The application provides a method for nondestructive detection of the contents of EPA and DHA in
scallop adductor
muscle based on Raman spectrum, which comprises the following steps: firstly, a proper amount of
gill filament tissue is taken from a living
scallop by a nondestructive method, and then a frozen section is prepared; secondly, the section is subjected to
Raman laser scanning to obtain
original data; and finally, after pretreatment such as normalization,
noise reduction and baseline correction, a characteristic
wavelength is selected, and a model for predicting the content of
unsaturated fatty acid in the adductor
muscle by using the
gill filament Raman data across tissues is established in combination with the lipid group data of the adductor
muscle. Compared with the existing method for detecting the content of
unsaturated fatty acid in scallops, the determination method of the application can realize the rapid, accurate and simple detection of the content of
unsaturated fatty acid in the main edible part, i.e., the striated muscle of scallops, under the condition of ensuring the survival of scallops, and greatly reduces the detection cost, thereby providing an efficient and simple technical means for the breeding of
shellfish quality traits, and being beneficial to the popularization and promotion in the comprehensive evaluation and genetic improvement of aquatic
germplasm.