.

A fiber optic device and method for uniform illumination of biological samples distributed over one or more microcell arrays of a microwell plate can establish more reliable polymerase chain reaction (PCR) data sampling. In some embodiments, the apparatus includes a light engine that emits a light beam captured by a fiber optic bundle for delivering one or more light beams to each microchamber; each light beam thereby fluoresces the biological sample. The fiber optic cable of the fiber optic bundle comprises a distal output end held in place using a mechanical support for flexibly aligning the output beam of the distal output end to be arranged such that the center of the output beam is directed to different locations in the one or more microcell arrays, wherein each of adjacent ones of the output beams partially overlaps so that the light distribution is uniform.

1. The name of the design product: microplate reader. 2. The use of the design product: microplate reader is used for sample measurement analysis, and is used for reading various information contained in the microplate. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view. 5. Other changes in the state of the case need to be explained: each reference figure shows the light-emitting state of the progress bar panel when working in the microplate reader.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

A microplate stacker includes a housing having a microplate port at a first end of the housing and a confinement plane at a second end of the housing. A plurality of screws are disposed substantially orthogonal to the microwell plate and the confinement plane, and the plurality of screws are disposed between the microwell plate and the confinement plane, where each screw has a thread. A drive motor is provided in the housing for rotating each of the plurality of screws at substantially the same rotation rate and substantially the same rotation direction. A transmission is provided in the housing for coupling the drive motor to each of the plurality of screws.

The invention relates to a solid fuel combustion characteristic test technology, and aims to provide a focusing single particle suspension variable atmosphere ignition combustion experiment device and method. A second microporous plate and a feed hopper are arranged at the top of a combustion chamber main body of the device, and a first microporous plate and a gas buffer cavity are arranged at the bottom; the two laser emitters are arranged in an included angle shape, and the energy of a single laser beam is lower than a fuel particle ignition threshold value; the two paths of laser intersect in the central area of the cavity to form a space energy focus, and the superposed energy of the two beams of laser can be absorbed at the position to reach an ignition threshold value; and light paths of diagnosis equipment in the optical acquisition module are intersected in a focus area. According to the invention, through a strategy of airflow disturbance and microwell plate blocking, particles completely move in airflow; the ignition behavior has spatial selectivity, the success rate is high and controllable, and the research is closer to the actual application scene. Through the design of single-particle fixed-point triggering, the system complexity and the control difficulty can be reduced, and the operation stability and the experiment repeatability can be improved.

An organoid probing system includes a probe head having a probe, a first stage along which the probe head is movable in an X direction, and a second stage configured to move a microwell plate in a Y direction. The microwell plate has a microwell containing an organoid. The probe head is movable in a Z direction to insert the probe into the microwell or retract the probe away from the organoid in order to measure a property of the organoid.

The industrial design sought to be registered is primarily a well plate for laboratory use, covering the front, back, right and left sides and featuring a peripheral ridge structure interrupted by the four corners and the back. 1.1) Front view; 1.2) Back view; 1.3) Right side view; 1.4) Left side view; 1.5) Top view; 1.6) Bottom view; 1.7) Perspective view

An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.

The invention discloses a digoxin drug concentration detection method, which comprises: S1, determining a to-be-detected drug, and carrying out working curve preparation on a detection object, the to-be-detected drug being digoxin; s2, taking out the to-be-detected sample, the calibration product, the quality control product and the internal standard extraction liquid, placing at room temperature, and uniformly mixing; s3, respectively taking a to-be-detected sample, a calibration product, a quality control product and a blank sample, putting into an EP tube, adding the internal standard intermediate solution, adding methanol, uniformly mixing by vortex, and centrifuging; s4, putting the supernate into a 96-well plate, adding ultrapure water, and putting the 96-well plate on a microwell plate oscillator for shaking; and S5, centrifuging in a multi-tube rack automatic balancing centrifuge, detecting and verifying by using liquid chromatogram-tandem mass spectrum, and collecting a mass spectrum signal. According to the present invention, the high performance liquid chromatography separation and the mass spectrometry are combined, such that the complex background interference in the blood sample is overcome, the cost performance is improved, and the high sensitivity, the high accuracy and the convenient detection can be achieved for the digoxin sample with the low in-vivo concentration.

The invention provides a device and a method for measuring a high-speed bottom flow velocity field based on micropore injection. The device comprises a power source, a particle storage container, a model supporting piece and a model, the power source is communicated with the particle storage container and is used for providing power for injection of the tracer particles; one end of the model supporting piece extends into the model and is used for supporting the model, the other end of the model supporting piece is connected with the particle storage container, a ventilation pipeline is arranged in the model supporting piece, and tracer particles can be injected into the model along the ventilation pipeline under the action of power provided by the power source; a first cavity and a second cavity which are sequentially communicated with the ventilation pipeline are formed in the model, a first microwell plate is installed between the first cavity and the second cavity, and a second microwell plate is installed at the end, away from the first cavity, of the second cavity. According to the device and the method, through development of a bottom flowing space device, supplement of bottom tracer substances is achieved, and the problem that high-speed bottom flowing tracer substances are difficult to enter is solved.

