218results about "Nanoparticle analysis" patented technology

Methods of studying characteristics of a sample over time are disclosed. The method includes irradiating a sample containing particles with a light beam to generate scattered light by interaction of the light beam with the sample, obtaining measurements of the scattered light over a measurement period divided into a plurality of shorter sub-processes, classifying each sub-process, and determining sample particle characteristics for the sub-processes. The method may also include transforming each sub-process prior to classifying each sub-process. Determining sample particle characteristics for the sub-processes may be performed prior to classifying each sub-process. The method may also include determining a change in the sample particle characteristic over time in the sub-process.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sensea fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

A flow nanocytometer for measuring extracellular vesicles (EVs) may include a flow cell for receiving a core stream carry at least one EV, at least one excitation source module for outputting a light beam to interrogate a portion of the core stream, a detection module for detecting the light beam, and a processor communicatively coupled to a memory, the at least one excitation source, and the detection module. The processor may execute a method including receiving a plurality of electronic signals from the detection module; detecting an intensity of a polycystic kidney disease (PKD) associated protein expressed by the EVs based on the plurality of electronic signals; determining a quantity of the EVs based on the plurality of electronic signals; and determining a severity or a prognosis of polycystic kidney disease in the subject, based on the intensity of the PKD associated protein and the quantity of the EVs.

Methods and systems for analyzing lipid nanoparticles using size exclusion chromatograph coupled with multi angle light scattering are disclosed.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

The invention relates to a device (100) for locating an individual particle (2) in a sample (3) having a plurality of regions (31), the particle being capable of emitting photons when it receives light, the device comprising: - a sample holder (110); - a light source arranged to produce an illumination field suitable for illuminating the sample placed on its holder, an intensity of the illumination field having a temporal and spatial variation such that the intensity has, in each region of the sample, a predetermined temporal pattern different from the temporal patterns of the intensity in the other regions; - a detector (140) capable of converting photons emitted by the particle in response to the illumination thereof by the illumination field into a signal representative of the intensity of the illumination field received by the particle; - a computer (150) programmed to determine, on the basis of a temporal change in the signal derived from the detector and the temporal and spatial variation in the intensity of the illumination field, the region of the sample in which the particle is located.

A spectroscopic bioagent detection apparatus and method are provided. In one aspect, an optical detection system and method are used to identify and / or detect a virus or bacteria by using a microscope and measuring vibrational motion or phonons of the virus or bacteria. A further aspect of the present apparatus and method include automatically optically measuring vibrational motion or phonons of a target virus or bacteria, substantially in real-time, in vivo or in situ, automatically filtering out undesired background and living cell signals, and automatically identifying a characteristic of the virus or bacteria.

The invention describes a method for detecting biological agents suspended in the air in real time, comprising: emitting, towards an air sample, a beam of monochromatic polarized light whose wavelength corresponds to an absorption maximum of a biological molecule; receiving, at different scattering angles, the scattered light beam after passing through the sample; determining that there are particles in the sample that contain the biological molecules of interest if the intensity of the scattered light has a substantially higher peak than the rest of the scattered light; estimating the amount of said biological molecules according to the amplitude of said peak; and estimating the size of the particles containing the biological molecules as a function of the scattering angle where said peak was detected. The invention also describes a system for carrying out said procedure.

The invention belongs to the technical field of analysis of single particles, and particularly discloses a method for detecting the atomic number ratio of multiple elements in a single particle in a nano material, which can synchronously collect ion signals of all the elements in the single particle, accurately measure the intensity of each element in a transient signal, and improve the detection accuracy. A novel data processing method, namely a statistical distribution fitting method, is adopted, so that the real and accurate multi-element atomic ratio is calculated, and the inherent defects of the traditional SP-ICP-MS and TEM technologies are overcome; meanwhile, the problem that part of nano-particles are unstable in a water phase is solved through an organic phase oxygen adding and sampling method.

Systems and methods for analyzing fluid samples containing nanoparticles for the determination of nanoparticle count and nanoparticle elemental composition are described. In an aspect, a system embodiment includes, but is not limited to, a remote sampling system configured to receive a fluid sample containing nanoparticles, the remote sampling system including a particle counter configured to determine a number of nanoparticles present in the fluid sample via light scattering detection; a sample analysis system configured to receive the fluid sample from the remote sampling system, the sample analysis system including a mass spectrometer configured to determine an elemental composition of the nanoparticles present in the fluid sample; and a system controller communicatively configured to provide a distribution of the nanoparticles in the fluid sample by specific element type based on an output from each of the mass spectrometer and the particle counter.

