131results about "Nanoparticle analysis" patented technology

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sensea fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Methods and systems for analyzing lipid nanoparticles using size exclusion chromatograph coupled with multi angle light scattering are disclosed.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

The invention relates to a device (100) for locating an individual particle (2) in a sample (3) having a plurality of regions (31), the particle being capable of emitting photons when it receives light, the device comprising: - a sample holder (110); - a light source arranged to produce an illumination field suitable for illuminating the sample placed on its holder, an intensity of the illumination field having a temporal and spatial variation such that the intensity has, in each region of the sample, a predetermined temporal pattern different from the temporal patterns of the intensity in the other regions; - a detector (140) capable of converting photons emitted by the particle in response to the illumination thereof by the illumination field into a signal representative of the intensity of the illumination field received by the particle; - a computer (150) programmed to determine, on the basis of a temporal change in the signal derived from the detector and the temporal and spatial variation in the intensity of the illumination field, the region of the sample in which the particle is located.

The invention describes a method for detecting biological agents suspended in the air in real time, comprising: emitting, towards an air sample, a beam of monochromatic polarized light whose wavelength corresponds to an absorption maximum of a biological molecule; receiving, at different scattering angles, the scattered light beam after passing through the sample; determining that there are particles in the sample that contain the biological molecules of interest if the intensity of the scattered light has a substantially higher peak than the rest of the scattered light; estimating the amount of said biological molecules according to the amplitude of said peak; and estimating the size of the particles containing the biological molecules as a function of the scattering angle where said peak was detected. The invention also describes a system for carrying out said procedure.

The invention belongs to the technical field of analysis of single particles, and particularly discloses a method for detecting the atomic number ratio of multiple elements in a single particle in a nano material, which can synchronously collect ion signals of all the elements in the single particle, accurately measure the intensity of each element in a transient signal, and improve the detection accuracy. A novel data processing method, namely a statistical distribution fitting method, is adopted, so that the real and accurate multi-element atomic ratio is calculated, and the inherent defects of the traditional SP-ICP-MS and TEM technologies are overcome; meanwhile, the problem that part of nano-particles are unstable in a water phase is solved through an organic phase oxygen adding and sampling method.

Systems and methods for analyzing fluid samples containing nanoparticles for the determination of nanoparticle count and nanoparticle elemental composition are described. In an aspect, a system embodiment includes, but is not limited to, a remote sampling system configured to receive a fluid sample containing nanoparticles, the remote sampling system including a particle counter configured to determine a number of nanoparticles present in the fluid sample via light scattering detection; a sample analysis system configured to receive the fluid sample from the remote sampling system, the sample analysis system including a mass spectrometer configured to determine an elemental composition of the nanoparticles present in the fluid sample; and a system controller communicatively configured to provide a distribution of the nanoparticles in the fluid sample by specific element type based on an output from each of the mass spectrometer and the particle counter.

In semiconductor manufacture requiring ultra high purity water, particles of specified sizes are detected using acoustic particle detection capable of detecting nanoparticles. A sensor unit receives a flow of water, with the pressure and rate of flow to the sensor controlled closely. In each sensor unit is a measurement cell to receive a continuous flow of water, with a transducer module engaged with the cell to deliver an acoustic pulse at a known level of intensity as the liquid flows through the cells, the acoustic pulse acting on the liquid at a focus zone to cause cavitation in the event a particle of size detectable at that intensity is present. A waveform emitter / receiver connected to the sensor unit sends high-frequency, high-voltage signal to the transducer module to cause the acoustic pulse, and it also receives a waveform echo indicating a cavitation event. The system is controlled by a microprocessor.

The optical interrogation device can have a substrate; an insulating layer supported by the substrate; a plasmonic layer supported by the substrate via the insulating layer; a cavity extending across both the plasmonic layer and the insulating layer to a bottom adjacent the substrate, the cavity being sized to receive a particle; and a layer of 2D material covering the substrate and defining the bottom of the cavity. The process of optically interrogating the particle can include the layer of 2D material attracting the particle into the cavity across the upper end, and, while the particle is in the cavity, acquiring an optical signal including a spectral signature of the particle.

Embodiments relate to a biosensor. The biosensor includes an electrochemical system having a generator electrode, a collector electrode, and electrolyte. The generator electrode includes a particle capture region having an aspect ratio that is greater than 1. The particle capture region is a portion of the generator electrode configured to attract a particle supported within the electrolyte. The electrochemical system is operated so as to bias one of the generator electrode or the collector electrode below its formal potential while biasing the other electrode above its formal potential. The biosensor includes a processing module configured to measure electric current generated by the electrochemical system.

