341 results about "Microwell Plate" patented technology

A temperature regulation system for samples in a microplate includes a housing defining an interior volume and first and second airflow ports defined by a first and second exterior surfaces of the housing. The system includes an electromagnetic mixing system that has a base plate defining a plurality of openings, and a plurality of electromagnets. Each electromagnet defines an axis that extends substantially vertically from the base plate. The base plate is disposed in the interior volume and each of the plurality of electromagnets extend toward the first airflow port. A fan is disposed in the interior volume substantially below the base plate. Activation of the fan draws air into the first or second airflow port, substantially parallel to each axis of the plurality of electromagnets, through the plurality of openings, and out of the other of the second and first airflow port.

A microplate includes a cultivation well, a feeding well, and at least one separation channel there between. The cultivation well, feeding well, and the separation channel each contain a first solution, and the cultivation well contains a subject organoid. A method of using such a microplate includes sealingly engaging a pipette with the feeding well. At least some of the first solution is aspirated into the pipette while maintaining the seal between the pipette and the feeding well. This causes the aspirated portion of the first solution to flow from the cultivation well, through the separation channel, and into the feeding well. A second solution is injected from the pipette while maintaining the seal between the pipette and the feeding well. The injected second solution flows from the feeding well, through the at least one separation channel, and into the cultivation well.

The invention discloses a KLHL11 antibody high-precision detection method based on an enzyme-linked immunosorbent assay. The detection method comprises the following steps: preparing a microwell plate pre-coated with an anti-human KLHL11 antibody, dividing micropores into a plurality of detection groups, and adding KLHL11 protein or peptide fragment solutions with different concentrations, a to-be-detected solution with unknown concentration and a contrast solution into each detection group. The detection precision of a current detection instrument, the operation capability level of an operator and the reliability of the current detection instrument and the operation capability level are evaluated by executing enzyme-linked immunosorbent assay operation steps including sample filling, incubation, washing, color development and optical density measurement, obtaining the optical density value of a detection group and calculating the mean value, the standard deviation and the variation index of each group. By establishing a standard curve of optical density and concentration, the concentration of an unknown sample can be calculated, and the performance of an instrument in different concentration ranges can be verified. In addition, the problems of drifting and cross contamination of the instrument are further detected by setting a detection group with a relatively large time interval and a high-low concentration gradient experiment.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention relates to the technical field of biological detection, and provides a kit and a method for rapidly detecting a platelet antibody by utilizing a phytolectin solid-phase adsorption method. The detection kit provided by the invention comprises a reaction plate which is coated with phytolectin and adsorbs platelets. According to the method, the platelet of multiple persons is adsorbed by utilizing the non-specific adsorption capacity of the phytolectin, and the adsorbed platelet is directly freeze-dried to the bottom of a microwell plate. During use, a detection result can be obtained only by adding detected plasma / serum into the reaction plate for incubation and then adding an anti-human globule reagent and indicating red blood cells, so that intermediate links of detection are reduced, and standardization and automation of detection are facilitated.

The invention relates to the technical field of microplate readers, and discloses a multifunctional microplate reader which comprises a full-wavelength microplate reader body, a detection cavity used for detecting a sample is formed in the full-wavelength microplate reader body, and a microplate used for placing the sample is arranged in the detection cavity. The full-wavelength microplate reader body is rotationally connected with a cover plate for sealing the detection cavity through a rotating shaft, the full-wavelength microplate reader body is further provided with a control panel for controlling the device to be opened and closed, a sample taking and placing assembly is arranged in the detection cavity, and a cleaning assembly is arranged on the sample taking and placing assembly. And a wiping assembly is arranged on the microporous plate. The device effectively avoids the problem that the accuracy of a sample detection result is affected due to the fact that an existing device is difficult to clean the surface of the microporous plate, and is high in practicability.

