405results about "Nanoparticle analysis" patented technology

A system for detecting plastics, macro-plastics, micro-plastics and nano-plastics in a maritime, estuary or river environment includes multiple detection devices and a data processing device. The detection devices detect the plastics, macro-plastics, micro-plastics, and nano-plastics using different detection wavelengths. The data processing device includes a communication interface and a processor. The communication interface receives detection signals from the detection devices and the processor determines locations of the plastics, the macro-plastics, the micro-plastics and the nano-plastics in the environment based at least in part on the received detection signals within a common coordinate system.

A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

A target may be tagged, and its position tracked to maintain the position of the target at the center (e.g., zero region) of a suppression beam, where suppression is minimal, so the signal from the target is not suppressed relative to signal from background molecules. Point scanning is not needed, as a pixelated detector of a camera can be used to acquire the position of the target in one shot. Images of the object from the pixelated detector can be analyzed to acquire the position of the target. To maintain the target in the zero, the beams may be steered onto the target. Alternatively or additionally, the sample may be moved to place the sample in the center. A correction signal may be sent to cause the sample and / or beams to be moved to maintain the target in position.

Methods of studying characteristics of a sample over time are disclosed. The method includes irradiating a sample containing particles with a light beam to generate scattered light by interaction of the light beam with the sample, obtaining measurements of the scattered light over a measurement period divided into a plurality of shorter sub-processes, classifying each sub-process, and determining sample particle characteristics for the sub-processes. The method may also include transforming each sub-process prior to classifying each sub-process. Determining sample particle characteristics for the sub-processes may be performed prior to classifying each sub-process. The method may also include determining a change in the sample particle characteristic over time in the sub-process.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

The present invention provides a method for quantifying moving heterologous nanoparticles in a suspension by imaging. The quantifying method comprises acquiring at least one z-stack of images within the suspension; tracking the nanoparticles in the images to identify unique nanoparticles; and enumerating the unique nanoparticles. Also provided is a method for characterizing size distribution of moving heterologous nanoparticles in a suspension by imaging. The characterization method comprises acquiring time lapse images; tracking the nanoparticles in the images to identify unique nanoparticles; determining the locations of each of the unique particles; determining the size of each of the unique nanoparticles; and aggregating the sizes of the unique nanoparticles.

A condensation particle counter includes: a saturator formed therein with a first flow path for supplying vapor to air introduced from an outside; a condenser formed therein with a second flow path in which the air and the vapor introduced from the first flow path are condensed; and a detector for detecting droplets condensed in the condenser, wherein the second flow path is provided with a guiding portion for guiding condensate flowing downward along an inner surface of the second flow path to flow into the saturator.

The present invention discloses an automatic detection method for nanoparticles based on deep learning and AFM images. The method includes: collecting the original AFM images of nanoparticles and processing them to obtain a normalized height map; establishing a deep learning model and training the model; collecting the original AFM detection images of the nanoparticles to be detected and processing them; inputting the detected normalized height map into the trained model, and outputting the regions, aggregation information, and confidence information of the nanoparticles to be displayed in the detected visualization height map; obtaining the pixel-size factor, size information, size distribution information, and aggregation ratio of the nanoparticles according to the detected original images and detected height maps, and finally realizing the automatic detection of nanoparticles. The present invention can automatically segment the nanoparticles in the AFM images and further analyze the nanoparticle information, solving the problems of low accuracy, weak applicability, and semi-automation existing in the current nanoparticle analysis based on traditional machine vision methods.

