150results about "Raman/scattering spectroscopy" patented technology

A composite optical filter may include a substrate, a communal optical filter, one or more first optical filters, and one or more second optical filters. The communal optical filter may be formed on a first surface of the substrate. The one or more first optical filters may be formed on one or more first portions of a second surface of the substrate and may be configured to pass light associated with a first wavelength. The one or more second optical filters may be formed on one or more second portions of the second surface of the substrate and may be configured to pass light associated with a second wavelength.

A method and a system have been developed to calibrate the total spectral radiance factor (TSRF) obtained from different instruments. A broad-band light source covering the whole visible range as well as the fluorescence excitation wavelengths is used to measure the TSRF, and one or more narrow-band light sources outside of the fluorescent band is used to measure fluorescent spectral radiance factor (FSRF) separately. After that, the TSRF is adjusted by the FSRF, and a calibrated TSRF can be obtained. This can be applied to characterize Optical Brightening Agents (OBAs) as well as other fluorescent materials without requiring any moving part of the instrument to do the calibration.

A portable device which detects a Raman signal from an analyte of interest contained in or suspected to contain in a sample. The portable device includes a laser source in optical communication with a dichroic mirror, an objective lens optically positioned to consolidate the laser from the laser source to the sample, (i) a pair of rotatable filters in optical communication with the dichroic mirror and an optical transmission module or (ii) a first band-pass filter in optical communication with the dichroic mirror and an optical transmission module, and a detector optically positioned to receive and detect a Raman signal produced from the laser incident on the analyte of interest. The dichroic mirror directs the laser from the laser source to the sample through the objective lens and transmits any Raman signal from the sample toward the detector. The detector is absent of a spectrometer and detects the Raman signal.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

A time measurement apparatus 10 includes a TAC circuit 12, a measurement gate 11, a control unit 14 for setting a gate dead time, which is a time during which the measurement gate 11 is set to be in the second state, in the measurement gate 11, and the control unit 14 for deriving and outputting time information related to the detection signal based on a measurement signal output from the TAC circuit 12, and the control unit 14 functioning as a setting unit sets a time, which is an integral multiple of a repetition period of fluorescence detected by the detector 4 and is longer than a dead time of the TAC circuit 12 itself, in the measurement gate 11 as a gate dead time.

High-throughput hyperspectral imaging systems are provided. According to an aspect of the invention, a system includes an excitation light source; an objective that is configured to image excitation light onto the sample, such that the excitation light causes the sample to emit fluorescence light; a channel separator that is configured to separate the fluorescence light into a plurality of spatially dispersed spectral channels; and a sensor. The excitation light source includes a light source and a plurality of lenslet arrays. Each of the lenslet arrays is configured to receive light from the light source and to generate a pattern of light, and the patterns of light generated by the lenslet arrays are combined to form the excitation light. The objective is configured to simultaneously image each of the patterns of light to form a plurality of parallel lines or an array of circular spots at different depths of the sample.

The present disclosure relates to a novel liquid crystal assembly for use in multispectral imaging systems. In particular, the liquid crystal assembly includes an engineered polarizer and a liquid crystal. In various aspects, the liquid crystal assembly takes advantages of short-comings found in conventional polarizers and liquid crystals requiring complex temperature regulation.

The present application relates to a kind of based on micro-nano fiber and scattering medium substrate coding element and speckle spectrometer.The coding element includes sealed cavity, micro-nano fiber and scattering medium substrate;Including drawstring transition zone and waist zone in the body of micro-nano fiber, drawstring transition zone forms leaky mode, waist zone generates evanescent field, the body of micro-nano fiber penetrates sealed cavity, so that drawstring transition zone and waist zone are located in sealed cavity;Scattering medium substrate is fixed in sealed cavity, and the waist zone of micro-nano fiber is overlapped on scattering medium substrate, scattering medium substrate is used to receive evanescent field and is coupled leaky mode to obtain scattering pattern information;Wherein, in the direction parallel to waist zone, scattering medium substrate includes more than two scattering zones, and the light transmittance of each scattering zone is different, and the difference between maximum light transmittance and minimum light transmittance is 20%~80%.Speckle spectrometer using the coding element of the present application has the advantages of small size, low cost while high spectral resolution.

