316results about "Nanoparticle analysis" patented technology

A system for detecting plastics, macro-plastics, micro-plastics and nano-plastics in a maritime, estuary or river environment includes multiple detection devices and a data processing device. The detection devices detect the plastics, macro-plastics, micro-plastics, and nano-plastics using different detection wavelengths. The data processing device includes a communication interface and a processor. The communication interface receives detection signals from the detection devices and the processor determines locations of the plastics, the macro-plastics, the micro-plastics and the nano-plastics in the environment based at least in part on the received detection signals within a common coordinate system.

Methods of studying characteristics of a sample over time are disclosed. The method includes irradiating a sample containing particles with a light beam to generate scattered light by interaction of the light beam with the sample, obtaining measurements of the scattered light over a measurement period divided into a plurality of shorter sub-processes, classifying each sub-process, and determining sample particle characteristics for the sub-processes. The method may also include transforming each sub-process prior to classifying each sub-process. Determining sample particle characteristics for the sub-processes may be performed prior to classifying each sub-process. The method may also include determining a change in the sample particle characteristic over time in the sub-process.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

The present invention provides a method for quantifying moving heterologous nanoparticles in a suspension by imaging. The quantifying method comprises acquiring at least one z-stack of images within the suspension; tracking the nanoparticles in the images to identify unique nanoparticles; and enumerating the unique nanoparticles. Also provided is a method for characterizing size distribution of moving heterologous nanoparticles in a suspension by imaging. The characterization method comprises acquiring time lapse images; tracking the nanoparticles in the images to identify unique nanoparticles; determining the locations of each of the unique particles; determining the size of each of the unique nanoparticles; and aggregating the sizes of the unique nanoparticles.

A condensation particle counter includes: a saturator formed therein with a first flow path for supplying vapor to air introduced from an outside; a condenser formed therein with a second flow path in which the air and the vapor introduced from the first flow path are condensed; and a detector for detecting droplets condensed in the condenser, wherein the second flow path is provided with a guiding portion for guiding condensate flowing downward along an inner surface of the second flow path to flow into the saturator.

The present invention discloses an automatic detection method for nanoparticles based on deep learning and AFM images. The method includes: collecting the original AFM images of nanoparticles and processing them to obtain a normalized height map; establishing a deep learning model and training the model; collecting the original AFM detection images of the nanoparticles to be detected and processing them; inputting the detected normalized height map into the trained model, and outputting the regions, aggregation information, and confidence information of the nanoparticles to be displayed in the detected visualization height map; obtaining the pixel-size factor, size information, size distribution information, and aggregation ratio of the nanoparticles according to the detected original images and detected height maps, and finally realizing the automatic detection of nanoparticles. The present invention can automatically segment the nanoparticles in the AFM images and further analyze the nanoparticle information, solving the problems of low accuracy, weak applicability, and semi-automation existing in the current nanoparticle analysis based on traditional machine vision methods.

The invention belongs to the technical field of photoelectric detection, and particularly relates to a micro-nano particle characterization system and characterization method based on an alternating current photovoltaic effect. The two technical problems in the aspects of sensitivity and measurement precision of the existing photoelectric detection technology are solved; the sensitivity of the photoelectric detector is remarkably improved; the sensitivity bottleneck of the traditional technology is broken through; the interference of continuous laser irradiation on the micro-nano particles is effectively reduced, and the measurement precision is improved. The technical breakthroughs not only widen the application range of the photoelectric detection technology, but also provide more accurate and reliable technical means for micro-nano particle characterization, and have important scientific significance and application value.

The invention relates to a method for determining the florescence of antibodies on exosomes using quality control of the measurement using two different control methods.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sensea fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Apparatuses and corresponding methods are provided for determining molecular properties of a molecule. To determine molecular properties of the molecule, a molecule library comprising the molecule is generated, a focused library comprising the molecule is generated by filtering the molecule library; the focused library is attached to a flow cell of the imaging instrument; and a fluid control module of the imaging instrument is controlled to incubate the flow cell with a fluid comprising labelled target molecules. A light source module and an imaging module of the imaging instrument are operated in a selected operational mode such that the light source module illuminates the flow cell and the imaging module captures light generated by fluorescence by the labelled target molecules within the flow cell. Image data is generated based on the captured light and the imaging data is analyzed to determine molecular properties of the at least one molecule.

A particle measuring device includes a flow cell unit including a cell hollow portion in which a liquid sample flows, a mount unit including an accommodation hole accommodating at least a portion of the flow cell unit, and a resonance unit positioned behind the mount unit and coupled to the mount unit. The mount unit includes a mount body in which the accommodation hole is formed, and a passage hole depressed in an outer surface of the mount body and communicating with the accommodation hole. The resonance unit includes a resonance body behind the mount body and coupled to the mount body, and a cavity that is a space formed in the resonance body and communicates with the passage hole.