A device (200) for collecting and analyzing aerosol particles includes a fluid storage tank (206) for generating a condensing fluid (256) having a first temperature; and an aerosol collection port (202) having a vapor inlet (212) for receiving the condensing fluid, and an aerosol inlet (222) for receiving an aerosol stream having a second temperature that is lower than the first temperature. The condensing fluid (256) is mixed with the aerosol stream in the aerosol collection port (202) producing a mixed stream. The device also includes a growth tube (204) configured to receive the mixed stream and condense aerosol particles to form a gaseous stream containing grown droplets which contain aerosol particles; and a converging nozzle (248) in fluid communication with the growth tube (204). The device allows for semi-continuous measurement of aerosol samples, and the measurement cycle can include: i) collection of particles onto a substrate (238), ii) analysis using spectroscopic techniques, and iii) removal of samples and preparing for next measurement.

The invention belongs to the technical field of photoelectric detection, and particularly relates to a micro-nano particle characterization system and characterization method based on an alternating current photovoltaic effect. The two technical problems in the aspects of sensitivity and measurement precision of the existing photoelectric detection technology are solved; the sensitivity of the photoelectric detector is remarkably improved; the sensitivity bottleneck of the traditional technology is broken through; the interference of continuous laser irradiation on the micro-nano particles is effectively reduced, and the measurement precision is improved. The technical breakthroughs not only widen the application range of the photoelectric detection technology, but also provide more accurate and reliable technical means for micro-nano particle characterization, and have important scientific significance and application value.

The invention relates to a method for determining the florescence of antibodies on exosomes using quality control of the measurement using two different control methods.

The present application relates to a device for measuring micro-nano particles, and the related use thereof. The device comprises: a first chamber having a first electrode; a second chamber adjacent to the first chamber and having a second electrode; a substrate that separates the first chamber from the second chamber; a membrane covering the side of the substrate close to the first chamber, wherein the membrane has a micropore; the substrate has an electro-osmotic cell with an inner diameter larger than the pore diameter of the micropore and smaller than the inner diameter of the second chamber; the first chamber is in communication with the second chamber by means of the micropore and the electro-osmotic cell; inner walls of the electro-osmotic cell are provided with electro-osmotic cell modification layers, which have a specific Zeta potential at an interface when coming into contact with an electrolyte.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sensea fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Apparatuses and corresponding methods are provided for determining molecular properties of a molecule. To determine molecular properties of the molecule, a molecule library comprising the molecule is generated, a focused library comprising the molecule is generated by filtering the molecule library; the focused library is attached to a flow cell of the imaging instrument; and a fluid control module of the imaging instrument is controlled to incubate the flow cell with a fluid comprising labelled target molecules. A light source module and an imaging module of the imaging instrument are operated in a selected operational mode such that the light source module illuminates the flow cell and the imaging module captures light generated by fluorescence by the labelled target molecules within the flow cell. Image data is generated based on the captured light and the imaging data is analyzed to determine molecular properties of the at least one molecule.

A particle measuring device includes a flow cell unit including a cell hollow portion in which a liquid sample flows, a mount unit including an accommodation hole accommodating at least a portion of the flow cell unit, and a resonance unit positioned behind the mount unit and coupled to the mount unit. The mount unit includes a mount body in which the accommodation hole is formed, and a passage hole depressed in an outer surface of the mount body and communicating with the accommodation hole. The resonance unit includes a resonance body behind the mount body and coupled to the mount body, and a cavity that is a space formed in the resonance body and communicates with the passage hole.

A flow nanocytometer for measuring extracellular vesicles (EVs) may include a flow cell for receiving a core stream carry at least one EV, at least one excitation source module for outputting a light beam to interrogate a portion of the core stream, a detection module for detecting the light beam, and a processor communicatively coupled to a memory, the at least one excitation source, and the detection module. The processor may execute a method including receiving a plurality of electronic signals from the detection module; detecting an intensity of a polycystic kidney disease (PKD) associated protein expressed by the EVs based on the plurality of electronic signals; determining a quantity of the EVs based on the plurality of electronic signals; and determining a severity or a prognosis of polycystic kidney disease in the subject, based on the intensity of the PKD associated protein and the quantity of the EVs.

