4121results about "Fluid controllers" patented technology

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.

A diagnostic test device to perform a test on a biological or environmental sample is provided. In one aspect, the diagnostic test device includes a chamber configured to receive a fluid from a sample preparation reservoir at a first section of the chamber, and a plurality of spaced apart valleys along an inner surface of the at least one chamber. Each of the plurality of spaced apart valleys include a curved cross-section and is configured to promote flow of the fluid toward a second section of the at least one chamber.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to a centrifugal micro-fluidic chip for automatic detection of a nano-enzyme test strip and application of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip integrates the steps of sample adding, chromatography, color development and the like of the nano-enzyme test strip, and full automation of nano-enzyme experiments on the micro-fluidic chip is realized. Comprising a reaction structure main body, a bottom runner layer, a vertical through hole layer, a pressing layer, a top runner layer and a sealing body. The closed structure of the chip effectively reduces the interference of external environmental factors on the experiment, reduces the risk of sample pollution, and ensures the high reliability of the detection result.

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

Technologies for thaw detection are described. The system includes a sensor included in a container, a sensor blocking mechanism, and a detector. The container includes a fluid. The sensor blocking mechanism prevents detection of the sensor while a portion of the fluid in the container is in a frozen state. The detector is configured to detect the sensor while the portion of the fluid in the container is in a thawed state. The thawed state deactivates the sensor blocking mechanism by activating a movement of the sensor.

This disclosure relates to apparatus and methods for molecular diagnostics. Certain embodiments include a piston cycled from a first position proximal to a first end of a housing, to a second position proximal to a second end of the housing, and back to the first position proximal to the first end of the housing. In some embodiments, the present disclosure relates to devices, methods, and systems for molecular diagnostics that do not comprise a piston.

To provide methods for selectively combining discrete entities including cells, reagents, drugs, hydrogels, extracellular matrices, beads, particles, biological materials, media, or a combination thereof.SOLUTION: The method comprises sorting a plurality of discrete entities and trapping two or more discrete entities for a time sufficient for the two or more discrete entities to combine to form a combined discrete entity. In certain aspects, the method comprises making the plurality of discrete entities. In certain aspects, the method comprises detecting or analyzing the discrete entities, for example, via optical detection. In certain aspects, the method comprises manipulating or analyzing the combined discrete entity or a component therein, for example, imaging, sequencing, culturing, for example, three-dimensional culturing, and measuring cell-cell interactions.SELECTED DRAWING: None

A temperature regulation system for samples in a microplate includes a housing defining an interior volume and first and second airflow ports defined by a first and second exterior surfaces of the housing. The system includes an electromagnetic mixing system that has a base plate defining a plurality of openings, and a plurality of electromagnets. Each electromagnet defines an axis that extends substantially vertically from the base plate. The base plate is disposed in the interior volume and each of the plurality of electromagnets extend toward the first airflow port. A fan is disposed in the interior volume substantially below the base plate. Activation of the fan draws air into the first or second airflow port, substantially parallel to each axis of the plurality of electromagnets, through the plurality of openings, and out of the other of the second and first airflow port.

The invention discloses a closed-loop control digital microfluidic system and a method thereof, and relates to the technical field of microfluidics, and the method comprises the following steps: initializing a digital microfluidic chip electrode array, setting an electrode spacing, an electrode size and a voltage upper limit, collecting a no-load state signal, and generating initial reference data; liquid drops are formed at the preset position of the digital micro-fluidic chip, and according to the initial reference data, the initial position, the volume and the form information of the liquid drops are collected, and a liquid drop initial state data set is generated; inputting the initial state data set of the liquid drop into a pre-trained closed-loop prediction model, performing kinetic analysis and trajectory prediction calculation, generating an expected motion trajectory of the liquid drop, and comparing the expected motion trajectory with the real-time state data flow of the liquid drop to generate deviation feedback data of the liquid drop; and dynamically adjusting the initial driving voltage parameter according to the droplet deviation feedback data to generate a closed-loop driving voltage sequence. The liquid drop trajectory control precision is improved, and motion errors caused by environmental disturbance and electrode response differences are reduced.