A flow nanocytometer for measuring extracellular vesicles (EVs) may include a flow cell for receiving a core stream carry at least one EV, at least one excitation source module for outputting a light beam to interrogate a portion of the core stream, a detection module for detecting the light beam, and a processor communicatively coupled to a memory, the at least one excitation source, and the detection module. The processor may execute a method including receiving a plurality of electronic signals from the detection module; detecting an intensity of a polycystic kidney disease (PKD) associated protein expressed by the EVs based on the plurality of electronic signals; determining a quantity of the EVs based on the plurality of electronic signals; and determining a severity or a prognosis of polycystic kidney disease in the subject, based on the intensity of the PKD associated protein and the quantity of the EVs.

A device (100) for characterizing particles using nanoparticle tracking analysis is provided. The device comprises: a sample cell (101) for containing a sample, said sample comprising a plurality of particles suspended in a fluid; a light source (102) configured to illuminate the sample; an imaging system (103) configured to collect light (112) scattered or fluoresced by particles moving within the sample cell (101) and within a detection zone (111) of the imaging system (103) and to capture a video of particles moving within the detection zone (111); and a computer (104). The computer is configured to process the video to automatically determine the depth of the detection zone (111). Determining the depth of the detection zone (111) comprises: tracking particles moving within the detection zone (111), thereby generating a trajectory for each particle; and analyzing how the measured attribute of the trajectory within the sub-segment of the video varies as the size of the sub-segment varies.

Methods and systems for analyzing lipid nanoparticles using size exclusion chromatograph coupled with multi angle light scattering are disclosed.

A method for determining a characteristic of a flowing fluid having particles in a sample space by Fourier domain optical coherence tomography includes estimating a velocity of the fluid in the sample space; controlling an optical scanner to radiate a beam of light along an optical path to the fluid in the sample space and to sense a signal of interference of measurement light scattered back along the optical path mixed with reference reflected light, while moving the optical scanner, where the beam of light is moved with a scanner velocity being aligned with a velocity component of the velocity of the fluid perpendicular to the optical axis of the beam of light, processing the signal into a corresponding complex-valued optical path length resolved OCT signal, where the OCT signal represents the fluid in the sample space; determining the characteristic of the fluid based on the OCT signal.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

A device (10) for characterizing particles using nanoparticle tracking analysis (NTA) is provided. The device (10) comprises: a flow cell (101) for containing a sample comprising a plurality of particles suspended in a fluid; a pump (102) configured to provide a flow of the sample through the flow cell (101); a light source (103) configured to illuminate the sample; an imaging system (104) configured to collect light scattered or fluoresced by particles moving within the flow cell (101) and within a detection zone of the imaging system (102) and capture a video of the particles moving within the detection zone; and a computer (105) configured to process the video. The computer (105) is configured to determine an estimated flow rate of the sample through the flow cell (101). Determining the estimated flow rate includes performing one-dimensional particle tracking in a direction perpendicular to the expected flow direction of the sample.

The invention relates to a device (100) for locating an individual particle (2) in a sample (3) having a plurality of regions (31), the particle being capable of emitting photons when it receives light, the device comprising: - a sample holder (110); - a light source arranged to produce an illumination field suitable for illuminating the sample placed on its holder, an intensity of the illumination field having a temporal and spatial variation such that the intensity has, in each region of the sample, a predetermined temporal pattern different from the temporal patterns of the intensity in the other regions; - a detector (140) capable of converting photons emitted by the particle in response to the illumination thereof by the illumination field into a signal representative of the intensity of the illumination field received by the particle; - a computer (150) programmed to determine, on the basis of a temporal change in the signal derived from the detector and the temporal and spatial variation in the intensity of the illumination field, the region of the sample in which the particle is located.

A spectroscopic bioagent detection apparatus and method are provided. In one aspect, an optical detection system and method are used to identify and / or detect a virus or bacteria by using a microscope and measuring vibrational motion or phonons of the virus or bacteria. A further aspect of the present apparatus and method include automatically optically measuring vibrational motion or phonons of a target virus or bacteria, substantially in real-time, in vivo or in situ, automatically filtering out undesired background and living cell signals, and automatically identifying a characteristic of the virus or bacteria.

The invention describes a method for detecting biological agents suspended in the air in real time, comprising: emitting, towards an air sample, a beam of monochromatic polarized light whose wavelength corresponds to an absorption maximum of a biological molecule; receiving, at different scattering angles, the scattered light beam after passing through the sample; determining that there are particles in the sample that contain the biological molecules of interest if the intensity of the scattered light has a substantially higher peak than the rest of the scattered light; estimating the amount of said biological molecules according to the amplitude of said peak; and estimating the size of the particles containing the biological molecules as a function of the scattering angle where said peak was detected. The invention also describes a system for carrying out said procedure.

