27 results about "Microfluidic chamber" patented technology

Microfluidic assay devices that support multi-layer cellular matrices (e.g., ex-vivo skin cell matrices) and associated methods of fabrication and use are provided. A holder including a microfluidic chamber includes multiple sidewall ports disposed at different levels, multiple inlet ports in communication with different sidewall ports, and a liquid-permeable membrane bounding a chamber bottom. Stratified layers of cell-containing material may be formed in the chamber by sequential steps of introducing cell-hydrogel precursor material through a sidewall port and crosslinking the same, then repeating the steps for each sidewall port, to form cell-containing material layers in contact with one another. A cover with distribution channel(s) is provided below the liquid-permeable membrane. A continuous upper extension of the chamber may be open from above to provide interface(s) for receiving analytes and / or for environmental interaction. A holder may removably receive multiple assay devices.

The utility model provides a kind of F-P interference microfluid sensing detection device of full-suspension core optical fiber integration, including wideband light source and the coupler of signal connection, coupler is signal connected to No. one circulator and No. two circulators respectively, No. two circulators are also signal connected to No. two spectrometer and fan in fan out module respectively;No. one circulator is signal connected to No. one spectrometer and fan in fan out module respectively, the output end signal connection of fan in fan out module is used to pass through the F-P interference microfluid cavity structure of light beam and liquid to be measured, the F-P interference microfluid cavity structure is equipped with two liquid inlets and two liquid outlets, and each liquid inlet is communicated with a liquid outlet to form micropore channel, each liquid inlet is connected with a microfluid pump pipeline, and each liquid outlet is connected with a waste liquid pool pipeline.The utility model structure is highly integrated, can detect two kinds of liquid in parallel, improve work efficiency, the micropore channel of the setting of double-hole optical fiber, so that the amount of sample to be measured is very little to complete test.

The present invention relates to a microfluidic synthesis chip cartridge on which a synthesis chip including a microfluidic chamber formed in a predetermined shape is mounted, so that two or more fluids that do not mix well with each other are uniformly mixed to generate a synthesized sample. The purpose of the present invention is to provides a microfluidic synthesis chip cartridge wherein: the microfluidic synthesis chip cartridge includes a plate-shaped member on which two or more synthesis chips having microfluidic channels of a predetermined shape for mixing or reacting two or more liquid samples are mounted in parallel, thereby providing a structure and arrangement capable of dramatically increasing production per unit time despite the synthesis chips having microfluidic channels; and the raw-material supply timings of the two or more synthesis chips are aligned solely by changing the shapes of the microfluidic channels rather than by a separate electronically precise mechanism, thereby enabling synchronization among a plurality of synthesis chips with an inexpensive and simple structure.

The invention relates to a device for controlling the movement of a fluid (F) comprising: A microfluidic capsule (1) which comprises a microfluidic chamber (10), at least one microfluidic channel (11) opening into said microfluidic chamber (10), and a membrane (13) deformable inside said microfluidic chamber to move a volume of fluid outside the chamber towards said microfluidic channel or to suck a volume of fluid towards the inside of the microfluidic chamber, A device for actuating the membrane (13), the actuating device comprising an actuating channel (20) opening opposite the membrane,The actuating device comprising an actuating liquid (L) placed in the actuating channel (20) and pneumatic actuating means (2) connected to the actuating channel (20) and configured to inject an actuating gas (G) into said actuating channel (20) in order to pressurize said actuating liquid against the membrane (13) and deform it. Figure to be published with the abstract: Figure 2,

The invention relates to an analysis cartridge (1) for the detection and / or analysis of a species likely to be present in a liquid. The analysis cartridge (1) is provided with a front face and a rear face opposite the front face. The cartridge further comprises: - at least one microfluidic analysis chamber (5) for receiving the liquid, the at least one microfluidic chamber being formed within the cartridge and comprising a base (5a); - at least one first cluster (9) formed of magnetic nanoparticles held together and onto which capture agents are grafted, said capture agents being configured to bind specifically with the species, the at least one first cluster (9) being adherent to the base (5a). Figure 4b

