275 results about "Microwell Plate" patented technology

A temperature regulation system for samples in a microplate includes a housing defining an interior volume and first and second airflow ports defined by a first and second exterior surfaces of the housing. The system includes an electromagnetic mixing system that has a base plate defining a plurality of openings, and a plurality of electromagnets. Each electromagnet defines an axis that extends substantially vertically from the base plate. The base plate is disposed in the interior volume and each of the plurality of electromagnets extend toward the first airflow port. A fan is disposed in the interior volume substantially below the base plate. Activation of the fan draws air into the first or second airflow port, substantially parallel to each axis of the plurality of electromagnets, through the plurality of openings, and out of the other of the second and first airflow port.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention relates to the technical field of microplate readers, and discloses a multifunctional microplate reader which comprises a full-wavelength microplate reader body, a detection cavity used for detecting a sample is formed in the full-wavelength microplate reader body, and a microplate used for placing the sample is arranged in the detection cavity. The full-wavelength microplate reader body is rotationally connected with a cover plate for sealing the detection cavity through a rotating shaft, the full-wavelength microplate reader body is further provided with a control panel for controlling the device to be opened and closed, a sample taking and placing assembly is arranged in the detection cavity, and a cleaning assembly is arranged on the sample taking and placing assembly. And a wiping assembly is arranged on the microporous plate. The device effectively avoids the problem that the accuracy of a sample detection result is affected due to the fact that an existing device is difficult to clean the surface of the microporous plate, and is high in practicability.

The invention discloses an intelligent detection and analysis system for biological evaluation data of a medical instrument, and relates to the technical field of data processing, and the system comprises an image acquisition module which carries out the rapid imaging of the initial physical planking state of cells in a microwell plate; the image processing and feature quantification module is used for identifying the cell object and extracting a plurality of physical operation quality (POQ) feature parameters representing a population space distribution mode of the cell object; the quality evaluation and early warning module is used for comparing the POQ fingerprint with a preset quality baseline in real time and generating an early warning signal when the deviation exceeds a threshold value; and the data management and trend analysis module is used for continuously recording and analyzing POQ fingerprint historical data associated with the automatic pipetting equipment. According to the method, the physical operation quality is monitored at the experiment starting point, so that the accuracy, the reliability and the experiment efficiency of biological evaluation data of medical instruments are improved, the resource waste and the failure risk caused by initial inoculation errors are effectively reduced, and a way is provided for intelligent management of automatic equipment.

The invention discloses a design method of an array type silencer adaptive to a main ventilation air path between a stator iron core of a hydraulic generator and an air cooler and the array type silencer, and relates to the technical field of suppression or reduction of noise of the hydraulic generator. The method comprises the steps of S1, obtaining aerodynamic noise data and airflow parameters of a main ventilation air path, S2, determining a silencing thought and a micro-perforated plate array core structure according to broadband vortex, discrete rotation, vortex falling and surge noise, S3, determining the boundary dimension and preliminarily calculating the airflow parameters, S4, designing plate parameters in combination with a micro-perforated resonance formula, and S5, calculating the noise of the micro-perforated plate array. S5, performing simulation optimization and S6, accurately accounting airflow parameters, and locking a scheme meeting requirements; the silencer comprises a shell and a plurality of micro-perforated plates, and the multiple layers of micro-perforated plates are arranged in series. The invention has the advantages of no sound absorption material, low wind resistance (the air volume attenuation is less than or equal to 5%, and the pressure loss is less than or equal to 200Pa), the noise of the main wind path is reduced by 5-7dB (A), the service life is long, the operation and maintenance cost is low, and the requirements of the main wind path are met.

The invention discloses reflow soldering equipment and process for SMT patch production. The reflow soldering equipment comprises a furnace body base, a conveying unit, a hearth and a sealing top cover. A conveying unit is horizontally laid on the furnace body base, the hearth is installed on the upper end face of the furnace body base in a sealed mode, and the interior of the hearth is evenly divided into three independent working areas which are the preheating area, the backflow area and the cooling area in sequence from left to right. A visual inspection chamber is installed at the position, located at the feeding end of the hearth, of the upper portion of the furnace body base. A heating device is arranged in each working area of the hearth; in the heating device of the backflow area, each microplate is slidably provided with a vertically arranged hot spray pipe, and the hot spray pipes can perform hot air pulse direct blowing on large-size surface-mounted elements on a PCB, directly heat pin welding spots, realize micro-swing attachment of the elements in cooperation with surface tension of solder paste, and eliminate mechanical stress when the chip mounter is placed.