A system (2) for concentrating a substance dispersed in a fluid is provided. The system comprises a nanofluidic structure (14). The nanofluidic structure (14) comprises at least one dead-end nanochannel (18) extending in an extension direction from an entrance (22) to a dead-end (24). The dead-end nanochannel has a length in the extension direction and a width perpendicular to the extension direction, the length being greater than the width. The system further comprises an electrical apparatus (5) arranged to apply an electric field over the nanofluidic structure which is at least partially aligned with the extension direction of the dead-end nanochannel, to encourage a concentration of the substance to increase at the entrance and / or the dead-end of the dead-end nanochannel.

The present disclosure is directed towards improved systems and methods for large-scale production of nanoparticles used for delivery of therapeutic material. The apparatus can be used to manufacture a wide array of nanoparticles containing therapeutic material including, but not limited to, lipid nanoparticles and polymer nanoparticles. In certain embodiments, continuous flow operation and parallelization of microfluidic mixers contribute to increased nanoparticle production volume.

A method for investigating sample characteristics that change over time is disclosed. The method includes illuminating a sample having particles with a light beam such that interaction of the light beam with the sample produces scattered light, obtaining measurements of the scattered light over a measurement period divided into a plurality of shorter sub-runs, classifying each sub-run, and determining sample particle characteristics for the sub-run. The method may further include transforming each sub-run before classifying it. Determining the sample particle characteristics for the sub-run may occur before classifying it. The method may also include determining the change in sample particle characteristics over time for the sub-run.

Disclosed is a method of differentiating empty capsids from full capsids or loaded and unloaded non-viral gene therapy vectors in a viral preparation. The method comprises the steps of: a) providing a preparation of viral particles or gene therapy vectors; b) subjecting the preparation to interferometric scattering mass cytometry (ISCAMS) in an interferometric scattering microscope to generate mass distribution data of the viral particles; c) determining from the mass distribution data the level of empty capsids and capsids containing genomes or the level of loaded and unloaded vectors in the viral particles.

The invention provides methods of optically detecting an analyte in a medium as the analyte moves with respect to a nanopore. The analyte may be, for example, a biological molecule such as a polynucleotide or polypeptide. Systems and apparatuses for carrying our such methods are also provided.

The present disclosure provides systems, methods, and kits for improving the characterization (including quality control) of flow cytometers. In one embodiment, a system for characterizing a flow cytometer includes a first standard particle reagent (the first standard particle reagent includes a first particle mixture) and a second standard particle reagent (the second standard particle reagent includes a second particle mixture, the particles having a fluorescent dye). The systems, methods, and kits of the present invention offer significant advantages over currently available fluorospheres used for quality control of flow cytometers prior to evaluating nanoparticles (e.g., EVs).

The invention relates to an optical flow cytometer for fluorescence and scatter measurements, comprising: - a non-coherent light source intended to generate an illumination beam; - a beam truncation device comprising at least a first passage allowing a first portion of the illumination beam having a first divergence to pass, the first portion being dedicated to scatter measurements, and a second passage allowing a second portion of the illumination beam having a second divergence to pass, the second portion being dedicated to fluorescence measurements, the second divergence being greater than the first divergence, the first passage and the second passage being separated by a region of the beam truncation device blocking the illumination beam; - at least one focusing lens for focusing the first portion and the second portion of the illumination beam to a flow cell comprising an optical interrogation zone; - a flow cell intended to contain particles flowing through the optical interrogation zone; - a scatter detector for receiving light scattered from the first portion of the illumination beam when a particle passes through the optical interrogation zone; - a fluorescence detector for receiving fluorescence emitted by a particle passing through the optical interrogation zone.

Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.

A real-time, in situ and automated technology and method for dynamic observation of airborne viruses has been developed, and its application for airborne viruses, using a model virus, MS2 bacteriophage, and SARS-COV-1 RNA, has been demonstrated. There is described a method of performing measurements using a digital in-line holographic microscope (DIHM). The method generally has: propagating a light beam through a pinhole, across a medium including particles, and to a sensor; acquiring, with the sensor, a plurality of holograms, the holograms including scattering information of the particles; and determining, from a reconstruction of the holograms, at least one of shape, size, intensity and phase of the particles from the scattering information of said particles.

A nanoparticle measurement device is disclosed. A nanoparticle measurement device according to one embodiment of the present invention may include: a flow cell forming a cellular hollow portion in which a liquid sample flows; a cell mount coupled to and supporting the flow cell; a piezoelectric sensor facing the flow cell and connected to the flow cell; and a mount cover located opposite the flow cell with the piezoelectric sensor in between and fixed to the cell mount.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

A detection system of a flow cytometer and a method of using the detection system are provided. The detection system comprises a light-emitting unit; a lens assembly comprising a focussing lens; and a light collection unit configured to collect light scattered from a nanoparticle at the interrogation point. The method comprises using the lens assembly to focus a laser beam emitted by the light-emitting unit at an interrogation point. A profile of the incident laser beam incident on the focussing lens has a first dimension parallel to the trajectory of the nanoparticle. A first ratio of a first diameter of the focussing lens to the first dimension of the incident laser beam is higher than a first threshold such that light fringes of the focussed laser beam at the interrogation point are suppressed. An intensity of the focused laser beam at the interrogation point is higher than a second threshold.