The invention discloses an intelligent detection and analysis system for biological evaluation data of a medical instrument, and relates to the technical field of data processing, and the system comprises an image acquisition module which carries out the rapid imaging of the initial physical planking state of cells in a microwell plate; the image processing and feature quantification module is used for identifying the cell object and extracting a plurality of physical operation quality (POQ) feature parameters representing a population space distribution mode of the cell object; the quality evaluation and early warning module is used for comparing the POQ fingerprint with a preset quality baseline in real time and generating an early warning signal when the deviation exceeds a threshold value; and the data management and trend analysis module is used for continuously recording and analyzing POQ fingerprint historical data associated with the automatic pipetting equipment. According to the method, the physical operation quality is monitored at the experiment starting point, so that the accuracy, the reliability and the experiment efficiency of biological evaluation data of medical instruments are improved, the resource waste and the failure risk caused by initial inoculation errors are effectively reduced, and a way is provided for intelligent management of automatic equipment.

The invention discloses a design method of an array type silencer adaptive to a main ventilation air path between a stator iron core of a hydraulic generator and an air cooler and the array type silencer, and relates to the technical field of suppression or reduction of noise of the hydraulic generator. The method comprises the steps of S1, obtaining aerodynamic noise data and airflow parameters of a main ventilation air path, S2, determining a silencing thought and a micro-perforated plate array core structure according to broadband vortex, discrete rotation, vortex falling and surge noise, S3, determining the boundary dimension and preliminarily calculating the airflow parameters, S4, designing plate parameters in combination with a micro-perforated resonance formula, and S5, calculating the noise of the micro-perforated plate array. S5, performing simulation optimization and S6, accurately accounting airflow parameters, and locking a scheme meeting requirements; the silencer comprises a shell and a plurality of micro-perforated plates, and the multiple layers of micro-perforated plates are arranged in series. The invention has the advantages of no sound absorption material, low wind resistance (the air volume attenuation is less than or equal to 5%, and the pressure loss is less than or equal to 200Pa), the noise of the main wind path is reduced by 5-7dB (A), the service life is long, the operation and maintenance cost is low, and the requirements of the main wind path are met.

The invention discloses reflow soldering equipment and process for SMT patch production. The reflow soldering equipment comprises a furnace body base, a conveying unit, a hearth and a sealing top cover. A conveying unit is horizontally laid on the furnace body base, the hearth is installed on the upper end face of the furnace body base in a sealed mode, and the interior of the hearth is evenly divided into three independent working areas which are the preheating area, the backflow area and the cooling area in sequence from left to right. A visual inspection chamber is installed at the position, located at the feeding end of the hearth, of the upper portion of the furnace body base. A heating device is arranged in each working area of the hearth; in the heating device of the backflow area, each microplate is slidably provided with a vertically arranged hot spray pipe, and the hot spray pipes can perform hot air pulse direct blowing on large-size surface-mounted elements on a PCB, directly heat pin welding spots, realize micro-swing attachment of the elements in cooperation with surface tension of solder paste, and eliminate mechanical stress when the chip mounter is placed.

The invention provides a molecular modification design method for improving esterification activity of lipase. The method comprises the following steps: calculating a channel structure of lipase to be modified through CAVER ANALYST 2.0, determining a modification site through comprehensive measurement of other calculation tools, establishing a mutant library by adopting NNK degenerate codons for site-saturated mutation, and screening out excellent mutants with improved esterification performance by adopting a microwell plate screening method based on a copper soap complexing method. According to the candida antarctica lipase B mutant M5 disclosed by the invention, in various different types of esterification reactions, the catalytic efficiency of the mutant M5 is improved by 4.5 times or more than that of a wild type. The molecular modification design method disclosed by the invention can be used for improving the esterification performance of the lipase and has relatively wide esterification reaction application applicability, so that the lipase mutant can play an important role in industrial application in the fields of foods, medicines or biodiesel and the like.

The invention provides an ultrathin broadband sound absorption composite structure which comprises a plurality of silencing units, every two of the silencing units are in contact with each other on the same plane to form the three-dimensional ultrathin broadband sound absorption composite structure, each silencing unit comprises a shell, a through hole is formed in each shell, and the through holes are communicated with the silencing units. A thin film is arranged in the position, located in the center of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, a micro-pore plate is arranged in the position, located on the periphery of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, and the micro-pore plate or the thin film or other resonance structures are arranged in the micro-pore plate or the thin film or other resonance structures in the micro-pore plate or the thin film or other resonance structures. The ultra-thin broadband sound absorption composite structure provided with the micropore plate and the thin film is small in size, the thickness of the ultra-thin broadband sound absorption composite structure is 1 / 23 of the sub-wavelength size, the ultra-thin broadband sound absorption composite structure has the ultra-thin characteristic, the sound absorption frequency band is wide, the noise reduction effect is good, and the ultra-thin broadband sound absorption composite structure can be suitable for sound absorption and noise reduction of various small places and spaces.

