154 results about "Stimulated raman" patented technology

The invention discloses a digital imaging method of an ear-nose-throat examination endoscope, and relates to the technical field of medical treatment, and the method comprises the following steps: synchronously collecting a white light image, a photoacoustic signal, a stimulated Raman spectrum, pressure sensor data and a physiological signal of a target orifice through a multi-physical field probe; constructing a four-dimensional tensor bound with the anatomical features; based on a vocal cord vibration fundamental frequency harmonic characteristic optimization graph convolutional network, dynamically constructing an adjacent matrix and coupling cross-modal characteristics to generate a submucosal lesion enhanced image; a deformable convolutional network constrained by vocal cord biomechanics is adopted, and the shape of a convolution kernel is dynamically adjusted according to the relation between real-time strain and elastic modulus, so that motion artifacts caused by swallowing actions are inhibited; generating a curvature-driven asymmetric convolution kernel based on ear canal spiral geometry, and executing super-resolution reconstruction in combination with confrontation training of fractal constraint; and fusing the white light image gradient and the pressure gradient field, and outputting a three-dimensional lesion contour consistent with the anatomical structure.

The invention discloses a tunable excited Raman spectrum microimaging method and device, and belongs to the field of spectrum detection and optical imaging. The device comprises a broadband adjustable wavelength light source, a high-resolution wavelength scanning module, a stimulated Raman signal excitation module and a stimulated Raman signal detection module. The stimulated Raman signal excitation and imaging module is used for focusing the two beams of narrow-band femtosecond pulse light which are overlapped in time and space on a sample and exciting a stimulated Raman signal, and meanwhile, moving the sample to realize spot scanning microscopic imaging; and the stimulated Raman signal detection module is used for converting the stimulated Raman scattering signal into an electric signal, demodulating the electric signal by using a lock-in amplifier, and recording spectral information by using a data acquisition card. According to the invention, the broadband tunable characteristic of the optical parametric amplifier is utilized to tune the pump light to the wavelength region of the sample absorption peak length so as to realize electron pre-resonance stimulated Raman; and a balance detection technology is combined at a detection end, so that high-sensitivity, high-resolution and high-signal-to-noise-ratio detection of stimulated Raman signals is realized.

The invention relates to the technical field of spectral imaging detection, in particular to a spontaneous-stimulated Raman co-localization single-cell substructure atlas analysis system and a spontaneous-stimulated Raman co-localization single-cell substructure atlas analysis method. The system comprises a femtosecond pulse excitation module used for outputting two beams of broadband femtosecond pulse laser with different frequencies and continuously adjustable optical power; the Stokes light modulation module is used for modulating the Stokes light and carrying out space beam combination on the Stokes light and the pump light; the pump light modulation module is used for modulating pump light; the spontaneous Raman laser continuous adjusting module is used for outputting spontaneous Raman laser; the stimulated Raman exciting light co-positioning module is used for combining the combined laser and the spontaneous Raman laser again, focusing the combined laser and the spontaneous Raman laser on a sample, exciting a stimulated Raman scattering signal, converting the stimulated Raman scattering signal into a digital signal and carrying out data acquisition; and the spectrum detection module is used for collecting the spontaneous Raman scattering light which is reflected and returned along the original light path, and collecting a spectrum signal to form a Raman spectrum. The method has the advantage of improving the accuracy of single-cell heterogeneity structure and cancer cell type discrimination.

The invention belongs to the technical field of atomic inertial measurement, and discloses a continuous measurement atomic interferometer control method and system. The method comprises the steps that atoms are sequentially subjected to N times of stimulated Raman transition, the time difference between the stimulated Raman transition is T, atom interference is achieved, in the same vacuum cavity, a plurality of atomic groups are sequentially subjected to atom interference, when the Kth atomic group is subjected to the nth time of stimulated Raman transition, the (K + 1) th atomic group and the Kth atomic group share Raman laser, and the Kth atomic group and the (K + 1) th atomic group share Raman laser. And the first stimulated Raman transition of the (K + 1) th atomic group is synchronously carried out, so that the atomic interference of the atomic group in the vacuum cavity at each moment can be ensured, the output result of the interferometer covers the working time of the whole interferometer, the continuous measurement of the atomic interferometer is realized, and n is less than or equal to N.