Provided is a portable biosensor that includes a sample filter cartridge, a filter collector, an optical sphere, an electromagnetic radiation emitter, a photo-detector, a processor, a signal display, a vacuum pump, and a power supply. The sample filter cartridge selectively removes small molecules to minimize spectral interference in the detection signal. The sample is concentrated onto the filter collector and subjected to illumination by the electromagnetic radiation emitter, producing Raman-scattering. The optical sphere collects and distributes the Raman-scattering shifts, which then pass through a spectral filter to produce spectral filtered scattering, which is then reflected by the concave holographic flat-field grating onto the photo-detector. The data is displayed graphically to provide the Raman-scattering shift data. The data is compared with a database for sample identification. The device is contained within a housing that is small enough to be easily transported for field use.

High throughput single molecule tracking (htSMT) systems and methods are described in which the htSMT workflow is adapted to characterize the contribution of known and new pathways to interaction networks in living cells, such as protein signaling interaction networks.

In semiconductor manufacture requiring ultra high purity water, particles of specified sizes are detected using acoustic particle detection capable of detecting nanoparticles. A sensor unit receives a flow of water, with the pressure and rate of flow to the sensor controlled closely. In each sensor unit is a measurement cell to receive a continuous flow of water, with a transducer module engaged with the cell to deliver an acoustic pulse at a known level of intensity as the liquid flows through the cells, the acoustic pulse acting on the liquid at a focus zone to cause cavitation in the event a particle of size detectable at that intensity is present. A waveform emitter / receiver connected to the sensor unit sends high-frequency, high-voltage signal to the transducer module to cause the acoustic pulse, and it also receives a waveform echo indicating a cavitation event. The system is controlled by a microprocessor.

The optical interrogation device can have a substrate; an insulating layer supported by the substrate; a plasmonic layer supported by the substrate via the insulating layer; a cavity extending across both the plasmonic layer and the insulating layer to a bottom adjacent the substrate, the cavity being sized to receive a particle; and a layer of 2D material covering the substrate and defining the bottom of the cavity. The process of optically interrogating the particle can include the layer of 2D material attracting the particle into the cavity across the upper end, and, while the particle is in the cavity, acquiring an optical signal including a spectral signature of the particle.

Embodiments relate to a biosensor. The biosensor includes an electrochemical system having a generator electrode, a collector electrode, and electrolyte. The generator electrode includes a particle capture region having an aspect ratio that is greater than 1. The particle capture region is a portion of the generator electrode configured to attract a particle supported within the electrolyte. The electrochemical system is operated so as to bias one of the generator electrode or the collector electrode below its formal potential while biasing the other electrode above its formal potential. The biosensor includes a processing module configured to measure electric current generated by the electrochemical system.

A system (2) for concentrating a substance dispersed in a fluid is provided. The system comprises a nanofluidic structure (14). The nanofluidic structure (14) comprises at least one dead-end nanochannel (18) extending in an extension direction from an entrance (22) to a dead-end (24). The dead-end nanochannel has a length in the extension direction and a width perpendicular to the extension direction, the length being greater than the width. The system further comprises an electrical apparatus (5) arranged to apply an electric field over the nanofluidic structure which is at least partially aligned with the extension direction of the dead-end nanochannel, to encourage a concentration of the substance to increase at the entrance and / or the dead-end of the dead-end nanochannel.

A particle measuring device includes a mount unit fixing a flow cell and a resonance unit disposed behind the mount unit. The resonance unit forms a hollow portion that is open forward and rearward. The mount unit includes a fixing module including first and second fixing modules positioned in front of the resonance unit and horizontally disposed with the flow cell interposed therebetween and a bridge module including upper and lower bridge modules respectively coupled to an upper end and a lower end of the fixing module. Each of the first and second fixing modules includes a fixing body extending in an up-down direction, a fixing body upper protrusion formed on an upper end of the fixing body and coupled to the upper bridge module, and a fixing body lower protrusion formed on a lower end of the fixing body and coupled to the lower bridge module.

Chemical mechanical polish processes on surfaces of semiconductor devices comprising tungsten layers can create nano-sized particles of tungsten. These particles can create manufacturing yield reductions. These particles can also be difficult to detect optically. Etched surfaces coated with a layer of material that can provide optical detection enhancement provide an ability to optically detect nano-sized particles.

The present invention relates to a system (1) and a method for coating a surface (3a) of an object (3) with a spray coating tool (2) spraying atomised coating particles (10) onto. A conditioning hood (20) is applied defining a spray chamber. In operation the spray coating tool (2) is mounted at a coating tool mounting end (21), and an output end (22) is positioned in proximity of the surface (3a) of the object (3) to be coated. The spray coating tool (2) is operated to spray atomised coating particles (10) which travel from the coating tool (2), through the spray chamber and the output end (22) to the surface (3a) of the object (3). A carrier gas is injected that in operation carries the atomised coating particles (10) to the surface (3a) of the object (3).