The invention discloses an exosome enrichment microfluidic device based on a synergistic effect. The exosome enrichment microfluidic device comprises a continuous phase injection device, a dispersed phase injection device, a laser, a microfluidic enrichment module, a continuous phase infusion tube, a dispersed phase infusion tube, a continuous phase variable-diameter connecting tube, a dispersed phase variable-diameter connecting tube and a liquid outlet tube. According to the device disclosed by the invention, through a synergistic driving mechanism of a thermophoresis effect, natural convection and thermal capillary convection, the exosome is directionally migrated and efficiently enriched on the surface of the dispersed phase liquid drop, the dispersed phase liquid drop is used as a targeted interface carrier for exosome enrichment, the exosome is stably adsorbed by utilizing interface force, and the enrichment specificity and efficiency are improved.

A method for processing a sample in a container includes introducing the sample to the container. A cap is applied to the container. The cap contains a plurality of beads. The plurality of beads is mixed with the sample. The plurality of beads is separated from the sample. Subsequent to removing the plurality of beads, a further process is initiated.

A system for automated extraction of biological samples from a set of biological samples has a small footprint and minimal movement of consumable sets, thereby reducing potential contamination during the extraction process. The extraction system includes a set of reaction tubes, a storage unit for storing a set of consumables, a sample preparation unit, a sample extraction unit, a waste unit, a plurality of robots for moving the tubes, samples, and cassettes, and a programmable control system programmed to process the samples in a serial mode in which a series of samples are processed sequentially in time one after another, thereby maintaining a continuous mode. Thus, a fixed processing turnaround time for each sample is maintained regardless of when the sample starts processing.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

The invention provides an automatic sample distribution method for flow cytometry, which belongs to the technical field of flow cytometry and comprises the following steps: acquiring basic information of a sample; the method comprises the following steps: pre-analyzing a sample through a micro-fluidic chip to obtain sample density, sample viscosity and sample ion concentration; measuring the micro-channel diameter and the micro-channel length of the micro-fluidic chip by adopting a laser ranging system, and recording the characteristic parameters of the micro-channel material; a target distribution volume is set, a high-precision injection pump system is started for pre-injection, and an initial distribution rate is obtained through measurement of a flow sensor; calculating the initial distribution rate by using a fluid dynamic compensation equation set to obtain a corrected distribution rate; controlling the high-precision injection pump system to perform sample distribution according to the corrected distribution rate; performing real-time monitoring on a sample liquid level by using an ultrasonic sensor, and synchronously acquiring a real-time distribution rate; and sample shunting is performed through the micro-fluidic chip, so that multi-channel parallel distribution is realized.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods (COGS) savings and reduced contamination levels.

Provided herein are systems and methods for collecting biological samples. The sample may be collected using a sample collection device, where the device is configured to prevent oxidation of the sample within the device. The sample collection device may include a component that absorbs oxygen within the device. The sample collection device may be used to preserve the sample for transport. The sample collection device may be used to preserve the sample for later analysis. The sample collection device may include a membrane with one or more test areas for detecting one or more target analytes within the sample.

Centrifuge (1) for cleaning a reaction vessel unit (7) with a rotor (2) and a rotor chamber (9) in which the rotor (2) is arranged and rotatably mounted, wherein the rotor (2) has a receiving area (6) for receiving the reaction vessel unit (7), and the rotor chamber (9) is delimited by a housing (3), wherein the housing (3) has an outlet for discharging liquid discharged from the reaction vessels and is provided with an inlet for supplying the rotor chamber (9) with a cleaning solution such that the cleaning solution is distributed in the rotor chamber (9) by rotation of the rotor (2) through contact with the rotor (2), so that the rotor chamber (9) is cleaned, wherein the rotation axis (5) of the rotor (2) runs parallel to a base (16) and wherein the centrifuge has a control device which is designed such that - the rotor chamber (9) is filled with a cleaning solution at least to a level (43) such that when the rotor (2) rotates, it is at least partially immersed in the cleaning solution, - the rotor (2) is rotated, whereby the cleaning solution is distributed in the rotor chamber (9), and - the cleaning solution is removed from the rotor chamber (9).