The invention concerns a single-particle inductively-coupled plasma (ICP) mass spectrometry particle sizing and counting method comprising providing or receiving an intensity-versus-counts histogram of particles detected using an ICP mass spectrometer, the intensity representing particle detection and the count representing particle detection frequency; providing or receiving mass flux calibration data or calibration curve data relating a value of the intensity measurement or data of the ICP mass spectrometer to a mass of material detected per acquisition interval or dwell time; determining a particle mass of the particles detected using the mass flux calibration data or the at least one mass flux calibration curve data, determining a particle volume of the detected particles using the determined particle mass, and determining a particle size of the particles detected using the determined particle volume of the particles detected and a determined or attributed geometry or shape of the detected particles.

A device (100) for characterizing particles using nanoparticle tracking analysis is provided. The device comprises: a sample cell (101) for containing a sample, said sample comprising a plurality of particles suspended in a fluid; a light source (102) configured to illuminate the sample; an imaging system (103) configured to collect light (112) scattered or fluoresced by particles moving within the sample cell (101) and within a detection zone (111) of the imaging system (103) and to capture a video of particles moving within the detection zone (111); and a computer (104). The computer is configured to process the video to automatically determine the depth of the detection zone (111). Determining the depth of the detection zone (111) comprises: tracking particles moving within the detection zone (111), thereby generating a trajectory for each particle; and analyzing how the measured attribute of the trajectory within the sub-segment of the video varies as the size of the sub-segment varies.

The embodiment of the present application discloses a device and method for measuring micro-nano particles, the device comprising: a first chamber, a microporous membrane, an electrophoresis tank, and a second chamber, wherein the microporous membrane is located between the first chamber and the electrophoresis tank, and the microporous membrane has micropores, and the first chamber is connected to the electrophoresis tank through the micropores; the second chamber is adjacent to the electrophoresis tank, and has a first side wall located between the second chamber and the electrophoresis tank, and the first side wall has a first opening for connecting the second chamber and the electrophoresis tank; the inner diameter of the micropore is smaller than the inner diameter of the electrophoresis tank, and the inner diameter of the electrophoresis tank is smaller than the inner diameter of the second chamber; the first chamber has a first electrode, and the second chamber has a second electrode. The technical solution of the embodiment of the present application can measure the properties of a single micro-nano particle in a solution.

This disclosure provides a microfluidic system (e.g., microfluidic cartridge, microfluidic chip) comprising two or more microfluidic flow channels for impedance-based detection of a biological entity in a sample. The disclosed system enables simultaneous measurements of a sample in two or more microfluidic flow channels to minimize faulty results. It eliminates the need of lysing samples and measuring them multiple times that is more time-consuming, and could introduce some variations across samples and devices.

Methods and systems for analyzing lipid nanoparticles using size exclusion chromatograph coupled with multi angle light scattering are disclosed.

A method for determining a characteristic of a flowing fluid having particles in a sample space by Fourier domain optical coherence tomography includes estimating a velocity of the fluid in the sample space; controlling an optical scanner to radiate a beam of light along an optical path to the fluid in the sample space and to sense a signal of interference of measurement light scattered back along the optical path mixed with reference reflected light, while moving the optical scanner, where the beam of light is moved with a scanner velocity being aligned with a velocity component of the velocity of the fluid perpendicular to the optical axis of the beam of light, processing the signal into a corresponding complex-valued optical path length resolved OCT signal, where the OCT signal represents the fluid in the sample space; determining the characteristic of the fluid based on the OCT signal.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

A device (10) for characterizing particles using nanoparticle tracking analysis (NTA) is provided. The device (10) comprises: a flow cell (101) for containing a sample comprising a plurality of particles suspended in a fluid; a pump (102) configured to provide a flow of the sample through the flow cell (101); a light source (103) configured to illuminate the sample; an imaging system (104) configured to collect light scattered or fluoresced by particles moving within the flow cell (101) and within a detection zone of the imaging system (102) and capture a video of the particles moving within the detection zone; and a computer (105) configured to process the video. The computer (105) is configured to determine an estimated flow rate of the sample through the flow cell (101). Determining the estimated flow rate includes performing one-dimensional particle tracking in a direction perpendicular to the expected flow direction of the sample.