This utility model relates to the field of screening equipment technology, and more particularly to a screening device for covalent inhibitor reactions. It includes a screening device body, on the inner wall of which a microwell plate is installed. Four foot pads are installed on one side of the screening device body. An adjustment mechanism is provided at one end of the screening device body. The adjustment mechanism includes two fixing plates, each fixedly connected to one end of the screening device body. A motor is fixedly connected to one side of each fixing plate, and a gear is fixedly connected to the output end of the motor. The gear teeth mesh with a rack. This utility model provides a covalent inhibitor reaction screening device that allows the microwell plate to be placed in the screening device by means of the adjustment mechanism, replacing manual labor. The adjustment mechanism improves the stability of microwell plate placement, reduces manpower consumption, increases laboratory efficiency, and enhances experimental accuracy.

The application discloses an automatic mobile line carrier secondary positioning structure, a fixed base is used for supporting a movable top block, the fixed base is installed on a loading station of the automatic mobile line, a support platform is used for bearing a planar micro-hole plate, when the automatic mobile line drives the support platform to translate along a straight line to the loading station, the movable top block and the support platform are mutually inserted and positioned through at least two groups of wedge-shaped positioning blocks and wedge-shaped positioning grooves, secondary positioning of the support platform and the planar micro-hole plate placed thereon is realized; elastic members are arranged between the fixed base and the movable top block, when the support platform contacts the movable top block, the movable top block compresses the elastic members, a buffering effect is achieved, and damage is avoided; through the arrangement of the movable top block, the support platform and the planar micro-hole plate are accurately positioned, and accurate drop of liquid drops can be ensured.

The application discloses a kind of transfer factor detection equipment and method, it is related to biological detection technical field, including microplate reader, detection chamber, microporous plate, detection component, the inner wall of the detection chamber is symmetrically provided with chute, and the inner wall of chute is connected with tray with sliding, the bottom of the tray is provided with push positioning mechanism, to in push detection, the detection position of the microporous plate is fixed with adhesion.This kind of transfer factor detection equipment, in push detection process, can be rotated by driving tray to drive gear, using the cooperation of guide groove and shaft, four positioning blocks are synchronously moved inward, so that four positioning blocks can be used to adhere positioning four sidewalls of microporous plate when being pushed to detection position, guarantee the accuracy of microporous plate position in detection process, in turn guarantee the accuracy of subsequent detection result of the equipment.

The application discloses a kind of Gui Shiwei Chinese herbal medicine organophosphorus pesticide rapid detection method and detection kit, by the sample of Gui Shiwei medicine powder to be measured is extracted by acetonitrile solution containing acetic acid, salting-out stratification is taken supernatant;With colloidal gold labeled anti-organophosphorus pesticide small peptide antibody incubation, carry out competition immune response in the microwell plate coated with organophosphorus hapten-protein complex;After washing, the absorbance is determined at 450 to 550nm wavelength, calculate competition inhibition rate and compare with preset first threshold value to determine whether it is over standard;The method is simple and rapid;In addition, for the detection needs of different organophosphorus pesticides, by setting different organophosphorus hapten-protein complex coating area in the microwell plate, the simultaneous detection of multiple organophosphorus pesticides is realized, and the applicability of organophosphorus pesticide residue detection is further improved.

The utility model discloses a combined microwell plate, which comprises a microwell plate body, a plurality of sample tubes are arranged on the microwell plate body, a shielding ring is movably arranged in each sample tube, the plurality of shielding rings are fixedly connected with a mounting plate, an insertion block is arranged at the lower end of the mounting plate, the insertion block is L-shaped, and the insertion block is fixedly connected with the mounting plate. The number of the insertion blocks is two, two fixing chambers matched with the insertion blocks are arranged on the microwell plate body, and fixing assemblies are arranged in the fixing chambers, so that the phenomenon of liquid splashing in the oscillating and mixing process is prevented, cross infection is avoided, the accuracy of a sample detection result is improved, and the success rate of sample analysis and test is guaranteed; meanwhile, when the liquid is dumped and cleaned, the mounting plate and the shielding ring can be detached, so that the sample tube is convenient to clean.