This invention discloses a fiber optic microfluidic cavity MZI interferometric pH sensor and its fabrication method, belonging to the field of fiber optic biochemical sensing and biomedical detection technology. In an SMF-NCF-SMF fusion structure, an open microfluidic cavity is precisely etched within a coreless fiber segment using femtosecond laser processing technology, and a chitosan / polyacrylic acid pH-sensitive multilayer film is constructed on its inner wall using layer-by-layer self-assembly technology. This invention employs the aforementioned fiber optic microfluidic cavity MZI interferometric pH sensor and its fabrication method. By etching an open microfluidic cavity with a femtosecond laser within an SMF-NCF-SMF fusion structure and constructing a chitosan / polyacrylic acid pH-sensitive multilayer film on the cavity inner wall using layer-by-layer self-assembly technology, a synergistic effect of high sensitivity and low temperature crosstalk is achieved, while maintaining good mechanical stability and miniaturization characteristics.

A method of detecting passage of a particle into a target location includes receiving a sample on a die including a microfluidic chamber, the microfluidic chamber including a microfluidic path coupling a reservoir to a foyer, and moving the sample from the reservoir to the foyer by firing a nozzle fluidically coupled to the foyer. The method further includes detecting passage of a particle of the sample from the reservoir to the foyer via a first sensor disposed within the microfluidic path, and detecting passage of the particle into the target location via a second sensor disposed between the first sensor and the nozzle. The method includes recording in a dispense map, an indication of whether the target location includes a single particle or multiple particles based on signals measured by the first sensor and the second sensor.

The present disclosure relates to methods for assaying and controlling micro-objects in a microfluidic device. In situ-generated hydrogel barriers are provided for dividing a microfluidic chamber into areas where assaying a cell may be performed without interference from the presence of the cell itself.

The application provides a method for monitoring the effect of a drug on the interaction between a biological body and a fluorescent probe, the method comprising: continuously injecting a first solution comprising a fluorescent probe into a fluorescent chamber and a microfluidic chamber of a microfluidic chip; the fluorescent probe is at least one in kind; at a first time, continuously injecting a solution comprising the fluorescent probe and a drug into the fluorescent chamber and the microfluidic chamber; the drug is at least one in kind; based on the imaging information corresponding to the fluorescent chamber and the microfluidic chamber at each time unit respectively, determining the effect of the drug on the interaction between the biological body and the fluorescent probe; wherein the microfluidic chamber comprises the biological body.

The invention discloses a bionic system which comprises a plurality of micro-channel modules and a plurality of air pumps, and each micro-channel module comprises a micro-channel wafer and a liquid storage unit. The micro-channel wafer is used for bearing cells; the liquid storage unit is arranged above the micro-channel wafer, is communicated with the micro-channel wafer, and comprises at least two micro-flow cavities. And the plurality of air pumps are respectively connected with each micro-channel module. Each air pump comprises at least one air hole communicated with the liquid storage unit.

The present invention relates to the field of new material research and development, and is a micro-liquid mixing reaction device comprising a computer, a laser, a lens group, a camera, a base, a rotary motor, a rotary shaft, a rotary plate, a liquid mixer and a limit screw. The liquid mixer comprises a glass slide, a sample pool I, a sample pool II, a microchannel I, a microchannel II, a mixing chamber, a microchannel III and a liquid storage tank. The device has a specially designed microfluidic chamber and microchannel structure, adopts a mixing method based on centrifugal force, can quickly mix reactant liquids with volumes from nanoliter to microliter, and can be used for mixing and reacting small-volume fluid reaction samples. The microfluidic unit based on centrifugal force can be used for continuous mixing and reacting small-volume fluid samples of less than 30 microliters. The device has a good mixing effect on small-volume fluid samples, is low in cost, and is easy to operate.