The invention relates to a device and a method of a high-sensitivity suspension single-droplet sensor for monitoring PM2.5 pollution, belongs to the technical field of aerosol microdroplet spectrum trace analysis, and solves the problem that ppb-magnitude gas cannot be monitored in the prior art. The method comprises the following steps: pumping aerosol into a sample cell of a droplet suspension system in a single droplet form; introducing required reaction gas into the sample pool through an atmosphere control system; scattered light signals of the single liquid drops are respectively input into the dynamic signal processing system and the microdroplet imaging system; acquiring the characteristic peak displacement change of the metal ion sensing microdroplet; establishing a gas particle distribution Mie scattering inversion model based on micro-droplet dynamic response; inverting the radius of the liquid drop according to the stimulated Raman scattering / stimulated elastic scattering signal; according to the quantitative relationship of the reaction, calculating the generation of sulfate by the droplet suspension system (sensor); and judging which gas in the actual atmosphere is a main oxidation pathway for oxidizing sulfur dioxide to generate sulfate pollutants according to a calculation result.

The invention belongs to the field of image processing, and particularly discloses a stimulated Raman microscopic image reconstruction method and system based on a diffusion model, and the method comprises the steps: obtaining a to-be-reconstructed LR image; inputting the LR image into a trained diffusion model to obtain a high-frequency image; performing denoising processing on the LR image to obtain a denoised image; adding the de-noised image and the high-frequency image to obtain a reconstructed image; wherein the diffusion model is obtained by constructing a data set according to PSF microscopic images of a stimulated Raman scattering imaging system and carrying out noise prediction training on the data set. According to the invention, the reconstruction quality of the image can be improved.

The utility model discloses a special glass slide for a stimulated Raman scattering imaging microscope, which comprises a glass slide, a cover glass and a buckle, the glass slide is in limit connection with the cover glass through the buckle, a sample groove is arranged on the glass slide, the cover glass covers the sample groove, so that a cavity is formed between the glass slide and the cover glass, and the sample groove is arranged in the cavity. And the side surface of the glass slide is provided with at least two small holes which are directly communicated with the inner cavity of the sample groove. According to the utility model, water evaporation can be prevented, bubbles can be avoided, water can be replenished without uncovering the cover glass, and laser is ensured to be focused on the same plane of a sample during imaging.

The invention belongs to the field of plant insect-resistant characteristic analysis, and particularly discloses a real-time in-situ detection method and system for the insect-resistant level of a plant, and the method specifically comprises the following steps: after a to-be-detected plant is irradiated by ultraviolet light of different doses, a hyperspectral stimulated Raman scattering imaging subsystem is adopted to carry out stomata imaging and spectral signal acquisition on leaves of the to-be-detected plant; performing qualitative analysis on different metabolites in the leaves by adopting spectral signals of different wavebands, and performing quantitative detection on the metabolites in combination with characteristic peak areas and peak intensity characteristics; acquiring pore structure change based on pore imaging; and calculating the correlation between the pore structure and metabolite change of the plant caused by different irradiation and the insect resistance, thereby realizing in-situ detection of the insect resistance of the plant. According to the method provided by the invention, the complex process of plant disease and insect pest resistance can be comprehensively analyzed, and the non-invasiveness, high specificity and qualitative and quantitative capability can provide important theoretical basis and practical guidance for developing a green disease and insect pest control technology.