The invention discloses a plate-formwork composite sound absorption structure and a building wall surface of a transformer substation. Relates to the field of sound absorption structures, and the structure comprises a microwell plate which is provided with a plurality of first through holes; a plurality of second through holes are formed in the microporous film, the microporous film is attached to the microporous plate, the second through holes are smaller than or equal to the first through holes, and the second through holes are communicated with the first through holes. According to the invention, the technical problem of low use flexibility of the sound absorption structure caused by the sound absorption effect of the sound absorption structure based on the microplate in the related art is solved.

The invention relates to the technical field of power equipment cooling, and discloses a reactive power compensation device evaporative cooling system which comprises an SVG compensation cabinet, one side of the SVG compensation cabinet is provided with a cooling liquid discharge pipe and a cooling liquid access pipe, and the cooling liquid discharge pipe and the cooling liquid access pipe are both connected to a heat exchange mechanism. An automatic replacement mechanism is arranged in the heat exchange mechanism, and the heat exchange mechanism is connected to the cooling mechanism; according to the invention, the heat exchange mechanism is connected with the micro-pore plate of the IGBT module for heat exchange, meanwhile, the cooling mechanism is matched for evaporative cooling of cooling water for heat exchange, and the automatic replacement mechanism can complete the replacement process of the filtering tank under the condition of continuous work without shutdown, so that stable operation of power equipment is guaranteed; meshes of the air inlet plate are cleaned through the fan cover anti-blocking mechanism, so that the meshes are prevented from being blocked; and in the cleaning process, the circulating cleaning mechanism is matched to automatically remove impurities such as fallen leaves in the cooling mechanism, and the labor cost of system management is reduced.

The invention provides a rotational flow multi-channel composite gas-liquid separation device and a separation method. The separation device comprises a shell assembly, and a gas inlet cavity, a transition cavity, a filter cavity and a discharge cavity which are separated and communicated in the shell assembly; an umbrella-shaped cover, an inner curved surface fin assembly, an outer curved surface fin assembly and a scroll plate are arranged in the air inlet cavity, and a liquid outlet is formed in the corresponding shell assembly below the scroll plate; the transition cavity is arranged above the side of the air inlet cavity, the filtering cavity is located on the side of the air inlet cavity, the upper end of the filtering cavity is communicated with the transition cavity, a micropore plate is arranged in the filtering cavity, a cavity, corresponding to the lower portion of the micropore plate, of the filtering cavity is communicated with the discharging cavity, an inlet of the exhaust port is communicated with the discharging cavity, and an outlet of the exhaust port is communicated with the outside. The device is reasonable in structural design, adopts an integrated design, adapts to the spatial layout of the electric vehicle, can effectively improve the separation efficiency, is reliable in liquid drainage, can effectively avoid reflux and gas leakage, and is high in working condition adaptability.

The utility model provides a dairy product detection workstation. The dairy product detection workstation comprises oscillation equipment, centrifugal equipment, reagent caching equipment, constant temperature equipment, a microplate reader and transfer equipment, the oscillation equipment is used for performing oscillation treatment on the container filled with the dairy products; the centrifugal equipment is used for performing centrifugal treatment on the centrifugal tube; the reagent caching equipment is used for storing a standard sample and a test reagent for testing, and the transfer equipment is used for mixing the test reagent with the standard sample and a dairy product sample respectively to obtain a first mixed solution and a second mixed solution; the constant-temperature equipment is used for heating and incubating the first mixed solution and the second mixed solution contained in the microwell plate; the microplate reader is used for detecting the incubated first mixed solution and second mixed solution to obtain the content of aflatoxin in the dairy product. And the transferring equipment can realize the transfer of the vessel and the solution among the equipment in the detection process. The dairy product detection workstation disclosed by the utility model can realize automation of aflatoxin detection, is high in detection efficiency and saves manpower.

