35 results about "Mammary gland" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to a lactobacillus rhamnosus fermentation composition, a preparation method and application of the lactobacillus rhamnosus fermentation composition in the aspects of improving the tumor cell induced inflammation level and preventing and inhibiting breast tumors. The invention provides a lactobacillus rhamnosus fermentation composition. The lactobacillus rhamnosus fermentation composition is prepared from a raw material and lactobacillus rhamnosus NKU ML1-2, the raw materials comprise raspberry, poria cocos, cape jasmine, rehmannia, liquorice and orange peel. The lactobacillus rhamnosus fermentation composition has the effects of nourishing yin and clearing heat, soothing liver and regulating qi, and strengthening spleen and stomach. Results of embodiments show that the lactobacillus rhamnosus fermentation composition can prevent proliferation, invasion and migration of breast cancer tumor cells to a certain extent, and has an improvement effect on the level of inflammation induced by the tumor cells.

The invention discloses a traditional Chinese medicine formula for treating hyperplasia of mammary glands and breast cancer and a preparation method thereof. The formula comprises radix paeoniae rubra, pseudo-ginseng, radix aucklandiae, swim bladder, angelica sinensis, blister beetle, rhizoma atractylodis macrocephalae and scorpion which are compatible according to a specific proportion. The preparation method comprises the following steps: adding water to soak the five medicinal materials such as red paeony root (1 hour in summer and 2 hours in winter), adding the swim bladder, decocting until the liquid medicine is sticky, grinding the baked cantharides and scorpion into powder, and mixing. Based on theories such as the holism concept of traditional Chinese medicine, multiple modules take effect synergistically, microcirculation can be improved, immunity can be enhanced, cancer cell apoptosis can be induced, the total effective rate for hyperplasia of mammary glands is 96.7%, and the total effective rate for I-II stage breast cancer reaches 86.7%. The traditional Chinese medicine is flexible to take, high in safety and suitable for long-term treatment.

The disclosure relates to solid and liquid compositions for reducing lactation, improving health, reducing the risk and or occurrence of intramammary infections, dry-off related stress, dry-off related inflammation, and / or dry-off related infections. A preferred compound is di-n-propyl disulfide.

The breast biopsy tissue collecting and cleaning warehouse comprises 12 collecting cells and an outer warehouse. The outer warehouse is provided with 12 sub-grades, and the 12 collecting cells are respectively arranged in the 12 sub-grades. One end of the collecting cell is provided with an entrance for the biopsy tissue to enter the inside of the collecting cell. A long hole is formed in one side of the collecting cell for the biopsy tissue to be taken out from the inside of the collecting cell. The outer warehouse is provided with a plurality of cleaning ports for spraying cleaning liquid into the inside of the collecting cell to clean the biopsy tissue. The single tissue cut by rotation can be directly transported into the collecting cell in the collecting warehouse. The flushing function of the collecting warehouse can be used to flush the tissue clean. The risk that the single tissue is excessively contacted and adhered due to being concentrated together to damage the integrity of the tissue is avoided. The tissue does not need to be flushed again, the use steps of the medical staff are reduced, and the efficiency is greatly improved.

The invention belongs to the technical field of biology, and particularly relates to application of creatine in relieving heat stress injury of bovine mammary epithelial cells. It is innovatively found for the first time that creatine can improve the activity of bovine mammary epithelial cells suffering from heat stress, reduce the apoptosis amount, caused by heat stress, of the bovine mammary epithelial cells, inhibit expression of pro-apoptosis genes and promote expression of anti-apoptosis genes, and the method provides a new method for relieving or treating bovine heat stress injury, and has the advantages that the creatine is used for treating bovine heat stress injury; the composition can also be used as a protective agent for cattle to resist heat stress.

The invention discloses nucleus eliminating granules which are prepared by the following steps: adding water into 12 medicinal materials, namely selfheal, oyster, kelp, beautiful sweetgum fruit, fructus aurantii, cowherb seed, salvia miltiorrhiza, curcuma zedoary, ligusticum wallichii, white paeony root, corydalis tuber and radix bupleuri, heating, extracting, filtering, concentrating and drying to obtain medicinal powder; and sieving, adding auxiliary materials, adding a wetting agent, preparing a soft material, granulating, and drying to obtain the kernel eliminating granules. The invention further discloses a preparation method of the nucleus eliminating particles, a thin-layer chromatography identification and content determination method and application of the nucleus eliminating particles. According to the invention, the nucleus-eliminating mixture is developed into the nucleus-eliminating particles, the forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the nucleus-eliminating particles all meet the requirements of Chinese Pharmacopoeia, and the stability experiment result shows that the product stability is good; the curative effect of the nucleus eliminating granules on breast pain, breast nodules, hyperplasia of mammary glands and breast diseases can be achieved; doctors realize accurate medication under treatment based on syndrome differentiation, and the flexibility of adjusting prescriptions along with symptoms is guaranteed; on the other hand, more convenient carrying and taking experience can be provided for the patient.