The invention belongs to the technical field of optical microscopic imaging, and particularly relates to a coherent Raman microscopic imaging method based on a planar parallel microcavity. According to the invention, a plane parallel micro-cavity structure, namely a Fabry-Perot resonant cavity, is used for traditional coherent Raman microscopic imaging for the first time, micro-cavity structures with different reflectivity coating films are designed, and coherent anti-Stokes Raman scattering and stimulated Raman scattering processes are respectively regulated and controlled. And coupling the spatial structure information of the sample with a Raman signal to generate a new spectrum and image, and respectively realizing cavity-enhanced coherent anti-Stokes Raman scattering imaging and cavity-enhanced stimulated Raman scattering imaging. Compared with traditional coherent Raman microscopic imaging, the signal intensity is improved, spatial structure information and chemical information of a sample are obtained at the same time, so that more-dimensional information of the sample is provided, and compared with traditional biological microcavity sensing imaging, rapid unmarked imaging can be achieved; the method has wide application prospects in the fields of biomedical detection and the like.

Multiplex CARS microscopy device for analysing a sample (Ech) comprising: -a laser source (LS) suitable for emitting a primary beam (FP) having a first wavelength λ1, in the form of pulses (IL1) with a power called the primary power; -an optical fibre (F) supporting fewer than ten modes, said pulses propagating through the optical fibre (F) in anomalous dispersion regime so as to generate, from the primary beam, an output beam (FSC) containing a plurality of second wavelengths forming a supercontinuum (SC), and said first wavelength λ1, the second wavelengths being generated by non-linear conversion of the first wavelength λ; -an optical system (MO) suitable for focusing the output beam onto said sample, so as to generate an anti-Stokes beam (STK) via stimulated Raman scattering induced by at least one of the second wavelengths and the first wavelength λ1 present in the output beam; -a photodetector (Det) suitable for detecting the anti-Stokes beam.

The present disclosure provides computer-implemented methods, systems, and devices for non-invasively measuring a level of an analyte in a user using Stimulated Raman Scattering. A device can include a Raman pump source that, when engaged, emits pump light toward a skin surface of the user at a pump wavelength. The device can further include a Stokes source that, when engaged, emits Stokes light toward the skin surface at one or more Stokes wavelengths. The device can further include a photodetector that measures light that emanates from the skin surface. The device can further include control circuitry configured to pulse modulate the Raman pump source and the Stokes source in accordance with at least one duty cycle. The device can further include a processor that processes the measured light to provide an estimated analyte level of the analyte in the user.

A stimulated Raman photothermal (SRP) microscope for imaging a sample. A first optical source without an optical resonator emits a pump beam. A second optical source emits an intensity-modulated Stokes beam. The Stokes beam is combined with the pump beam to form a combined beam. The combined beam is directed to the sample to induce a thermal effect caused by the stimulated Raman process. A third optical source emits a probe beam, the probe beam is directed to the sample. An optical detector detects modulation of the probe beam after modulation by the sample to measure an SRP signal. Because noise in the pump and Stokes beam do not significantly effect the measurements from the probe beam, these beams can use a high-powered optical parametric amplifier (OPA) source for improved sensitivity and imaging speed compared to SRP microscopes using an optical parametric oscillator (OPO) source.

The invention provides a C + L + S wave band multi-device configuration optimization method and device, and the method comprises the steps: calculating the approximate optimal transmission power of an optical fiber entrance part through employing a local optimal approximate global optimal algorithm; taking the approximate optimal transmitting power as the receiving power of each span section, and recalculating the transmitting signal power at the starting position of the span section by reversely solving a stimulated Raman scattering effect power evolution equation; the gain and gain slope of the optical fiber amplifier are set, so that the signal power which begins to be input in each span segment approaches the transmitting signal power; constructing a loss function based on the generalized signal-to-noise ratio of each wave band; and the gain and the gain slope set after inversion of the stimulated Raman scattering effect are used as initial values, and the gain and the gain slope of the optical fiber amplifier and the channel loss value of the wavelength selection switch are iteratively optimized by using a gradient rising algorithm and taking the loss function improvement as a target. According to the method provided by the invention, only a small amount of iteration is needed, the operation speed is high, and multi-device second-level configuration optimization of the broadband optical transmission system can be realized.

