2682results about "Fluid controllers" patented technology

A diagnostic test device to perform a test on a biological or environmental sample is provided. In one aspect, the diagnostic test device includes a chamber configured to receive a fluid from a sample preparation reservoir at a first section of the chamber, and a plurality of spaced apart valleys along an inner surface of the at least one chamber. Each of the plurality of spaced apart valleys include a curved cross-section and is configured to promote flow of the fluid toward a second section of the at least one chamber.

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

The invention discloses a closed-loop control digital microfluidic system and a method thereof, and relates to the technical field of microfluidics, and the method comprises the following steps: initializing a digital microfluidic chip electrode array, setting an electrode spacing, an electrode size and a voltage upper limit, collecting a no-load state signal, and generating initial reference data; liquid drops are formed at the preset position of the digital micro-fluidic chip, and according to the initial reference data, the initial position, the volume and the form information of the liquid drops are collected, and a liquid drop initial state data set is generated; inputting the initial state data set of the liquid drop into a pre-trained closed-loop prediction model, performing kinetic analysis and trajectory prediction calculation, generating an expected motion trajectory of the liquid drop, and comparing the expected motion trajectory with the real-time state data flow of the liquid drop to generate deviation feedback data of the liquid drop; and dynamically adjusting the initial driving voltage parameter according to the droplet deviation feedback data to generate a closed-loop driving voltage sequence. The liquid drop trajectory control precision is improved, and motion errors caused by environmental disturbance and electrode response differences are reduced.

A system for automated extraction of biological samples from a set of biological samples has a small footprint and minimal movement of consumable sets, thereby reducing potential contamination during the extraction process. The extraction system includes a set of reaction tubes, a storage unit for storing a set of consumables, a sample preparation unit, a sample extraction unit, a waste unit, a plurality of robots for moving the tubes, samples, and cassettes, and a programmable control system programmed to process the samples in a serial mode in which a series of samples are processed sequentially in time one after another, thereby maintaining a continuous mode. Thus, a fixed processing turnaround time for each sample is maintained regardless of when the sample starts processing.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

This disclosure provides for devices, methods, and systems for generating a plurality of droplets within a collecting container at an extremely high rate (e.g., of at least 1 million droplets per minute, etc.), each of the plurality of droplets comprising an aqueous mixture for a digital analysis, wherein upon generation, the plurality of droplets is stabilized in position within a region of the collecting container. The inventions enable partitioning of samples for digital analyses at unprecedented rates, where readout of signals from targets within such partitions can still be achieved in accordance with various assays.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention relates to the technical field of biological medicine industry, and provides a multi-stage vacuum heat-insulation high-temperature experimental facility which comprises a body, the body comprises an external box body and an inner container fixed in the box body, a flow guide cover is fixedly installed in the inner container, a detachable material plate is installed in the flow guide cover in a clamped mode, and the material plate is connected with the box body. Steps which are diagonally arranged are formed at the upper end and the lower end of the inner container, an air channel is formed between the inner container and the flow guide cover, an air cavity is formed between the inner side of each step and the flow guide cover, and a movable push plate is arranged in each air cavity; it can be understood that the flow guide cover is arranged in the inner container, the air channel and the air cavity are formed between the flow guide cover and the inner container, airflow circulation is driven by means of movement of the push plate in the air cavity, and materials are evenly heated on the material plate; meanwhile, the material plate is clamped in the flow guide cover through the elastic piece and can be easily installed and taken out through operation of a hand lever, direct contact with the hand is avoided, and using convenience and safety are improved.

The invention belongs to the technical field of ultra-precision machining, and discloses a micro-fluidic chip based on the electroosmosis effect and a stress regulation and control method thereof.The micro-fluidic chip is internally provided with a plurality of solution channels, each solution channel is of a snakelike reciprocating structure, one end of each solution channel is provided with a channel inlet, and the other end of each solution channel is provided with a channel outlet; a plurality of inflection points arranged at intervals are arranged on one side, facing the bottom of the micro-fluidic chip, of the solution channel, the inflection points are close to the bottom of the micro-fluidic chip and are distributed on the micro-fluidic chip in the horizontal direction, and an electrode is further arranged on the solution channel. The electroosmosis effect and the microfluidic technology are combined, high-precision dynamic stress regulation and control are achieved through gradient flow channel design, a composite driving liquid formula and a hysteresis compensation algorithm, and the problems that pressure control response is delayed and precision is insufficient in a traditional polishing technology are solved; and a new technical approach is provided for ultra-precision machining of the large-aperture optical element for the inertial confinement nuclear fusion device.