The invention discloses a breast cancer treatment scheme decision-making method fusing agent cooperation and game theory modeling, and relates to the technical field of intelligent medical treatment, and the method comprises the steps: inputting a multi-sequence MRI image into an expert network, and obtaining a molecular typing result; according to a molecular typing result, a diagnosis and treatment guide and clinical text information of the patient, dividing into corresponding risk grades; adopting a Stackelberg game model to formulate a treatment scheme corresponding to the risk level, and for the low risk level LRT, formulating a treatment scheme through a single expert model; aiming at the middle risk level MRT, making a treatment scheme by adopting a prison dilemma game model; and aiming at the high-risk-level HRT, a treatment scheme is formulated by adopting a hunting deer game model. According to the method, the accuracy and reliability of breast tumor diagnosis decision are improved by combining the multi-modal patient information, the three-layer risk level, the large language model and the game theory driven multi-agent cooperation.

This invention relates to the field of image analysis technology, specifically to a method for prognostic assessment of breast and thyroid diseases combined with AI analysis of pathological images. The method includes the following steps: acquiring pathological slide images and performing grayscale statistics to generate tissue coordinate data; calculating the edge gradient and contour features of pixel neighborhoods; quantifying the morphological aberration features of cell nuclei and constructing a nuclear population potential field; analyzing the potential difference and gradient inversion regions; correcting directional consistency based on the gradient magnitude distribution direction; and finally generating a disease prognostic assessment result. In this invention, stable tissues are screened and coordinate data is generated through grayscale statistics and threshold segmentation of pathological slide images; edge gradients are calculated and closed contours are extracted; cell nucleus axis ratios and contour roughness are quantified; a regular grid is established and distance attenuation is introduced to form a nuclear population potential field; and the potential difference and gradient inversion regions are statistically analyzed and directional consistency is corrected, thereby improving the stability and consistency of the prognostic assessment.

The invention relates to the technical field of molecular biology, in particular to an MUC15 gene segment, expression vector construction of the MUC15 gene segment and an expression vector transfection method of the MUC15 gene segment, and the nucleotide sequence of the MUC15 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1. The MUC15 gene segment is obtained by amplifying a primer pair, and the primer pair is used for amplifying the buffalo mammary epithelial cell triglyceride and fatty acid synthesis. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGTTGACTCAGCCAAAATTCTG-3', F: 5 '- And R: 5 '-AATTCGAAGCTTGAGCTCGAGCTTACACGAAGTGGAGG-3'. Compared with the prior art, the buffalo mammary epithelial cell MUC15 gene fragment influencing the synthesis of triglyceride and fatty acid in the buffalo mammary epithelial cell is obtained, and a pEGFP-C1-MUC15 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.

The application discloses a borneol acetate inclusion compound for relieving apoptosis of dairy cow mammary glands, and a preparation method thereof. The preparation method comprises the following steps: (1) preparing modified cyclodextrin; (2) stirring and dissolving the modified cyclodextrin in deionized water to obtain a cyclodextrin solution; (3) stirring and dissolving borneol acetate in ethanol to obtain a borneol acetate solution; (4) adding the borneol acetate solution into the cyclodextrin solution under stirring, heating the mixture to 55 DEG C in a water bath after the feeding is completed, continuously stirring the mixture at constant temperature for more than 3 hours, condensing and refluxing the mixture in the stirring process, and storing the mixture in a refrigerator after the stirring is completed, and then vacuum drying the mixture to obtain the borneol acetate inclusion compound. The cell viability of a group to which the borneol acetate inclusion compound prepared by the application is added is better than that of a control group, and the cell viability of the group is significantly increased at 12 hours. The milk yield of the group to which the borneol acetate inclusion compound is added is significantly higher than that of the control group at the fourth week and the eighth week. The borneol acetate inclusion compound prepared by the application has a good technical effect of relieving apoptosis of dairy cow mammary glands.

