3 results about "Breast tissue sample" patented technology

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent specifically binding to OARD1 protein in preparation of a breast cancer diagnosis and / or prognosis evaluation product. The product detects the expression level of OARD1 protein in a breast tissue sample by the reagent specifically binding to OARD1 protein and carries out H-score scoring, takes a preset H-score score as a breast cancer positive diagnosis threshold, and judges whether the sample is breast cancer positive. Moreover, the product can distinguish high / low expression of OARD1 protein by a preset H-score score, constructs a prognosis prediction model in combination with TNM staging of a subject, and realizes evaluation of a breast cancer patient disease progression risk. The method is simple and easy to implement, the diagnosis process is safe and effective, is easy to be accepted by patients, diagnosis standards are unified, and is less affected by personal subjective factors, which shows that OARD1 protein has important significance for diagnosis or prognosis judgment of breast cancer.

Provided herein according to some aspects is a method for processing a tissue sample from a subject, the sample comprising cells of a breast tissue site comprising or suspected of comprising ductal carcinoma in situ (DCIS), and detecting an expression level of a plurality of genes in the cells. Also provided according to some aspects is a method for generating a classifier capable of determining a risk of DCIS recurrence and / or progression. Further provided is a system for determining the risk of DCIS recurrence and / or progression in a subject in need thereof.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a reagent specifically combined with OARD1 protein in preparation of a breast cancer diagnosis and / or prognosis evaluation product. According to the product, the expression level of the OARD1 protein in a breast tissue sample is detected through a reagent specifically combined with the OARD1 protein, H-Score scoring is performed, and whether the sample is positive of breast cancer or not is judged by taking a preset H-Score score as a breast cancer positive diagnosis threshold value. High / low expression of the OARD1 protein can be distinguished according to a preset H-Score score, and a prognosis prediction model is constructed by combining TNM stages of a subject, so that the disease progression risk of a breast cancer patient is evaluated. The method is simple and easy to implement, the diagnosis process is safe and effective, the method is easily accepted by patients, the diagnosis standard is uniform, the influence of personal subjective factors is small, and it is indicated that the OARD1 protein has important significance on diagnosis or prognosis judgment of breast cancer.