The invention provides a device for sorting and enriching and application of the device, and the device comprises a shell; screening holes are formed in the sorting chips, the N sorting chips which are sequentially arranged divide the inner space of the shell into N + 1 cavities, and N is larger than or equal to 1; wherein a first cavity is formed between one side of the shell and the sorting chip adjacent to the shell; a second cavity is formed between the other side of the shell and the sorting chip adjacent to the shell; a first outlet and a first inlet which are used for connecting the first cavity with the outside are formed in the cavity wall of the first cavity; and a second outlet for connecting the second cavity with the outside is formed in the cavity wall of the second cavity.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

A diagnostic test device to perform a test on a biological or environmental sample is provided. In one aspect, the diagnostic test device includes a chamber configured to receive a fluid from a sample preparation reservoir at a first section of the chamber, and one or more capillary grooves along an inner surface of the chamber. The capillary grooves are configured to promote flow of the fluid toward a second section of the chamber. Embodiments of the diagnostic test reservoir can increase an amount of sample available to an assay reaction, which in one example embodiment occurs within a volume of fluid in the second section of the chamber.

The application relates to the chip technical field, in particular to a preparation method of a chip device and a film forming method. The preparation method comprises the following steps: providing a substrate layer, the substrate layer has a surface, and a plurality of micro-pore structures are formed on the surface, the micro-pore structures comprise: second layer micro-pores and first layer micro-pores; adding a first electrolyte into the micro-pore structures, the first electrolyte fills the first layer micro-pores; adding a film solution into the micro-pore structures, the film solution is polymerized and fills the gap between the first electrolyte and the first layer micro-pores; adding a viscous protective solution into the micro-pore structures, the viscous protective solution fills the second layer micro-pores, finally, the viscous protective solution, the film solution and the first electrolyte form a stable sandwich layer structure in the micro-pore structures. The amount of the first electrolyte, the film solution and the viscous protective solution is accurately controlled through centrifugation, so that the consistency of the finally formed film is ensured.

An example one-time-open microfluidic valve can include an inlet microfluidic channel in a first elevation plane and an outlet channel in a second elevation plane. An upstream end of the outlet channel can overlap a downstream end of the inlet microfluidic channel. A capillary nozzle opening can connect the inlet microfluidic channel to the outlet channel at the overlapping ends. The capillary nozzle opening can have an outlet width that is smaller than a width of the outlet channel. A fluid actuator can be positioned to eject a fluid in the inlet microfluidic channel through the capillary nozzle opening into the outlet channel.

A semiconductor biosensor diagnostic system is provided. The semiconductor biosensor diagnostic system includes a diagnostic cartridge and a cartridge diagnostic base. The diagnostic cartridge includes a biosensor device and a fluid-guide body over the biosensor device. The fluid-guide body includes a first pumping opening, a second pumping opening, a buffer reservoir, a sample reservoir, and a plurality of channels configured to provide a first loop including the first pumping opening and the buffer reservoir, and a second loop including the second pumping opening and the sample reservoir. The first loop and the second loop sharing a common channel passing through the biosensor device. The cartridge diagnostic base includes a plurality of micro pumps coupled to the first pumping opening and the second pumping opening, and a sensing structure configured to receive a sensing information from the biosensor device.

The invention discloses a system and a method for rapidly detecting trace active ingredients of a medicine, belongs to the technical field of medicine detection, and relates to cross application of surface enhanced Raman spectroscopy and artificial intelligence. The system comprises a sample preparation module, a micro-fluidic enrichment module, a Raman spectrum acquisition module, a self-adaptive spectrum analysis module, a multi-component identification module and an intelligent report generation module, and efficient enrichment and nanogram-level detection of drug molecules are realized through an enhanced surface Raman scattering micro-fluidic chip. Intelligent identification and accurate quantification of complex spectral characteristics are realized by adopting a deep learning algorithm and a self-adaptive weight calculation method, synchronous detection of 5-10 active pharmaceutical ingredients is supported, the detection sensitivity reaches nanogram / milliliter level, the quantification error is less than 5%, the detection time of the whole process is 5-10 minutes, and the detection accuracy is high. The sensitivity, the accuracy and the efficiency of medicine quality detection are obviously improved.

The invention provides a micro-fluidic chip system for fixing juvenile zebrafish with a matched injection pump facing upwards on the side / back surface as well as a preparation method and application of the micro-fluidic chip system. The micro-fluidic chip system for fixing the juvenile zebra fish with the matched injection pump facing upwards on the side / back surface comprises a conveying device, the juvenile zebra fish micro-fluidic chip and an analysis device, the conveying device comprises an injection pump and a conveying catheter. According to the side / back upward type micro-fluidic chip system for fixing the juvenile zebrafish by matching with the injection pump, and the preparation method and the application of the micro-fluidic chip system, provided by the invention, the juvenile zebrafish can be automatically fixed through automatic water flow injection under the condition of no anesthetic treatment by combining a fluid dynamic method with the shape of the juvenile zebrafish; meanwhile, the system has the advantages that high throughput can be realized, and potential technical support can be provided for further realizing drug screening, poison screening and toxicological research and related research of zebra fish.