The invention belongs to the technical field of petroleum and natural gas yield increase transformation, and provides a visual proppant migration experiment device used in a sand-carrying acid fracturing fracture, which comprises a plunger pump, a liquid supply tank, a liquid supply tank heater, a sand mixing tank, a gas supply bottle, a visual fracture system, a waste liquid tank, a computer data acquisition system and a high-speed camera. Two micropore plates of the visual crack system are arranged between a front cover plate and a rear cover plate in parallel at intervals, micropores are evenly distributed in the micropore plates, gas in the space between the front cover plate and the rear cover plate and the micropore plates can enter a crack space, and meanwhile the injection direction of the gas is changed into the transverse direction relative to the crack space from the vertical direction. The device can effectively simulate the influence of different sand-carrying fluid viscosities, gas volume proportions, sand ratios, particle sizes, tracing proppant proportions and temperatures on the migration of the proppant in the acid sand-carrying fracturing crack and the distribution form of the sand bank.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

Furniture such as desks and other items are provided with sound absorbing features consisting of micro-perforations extending through structures having vertical surfaces. The micro-perforations are formed using a LASER cutting tool or a punch and are either cylindrical or frustoconical. The micro-perforations are formed in drawer facings, doors, support structures, and free-standing objects and storage devices having vertical surfaces. The micro-perforations have diameters in the range of 0.20 to 0.70 mm. The micro-perforations have been found to increase sound absorption of a desk by as much as 4 Sabins within the frequency range of 100 to 5,000 Hz. Structural panels consisting of front and rear micro-perforated panels surrounding a porous central core may be employed in such furniture.

The utility model relates to the technical field of micro-pore plates, in particular to an improved shallow-pore pore plate which comprises a rotating structure used for improving the mounting efficiency and a pore plate body used for bearing the pore plate, a hole is formed in one side of the pore plate body, the rotating structure is mounted in the hole, and a base is fixedly connected to the bottom of the pore plate body. Through the arrangement of the rotating structure which comprises the fixing rod, the gasket, the circular ring, the square block, the clamping rod, the groove, the eccentric wheel, the supporting rod, the nut and other components, the rotating structure can drive the clamping rod to move in the groove by rotating the eccentric wheel through the mutual matching relation between the eccentric wheel component and the clamping rod component, and therefore the position of the clamping rod is adjusted; the pore plate body can be quickly mounted and dismounted through the rotating structure, the problems that a traditional pore plate is low in mounting efficiency and inconvenient to dismount are solved, and the mounting and dismounting efficiency of the pore plate is improved.

A fiber optic device and method for uniform illumination of biological samples distributed over one or more microcell arrays of a microwell plate can establish more reliable polymerase chain reaction (PCR) data sampling. In some embodiments, the apparatus includes a light engine that emits a light beam captured by a fiber optic bundle for delivering one or more light beams to each microchamber; each light beam thereby fluoresces the biological sample. The fiber optic cable of the fiber optic bundle comprises a distal output end held in place using a mechanical support for flexibly aligning the output beam of the distal output end to be arranged such that the center of the output beam is directed to different locations in the one or more microcell arrays, wherein each of adjacent ones of the output beams partially overlaps so that the light distribution is uniform.

1. The name of the design product: microplate reader. 2. The use of the design product: microplate reader is used for sample measurement analysis, and is used for reading various information contained in the microplate. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view. 5. Other changes in the state of the case need to be explained: each reference figure shows the light-emitting state of the progress bar panel when working in the microplate reader.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

An integrated magnetic microbead processing apparatus includes a microwell plate having magnetic microbeads in microwells. And the bottom of the micro-pore plate comprises cavities among the micro-pores. A heating / cooling plate supports the base of the microplate and has a plurality of pores. A vertically movable magnet support plate includes a plurality of magnetic pins, each of which may protrude through one of the plurality of apertures of the heating plate and into the cavity of the microwell plate. The height of the magnetic pin is adjustable. A vibrator is operably connected to the microwell plate and is configured to vibrate the microwell plate, the heating / cooling plate, the magnetic support plate.

A microplate stacker includes a housing having a microplate port at a first end of the housing and a confinement plane at a second end of the housing. A plurality of screws are disposed substantially orthogonal to the microwell plate and the confinement plane, and the plurality of screws are disposed between the microwell plate and the confinement plane, where each screw has a thread. A drive motor is provided in the housing for rotating each of the plurality of screws at substantially the same rotation rate and substantially the same rotation direction. A transmission is provided in the housing for coupling the drive motor to each of the plurality of screws.