A temperature verification device for checking the accuracy of a temperature sensor of a dynamic light scattering instrument, the temperature verification device comprising: a body configured to be received in a sample cell holder; and the calibration temperature sensor is positioned in the main body at a position corresponding to the scattering volume of the dynamic light scattering instrument.

The invention belongs to the technical field of analysis of single particles, and particularly discloses a method for detecting the atomic number ratio of multiple elements in a single particle in a nano material, which can synchronously collect ion signals of all the elements in the single particle, accurately measure the intensity of each element in a transient signal, and improve the detection accuracy. A novel data processing method, namely a statistical distribution fitting method, is adopted, so that the real and accurate multi-element atomic ratio is calculated, and the inherent defects of the traditional SP-ICP-MS and TEM technologies are overcome; meanwhile, the problem that part of nano-particles are unstable in a water phase is solved through an organic phase oxygen adding and sampling method.

A method for sample analysis comprises loading a plurality of samples into a plurality of sample storage vessels of a liquid chromatography instrument; receiving a plurality of parameters corresponding to the plurality of samples, the sample storage vessels holding samples of the plurality of samples having different parameters; dynamically displaying results from a measurement operation by an electrophoretic light scattering measurement instrument of the samples having the different parameters; and contemporaneously performing a combination of sample collection operations, sample injection operations, and sample storage unit washing operations.

Methods and systems for analyzing lipid nanoparticles using size exclusion chromatography in combination with multi-angle light scattering are disclosed.

Systems and methods for analyzing fluid samples containing nanoparticles for the determination of nanoparticle count and nanoparticle elemental composition are described. In an aspect, a system embodiment includes, but is not limited to, a remote sampling system configured to receive a fluid sample containing nanoparticles, the remote sampling system including a particle counter configured to determine a number of nanoparticles present in the fluid sample via light scattering detection; a sample analysis system configured to receive the fluid sample from the remote sampling system, the sample analysis system including a mass spectrometer configured to determine an elemental composition of the nanoparticles present in the fluid sample; and a system controller communicatively configured to provide a distribution of the nanoparticles in the fluid sample by specific element type based on an output from each of the mass spectrometer and the particle counter.

Provided is a portable biosensor that includes a sample filter cartridge, a filter collector, an optical sphere, an electromagnetic radiation emitter, a photo-detector, a processor, a signal display, a vacuum pump, and a power supply. The sample filter cartridge selectively removes small molecules to minimize spectral interference in the detection signal. The sample is concentrated onto the filter collector and subjected to illumination by the electromagnetic radiation emitter, producing Raman-scattering. The optical sphere collects and distributes the Raman-scattering shifts, which then pass through a spectral filter to produce spectral filtered scattering, which is then reflected by the concave holographic flat-field grating onto the photo-detector. The data is displayed graphically to provide the Raman-scattering shift data. The data is compared with a database for sample identification. The device is contained within a housing that is small enough to be easily transported for field use.

High throughput single molecule tracking (htSMT) systems and methods are described in which the htSMT workflow is adapted to characterize the contribution of known and new pathways to interaction networks in living cells, such as protein signaling interaction networks.

In semiconductor manufacture requiring ultra high purity water, particles of specified sizes are detected using acoustic particle detection capable of detecting nanoparticles. A sensor unit receives a flow of water, with the pressure and rate of flow to the sensor controlled closely. In each sensor unit is a measurement cell to receive a continuous flow of water, with a transducer module engaged with the cell to deliver an acoustic pulse at a known level of intensity as the liquid flows through the cells, the acoustic pulse acting on the liquid at a focus zone to cause cavitation in the event a particle of size detectable at that intensity is present. A waveform emitter / receiver connected to the sensor unit sends high-frequency, high-voltage signal to the transducer module to cause the acoustic pulse, and it also receives a waveform echo indicating a cavitation event. The system is controlled by a microprocessor.

The optical interrogation device can have a substrate; an insulating layer supported by the substrate; a plasmonic layer supported by the substrate via the insulating layer; a cavity extending across both the plasmonic layer and the insulating layer to a bottom adjacent the substrate, the cavity being sized to receive a particle; and a layer of 2D material covering the substrate and defining the bottom of the cavity. The process of optically interrogating the particle can include the layer of 2D material attracting the particle into the cavity across the upper end, and, while the particle is in the cavity, acquiring an optical signal including a spectral signature of the particle.

Embodiments relate to a biosensor. The biosensor includes an electrochemical system having a generator electrode, a collector electrode, and electrolyte. The generator electrode includes a particle capture region having an aspect ratio that is greater than 1. The particle capture region is a portion of the generator electrode configured to attract a particle supported within the electrolyte. The electrochemical system is operated so as to bias one of the generator electrode or the collector electrode below its formal potential while biasing the other electrode above its formal potential. The biosensor includes a processing module configured to measure electric current generated by the electrochemical system.