1. Name of the product in this design: Breast Detection Instrument. 2. Purpose of this design: This design is used for breast cancer detection. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention discloses a wearable anti-clogging effusion drainage apparatus based on breast thyroid surgery, and belongs to the technical field of effusion drainage apparatuses, the wearable anti-clogging effusion drainage apparatus comprises a diversion head, the tip of the diversion head is provided with a cross-shaped flexible wearing paste, the free end of the diversion head is provided with a diversion mechanism with a main diversion tube, one end of the main diversion tube is communicated with the diversion head, and the other end of the main diversion tube is communicated with the diversion head. A flow dredging mechanism is arranged on the outer side of the main flow guide pipe and comprises a connecting pipe installed on the outer surface of the main flow guide pipe in a communicating mode, dredging steel wires slidably penetrate through the connecting pipe and the main flow guide pipe, and a rotating wheel is rotationally installed on the outer surface of the connecting pipe. The dredging steel wire capable of being driven by the driving wheel to move in a reciprocating mode is arranged in the main drainage pipe, blood clots, fiber flocks and tissue fragments generated in the drainage process can be broken in real time in cooperation with continuous stirring of the arc-shaped stirring blades in the liquid storage cavity on hydrops, pipeline silting and blocking are avoided from the source, continuous and smooth drainage is guaranteed, and the drainage effect is improved. And drainage interruption and secondary operation caused by blockage are greatly reduced.

A method of producing mammalian breast milk exosomes, such as human breast milk exosomes, comprising generating lactating cells derived from mammalian breast epithelial cells, such as human breast epithelial cells, expressing mammalian milk-like products, such as human milk-like products, from the lactating cells, and purifying the exosomes from the mammalian milk-like products.

The invention discloses an MRI breast tumor segmentation method based on a frequency perception and deformation attention fusion network. The MRI breast tumor segmentation method comprises the following steps: (1) constructing a 3D segmentation network based on an improved U-Net architecture; (2) in the down-sampling stage of the network encoder, a learnable local detail residual error down-sampling module is adopted, and high-frequency edge information of a tiny tumor is reserved through a frequency gating mechanism; and (3) the bottleneck layer adopts a deformable axial attention module, and local deformation adaptation and global context perception are combined to improve the segmentation precision of the irregular tumor. And (4) optimizing network parameters by adopting a weighted combination of Dice loss and cross entropy loss in a training stage, and performing resampling and intensity normalization preprocessing on the MRI data in a reasoning stage to obtain a complete volume segmentation result through reasoning. According to the method, the problems of small target feature loss and poor tumor form adaptability are effectively solved, so that the accuracy of breast tumor segmentation is improved.

The present application relates to the technical field of feed additive, in particular to a kind of compound additive for improving lactation of sow and its preparation method and application, the compound additive is the polysaccharide skeleton of xanthan gum and konjac glucomannan, and active component is loaded in the polysaccharide skeleton inside;Wherein, the active component includes organic acid, prebiotic, functional amino acid, compound mineral, grass juice concentrate and fish solution.The present application has the advantages that polysaccharide skeleton microspheres are prepared by compounding xanthan gum and konjac glucomannan, which can realize low release of active ingredients in stomach, high release in intestinal tract, and help to improve the utilization efficiency of active ingredients.The combination of fructo-oligosaccharides, taurine and arginine plays a role in intestinal health, antioxidant, mammary blood vessel dilation and milk protein synthesis, etc., forming a synergistic lactation effect.Microspheres embedding has a protective effect on water-soluble components such as fructo-oligosaccharides, taurine and arginine, which can reduce their loss during feed processing and storage.The raw materials of the present application are all of natural origin, which meets the requirements of green feed additive.

The invention discloses a liquid medicine for conditioning breast diseases. The liquid medicine comprises the following raw materials by weight: 12-22 g of garden burnet; 15 to 25 g of prunella vulgaris; 17 to 27g of seaweed; and 14-24g of fructus liquidambaris. The raw materials of the medicine liquid for conditioning breast diseases are medicinal and edible or traditional Chinese medicinal materials, so that the medicine liquid is small in side effect and suitable for long-term conditioning; the traditional Chinese medicine composition has a direct treatment effect on hyperplasia of mammary glands and breast distending pain before menstruation, and can be used as a mild auxiliary conditioning means for more serious mammary gland diseases.

The invention belongs to the technical field of biology, and particularly relates to application of baicalin to preparation of a product for promoting milk fat synthesis of cow mammary epithelial cells. Researches show that baicalin can promote expression of milk fat synthesis related genes in bMECs and formation of lipid droplets, then milk fat synthesis is promoted, and the influence of oxidative stress on milk fat synthesis of bMECs is relieved. In addition, baicalin can promote the level of key protein in milk fat synthesis in bMECs.