The invention provides a Raman spectrum calibration method and system based on transient stimulated Raman scattering. The Raman spectrum calibration method comprises the following steps: acquiring stimulated Raman signals in a time domain in a transient stimulated Raman scattering mode; the time delay of each sampling point of the time domain Raman signal is tracked in real time through the reference laser interference fringes, and Raman displacement after Fourier transform is determined; detecting an exciting light spectrum obtained by sampling through a photoelectric detector, and calculating excitation efficiency at different Raman displacement positions to obtain an excitation efficiency curve; the intensity of Raman spectrums at different Raman displacement positions is calibrated through the excitation efficiency curve, the self-calibrated Raman spectrums are obtained, the self-calibration transient stimulated Raman scattering technology inherits the advantages of high time resolution and high spectral resolution of the transient stimulated Raman scattering technology, the self-calibration transient stimulated Raman scattering technology is combined with a self-calibration mechanism, and the self-calibration effect of the self-calibration transient stimulated Raman scattering technology is improved. The influence of excitation light fluctuation is eliminated, the Raman spectrum measurement accuracy and repeatability are remarkably improved, and the quantitative analysis capability is comparable to that of spontaneous Raman spectrum.

A stimulated Raman photothermal (SRP) microscope for imaging a sample. A first optical source without an optical resonator emits a pump beam. A second optical source emits an intensity-modulated Stokes beam. The Stokes beam is combined with the pump beam to form a combined beam. The combined beam is directed to the sample to induce a thermal effect caused by the stimulated Raman process. A third optical source emits a probe beam, the probe beam is directed to the sample. An optical detector detects modulation of the probe beam after modulation by the sample to measure an SRP signal. Because noise in the pump and Stokes beam do not significantly effect the measurements from the probe beam, these beams can use a high-powered optical parametric amplifier (OPA) source for improved sensitivity and imaging speed compared to SRP microscopes using an optical parametric oscillator (OPO) source.

An Optical Time-Domain Reflectometer (OTDR) trace correction method, an electronic device, and a storage medium are disclosed. The method may include: establishing a parameter decoupling equation according to a fiber loss coefficient and a Stimulated Raman Scattering (SRS) transfer coefficient in response to ignoring an SRS transfer amount amplified or absorbed by an OTDR wavelength in an Optical Transport Network (OTN) service; disabling a service wavelength of an OTN of an optical fiber transmission system, and acquiring, according to the parameter decoupling equation, a first OTDR trace of an OTDR simulation wavelength in the optical fiber transmission system when the service wavelength of the OTN is in a disabled state; determining a fiber loss coefficient according to the first OTDR trace.

The present application belongs to the field of optical microscopic imaging technology, and particularly relates to a coherent Raman microscopic imaging method based on a plane-parallel microcavity. The present application first applies a plane-parallel microcavity structure, i.e. a Fabry-Perot resonant cavity, to a traditional coherent Raman microscopic imaging, designs a microcavity structure with different reflectivity coating, and respectively controls coherent anti-Stokes Raman scattering and stimulated Raman scattering processes, so as to couple spatial structure information of a sample and Raman signals to generate new spectra and images, and respectively realize cavity-enhanced coherent anti-Stokes Raman scattering imaging and cavity-enhanced stimulated Raman scattering imaging. Compared with the traditional coherent Raman microscopic imaging, the present application improves signal intensity, and simultaneously acquires spatial structure information and chemical information of a sample, so as to provide more dimensional information of the sample, and compared with the traditional biological microcavity sensing imaging, can realize fast and label-free imaging, and has wide application prospects in the fields of biomedical detection and the like.