A fluorimeter reference cell which comprises a first material which emits an absorption spectrum when an excitation light is incident upon the first material. The absorption spectrum has a defined cha

In one embodiment, an apparatus includes a needle that includes a substantially cylindrical shaft and a lumen, which includes a hollow space located within the shaft. The shaft includes a tip located at an end of the shaft, where the tip includes an opening. The apparatus also includes an optical fiber that includes an end face. The end face and at least a portion of the optical fiber are located within the lumen. The optical fiber is configured to transmit a pump-Stokes beam of light along the optical fiber toward the tip of the needle and to the end face, where the pump-Stokes beam is emitted from the end face and directed to a sample. The apparatus further includes a catheter that includes a catheter connector and a catheter tube having a substantially cylindrical shape, where the catheter tube surrounds at least a portion of the needle shaft.

The application provides an angle-resolved Raman device and an analysis equipment, and relates to the technical field of optical machinery. During use of the device, the parallelism of the light path of a closed Raman light path system with a first direction can be adjusted by using an angle-resolved positioning mechanism first, the closed Raman light path system can be moved by using a second fine adjustment mechanism, and thus the adjustment of the parallelism is assisted to complete, so that the position of a light spot on a sample is unchanged when an incident angle alpha of the closed Raman light path system to the sample changes, and thus the in-situ property is ensured. After the adjustment of the tilt angle is completed, the position of the light path of the closed Raman light path system on a first plane can be adjusted by using a first fine adjustment mechanism, so that the light path is coaxial with an axis of the first rotating mechanism, and the in-situ property during rotation of a microscope lens is ensured.

In one embodiment, an apparatus includes a needle that includes a substantially cylindrical shaft and a lumen. The lumen includes a hollow space located within the shaft, and the shaft includes a tip located at an end of the shaft. The apparatus also includes an optical fiber that includes an end face, where the end face and at least a portion of the optical fiber are located within the lumen. The optical fiber is configured to transmit a pump-Stokes beam of light along the optical fiber toward the tip of the needle and to the end face, where the pump-Stokes beam is emitted from the end face and directed to a sample located in the lumen. The pump-Stokes beam is configured to produce a Raman signal by coherent Raman scattering of the pump-Stokes beam while propagating through the sample.

An imaging system (400) includes an imager (410) having first and second light sensors (465, 467), the first light sensor (465) being configured to record light in a first wavelength range and the second light sensor (467) being configured to record light in a second wavelength range, an alignment mechanism (440) configured to be attached to the imager (410), an illumination source (450) configured to generate a supercontinuum light beam, and a light shaping mechanism (452) configured to transform the supercontinuum light beam into a linear strip of light (450B). The alignment mechanism (440) is configured to adjust a position of the light shaping mechanism (452) so that a back scattered light (710), resulting from a scattering of the linear strip of light (450B) from a target (620), has an intensity above a given minimum for each of the first and second light sensors (465, 467).

To provide a spectroscopic analysis system, etc. that makes it possible to appropriately conduct measurement even when measurement time, etc is limited.SOLUTION: A spectroscopic analysis system 100 comprises: an operation panel 21 that accepts the upper-limit value of measurement time of a spectroscopic spectrum and / or the lower-limit value of measurement accuracy as a user set condition regarding the spectroscopic spectrum measurement of a sample; and a control unit 32 that derives a prescribed recommended measurement condition that would satisfy the user set condition and causes a display unit 22 to show the recommended measurement condition. The recommended measurement condition is at least one of the wavelength range of light that is used for measuring spectroscopic spectra, the wavelength sampling interval of light, the slit width of diffraction gratings 2a, 8a of a spectroscope that spectrally separates light, and the wavelength sweep speed of light.SELECTED DRAWING: Figure 1