There are described various methods and systems for automatic assessment of breast morphology using three-dimensional (3D) sensor data of a breast area collected in situ using a time-of-flight (ToF) sensor. The 3D sensor data is processed to determine one or more breast morphology parameters in respect of one or more breasts in the breast area. The methods and systems also provide graphical outputs comprising 3D visualizations of the stacking of the overlayed regions of the breast morphology and "Volumetric and Iso-contour based Morphological Asymmetry" (VIMA) scores for automatically and objectively assessing breast asymmetry.

The invention discloses a method for detecting rabbit DNA residual quantity in a rabbit-derived biological product, and belongs to the field of DNA detection. The rabbit DNA in the recombinant human C1 esterase inhibitor expressed by a transgenic rabbit breast bioreactor platform can be effectively detected through the combination of specific primer sequences and probe sequences, and the rabbit DNA detection kit has good specificity, a large linear range and high accuracy, precision and sensitivity, and can be used for detecting the rabbit DNA in the recombinant human C1 esterase inhibitor expressed by the transgenic rabbit breast bioreactor platform. The method can be applied to detection of DNA residues in rabbit-derived biological products and related biological products, and has a good application prospect.

The invention relates to the technical field of molecular biology, in particular to an ACSL3 gene segment, an expression vector construction method thereof and an expression vector transfection method thereof, the nucleotide sequence of the ACSL3 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1, the ACSL3 gene segment is obtained by amplifying a primer pair, and the primer pair has a nucleotide sequence shown as SEQ No.2. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGAATAACCAGGGTCTACAA-3', F: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 ' And R: 5 '-AATTCGAAGCTTGAGCTATGAGCTATTTTCTTCCATATTCGCTCA-3'. Compared with the prior art, the ACSL3 gene fragment influencing the synthesis of the buffalo mammary epithelial cell triglyceride and fatty acid is obtained, and a pEGFP-C1-ACSL3 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of the triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.

To provide a formulation having a novel sustained release multi-phasic concentration pattern, particularly a formulation that can be used to reduce mammographic breast density and / or breast stiffness in warm-blooded animals.SOLUTION: For example, the formulation may include administration of an effective amount of an androgenic agent and an effective amount of an aromatase inhibitor to a subject, and provides a sustained release multiphasic concentration pattern in the blood of the subject over time, as measured by serum concentration for the androgen and plasma concentration for the aromatase inhibitor, and particularly improves breast tissue stability and / or increases androgen receptor expression levels.SELECTED DRAWING: Figure 2

A pharmaceutical formulation for treating breast hyperplasia and a preparation method therefor. The pharmaceutical formulation comprises a drug-loaded microneedle layer, a backing layer connected to the drug-loaded microneedle layer, and a medical blank adhesive patch adhered to the backing layer. The drug-loaded microneedle layer is prepared from the following raw materials: a traditional Chinese medicine compound clear paste, a microneedle matrix and purified water. The traditional Chinese medicine compound clear paste is prepared from the following raw materials: radix bupleuri chinensis, curcumae radix, rhizoma cyperi, radix paeoniae alba, frankincense, rhizoma pinelliae, bulbus fritillariae thunbergii, spica prunellae, camphor essential oil, turpentine and a 70% ethanol solution. The backing layer is prepared from the following raw materials: a backing layer matrix and purified water.

Described herein are gene features for providing prognosis, diagnosis, treatment and molecular subtype classification of cancer by genomic and epigenomic profiling, methods and compositions for determining cancer and subtypes, including breast cancer, and provide specific and sensitive detection of biomarkers of interest. Such biomarkers indicate disease pathogenesis, which provides opportunities to select treatment, including treatment regimens intended to identify responsive candidates and overcome resistance mechanisms.

The invention discloses a traditional Chinese medicine composition for treating female hyperplasia of mammary glands. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 50-70 parts of radix curcumae, 20-40 parts of pericarpium citri reticulatae viride, 20-40 parts of pericarpium citri reticulatae viride, 100-140 parts of fructus trichosanthis, 20-40 parts of ginger worms, 50-70 parts of radix paeoniae rubra, 50-70 parts of radix paeoniae alba and 50-70 parts of radix angelicae sinensis. All the raw materials are reasonable in compatibility and can effectively relieve inflammatory reaction and pain caused by hyperplasia of mammary glands through the synergistic effect, and meanwhile adjustment and recovery of a body immune system can be promoted. The traditional Chinese medicine preparation is a pure traditional Chinese medicine preparation and has little harm to the body and few side effects.