A critical lighting device is provided. A critical illumination device includes uniform illumination of an object in a reflected light microscope, the critical illumination device including: a coherent or partially coherent light source; an optical fiber coupled to the light source, wherein the optical fiber includes a core having a diameter of at least 15 microns and a numerical aperture of 0.1 to 0.5; and at least one collimating lens located at a focal distance from the tip of the optical fiber, where the collimating lens is configured to match a numerical aperture of the optical fiber to couple light out of the optical fiber.

The invention relates to a device (100) for locating an individual particle (2) in a sample (3) having a plurality of regions (31), the particle being capable of emitting photons when it receives light, the device comprising: - a sample holder (110); - a light source arranged to produce an illumination field suitable for illuminating the sample placed on its holder, an intensity of the illumination field having a temporal and spatial variation such that the intensity has, in each region of the sample, a predetermined temporal pattern different from the temporal patterns of the intensity in the other regions; - a detector (140) capable of converting photons emitted by the particle in response to the illumination thereof by the illumination field into a signal representative of the intensity of the illumination field received by the particle; - a computer (150) programmed to determine, on the basis of a temporal change in the signal derived from the detector and the temporal and spatial variation in the intensity of the illumination field, the region of the sample in which the particle is located.

The invention discloses a method of distinguishing empty and full capsids in a virus preparation or loaded and non-loaded non-viral gene therapy vectors. The method comprises the steps of: a) providing a preparation of viral particles or gene therapy vectors; b) subjecting the preparation to interferometric scattering mass spectrometry (ISCAMS), in an interferometric scattering microscope, to generate mass distribution data for the viral particles; c) determining the levels of empty capsids and capsids comprising a genome among the viral particles or the loaded and non-loaded vectors from the mass distribution data.

A spectroscopic bioagent detection apparatus and method are provided. In one aspect, an optical detection system and method are used to identify and / or detect a virus or bacteria by using a microscope and measuring vibrational motion or phonons of the virus or bacteria. A further aspect of the present apparatus and method include automatically optically measuring vibrational motion or phonons of a target virus or bacteria, substantially in real-time, in vivo or in situ, automatically filtering out undesired background and living cell signals, and automatically identifying a characteristic of the virus or bacteria.

The present invention relates to the field of performance measurement of nano-micron particles, and in particular to a method for jointly measuring the specific surface area and surface charge density of nano-micron charged particles: preparing H + Saturated sample; the anions corresponding to the two indicator ions are selected as Cl ‑ , A and B are K + 、Na + , Ca 2+ any two ions in the equilibrium system; determine the activity of the indicator ion in the equilibrium system; calculate the concentration of the indicator ion in the equilibrium system; calculate the adsorption amount of the indicator ion on the surface of the nano-micron particle: calculate the surface potential of the nano-micron particle; calculate the surface charge density of the particle; obtain the specific surface area of ​​the particle. When the ion exchange on the particle surface reaches equilibrium, the present invention only needs to determine the concentration of the two indicator ions in the equilibrium solution to directly calculate the specific surface area and surface charge density of the particle.

The invention describes a method for detecting biological agents suspended in the air in real time, comprising: emitting, towards an air sample, a beam of monochromatic polarized light whose wavelength corresponds to an absorption maximum of a biological molecule; receiving, at different scattering angles, the scattered light beam after passing through the sample; determining that there are particles in the sample that contain the biological molecules of interest if the intensity of the scattered light has a substantially higher peak than the rest of the scattered light; estimating the amount of said biological molecules according to the amplitude of said peak; and estimating the size of the particles containing the biological molecules as a function of the scattering angle where said peak was detected. The invention also describes a system for carrying out said procedure.