Flowcells and Raman analysis systems provide improved signal collection dynamics through increased solid-angle geometries and improved numerical aperture for near-diffraction-limited performance. A combined excitation / collection beam passes through a first optical material, a sample conduit and a second optical material. A concave reflective aspheric surface focuses and re-collimates the combined beam to and from a region of the sample within the conduit. The optical materials may comprise separate windows or may integrally form sidewalls the conduit. The reflective surface may be spaced apart from the second window or may be integrally formed with the second optical material. The focused region in the sample may approximate a point or a line, and at least a portion of the interior wall of the conduit may be reflective, causing the combined beam to pass through the sample region more than once to enhance collection efficiency.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The invention relates to a Raman spectroscopy device for measuring the concentration of a gas (22). The essential aspect of the invention is that the Raman spectroscopy device (30) in the gas measurement chamber (20, 56) has a sleeve-shaped component (40), wherein the sleeve-shaped component (40) has a cavity (41) inside, and wherein the gas to be measured is conductable in the longitudinal direction essentially through the cavity (41) of the sleeve-shaped component (40). that the sleeve-shaped component (40) has openings (42) on its outer surface through which the laser light of the high-power laser (14) can be directed into or out of the cavity (41), that the sleeve-shaped component (40) has at least one aperture section (43) which projects into the cavity (41), that the sleeve-shaped component (40) has an outlet opening (44) on its outer surface for supplying Raman scattering to the spectral analysis unit (38), that the aperture section (43) is geometrically arranged between the openings (42) through which laser light enters and exits the cavity (41) and the outlet opening (44), that the aperture section (43) is designed in the form of at least two aperture flaps directed inwards into the component.

A microscopic confocal fluorescence spectrometer is provided. The microscopic confocal fluorescence spectrometer includes an optical system and an instrument body. By adopting an open microscopic fluorescence light path, a lens body part of an existing spectrometer microscope is omitted, so that the space behind an objective lens is significantly increased. By reasonably arranging a laser, a spectrum detection device and other components, the space on the side of the objective lens is further increased, and the occupied space of the optical system is reduced, so that the overall volume of the instrument is reduced, and the adaptability of the instrument is further improved. In addition, the spectrum detection device is arranged outside the second shell to facilitate the heat dissipation of the spectrum detection device, so that the heat stability and working reliability of the instrument are further improved.

A multi-dispersive spectrometer is provided in which the spectrometer comprises an optical system configured to direct an excitation signal from an excitation light source toward a sample, receive a spectroscopy signal from the sample, and direct the spectroscopy signal toward the detector. The optical system comprises a movable optical component adapted to move the spectroscopy signal relative to at least one sensor of the detector and the detector is adapted to detect a plurality of discrete shifted spectroscopy signals. A method of obtaining a Raman spectrum from a sample is also provided. The method comprises directing an excitation signal from an excitation light source toward a sample; receiving a spectroscopy signal from the sample; and directing the spectroscopy signal toward a detector, wherein the spectroscopy signal is moved relative to at least one sensor of the detector to provide a plurality of discrete shifted spectroscopy signals.

In some instances, a system for rapid spectral unmixing using spectrally interpolated background reduction (SIBR) is provided. The system comprises a device comprising an excitation light source and a detector. The system further comprises a control system configured to: obtain a plurality of excitation spectra for a plurality of fluorophores that are used to stain a sample; determine a plurality of chosen wavelengths and a plurality of chosen light intensities for the plurality of fluorophores; obtain a first detection of the sample based on using a set of first wavelengths from the plurality of chosen wavelengths and a set of first light intensities; obtain a second detection of the sample based on using a set of second wavelengths from the plurality of chosen wavelengths and a set of second light intensities; and output a SIBR quantity.

In one embodiment, a system includes a pump light source configured to produce a pump beam of light at a pump frequency and a Stokes light source configured to produce a Stokes beam of light at a Stokes frequency. The pump and Stokes frequencies are offset by a frequency offset Ω, and the pump and Stokes beams are directed to an object. The system further includes one or more optical elements configured to collect a Raman signal produced by coherent Raman scattering of the pump and Stokes beams of light at the object. The system also includes an optical receiver configured to detect the Raman signal. The optical receiver includes a probe light source configured to produce a probe beam of light at a probe frequency and an optical detector configured to coherently mix the Raman signal with the probe beam of light to produce a corresponding photocurrent signal.