The invention provides a molecular modification design method for improving esterification activity of lipase. The method comprises the following steps: calculating a channel structure of lipase to be modified through CAVER ANALYST 2.0, determining a modification site through comprehensive measurement of other calculation tools, establishing a mutant library by adopting NNK degenerate codons for site-saturated mutation, and screening out excellent mutants with improved esterification performance by adopting a microwell plate screening method based on a copper soap complexing method. According to the candida antarctica lipase B mutant M5 disclosed by the invention, in various different types of esterification reactions, the catalytic efficiency of the mutant M5 is improved by 4.5 times or more than that of a wild type. The molecular modification design method disclosed by the invention can be used for improving the esterification performance of the lipase and has relatively wide esterification reaction application applicability, so that the lipase mutant can play an important role in industrial application in the fields of foods, medicines or biodiesel and the like.

The invention provides an ultrathin broadband sound absorption composite structure which comprises a plurality of silencing units, every two of the silencing units are in contact with each other on the same plane to form the three-dimensional ultrathin broadband sound absorption composite structure, each silencing unit comprises a shell, a through hole is formed in each shell, and the through holes are communicated with the silencing units. A thin film is arranged in the position, located in the center of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, a micro-pore plate is arranged in the position, located on the periphery of the three-dimensional ultra-thin broadband sound absorption composite structure, of the shell, and the micro-pore plate or the thin film or other resonance structures are arranged in the micro-pore plate or the thin film or other resonance structures in the micro-pore plate or the thin film or other resonance structures. The ultra-thin broadband sound absorption composite structure provided with the micropore plate and the thin film is small in size, the thickness of the ultra-thin broadband sound absorption composite structure is 1 / 23 of the sub-wavelength size, the ultra-thin broadband sound absorption composite structure has the ultra-thin characteristic, the sound absorption frequency band is wide, the noise reduction effect is good, and the ultra-thin broadband sound absorption composite structure can be suitable for sound absorption and noise reduction of various small places and spaces.

The invention relates to the technical field of power equipment cooling, and discloses a reactive power compensation device evaporative cooling system which comprises an SVG compensation cabinet, one side of the SVG compensation cabinet is provided with a cooling liquid discharge pipe and a cooling liquid access pipe, and the cooling liquid discharge pipe and the cooling liquid access pipe are both connected to a heat exchange mechanism. An automatic replacement mechanism is arranged in the heat exchange mechanism, and the heat exchange mechanism is connected to the cooling mechanism; according to the invention, the heat exchange mechanism is connected with the micro-pore plate of the IGBT module for heat exchange, meanwhile, the cooling mechanism is matched for evaporative cooling of cooling water for heat exchange, and the automatic replacement mechanism can complete the replacement process of the filtering tank under the condition of continuous work without shutdown, so that stable operation of power equipment is guaranteed; meshes of the air inlet plate are cleaned through the fan cover anti-blocking mechanism, so that the meshes are prevented from being blocked; and in the cleaning process, the circulating cleaning mechanism is matched to automatically remove impurities such as fallen leaves in the cooling mechanism, and the labor cost of system management is reduced.

The invention provides a rotational flow multi-channel composite gas-liquid separation device and a separation method. The separation device comprises a shell assembly, and a gas inlet cavity, a transition cavity, a filter cavity and a discharge cavity which are separated and communicated in the shell assembly; an umbrella-shaped cover, an inner curved surface fin assembly, an outer curved surface fin assembly and a scroll plate are arranged in the air inlet cavity, and a liquid outlet is formed in the corresponding shell assembly below the scroll plate; the transition cavity is arranged above the side of the air inlet cavity, the filtering cavity is located on the side of the air inlet cavity, the upper end of the filtering cavity is communicated with the transition cavity, a micropore plate is arranged in the filtering cavity, a cavity, corresponding to the lower portion of the micropore plate, of the filtering cavity is communicated with the discharging cavity, an inlet of the exhaust port is communicated with the discharging cavity, and an outlet of the exhaust port is communicated with the outside. The device is reasonable in structural design, adopts an integrated design, adapts to the spatial layout of the electric vehicle, can effectively improve the separation efficiency, is reliable in liquid drainage, can effectively avoid reflux and gas leakage, and is high in working condition adaptability.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