The invention discloses a gas phase compensation compressible fuel injection coupling test device and a correction method, the gas phase compensation compressible fuel injection coupling test device comprises a piston pressurization cavity, an injection rule test cavity, a connecting cylinder and an elastic modulus test cavity which are coaxially communicated, and a driving / driven piston is in rigid linkage to form equivalent specific volume coupling; the jet cavity is connected with the sound velocity testing cavity through a communication channel, a microwell plate and a variable resonant cavity are arranged in the channel to form an adjustable acoustic filter network, and the sound velocity testing cavity is provided with sound velocity ports which are oppositely arranged up and down. The system is triggered by a unified hardware clock and synchronously acquires pressure, displacement, temperature and sound velocity. According to the method, the volume elastic modulus is measured firstly, the injection rate is obtained according to a volumetric method, the gas content and the actual elastic modulus are inversed in combination with the cross-correlation sound velocity and the Wood equation, temperature correction is introduced, and real-time compensation of the injection rule is achieved through expansion / unscented Kalman filtering online estimation back substitution. According to the scheme, high-frequency interference is inhibited, sampling time difference is avoided, and injection rate metering accuracy is improved.

A method of manufacturing a microplate including a plurality of wells includes obtaining an upper portion of the microplate. A bottom surface of the microplate is coated with a bottom surface uncured resin. A sheet material is disposed adjacent a central portion of the bottom surface. A frame is disposed adjacent an edge portion of the bottom surface. The frame contains a frame uncured resin. At least a portion of the bottom surface uncured resin is cured to produce a bottom surface cured resin. At least a portion of the frame uncured resin is cured to produce a frame cured resin. A remaining portion of the bottom surface uncured resin is removed subsequent to curing at least a portion of the bottom surface uncured resin.

This utility model relates to the field of drug analysis technology, specifically a rotary centrifuge-based drug property analysis and testing device. It includes a centrifuge body with a rotating shaft inside. A top cover is rotatably connected to the top of the centrifuge body. An installation component is provided on the outer wall of the rotating shaft, and a placement component is provided on the side wall of the installation component. The advantages are: by using the installation component and the placement component in conjunction, the sample can be directly placed into the microplate and vertically inserted into the limiting groove to complete the installation. Rotating to open the fixed cover allows the rotating squeezing block to press the slider, thus disassembling the microplate. This reduces the number of operation steps, supports various sizes of microplates, and solves the problems of inconvenient microplate replacement and cumbersome operation in traditional centrifuges. It not only improves operational convenience and experimental efficiency but also enhances safety, compatibility, and scalability.

The application discloses a microwell plate assembly and application thereof. The microwell array plate assembly comprises a bottom plate provided with a plurality of microwells, the inner wall and bottom of the microwells are provided with a hydrophilic coating, and the top end is provided with a hydrophobic coating; a top plate capable of being fixed to the bottom plate, and the side of the top plate facing the bottom plate is provided with a hydrophobic coating. The application is provided with a hydrophobic coating on the side of the top plate facing the bottom plate, a hydrophilic coating on the inner wall and bottom of the microwells of the bottom plate, and a hydrophobic coating on the top end, water phase medium can be used to infiltrate the microwells, oil phase medium can be used to seal the microwells, the microwells can be controllably sealed and opened, single cells and the like can be effectively captured and treated, cell free interferences can be prevented, meanwhile, the microwells can be repeatedly sealed and opened through replacement of the water phase medium and the oil phase medium, reagents can be replaced and added, and the like, and the operation is convenient.

The invention relates to the technical field of microbial screening, in particular to a method for high-throughput screening of flavone-transformed bacteria and a screening system thereof. The method comprises the following steps: firstly, inoculating a to-be-detected strain into a basal culture medium containing a flavone substrate for fermentation, then scanning fermentation broth at two different time points by using a full-wavelength microplate reader through a quartz microplate, calculating an absorbance difference value at at least one preset wavelength, and finally, calculating the absorbance difference value at least one preset wavelength. The strains with the absorbance difference larger than a preset threshold value are screened as primary screening positive strains. Compared with the traditional technology, the method has the advantages that the screening efficiency is improved by hundreds of times, the sample consumption is reduced by 75%, the industrial bottleneck that multi-structure flavonoids need to be repeatedly developed is broken through, and an innovative tool is provided for microbial resource development.

The application discloses a high-throughput screening method of phenylethanolamine-N-methyltransferase. Substrate norepinephrine and S-adenosyl methionine, PBS buffer, S-adenosyl-L-homocysteine hydrolase SAHH are added in a 96-well plate of a microplate reader, enzyme sample phenylethanolamine-N-methyltransferase is added, in a microplate shaking reactor, 20-50 DEG C, 500-1000 rpm, reaction for 1-3 h, 4 DEG C, 3700 rpm centrifugation, the supernatant is taken, compound NDCC is added again, reaction for 0-90 min, during the reaction, the microplate is taken out every 1-30 min, and the microplate reader is used to detect the absorbance value; according to the absorbance value change, the enzyme activity is defined as the change of OD405nm absorbance value per minute * 1000000 under specific conditions. The application has the characteristics of simple operation, low cost and accurate detection, and has far-reaching significance for the directed evolution of phenylethanolamine-N-methyltransferase.