The invention relates to the technical field of ultrafast laser, in particular to an ultraviolet laser damage inhibition method and device for a fused quartz optical element, and the method comprises the steps: obtaining oxy-18 modified DKDP crystals with oxy-18 isotope abundance higher than natural abundance; preparing the oxy-18 modified DKDP crystal into a wave plate matched with a preset ultraviolet laser wavelength; in an ultraviolet laser light path of a laser inertial confinement fusion device, a wave plate is arranged between a linear polarization state output end of ultraviolet laser and a fused quartz optical element; and enabling ultraviolet laser to sequentially pass through the linear polarization state output end and the wave plate to form circularly polarized light, and irradiating the circularly polarized light to the fused quartz optical element. The invention aims to realize the small-scale self-focusing effect of actively inhibiting fused quartz and solve the technical problem that a traditional material cannot be used as a large-aperture wave plate due to over-high transverse stimulated Raman scattering.

The invention relates to the technical field of optical fiber lasers, and proposes an ultra-wideband tunable optical fiber communication light source based on gas stimulated Raman scattering, comprising a 1μm band seed source, an electro-optic modulator, a signal generator, an optical fiber amplifier, a solid-hollow compact coupling device, an optical fiber end cap, a hollow-core optical fiber, a micro gas cavity, a lens, a dichroic mirror, and a methane gas 1μm band tunable pump source. The tuning range covers at least 1024nm-1126nm. Based on the stimulated Raman scattering effect of methane gas, it can be converted into a communication band laser covering the entire S+C+L+U band. Thanks to the Raman characteristics of methane molecules, the output laser has a corresponding narrow line width single spectral line under single wavelength pumping, without accompanying ASE noise amplification, etc., and does not need to be selected through secondary filtering. At the same time, the use of the hollow-core optical fiber ensures that the output laser is only single-mode, and high beam quality can be guaranteed without other operations.

The invention relates to the technical field of microscopic imaging, and particularly provides a stimulated Raman scattering microscopic imaging system capable of simultaneously scanning double Raman frequency shift spectrums, which comprises a laser light source driving and modulating unit and a signal detecting and data processing unit, the laser light source driving and modulating unit comprises a dual-channel laser synchronous excitation module, a light beam polarization regulation and power distribution module, a pulse broadening and spectrum focusing module, a dual-channel dual-phase modulation module, a common-path different-time dual-frequency difference module and a laser scanning and telecentric focusing module; and the signal detection and data processing unit comprises a dual-channel orthogonal demodulation module and a scanning driving and signal synchronous acquisition module. Compared with the prior art, the dual-Raman frequency shift spectrum scanning stimulated Raman microimaging device has the advantages that dual-Raman frequency shift spectrum scanning stimulated Raman microimaging with higher acquisition speed, high resolution and wide spectrum coverage range is realized, and the problem of long time consumption for acquiring the complete spectrum of the to-be-tested sample by the traditional single-frequency resonance spectrum scanning stimulated Raman microimaging is solved.

The invention provides a wide-band fast wavelength tuning light source device and method, and belongs to the field of laser technologies and precise spectrums.The wide-band fast wavelength tuning light source device comprises a first laser source and a second laser source, the first laser source generates first coherent light, the first coherent light is coupled into an annular resonant cavity, and the second laser source generates second coherent light; the first laser source generates first coherent light, optical parametric conversion is achieved at the first nonlinear crystal, idle light and signal light are output, the second laser source generates second coherent light, and the second coherent light is coupled into the annular resonant cavity from the other port. First coherent light and second coherent light which are left after optical parametric conversion at the first nonlinear crystal are coaxially injected into a second nonlinear medium, so that stimulated Raman intensity amplification and phase change of the first coherent light are realized; the intensity of the second coherent light injected into the second nonlinear medium is regulated and controlled, so that the three-wave phase matching condition in the resonant cavity can be quickly regulated and controlled, rapid and broadband wavelength tuning of signal light and idle light is realized, and the technical blank of broadband rapid wavelength tuning is filled.