Furniture such as desks and other items are provided with sound absorbing features consisting of micro-perforations extending through structures having vertical surfaces. The micro-perforations are formed using a LASER cutting tool or a punch and are either cylindrical or frustoconical. The micro-perforations are formed in drawer facings, doors, support structures, and free-standing objects and storage devices having vertical surfaces. The micro-perforations have diameters in the range of 0.20 to 0.70 mm. The micro-perforations have been found to increase sound absorption of a desk by as much as 4 Sabins within the frequency range of 100 to 5,000 Hz. Structural panels consisting of front and rear micro-perforated panels surrounding a porous central core may be employed in such furniture.

The utility model relates to the technical field of micro-pore plates, in particular to an improved shallow-pore pore plate which comprises a rotating structure used for improving the mounting efficiency and a pore plate body used for bearing the pore plate, a hole is formed in one side of the pore plate body, the rotating structure is mounted in the hole, and a base is fixedly connected to the bottom of the pore plate body. Through the arrangement of the rotating structure which comprises the fixing rod, the gasket, the circular ring, the square block, the clamping rod, the groove, the eccentric wheel, the supporting rod, the nut and other components, the rotating structure can drive the clamping rod to move in the groove by rotating the eccentric wheel through the mutual matching relation between the eccentric wheel component and the clamping rod component, and therefore the position of the clamping rod is adjusted; the pore plate body can be quickly mounted and dismounted through the rotating structure, the problems that a traditional pore plate is low in mounting efficiency and inconvenient to dismount are solved, and the mounting and dismounting efficiency of the pore plate is improved.

A fiber optic device and method for uniform illumination of biological samples distributed over one or more microcell arrays of a microwell plate can establish more reliable polymerase chain reaction (PCR) data sampling. In some embodiments, the apparatus includes a light engine that emits a light beam captured by a fiber optic bundle for delivering one or more light beams to each microchamber; each light beam thereby fluoresces the biological sample. The fiber optic cable of the fiber optic bundle comprises a distal output end held in place using a mechanical support for flexibly aligning the output beam of the distal output end to be arranged such that the center of the output beam is directed to different locations in the one or more microcell arrays, wherein each of adjacent ones of the output beams partially overlaps so that the light distribution is uniform.

1. The name of the design product: microplate reader. 2. The use of the design product: microplate reader is used for sample measurement analysis, and is used for reading various information contained in the microplate. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view. 5. Other changes in the state of the case need to be explained: each reference figure shows the light-emitting state of the progress bar panel when working in the microplate reader.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

A microplate stacker includes a housing having a microplate port at a first end of the housing and a confinement plane at a second end of the housing. A plurality of screws are disposed substantially orthogonal to the microwell plate and the confinement plane, and the plurality of screws are disposed between the microwell plate and the confinement plane, where each screw has a thread. A drive motor is provided in the housing for rotating each of the plurality of screws at substantially the same rotation rate and substantially the same rotation direction. A transmission is provided in the housing for coupling the drive motor to each of the plurality of screws.

The application discloses a multistage low-frequency sound absorption device, which comprises a microporous plate, an intermediate layer and a back plate. The intermediate layer is arranged between the microporous plate and the back plate to form a cavity. The intermediate layer is provided with at least one cavity module. Each cavity module comprises a plurality of parallelly arranged cavities with multiple stages. The microporous plate is provided with a plurality of through holes which are respectively communicated with the cavities. Cavities of the same stage have the same cross-sectional area, and cavities of different stages have different cross-sectional areas. The cross-sectional area difference of the cavities causes a scale effect. The cavities have different resonance frequencies under the scale effect, and each cavity obtains low-frequency broadband excellent sound absorption through coherent coupling effect under under-damping. The multistage low-frequency sound absorption device has the characteristics of low working frequency, large working bandwidth and continuous sound absorption peak. The cavities of the multistage low-frequency sound absorption device are expanded in the plane direction, and the height direction size is not increased, so that the height is small and the mass is light. The application also provides a parameter value method of the multistage low-frequency sound absorption device.