Provided is a pore device which can be stored for a long period of time without impairing reliability. A void device (100) is provided with a device body (150) and a sealing member (160). The device main body (150) has a first space (122) and a second space (124) that communicate with each other through the aperture (104), and also has an injection port (152) for injecting the electrolyte solution (5) into the first space (122) and the second space (124). A hydrophilic group (154) is applied to the inside of the device main body (150). The sealing member (160) seals the injection port (152) in a state in which the first space (122) and the second space (124) are filled with the electrolyte solution (5).

The present disclosure is directed towards improved systems and methods for large-scale production of nanoparticles used for delivery of therapeutic material. The apparatus can be used to manufacture a wide array of nanoparticles containing therapeutic material including, but not limited to, lipid nanoparticles and polymer nanoparticles. In certain embodiments, continuous flow operation and parallelization of microfluidic mixers contribute to increased nanoparticle production volume.

Disclosed herein are hyperspectral acquisition systems for generating and recording hyperspectral profiles of nanoparticles and methods of using the systems. The hyperspectral acquisition systems include two systems: (1) a hyperspectral acquisition system (e.g., hyperspectral imaging system) for generating and recording the hyperspectral profiles and (2) a hyperspectral analysis system for assigning identifications using spectral features.

A method for investigating sample characteristics that change over time is disclosed. The method includes illuminating a sample having particles with a light beam such that interaction of the light beam with the sample produces scattered light, obtaining measurements of the scattered light over a measurement period divided into a plurality of shorter sub-runs, classifying each sub-run, and determining sample particle characteristics for the sub-run. The method may further include transforming each sub-run before classifying it. Determining the sample particle characteristics for the sub-run may occur before classifying it. The method may also include determining the change in sample particle characteristics over time for the sub-run.

Arrangement for measuring particles (20) entrained in a liquid, in particular microplastic particles. The arrangement comprises: a measuring channel (14) with an inlet for supplying the liquid; an illumination unit (13) for emitting a measuring beam path (16) that impinges on a measuring volume (19) of the liquid located in the measuring channel (14) and excites at least one particle (20) located in the measuring volume (19) to emit scattered light; a spectrometer (18) for detecting an inelastic scattered light spectrum of the emitted scattered light; detection optics (15) configured to transmit portions (16a, 16b) of the scattered light that impinge on the detection optics (15) along a detection direction to the spectrometer (18); and an evaluation unit (21) for identifying at least one material type of the particle (20) located in the measuring volume (19) on the basis of the scattered light spectrum recorded by the spectrometer (18).According to the invention, the angle between an optical axis of the measuring beam path (16) and the detection direction lies in a range between 45° and 135°. The signal quality is improved by aligning the detection direction relative to the measuring beam path.

Disclosed is a method of differentiating empty capsids from full capsids or loaded and unloaded non-viral gene therapy vectors in a viral preparation. The method comprises the steps of: a) providing a preparation of viral particles or gene therapy vectors; b) subjecting the preparation to interferometric scattering mass cytometry (ISCAMS) in an interferometric scattering microscope to generate mass distribution data of the viral particles; c) determining from the mass distribution data the level of empty capsids and capsids containing genomes or the level of loaded and unloaded vectors in the viral particles.

The invention provides methods of optically detecting an analyte in a medium as the analyte moves with respect to a nanopore. The analyte may be, for example, a biological molecule such as a polynucleotide or polypeptide. Systems and apparatuses for carrying our such methods are also provided.

The present disclosure provides systems, methods, and kits for improving the characterization (including quality control) of flow cytometers. In one embodiment, a system for characterizing a flow cytometer includes a first standard particle reagent (the first standard particle reagent includes a first particle mixture) and a second standard particle reagent (the second standard particle reagent includes a second particle mixture, the particles having a fluorescent dye). The systems, methods, and kits of the present invention offer significant advantages over currently available fluorospheres used for quality control of flow cytometers prior to evaluating nanoparticles (e.g., EVs).

The invention relates to an optical flow cytometer for fluorescence and scatter measurements, comprising: - a non-coherent light source intended to generate an illumination beam; - a beam truncation device comprising at least a first passage allowing a first portion of the illumination beam having a first divergence to pass, the first portion being dedicated to scatter measurements, and a second passage allowing a second portion of the illumination beam having a second divergence to pass, the second portion being dedicated to fluorescence measurements, the second divergence being greater than the first divergence, the first passage and the second passage being separated by a region of the beam truncation device blocking the illumination beam; - at least one focusing lens for focusing the first portion and the second portion of the illumination beam to a flow cell comprising an optical interrogation zone; - a flow cell intended to contain particles flowing through the optical interrogation zone; - a scatter detector for receiving light scattered from the first portion of the illumination beam when a particle passes through the optical interrogation zone; - a fluorescence detector for receiving fluorescence emitted by a particle passing through the optical interrogation zone.

Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.