Microfluidic chamber assembly for mastitis assay

a microfluidic chamber and mastitis technology, applied in the field of microfluidic chambers, can solve the problems of increased labor costs, reduced sale value of culled cows, and early replacement of affected cows, and achieve the effect of small volume of milk

a microfluidic chamber and mastitis technology, applied in the field of microfluidic chambers, can solve the problems of increased labor costs, reduced sale value of culled cows, and early replacement of affected cows, and achieve the effect of small volume of milk

US20090233329A1Inactive Publication Date: 2009-09-17ADVANCED ANIMAL DIAGNOSTICS
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  • Microfluidic chamber assembly for mastitis assay
  • Microfluidic chamber assembly for mastitis assay
  • Microfluidic chamber assembly for mastitis assay

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Assay for Milk Somatic Cell Differential Counts

[0042]In one embodiment of the assay, 80 Ī¼l of milk is mixed with 20 Ī¼l of a meta-chromatic stain, gently mixed, and a small drop of the mixture is placed in the deposition well of a slide of the invention. The wedge of the slide chamber fills automatically by capillary action, the cells in the milk are distributed evenly at optimum locations, and are ready for observation in seconds. A pre-concentration step may be required for very low SCC samples. The wedge can be aptly described as a ā€œself preparing wet smear.ā€

[0043]Once the wedge slide has self-prepared, it is ready for immediate analysis by one of three methods:

(a) Visual identification by direct observation of the various live, intact, fluorescing cells, using a simple fluorescence microscope (for use by the experienced milk researcher); (b) Visual identification of the various cells using computer-enhanced digital camera images in a computer screen (for the use of a laboratory c...

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Abstract

The present invention relates to a device and method for the detection of mastitis or other disease from a body fluid of a mammal for example from cow's milk. The device and method relates to a wedge microfluidic chamber for using a minimal amount of fluid and being able to use the device to observe leukocytes in a mono-layer for the purpose of disease detection, cell counts or the like.

Description

[0001]This application claims priority of U.S. provisional patent application No. 60 / 785,877 filed on 24 Mar. 2006 incorporated herein by reference in its entirety.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates to microfluidic chamber for analyzing a subjects' body fluids, in particular milk, to determine if the subject has a bacterial infection such as bovine mastitis. Specifically, the invention relates to a chamber assembly which can be used to detect mastitis or other bacterial disease in bovine or other mammal species.[0004]2. Description of the Related Art[0005]Mastitis is the inflammation of the mammary gland caused by microorganisms that invade one or more quadrants of the bovine udder, multiply, and produce toxins that are harmful to the mammary gland. Economic loss to mastitis in the United States is estimated to be approximately $185 / cow annually. The total annual US cost of mastitis is over $ 2 billion. This is approximately...

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Application Information

Patent Timeline
17 Sep 2009
Publication
US20090233329A1
IPC
C12Q1/06; C12M1/34
CPC
B01L3/5027; G01N15/1463; G01N33/04; G01N2015/008; G01N1/2813; G01N15/1484; G01N2015/016; G01N15/1433
Inventors
RODRIGUEZ, RODOLFO R.; GALANAUGH, CHARLES F.