The invention relates to a solid fuel combustion characteristic test technology, and aims to provide a focusing single particle suspension variable atmosphere ignition combustion experiment device and method. A second microporous plate and a feed hopper are arranged at the top of a combustion chamber main body of the device, and a first microporous plate and a gas buffer cavity are arranged at the bottom; the two laser emitters are arranged in an included angle shape, and the energy of a single laser beam is lower than a fuel particle ignition threshold value; the two paths of laser intersect in the central area of the cavity to form a space energy focus, and the superposed energy of the two beams of laser can be absorbed at the position to reach an ignition threshold value; and light paths of diagnosis equipment in the optical acquisition module are intersected in a focus area. According to the invention, through a strategy of airflow disturbance and microwell plate blocking, particles completely move in airflow; the ignition behavior has spatial selectivity, the success rate is high and controllable, and the research is closer to the actual application scene. Through the design of single-particle fixed-point triggering, the system complexity and the control difficulty can be reduced, and the operation stability and the experiment repeatability can be improved.

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

An organoid probing system includes a probe head having a probe, a first stage along which the probe head is movable in an X direction, and a second stage configured to move a microwell plate in a Y direction. The microwell plate has a microwell containing an organoid. The probe head is movable in a Z direction to insert the probe into the microwell or retract the probe away from the organoid in order to measure a property of the organoid.

Provided herein is a stimulation and measurement device. The stimulation and measurement device includes a microwell plate having a top side, a bottom side, and at least one well extending from the top side towards the bottom side; a first assembly removably positioned on the top side of the microwell plate; a second assembly removably positioned on the bottom side of the microwell plate; and a microcontroller. The first assembly includes a first printed circuit board having at least one pair of stimulating light emitting diodes (LEDs) positioned thereon to align with one of the wells. The second assembly includes a second printed circuit board having at least one photodiode positioned thereon to align with one of the pairs of stimulating LEDs, and at least one excitation LED paired with each of the photodiodes. The microcontroller is configured to control the stimulating LED(s), the photodiode(s), and the excitation LED(s).

The industrial design sought to be registered is primarily a well plate for laboratory use, covering the front, back, right and left sides and featuring a peripheral ridge structure interrupted by the four corners and the back. 1.1) Front view; 1.2) Back view; 1.3) Right side view; 1.4) Left side view; 1.5) Top view; 1.6) Bottom view; 1.7) Perspective view

An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.

The invention discloses an imaging analysis method adopting a dome microscope light source. Relates to the technical field of microscopes. The method comprises the following steps: inoculating cells into micropores of a cell culture plate; placing the cell culture plate below a dome microscope light source, focusing, moving the cell culture plate after the focusing is clear, and enabling the field of view of the dome microscope to be at the edge of the micropore of the cell culture plate; turning on a middle light source in the light source inner ring of the dome microscope, adjusting the brightness of the middle light source and the dome light source, and obtaining a clear cell culture plate edge image by automatically adjusting the brightness and automatically analyzing the collected image; and splicing a plurality of images to obtain a complete and clear image of the whole micropore. According to the invention, the problem of imaging shadow caused by the concave liquid surface effect in the traditional inverted microscopic imaging experiment is effectively solved. The light of the light source can better penetrate through the concave liquid surface and uniformly irradiate each part of the microwell plate, so that the imaging of the edge of the microwell does not have a shadow any more, and the quality and definition of the imaging are improved.

The utility model discloses a microwell plate of a magnetic bead kit, and relates to the field of microwell plates, the microwell plate comprises a plate body and a plurality of mounting mechanisms mounted on the plate body, the outer wall of the top of the plate body is provided with a plurality of circular openings distributed at equal intervals, the mounting mechanisms are mounted in the circular openings, and each mounting mechanism comprises a mounting sleeve fixedly connected in the corresponding circular opening; the section of the mounting sleeve is of a T-shaped structure, two symmetrically-arranged locking assemblies are mounted on the outer wall of the mounting sleeve, and two symmetrically-arranged clamping assemblies are mounted on the outer wall of the bottom of the mounting sleeve. According to the microplate, the mounting mechanism is arranged on the microplate body, and the mounting sleeve and the locking assembly in the mounting mechanism are matched, so that test tubes of different specifications can be conveniently fixed in the mounting sleeve, and the problem that only test tubes of a single specification can be mounted on an existing microplate is solved.