A pulse generation unit generates a driving voltage signal including a pulse signal. A laser oscillation module oscillates a laser beam by carrying out, based on the driving voltage signal, a pulse oscillating operation. When the power command signal has a voltage command value corresponding to a laser power greater than a predetermined laser power during a high period, the pulse generation unit modulates, in a pulsed manner, a voltage value of the high period of the driving voltage signal so as to alternately repeat, for a preset period of time from a rising time of the high period of the driving voltage signal, a high state in which the voltage value is maintained and a low state in which the voltage value is lowered by a predetermined voltage value without being lowered to a voltage value of a low period of the driving voltage signal.

The invention discloses a handheld rapid three-dimensional fundus blood supply imaging system and method.The system comprises an upper computer, a laser excitation module, a probe, a data collection module and a space positioning module, the laser excitation module, the probe, the data collection module and the space positioning module are connected with the upper computer, and the laser excitation module conducts wavelength conversion on input pump laser through the stimulated Raman scattering principle to generate excitation light; the exciting light is then transmitted to the probe for high-speed scanning, the probe receives real-time pose feedback of the space positioning module at the same time so as to eliminate the influence of operation jitter, the data acquisition module pre-processes a photoacoustic signal acquired by the probe and then transmits the photoacoustic signal to the upper computer, and a distribution diagram is generated through an image reconstruction algorithm and analyzed. According to the invention, four clinical troubles of poor molecular specificity, depth-resolution imbalance, low clinical adaptability and deficiency of quantitative standards in the existing imaging technology are fundamentally solved, and a universal tool is provided for early screening of eye ground ischemic diseases.

This invention provides a device and method for generating simultaneously emitted three wavelengths of laser light with equal spacing, relating to the field of laser technology. The device includes a pump source, a half-wave plate, an optical isolator, a focusing lens, an input mirror, a Raman crystal, an output mirror, a nonlinear crystal, and a dichroic mirror arranged sequentially along the optical path. The pump light, after its polarization direction is adjusted by the half-wave plate and the optical isolator, is focused onto the Raman crystal. Stimulated Raman scattering generates first and second Stokes beams, which are then converted to sum-frequency light by the sum-frequency effect of the nonlinear crystal. Finally, the dichroic mirror outputs three equally spaced wavelengths of laser light. This invention separates the Raman conversion and sum-frequency processes through an external cavity structure, achieving for the first time the simultaneous emission of three equally spaced wavelengths of laser light, offering advantages such as high output energy, equal wavelength spacing, and simultaneous emission of three wavelengths.

The invention discloses a photoacoustic stimulated Raman gas detection system and method based on hollow-core optical fiber self-seed injection enhancement, and belongs to the technical field of laser spectrum trace gas detection, the system comprises a light source and beam splitting module, a hollow-core optical fiber seed generation module, a light delay module and a short optical path power amplification module; the main laser beam is split into a first light beam and strong pump light after entering the beam splitter; in the hollow-core optical fiber seed generation module, a first light beam passes through a fifth reflecting mirror, a hollow-core optical fiber seed generation unit, a sixth reflecting mirror and an optical filter, and weak seed light is output; the strong pump light is synchronized with the weak seed light output from the optical filter after entering the optical delay module; in the short-optical-path power amplification module, weak seed light and strong pump light output by the light delay module are combined at a dichroscope and are strictly coaxial, and then are focused by a convex lens and enter a short-optical-path Raman frequency shifter. According to the invention, the oscillation starting threshold of the Raman frequency shifter is greatly reduced by utilizing seed light-induced stimulated radiation.

An aim is to provide an optical communication system and an optical communication method for transmitting a wavelength-multiplexed signal whose signal quality on a reception side is adjusted to desired quality. A first optical transmitter outputs a first optical signal of a first wavelength at a first transmission intensity. A second optical transmitter outputs a second optical signal of a second wavelength longer than the first wavelength at a second transmission intensity lower than the first transmission intensity. A multiplexer outputs a third optical signal obtained by multiplexing the first optical signal and the second optical signal to an optical transmission line. The intensity of the first optical signal transmitted through the optical transmission line is reduced by inter-signal stimulated Raman scattering. The second optical signal transmitted through the optical transmission line is amplified by the inter-signal stimulated Raman scattering of the first optical signal.