In alternative embodiments, provided are methods comprising use of orthotopic and organoid models to better characterize ADAR1 activity in breast cancer (BC) metastatic niches, with a special focus on the less studied brain metastasis (BM). Methods as provided herein provide new insights into the mechanistic role of ADAR1 in breast metastatic disease and provide novel models for developing or discovering new therapeutics, such as as drugs, effective against BC and BM.

The present invention provides in vitro methods of for the production of milk-like products in a bioreactor. The method comprising providing a bioreactor comprising a culture vessel and a membrane insert that divides the culture vessel into an apical compartment and a basal compartment. A confluent layer of mammary-derived cells, optionally mammosphere-derived cells, is adhered to the apical facing surface of the membrane. Lactation media is provided to the basal compartment, optionally the lactation media comprises TGFb inhibitor, an inhibitor of intracellular actomyosin contractility such as a ROCK inhibitor or a myosin II inhibitor, and / or prolactin. Milk-like product is isolated from the apical compartment. Optionally a priming treatment is added to growth media of the hyperconfluent cell monolayer before switching to lactation.

The invention provides an epigenetic editing method for improving nutritional ingredients of cow milk by activating lactation gene expression, and belongs to the technical field of gene engineering. According to the invention, sgRNA plasmids are respectively mixed with pCMV-dCas9-VPR-GFP and pCMV-dCas9-P300-mCherry plasmids, the mixture is mixed with a P3000 culture medium and an MEM culture medium to obtain diluted nucleic acid, lip3000 and the MEM culture medium are mixed to obtain a diluted reagent, and the diluted nucleic acid and the diluted reagent are mixed, incubated and transfected to cells. By co-transfecting an epigenetic regulation tool plasmid and a targeted sgRNA plasmid, an epigenetic editing system in the cattle cells is established, the epigenetic editing system can specifically act on a cis-action region of a cattle mammary epithelial cell lactation character gene, and the mRNA expression level of a target gene is remarkably improved, so that the content of nutritional ingredients in cow milk is effectively increased.

Described herein are gene signatures providing prognostic, diagnostic, treatment and molecular subtype classifications of cancers through genomic and epigenomic profiling, Methods and compositions for determining cancer and subtypes, including breast cancer are described and specific and sensitive detection of biomarkers of interest is provided. Such biomarkers are indicative of disease pathogenesis, which provides opportunity for selection of treatment, including treatment regimes directed at identifying candidates for responsiveness and overcoming resistance mechanisms.

The application discloses a liposome curcumin composition for assisting in regulating nodular diseases and application thereof, and belongs to the technical field of health foods. The composition is composed of 60-80 nm liposome curcumin, 3-10 g / portion of inulin, 10-80 mg / portion of cardamom extract, 100-1000 mg / portion of myo-inositol and 20-100 mu g / portion of selenium element. The liposome is prepared by using food-grade phospholipid, the encapsulation efficiency is greater than or equal to 80%, the ethanol residue is less than or equal to 0.5 ‰, the release rate in a pH 2.0 simulated gastric juice for 2 hours is less than 33%, and the release rate in a pH 7.4 simulated intestinal juice for 4 hours is greater than 80%. The application is based on the theory of treating different diseases in the same way, and four types of nodular core pathogenesis, such as inflammatory accumulation, endocrine disorder, oxidative stress and immune imbalance, are simultaneously intervened by the synergistic effect of multiple components, so that the synchronous auxiliary regulation of thyroid, breast and uterus multiple types of nodules is realized. The product fills the blank of lack of effective intervention means in the clinical regular observation period, and is suitable for the daily regulation of patients with various nodular diseases and the use for preventing recurrence after operation.

The application belongs to the technical field of medical equipment, and particularly relates to a breast nursing device, which comprises two protection assemblies, one round pin is fixedly connected to each side of the two protection assemblies away from each other through two protruding blocks, an elastic band is movably sleeved outside each of the two round pins, and magic tapes that are used in cooperation are arranged at the ends of the two elastic bands away from the protection assemblies. The protection assembly is arranged, the inner cover and the outer cover are arranged in a hollow shape, the chest after operation can be protected, the chest is prevented from being collided or pressed by external force, the air permeability of the wound after operation is ensured, and the recovery of the wound is facilitated. The close-fitting assembly and the transposition mechanism are arranged, the air bag can be attached to the chest of the patient and pressurized under the premise of ensuring the air permeability of the wound, the skin flap can be attached to the chest wall, and the accumulation of liquid and air is prevented.