Sorter and sorting method for separating cells and particles

A particle separation and sorting technology, applied in the biological field, can solve the problems of not being able to expand the single-cell level, not being able to sort cells/particles, etc.

Active Publication Date: 2011-04-20
长春长光辰英生物科学仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention aims at the existing bottleneck problem of synthetic biology research, that is, it is impossible to sort nano-micron cells / particles at the single-cell level, so that modern synthetic biology ideas and systems biology methods cannot be extended to the single-cell level And the deficiencies at the level of non-cultivable microbial systems, providing a sorter and a sorting method for the separation of nano-microscale cells and particles

Method used

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  • Sorter and sorting method for separating cells and particles
  • Sorter and sorting method for separating cells and particles

Examples

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Embodiment 1

[0066] Example 1 Construction of Raman Flow Sorter for Cell and Particle Separation

[0067] 1) Raman spectrum acquisition and analysis system

[0068] The Raman flow sorting instrument used for the separation of cells and particles constructed in this example, the Raman spectrometer in the Raman spectrum acquisition and analysis system uses a confocal micro Raman spectrometer (PrincetonInstrument, US), and the excitation wavelength is 514.5nm (Coherent, Innova 90-5 Ar-ion). A Leica DM-IRB microscope with a 63x objective lens (Leica, HCXPL APO) was used for nano-microscale microscopy. Raman spectrum signal acquisition and analysis software uses Labs pec 5.0.

[0069] 2) Microfluidic control system

[0070] In the Raman flow sorter microfluidic control system for cell and particle separation constructed in this example, the inner diameter of the sample injection channel of the microfluidic device is 100 μm, and the length is 20 mm; the inner diameter of the protective liquid...

Embodiment 2

[0073] Example 2 Sorting of isotope-labeled cells by a Raman flow sorter for cell and particle separation

[0074] (1) Prepare target cell / particle samples and prepare sorting protection solution

[0075] Use liquid mineral medium as cell culture medium, 1 liter of liquid mineral medium contains 2.5g Na 2 HPO 4 , 2.5g KH 2 PO 4 , 1.0g NH 4 Cl, 0.1g MgSO 4 ·7H 2 O, 10 μL saturated CaCl 2 solution, 10 μL saturated FeSO 4 solution and 1mL Bauchop & Elsden. Among them, 1L of Bauchop& Elsden solution contains 10.75g MgSO4, 4.5g FeSO 4 ·7H 2 O, 2.0g CaCO 3 , 1.44gZnSO 4 ·7H 2 O, 1.12g MnSO 4 4H 2 O, 0.25g CuSO 4 ·5H 2 O, 0.28g CoSO 4 ·7H 2 O, 0.06g H 3 BO 3 and 51.3 mL of saturated HCl solution.

[0076] Add 100 μL of seawater sample to 10 mL of liquid mineral medium, and add 1.70 mg of carbon-13-labeled NaHCO at the same time 3 As the only carbon source, its concentration is 2mM. Shake culture at 30°C for 14 days to obtain a cell solution to be sorted.

[0...

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Abstract

The invention relates to a sorter and a sorting method for separating cells and particles. The sorter comprises a Raman spectrum acquisition analysis system, a micro-flow control system and a cell / particle sorting system, wherein the micro-flow control system is connected with the Raman spectrum acquisition analysis system; the Raman spectrum acquisition analysis system is connected with the cell / particle sorting system; and the cell / particle sorting system is connected with the micro-flow control system. The invention also provides a sorting method for separating the cells and the particles.The sorter can effectively identify and sort nanometer-micron sized cells, nano materials, particles and cell contents, and because the Raman spectrum has the characteristics of high speed, non-invasive property, high information content and the like, the sorting method provides richer characteristic information of the cells or the particles in the same time period compared with methods of fluorescence or radio isotope labeling and the like.

Description

technical field [0001] The invention relates to a sorting apparatus and a sorting method for separating cells and particles, in particular to a sorting apparatus and a sorting method for separating nano-microscale cells and particles, and belongs to the field of biotechnology. Background technique [0002] Synthetic biology uses molecular biology methods to systematically modify cellular software (genetic material represented by DNA) and hardware (organelles represented by ribosomes) to have specific functions. These living cells can self-replicate and act as "synthetic factories" to convert simple and cheap energy and substances (such as sunlight and carbon dioxide) into high value-added medicines, materials and bioenergy, or to eliminate refractory pollutants . As the 2009 Royal Society of Engineering report put it, the breakthroughs and benefits brought by synthetic biology "will become an important part of national wealth." However, the development of synthetic biology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B07C5/342G01N21/65
CPCB01L2300/0816G01N2015/0038G01N2015/149B01L2200/0652B01L2400/0415B01L3/00B01L2300/0864B01L2400/043B01L3/502761G01N21/65B01L2200/0636G01N15/1459B01L2400/0487G01N1/38Y10T436/12
Inventor 曾雨黄巍李梦秋张大奕
Owner 长春长光辰英生物科学仪器有限公司
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