An optical arrangement for adjusting the wavelength of light, comprising: a first light source arranged to generate a first beam of light at a first wavelength; a second light source arranged to generate seed light at a second wavelength; a first Raman shifting medium arranged to receive the light from the first light source in combination with the seed light from the second light source, and to produce, by stimulated Raman scattering, output light at the second wavelength and having temporal properties determined by those of the first beam of light; a third light source arranged to generate seed light at a third wavelength; and a second Raman shifting medium arranged to receive the output light from the first Raman shifting medium in combination with the seed light from the third light source, and to produce, by stimulated Raman scattering, output light at the third wavelength and having temporal properties determined by those of the output light from the first Raman shifting medium; wherein the third wavelength is greater than the second wavelength, and the second wavelength is greater than the first wavelength; wherein the frequency difference between the first beam of light and the seed light from the second light source is a frequency difference where the first Raman shifting medium exhibits Raman gain; and wherein the frequency difference between the output light from the first Raman shifting medium and the seed light from the third light source is a frequency difference where the second Raman shifting medium exhibits Raman gain. Also provided is a corresponding method of adjusting the wavelength of light.

The application discloses a kind of multi-channel wide spectrum scattered light energy simultaneous measurement device and method, wherein multi-channel wide spectrum scattered light energy simultaneous measurement device mainly includes target chamber, spectrometer, gate camera and multiple scattered light imaging light paths.The present multi-channel wide spectrum scattered light energy simultaneous measurement device and method are used, by setting multiple scattered light imaging light paths, realize multi-channel measurement capability, can accurately give the energy of different wavelengths and total energy, realize the simultaneous measurement of stimulated brillouin scattering and stimulated raman scattering scattered light, provide more reliable diagnostic techniques for the scattered light generated by laser target shooting;At the same time, the present measurement method greatly reduces the use of energy probe, not only saves cost, but also improves diagnostic efficiency;And, the present measurement method can be used not only for high-energy scattered light measurement, but also for other light source intensity analysis and comparison.

The application belongs to the field of laser frequency conversion, and particularly relates to a gas stimulated Raman frequency conversion device with a double-gas-chamber structure, wherein a pump laser source, a 45-degree mirror and a focusing lens are arranged outside a Raman cell A, two ends of the Raman cell A are respectively provided with a Raman cell window A and a Raman cell window C, the inside of the Raman cell A is provided with a Raman cell B, one end of the Raman cell B is connected with one end of the Raman cell A and shares the Raman cell window A, and the other end of the Raman cell B is provided with a Raman cell window B; one end of the Raman cell A in the length direction is provided with a plane mirror located below the Raman cell B, the other end of the Raman cell A in the length direction is provided with a concave mirror and a plane mirror, and the concave mirror and the plane mirror at the end are arranged in the up-down direction along the height direction of the Raman cell A.

A stimulated Raman photothermal (SRP) microscope for imaging a sample. A first optical source without an optical resonator emits a pump beam. A second optical source emits an intensity-modulated Stokes beam. The Stokes beam is combined with the pump beam to form a combined beam. The combined beam is directed to the sample to induce a thermal effect caused by the stimulated Raman process. A third optical source emits a probe beam, the probe beam is directed to the sample. An optical detector detects modulation of the probe beam after modulation by the sample to measure an SRP signal. Because noise in the pump and Stokes beam do not significantly effect the measurements from the probe beam, these beams can use a high-powered optical parametric amplifier (OPA) source for improved sensitivity and imaging speed compared to SRP microscopes using an optical parametric oscillator (OPO) source.