The present application discloses a method for manufacturing a surface acoustic wave microfluidic chip with uniform near-field, which belongs to the field of microfluidics. In response to the problem of uneven lateral distribution of the acoustic field amplitude excited by the straight interdigital transducer in the existing surface acoustic wave microfluidic chip, the present application proposes a method for manufacturing a surface acoustic wave microfluidic chip with uniform lateral distribution of the acoustic field. Specifically, first, a model for calculating the acoustic field distribution of the aperture-varied interdigital transducer is constructed; further, the acoustic field distribution when the transducer fingers are weighted differently is calculated; further, an optimization algorithm is applied to obtain a weighting method that makes the acoustic field distribution in the near-field area uniform; finally, a microfluidic cavity is manufactured and installed in the near-field area of ​​the interdigital transducer. The acoustic field in the working area of ​​the device has good lateral invariance and is applicable to different piezoelectric substrate materials and microfluidic cavity structures. Compared with existing devices, the surface acoustic wave microfluidic chip of the present application can effectively improve the efficiency and accuracy of particle manipulation.

The invention discloses a magnetic microsphere sorting device which comprises a base and a rectangular shell, the rectangular shell is installed on the base, a micro-flow cavity is defined between the inner side of the rectangular shell and the base, an inlet of the micro-flow cavity is formed in the upper end face of the first end of the rectangular shell, and an outlet of the micro-flow cavity is formed in the second end of the rectangular shell. The distance between the upper wall surface in the micro-flow cavity and the substrate is gradually reduced from an inlet to an outlet of the micro-flow cavity, an electromagnet is arranged on the outer side of the first end of the rectangular shell, the side, close to the inlet, of the micro-flow cavity is parallel to the electromagnet, the outlet of the micro-flow cavity is communicated with the lower end of a cavity channel, and the upper end of the cavity channel extends to the upper end face of the second end of the rectangular shell; the upper end of the cavity channel is communicated with the liquid collecting bottle through a pipeline. According to the method, multiple marker objects in the to-be-detected sample can be detected at the same time, size sorting can be carried out, the condition of a patient is determined through strip image features, and the detection efficiency is high.

A method for displacing a micro-object from a chamber of a microfluidic device is provided. The present disclosure relates to systems, methods, kits, and computer-readable media for assaying and controlling micro-objects in microfluidic devices, including in situ generated hydrogel barriers within a microfluidic chamber that subdivide the chamber into areas capable of generating forces suitable for displacing micro-objects from cells without interfering with the cells themselves.

Embodiments of the microfluidic device may include of an array of microfluidic cell capture chambers, each functionalized with a different antibody to recognize a target antigen, and a network of code-multiplexed Coulter counters placed at strategic nodes across the device to quantify the fraction of cell population captured in each microfluidic chamber. For example, an apparatus may comprise a fluid inlet port divided into a plurality of separate microfluidic paths, each separate microfluidic path configured to transport a plurality of cells, the plurality of separate microfluidic paths, each comprising a plurality of microfluidic cell capture chambers, an outlet port to discharge a merged output of cells from the plurality of microfluidic cell capture chambers, and a plurality of sensors to detect cells passing into or out of a microfluidic cell capture chamber.

The invention belongs to the field of optical sensing, and particularly relates to an echo wall functionalized micro-flow cavity hydrogen sensing system based on a laser tracking technology. An ultrahigh-Q-value WGM optical micro-flow cavity is etched on the outer wall of a silicon dioxide capillary tube, a Pt / WO3 functionalized nano-film is deposited on the inner wall of the silicon dioxide capillary tube, a functionalized material is in indirect contact with the micro-flow cavity, hydrogen reacts with the Pt / WO3 material to release heat, and the optical characteristic change of the WGM optical micro-flow cavity is caused by heat conduction; and then the laser-trigger technology is adopted to ensure that the wavelength of the sensing laser tracks the resonance displacement of the microcavity, and photoelectric heterodyne detection is adopted to accurately measure the position and displacement of the resonance wavelength, so that the detection of the gas concentration is realized. The sensing system provided by the invention has a significant detection limit of 5ppb and large-range detection crossing five orders of magnitude, and realizes integration of high sensitivity, large dynamic range and real-time detection of hydrogen sensing; an advanced detection method is provided for microcavity sensing, and a good basis is provided for safe and large-scale application of hydrogen.