The present invention relates to an apparatus for producing nanoparticles comprising a hydrophobic substance and a hydrophilic substance. Specifically, the apparatus according to the present invention is characterized in that it comprises: a plurality of inlet channels into which a hydrophobic substance and a hydrophilic substance are introduced, respectively; a mixing channel in which substances are mixed to produce nanoparticles; and an outlet channel through which the produced nanoparticles are discharged, in which the mixing channel includes a microcolumn capable of increasing the mixing efficiency of the substance. Accordingly, nanoparticles produced using the apparatus according to the present invention exhibit excellent particle uniformity and can be effectively used as a drug or a drug delivery carrier.

The invention discloses a high-pressure CO2 oil displacement micro-fluidic chip device which comprises an upper polar plate, a middle partition plate, a lower polar plate, a speed measurement module, a porous medium radial flow module and a liquid outlet module, the speed measurement module is arranged on the upper surface of the middle partition plate; the porous medium radial flow module and the liquid outlet module are arranged on the upper surface of the lower polar plate; the upper polar plate, the middle partition plate and the lower polar plate are sequentially stacked from top to bottom; the speed measurement module is connected with the porous medium radial flow module; and the porous medium radial flow module is connected with the liquid outlet module. According to the device disclosed by the invention, a pore throat structure in rock is simulated by constructing a micron-sized or even smaller complex flow channel, and the CO2 flooding crude oil flow characteristic and the CO2 distribution rule are simulated; a transparent visualization device structure is designed, and visualization of the action mechanism of the CO2 oil displacement system and the reservoir fluid distribution characteristics is guaranteed.

A portable centrifuge comprising a base, which includes a rotational mechanism contained within. A sequester wheel having a plurality of concentric rings, forming at least one channel and a central container. The at least one channel comprising a second volume and the central container comprising a first volume. A first cover is disposed over the sequester wheel to create a seal. A second cover is disposed over the first cover and is rotatable to engage with the rotational mechanism to initiate rotation at a predetermined RPM. The whole blood and / or other tissue is separated into its constituent components and deposited into the at least one channel and the central container. Specific volumes of the constituent components may be calculated to match or substantially match the first and second volumes for predetermined volume control and ease of access.

An analytical instrument having a syringe size identification functionality includes a syringe pump with syringe having size Vsyri, an electric drive, a valve downstream of the syringe, a pressure sensor between the syringe and the valve, a constant downstream volume Vc and a processor configured to control the electric drive to move the plunger from L0 for detecting pressure P1 to a pressure detection position Ld for detecting P2 using the pressure sensor. The processor is configured to calculate the theoretical pressure P2* using a modified version of Boyle's law: P2*=P1(Vsyri+Vc) / (Vsyri(1−x)+Vc) and compare the theoretical pressure P2* with the detected pressure P2. When the theoretical pressure P2* equals the detected pressure P2, the analytical system is released for use.

A system for assembling fields from a source substrate onto a second substrate. The source substrate includes fields. The system further includes a transfer chuck that is used to pick at least four of the fields from the source substrate in parallel to be transferred to the second substrate, where the relative positions of the at least four of the fields is predetermined.

Provided is an electrowetting-on-dielectric (EWOD) device. The EWOD device comprises a first substrate and a second substrate opposite to the first substrate. The first substrate comprises a. plurality of first electrodes configured to be respectively controllably connected to signal lines. The first hydrophobic layer disposed over the plurality of first electrodes. The second substrate comprises a second electrode configured to be connected to the signal lines. The second hydrophobic layer disposed over the second electrode. An internal space between the first substrate and the second substrate is provided for receiving a droplet and a surrounding medium. The droplet is surrounded by the surrounding medium.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and / or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

A method for introducing a liquid into a flow path device includes preparation, filling, first suction, and introduction. The flow path device includes a flow path portion inside to allow a liquid flow between first and second openings. A switcher includes first to third ports. The preparation includes connecting the flow path device and the switcher to allow a liquid flow between the second opening and the first port. The preparation includes connecting the second port and a liquid storage, connecting the third port and a suction-discharge unit, and connecting the first opening and a feeder. The filling includes filling, with a second liquid fed by the feeder, a flow path from the feeder to the liquid storage. The first suction includes sucking a first liquid using the suction-discharge unit from the liquid storage. The introduction includes introducing the first liquid into the flow path portion using the suction-discharge unit.

A channel chip includes a plurality of filter layers, a first common supply passage, a common channel, and a dielectrophoretic layer. The plurality of filter layers each have an HDF. The first common supply passage supplies a sample liquid to the HDFs of the plurality of filter layers. The liquid that has passed through the plurality of HDFs passes through the common channel. The dielectrophoretic layer includes electrodes for causing dielectrophoresis of dielectric particles contained in a liquid passing through the common channel. The plurality of filter layers and the dielectrophoretic layer are stacked.