Aspects of the present disclosure include methods for determining and applying a data signal filter for detecting particles (e.g., small particles such as extracellular vesicles) in a particle analyzer. The method, according to certain embodiments, includes detecting light from particles in a flowstream with a light detection system, generating a data signal waveform in response to the detected light from the particles in the flowstream, determining characteristics of the data signal waveform, and calculating the data signal filter from the determined characteristics of the data signal waveform. In some embodiments, the method includes determining a trigger metric for detecting particles in a sample based on the filtered data signal waveform. Systems and integrated circuit devices (e.g., field programmable gate arrays) for implementing the subject methods are also described. Non-transitory computer-readable storage media are also provided.

A sample holder and a system for using such a holder in interferometric microscopy. The sample holder is designed such that the height of a sample chamber (302) is similar or smaller than the coherence length of the illuminating light (304), said sample chamber (302) having a floor (301) and a roof defining therebetween an internal height of the sample chamber. The sample chamber further has a lower interface formed between an inner space of the sample chamber and the floor and an upper interface formed between the inner space of the sample chamber and the ceiling. The internal height is in a viewable area of the sample chamber designed such that light from the reflection from the chamber lower interface (305) and light from the reflection from the chamber upper interface (307) is sufficiently out of phase such that destructive interference of the two reflections is achieved.

Systems, methods, and devices are described herein for detecting and / or monitoring target extracellular vesicles (“EVs”), e.g., to detect and / or monitor cancer treatment, such as breast cancer, in a subject. The methods can include obtaining a nano-plasmonic array including nanostructures configured to amplify one or more specific wavelengths of electromagnetic radiation, flowing a liquid sample over the nano-plasmonic array, optionally labeling target EVs captured on the nano-plasmonic array with one or more reporter groups, projecting electromagnetic radiation onto the labeled target EVs captured on the nano-plasmonic array, and capturing an image of the target EVs by receiving electromagnetic radiation emitted, scattered, or reflected by the labeled target EVs or by reporter groups on the labeled target EVs.

To provide a method for detecting lipid nanoparticles.SOLUTION: The present disclosure provides a method for detecting size, density, or both size and density of lipid nanoparticles. The detection can be performed using centrifugal field flow fractionation with a carrier solution containing monosaccharides.SELECTED DRAWING: Figure 1

A method of estimating a proportion of particles filled with material. The method includes: obtaining an image depicting a plurality of particles; generating a plurality of classes based on the plurality of particles; identifying at least one of the plurality of classes having a central portion with a greater density than a central portion of at least one other of the classes; and estimating a proportion of the plurality of particles that are filled with material based on identifying at least one of the classes having a central portion with high density.

The present invention provides an apparatus for characterising particles using nanoparticle tracking analysis (NTA). The apparatus comprises: a flow cell for containing a sample comprising a plurality of particles suspended in a fluid; a pump configured to provide a flow of the sample through the flow cell; a light source configured to illuminate the sample; an imaging system configured to collect light scattered or fluoresced by particles moving within the flow cell and within a detection region of the imaging system, and capture a video of the particles moving within the detection region; and a computer configured to process the video. The computer is configured to determine an estimated flow velocity of the sample through the flow cell. Determining the estimated flow velocity comprises performing 1-dimensional particle tracking in a direction perpendicular to an expected flow direction of the sample.

The present invention relates to a method for measuring the properties of multiple particles in a solution and an apparatus for performing the method, wherein the method comprises the following steps: providing a container containing a sample of the multiple particles in the solution, wherein the sample has a volume preferably between 0.1 μL and 15 μL; providing a monochromatic light source and a photodetector; transmitting light from the monochromatic light source into the container containing the sample; detecting the light emitted from the container using the photodetector; and determining the properties of the multiple particles in the solution contained in the sample based on dynamic light scattering (DLS) measurements.

An interferometric microscope (200, 300) comprising: a sample holder (207) configured to hold a sample; a light source (201) arranged to illuminate the sample in the sample holder; an optical detector (210) arranged to capture images of light scattered by a feature of the sample; an optical system comprising a plurality of optical elements (202, 203, 204, 206) arranged to direct an illumination beam of light from the light source to the sample holder and to collect light reflected by the sample and by the sample holder to form an image beam directed to the detector; an optical component (212, 313) arranged in the path of the image beam and configured to divide the light in the image beam into a plurality of beam portions, wherein the beam portions are propagating along a common optical beam path, wherein the beam portions interfere at the detector; and a processing unit (211) configured to determine, from a plurality of images captured by the detector, a parameter indicative of the absolute value of the optical field of the light scattered by the feature