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention relates to the technical field of biological medicine industry, and provides a multi-stage vacuum heat-insulation high-temperature experimental facility which comprises a body, the body comprises an external box body and an inner container fixed in the box body, a flow guide cover is fixedly installed in the inner container, a detachable material plate is installed in the flow guide cover in a clamped mode, and the material plate is connected with the box body. Steps which are diagonally arranged are formed at the upper end and the lower end of the inner container, an air channel is formed between the inner container and the flow guide cover, an air cavity is formed between the inner side of each step and the flow guide cover, and a movable push plate is arranged in each air cavity; it can be understood that the flow guide cover is arranged in the inner container, the air channel and the air cavity are formed between the flow guide cover and the inner container, airflow circulation is driven by means of movement of the push plate in the air cavity, and materials are evenly heated on the material plate; meanwhile, the material plate is clamped in the flow guide cover through the elastic piece and can be easily installed and taken out through operation of a hand lever, direct contact with the hand is avoided, and using convenience and safety are improved.

The invention provides a liquid drop continuous generation device which comprises a first flow channel and a second flow channel, the first flow channel and the second flow channel comprise first positions which are tightly attached to each other, and each first position comprises a through hole communicating with the first flow channel and the second flow channel; the first flow channel and the second flow channel are used for introducing a first liquid and a second liquid respectively; the special ultrasonic device can act on the first liquid in the first flow channel, when the special ultrasonic device is driven in a first power period, an acoustic beam current facing the through hole is generated in the first liquid, the acoustic beam current enters the second liquid through the through hole, and liquid drops formed by the first liquid are formed in the second liquid. The invention also correspondingly provides a method for continuously generating liquid drops. The liquid drop generation mode is more flexible, the controllability is higher, and the cost is lower.

The invention belongs to the technical field of ultra-precision machining, and discloses a micro-fluidic chip based on the electroosmosis effect and a stress regulation and control method thereof.The micro-fluidic chip is internally provided with a plurality of solution channels, each solution channel is of a snakelike reciprocating structure, one end of each solution channel is provided with a channel inlet, and the other end of each solution channel is provided with a channel outlet; a plurality of inflection points arranged at intervals are arranged on one side, facing the bottom of the micro-fluidic chip, of the solution channel, the inflection points are close to the bottom of the micro-fluidic chip and are distributed on the micro-fluidic chip in the horizontal direction, and an electrode is further arranged on the solution channel. The electroosmosis effect and the microfluidic technology are combined, high-precision dynamic stress regulation and control are achieved through gradient flow channel design, a composite driving liquid formula and a hysteresis compensation algorithm, and the problems that pressure control response is delayed and precision is insufficient in a traditional polishing technology are solved; and a new technical approach is provided for ultra-precision machining of the large-aperture optical element for the inertial confinement nuclear fusion device.

The invention provides a device for sorting and enriching and application of the device, and the device comprises a shell; screening holes are formed in the sorting chips, the N sorting chips which are sequentially arranged divide the inner space of the shell into N + 1 cavities, and N is larger than or equal to 1; wherein a first cavity is formed between one side of the shell and the sorting chip adjacent to the shell; a second cavity is formed between the other side of the shell and the sorting chip adjacent to the shell; a first outlet and a first inlet which are used for connecting the first cavity with the outside are formed in the cavity wall of the first cavity; and a second outlet for connecting the second cavity with the outside is formed in the cavity wall of the second cavity.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

A method of culturing cells, the method including providing a microfluidic device including a substrate including an array of grooves that are substantially parallel; introducing cells into one or more of the grooves; providing a flow of culture medium over the groove array in a first flow direction that is across the grooves; culturing the cells under conditions to support viability, growth or differentiation, optionally wherein: (i) the cells are introduced into one or more grooves by flow over the groove array in the first flow direction; and / or (ii) the flow of culture medium is provided at a flow rate equivalent to 0.2-5 mL per million cells per day; and / or (iii) the cells are cultured for 1-14 days.

A diagnostic test device to perform a test on a biological or environmental sample is provided. In one aspect, the diagnostic test device includes a chamber configured to receive a fluid from a sample preparation reservoir at a first section of the chamber, and one or more capillary grooves along an inner surface of the chamber. The capillary grooves are configured to promote flow of the fluid toward a second section of the chamber. Embodiments of the diagnostic test reservoir can increase an amount of sample available to an assay reaction, which in one example embodiment occurs within a volume of fluid in the second section of the chamber.

Provided herein are methods for replenishing dissolved gas to aqueous droplets on a digital microfluidic device. Provided herein are methods of cell-free protein synthesis, optimized cell-free protein synthesis (CFPS) reagents, and methods for optimizing CFPS reagents to increase protein expression yield. The method is suitable for protein expression on microfluidic devices having hydrophobic surfaces by circulating a fill fluid to introduce additional oxygen.