This invention relates to the field of medical device technology, and in particular to a variable stiffness interventional catheter based on a microfluidic array, comprising: a handle, a control component, and a catheter body. The handle is connected to the control component, and the control component is fixedly connected to the proximal end of the catheter body. The catheter body has an axially extending main working chamber inside. The catheter body wall has a skeleton layer for limiting radial expansion, and a plurality of axially extending microfluidic cavities are formed in the gaps of the skeleton layer. A control switch electrically connected to the control component is installed at the upper end of the handle. The control component is used to inject or discharge pressure medium into the microfluidic cavities. This invention can adjust the overall stiffness of the catheter in real time and reversibly by injecting / discharging pressure medium into the microfluidic cavities. During delivery and navigation, the catheter body remains fully flexible, possessing excellent compliance and tracking ability, and can safely pass through ultra-torsional vascular paths.

The invention discloses a non-contact biochemical sensor for artificial local surface plasmon polaritons. The non-contact biochemical sensor comprises an artificial surface plasmon polariton transmission line; a dielectric substrate; an artificial local surface plasmon resonator array; the micro-flow cavity is arranged on the artificial localized surface plasmon resonator array and comprises a dielectric shell layer and a cavity pattern layer arranged in the dielectric shell layer, the cavity pattern layer is used for storing a solution to be detected, and the dielectric shell layer is in contact with the artificial localized surface plasmon resonator array. According to the sensor, the artificial local surface plasmon resonator array and the artificial surface plasmon transmission line are mutually coupled, and the structures simulate optical surface plasma characteristics in a microwave frequency band through a sub-wavelength periodic structure, so that strong localization and slow wave propagation of an electromagnetic field are realized; therefore, the interaction efficiency with the sample is enhanced, the interaction time of the tested sample and the electromagnetic wave is prolonged, and higher sensitivity and Q factor are provided.

This application relates to microfluidic chips and immunoassay instruments, which have a microfluidic chamber formed in the main body. The microfluidic chamber includes a first valve-controlled structure, a second valve-controlled structure, a third valve-controlled structure, a fourth valve-controlled structure, a first liquid chamber, a second liquid chamber, a first reaction chamber, a detection reaction chamber, and a waste liquid chamber. The first liquid chamber, the first valve-controlled structure, the first reaction chamber, the fourth valve-controlled structure, and the waste liquid chamber are sequentially connected and arranged from closest to furthest from the center of rotation. The second liquid chamber, the second valve-controlled structure, the detection reaction chamber, the third valve-controlled structure, and the waste liquid chamber are also sequentially connected and arranged. By designing a matching first liquid chamber, second liquid chamber, first reaction chamber, and detection reaction chamber, it is suitable for time-delayed one-step and two-step detection schemes requiring precise quantification of two reactions. This allows the microfluidic chip to be matched with time-delayed one-step and two-step immunoassay reactions, further broadening the versatility of microfluidic chips, especially disk-type microfluidic chips.

Devices for high throughput cell electroporation include a trapping component that at least partially defines an upper boundary of a microfluidic chamber. A cell trap array is patterned on the underside of the trapping component, and a channeling component is positioned beneath the trapping component. The channeling component includes a vertically oriented nanochannel array. The trapping component and the channeling component are positioned such that a given nanochannels is positioned beneath a cell trap. During use, fluid flow holds trapped cells in secure contact with the nanochannels beneath the cell trap. The device further includes upper and lower electrode layers for generating an electric field to electroporate trapped cells via the nanochannel array. A reservoir positioned beneath the channeling component can be filled transfection reagent solution. During electroporation, the transfection reagent solution travels through the nanochannel array during to transfect the trapped cells.

The application discloses a surface acoustic wave microfluidic acoustic tweezers device and method, and belongs to the technical field of microfluidics. Mainly includes: surface acoustic wave excitation module, application function module, impedance matching module, coupling agent. The surface acoustic wave excitation module is composed of an interdigital transducer and a piezoelectric wafer, and the application function module is composed of a separate working substrate or a working substrate with a microfluidic channel. The surface acoustic wave excitation module excites surface acoustic waves on the piezoelectric wafer, and excites Lamb waves in the application function module through the coupling agent, thereby realizing the arrangement, manipulation, sorting or mixing, centrifugation and other operations of microparticles or cells. In view of the problems that the existing surface acoustic wave microfluidic acoustic tweezers needs complex setting and is difficult to be reused, the device can use different module combinations according to different microfluidic application requirements, realize efficient excitation of surface acoustic waves in any direction in the microfluidic working area, and realize multifunctional and modular microfluidic acoustic tweezers application.