The application discloses a multistage low-frequency sound absorption device, which comprises a microporous plate, an intermediate layer and a back plate. The intermediate layer is arranged between the microporous plate and the back plate to form a cavity. The intermediate layer is provided with at least one cavity module. Each cavity module comprises a plurality of parallelly arranged cavities with multiple stages. The microporous plate is provided with a plurality of through holes which are respectively communicated with the cavities. Cavities of the same stage have the same cross-sectional area, and cavities of different stages have different cross-sectional areas. The cross-sectional area difference of the cavities causes a scale effect. The cavities have different resonance frequencies under the scale effect, and each cavity obtains low-frequency broadband excellent sound absorption through coherent coupling effect under under-damping. The multistage low-frequency sound absorption device has the characteristics of low working frequency, large working bandwidth and continuous sound absorption peak. The cavities of the multistage low-frequency sound absorption device are expanded in the plane direction, and the height direction size is not increased, so that the height is small and the mass is light. The application also provides a parameter value method of the multistage low-frequency sound absorption device.

The invention relates to a solid fuel combustion characteristic test technology, and aims to provide a focusing single particle suspension variable atmosphere ignition combustion experiment device and method. A second microporous plate and a feed hopper are arranged at the top of a combustion chamber main body of the device, and a first microporous plate and a gas buffer cavity are arranged at the bottom; the two laser emitters are arranged in an included angle shape, and the energy of a single laser beam is lower than a fuel particle ignition threshold value; the two paths of laser intersect in the central area of the cavity to form a space energy focus, and the superposed energy of the two beams of laser can be absorbed at the position to reach an ignition threshold value; and light paths of diagnosis equipment in the optical acquisition module are intersected in a focus area. According to the invention, through a strategy of airflow disturbance and microwell plate blocking, particles completely move in airflow; the ignition behavior has spatial selectivity, the success rate is high and controllable, and the research is closer to the actual application scene. Through the design of single-particle fixed-point triggering, the system complexity and the control difficulty can be reduced, and the operation stability and the experiment repeatability can be improved.

The utility model discloses an integrated wastewater treatment device which comprises a treatment tank, a sewage pool is arranged in the treatment tank, a water inlet pipe is arranged at the position, corresponding to the upper end of the sewage pool, of the left side of the treatment tank, an ozone nano-bubble generating mechanism is arranged on the right side of the treatment tank, and the ozone nano-bubble generating mechanism comprises a mixing tank. The mixing tank is installed on the treatment tank, a mixing cavity is formed in the lower side of the interior of the mixing tank, the right end of the mixing cavity is connected with an air inlet pipe, a water inlet is formed in the lower end of the mixing cavity, a bubble generation chamber is arranged on the mixing cavity, and an air outlet is formed in the bubble generation chamber. According to the utility model, various organic wastewater which is difficult to degrade can be treated by utilizing the characteristic of high oxidation capacity of ozone to efficiently and indiscriminately oxidize strong oxidizing substances, and meanwhile, the microwell plate is utilized to primarily fill the ozone into bubbles in water to divide, so that the solubility of the ozone is increased, and the oxidation effect and the utilization rate of the ozone are enhanced.

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

The present invention discloses a drug sensitivity detection microwell plate, and relates to the technical field of microbiological detection, the drug sensitivity detection microwell plate comprises a bottom plate and a cover plate, the top of the bottom plate is integrally fixed with a culture plate body, and the height difference between the outer edge of the culture plate body and the bottom plate forms a step. In the using process of the culture plate, an operator only needs to execute the action of palm pressing, synchronous liquid supplementing operation on all the hole sites in the culture plate body can be synchronously achieved, the liquid supplementing efficiency is remarkably improved, the process of continuously executing the actions of turnover liquid supplementing, vertical liquid supplementing and return pressing is extremely simplified, and the operation efficiency is greatly improved. The method not only can effectively reduce the learning cost of a user and is beneficial to large-scale mass production and popularization, but also greatly shortens the time of cell liquid change compared with the traditional pipette liquid change, can effectively avoid long-time dehydration death of cells caused by overlong liquid change time, improves the quality of the cultured cells, and reduces the production cost of the cells. And the accuracy and success rate of the experiment are improved.