The utility model relates to a conference room fabric sound-absorbing microwell plate mounting structure, and relates to the field of fabric sound-absorbing microwell plate mounting. The device comprises a fixing frame, a sound absorption plate is arranged on the front face of the fixing frame, a clamping groove is formed in the front face of the fixing frame, a sliding block is arranged on the back face of the sound absorption plate, and two symmetrical sliding grooves are formed in the top of the sliding block. According to the device, the damaged acoustic board is dismounted and pushed, so that the sliding blocks enter the inner cavities of the clamping grooves, the clamping blocks are extruded to enter the inner cavities of the sliding grooves at the moment, the springs contract, and when the sliding blocks enter the inner cavities of the clamping grooves, the springs push the two clamping blocks to deviate from each other, the sliding blocks are limited to the front face of the fixing frame, and therefore the fixing effect is achieved. The sound-absorbing plate is fixed on the wall surface to complete the replacement of the sound-absorbing plate, so that a user does not need to dismantle all the sound-absorbing micropore plates nearby the sound-absorbing micropore plates to replace and install the sound-absorbing micropore plates, and the replacement efficiency of the sound-absorbing micropore plates is improved.

The invention discloses a digoxin drug concentration detection method, which comprises: S1, determining a to-be-detected drug, and carrying out working curve preparation on a detection object, the to-be-detected drug being digoxin; s2, taking out the to-be-detected sample, the calibration product, the quality control product and the internal standard extraction liquid, placing at room temperature, and uniformly mixing; s3, respectively taking a to-be-detected sample, a calibration product, a quality control product and a blank sample, putting into an EP tube, adding the internal standard intermediate solution, adding methanol, uniformly mixing by vortex, and centrifuging; s4, putting the supernate into a 96-well plate, adding ultrapure water, and putting the 96-well plate on a microwell plate oscillator for shaking; and S5, centrifuging in a multi-tube rack automatic balancing centrifuge, detecting and verifying by using liquid chromatogram-tandem mass spectrum, and collecting a mass spectrum signal. According to the present invention, the high performance liquid chromatography separation and the mass spectrometry are combined, such that the complex background interference in the blood sample is overcome, the cost performance is improved, and the high sensitivity, the high accuracy and the convenient detection can be achieved for the digoxin sample with the low in-vivo concentration.

The invention provides a device and a method for measuring a high-speed bottom flow velocity field based on micropore injection. The device comprises a power source, a particle storage container, a model supporting piece and a model, the power source is communicated with the particle storage container and is used for providing power for injection of the tracer particles; one end of the model supporting piece extends into the model and is used for supporting the model, the other end of the model supporting piece is connected with the particle storage container, a ventilation pipeline is arranged in the model supporting piece, and tracer particles can be injected into the model along the ventilation pipeline under the action of power provided by the power source; a first cavity and a second cavity which are sequentially communicated with the ventilation pipeline are formed in the model, a first microwell plate is installed between the first cavity and the second cavity, and a second microwell plate is installed at the end, away from the first cavity, of the second cavity. According to the device and the method, through development of a bottom flowing space device, supplement of bottom tracer substances is achieved, and the problem that high-speed bottom flowing tracer substances are difficult to enter is solved.

This utility model relates to the field of screening equipment technology, and more particularly to a screening device for covalent inhibitor reactions. It includes a screening device body, on the inner wall of which a microwell plate is installed. Four foot pads are installed on one side of the screening device body. An adjustment mechanism is provided at one end of the screening device body. The adjustment mechanism includes two fixing plates, each fixedly connected to one end of the screening device body. A motor is fixedly connected to one side of each fixing plate, and a gear is fixedly connected to the output end of the motor. The gear teeth mesh with a rack. This utility model provides a covalent inhibitor reaction screening device that allows the microwell plate to be placed in the screening device by means of the adjustment mechanism, replacing manual labor. The adjustment mechanism improves the stability of microwell plate placement, reduces manpower consumption, increases laboratory efficiency, and enhances experimental accuracy.