Embodiments of a system for improved interrogation of shunt functionality are disclosed. The system includes a fluid flow detector having a microfluidic chamber configured for receiving the passage of bodily fluid flow. The fluid flow detector generates measurements and other data and provides wireless access to the same.

A deformation component including a first electrode pair, an insulating layer, a dielectric fluid and a deformation layer is provided. The insulating layer is disposed between the first electrode pair to define a microfluidic chamber, the dielectric fluid is located between the first electrode pair and in the microfluidic chamber. The deformation layer is disposed on the first electrode pair and the dielectric fluid, wherein the dielectric fluid contacts the first electrode pair, the insulating layer and the deformation layer. A haptic device including the above-mentioned deformation component, a vibrotactile component, and an electrostatic friction component is provided, wherein the vibrotactile component and the electrostatic friction component are respectively disposed on two opposite sides of the deformation component.

A structure for sensing material movement includes a set of first conductive electrodes in a top substrate and a set of second conductive electrodes in a bottom substrate positioned adjacent to and spaced apart from the set of first conductive electrodes in a top substrate. A microfluidic chamber is defined within a space between the top substrate and the bottom substrate. A first set of through vias in the top substrate connect the set of first conductive electrodes to a set of first signal lines on a top side of the top substrate. A second set of through vias in the bottom substrate connect the set of second conductive electrodes to a set of second signal lines on the bottom side of the bottom substrate.

The invention discloses a method for evaluating temperature consistency in a micro-fluidic chamber based on melting peak temperature, and belongs to the technical field of micro-fluidic chips. According to the invention, each microfluidic chamber is directly filled with the same dye method fluorescent PCR system, the melting peak temperature is read after amplification, and the indirect-in-situ temperature measurement is realized by using the corresponding relation between the temperature and the real temperature of the liquid without inserting a temperature sensor or damaging the chip seal. During an experiment, only sybr green or other dye systems need to be injected into a to-be-measured chamber, after PCR circulation is completed, a melting curve is collected within the range of 60-95 DEG C, temperature distribution of all chambers of a whole chip can be obtained at a time, and the error is remarkably lower than that of traditional infrared surface temperature measurement. The whole set of process is simple and convenient to operate, lossless and high in pass, and can meet the microfluidic application of digital PCR, LAMP and RPA isothermal amplification with extremely high requirements on the temperature consistency of the chamber.

The embodiments provide a bioengineered artificial functional liver which is connected to a patient suffering from acute liver failure and would functional like an ectopic liver. The device uses the cells derived from the patient's own body thereby nullifying the chances of self / non-self-recognition and related immune activation and rejection. The extracted liver cells are grown on a customized 3D matrix called as 3D cell cartridge and these cell cartridges individually function as miniature liver assemblies. Multiple such assemblies when working in parallel would rescue the condition of liver failure. A microfluidic chamber is built with the similar network as found in the liver and the chamber has flow circuits for plasma / de-cellularised blood and the flow circuits are lined by a coculture of hepatocytes, endothelial cells and fibroblasts. The array of cells in the chamber serve as a miniature liver and multiple such arrays will be stacked to achieve a significant hepatic function.

The present invention discloses a liquid volume precision control system driven by a microfluidic cavity, which is mainly composed of a liquid storage area, a liquid inlet area, and a liquid outlet area; the liquid storage area is composed of a liquid storage cavity and a microchannel, the liquid inlet area is composed of a microchannel, a one-way valve, an onboard microfluidic pump, and a liquid inlet pipe, and the liquid outlet area is composed of a liquid outlet pipe, a microchannel, a one-way valve, a capillary channel, and an onboard microfluidic pump, and the liquid storage area is connected to the liquid inlet area and the liquid outlet area. The present invention can achieve precise control of micron-level liquid volume in both the liquid inlet and the liquid outlet, so as to improve control accuracy and response speed, while reducing system complexity and maintenance costs.