A data processing method and system for a spectrum sensor, a medium and a device are provided.SOLUTION: A data acquisition step of acquiring a reflection signal or an excitation signal of an imaging region generated during light irradiation, a light filtering step of passing the acquired reflection signal or excitation signal through a periodic pixel-level light filter structure set on a sensor surface to generate a mosaic image, and a processing step of respectively selecting a detection point and a reference point from corresponding positions of the mosaic image based on a detection point candidate region and a reference point candidate region pre-divided in the imaging region, and respectively calculating and obtaining spectrum data of the detection point and spectrum data of the reference point. The interference component including the non-analysis object in the collected signal is removed, and the acquisition accuracy of the spectrum data can be improved.SELECTED DRAWING: Figure 9

A multiple-wavelength reflective Raman probe includes a first parabolic mirror, a second parabolic mirror, a third parabolic mirror, and a fourth parabolic mirror. The first parabolic mirror receives a laser light, and reflects as a collimated light beam. The second parabolic mirror receives the collimated light beam, and condenses to a convergent light beam. The third parabolic mirror has a through hole. The convergent light beam passes through the through hole, and is focused on the sample detecting position. The third parabolic mirror also collects the light reflected by the sample detecting position and reflects as a collimated detection light beam. The fourth parabolic mirror collects the detection light beam, and condenses to a detection output light beam. The detection output light beam is focused at a focal point of the fourth parabolic mirror to be collected by a spectrometer.

Non-invasive in vivo measurement, by Raman spectroscopy, of analyte e.g. glucose present in e.g. interstitial fluid in the skin of a subject. A plurality of vertical-cavity surface-emitting lasers (VC

Disclosed is a modularized-reconfigurable long-term in-situ monitoring device and a monitoring method. The monitoring device comprises a fixed component. The fixed component is provided with a physical and chemical parameter sensor interface, a multi-channel Raman detection system, a camera system, an ocean current recording system, a control system, a power supply system, a lighting system, a floating body and an optical communication system. A releaser is arranged in the middle of the top of the fixed component. The releasing end of the releaser is connected with one end of a connecting rod penetrating through the fixed component, and the other end of the connecting rod is connected with a bottom counterbalance weight through a manual releaser. The working efficiency is improved, the operation risk is reduced, and the positioning precision is high.

The present invention relates to an apparatus and method for non-invasive in vivo measurement, by Raman spectroscopy, of glucose present in interstitial fluid in the skin of a subject. The apparatus c

Provided is a SERS substrate structure for chemical detection including a plasmonic nanofilm having a first functional group on a surface, a metal ion disposed on the surface of the plasmonic nanofilm, a target molecule disposed on the surface of the plasmonic nanofilm and having a second functional group, and a plasmonic nanostructure which is disposed on the surface of the plasmonic nanofilm with the metal ion and the target molecule interposed therebetween to cause surface-enhanced Raman scattering (SERS), wherein the first functional group and the second functional group may form a coordination compound through the metal ion.

An imaging system comprising: a light source configured to provide wavelength-swept emission of light; a sample delivery channel coupled to the light source and arranged to be coupled to an object to be imaged to direct light from the light source towards said object; a reference channel coupled to the light source for receiving light therefrom; a sample receiving channel arranged to be coupled to the object to be imaged for receiving sample light from the object; a detector coupled to the sample receiving channel for receiving sample light, and coupled to the reference channel for receiving reference light, wherein the detector is arranged to combine the received sample light with the reference light to provide a combined light signal comprising one or more components at a beat frequency between sample light and reference light; and a lock-in amplifier arranged to: (i) receive a detection signal based on the combined light signal and formed of one or more components at different frequencies, (ii) receive a selection signal at a selection frequency, and (iii) to provide one or more output signals indicative of a component of the detection signal at the selection frequency.

A system and method are disclosed for quantifying and removing stray light incident on a Raman optical sensor from a Raman spectrum. One or more background noise measurements are performed by a Raman analyzer, with the laser disabled during the background noise measurements. One or more conventional signal acquisition measurements are then performed. The background noise measurements and the conventional signal acquisition measurements are then processed, resulting in a clean spectrum with significantly reduced background noise. In some embodiments, background noise measurements are performed before and after the conventional signal acquisition measurements, and these noise measurements are averaged.

Provided herein are devices, systems, and methods for medical imaging. In particular, provided herein are devices, systems, and methods for real-time simultaneous multi-wavelength fluorescence and color imaging, e.g., configured for use during fluorescence guided diagnostic and surgery (FGS) applications.