6 results about "Breast tissue sample" patented technology

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent specifically binding to OARD1 protein in preparation of a breast cancer diagnosis and / or prognosis evaluation product. The product detects the expression level of OARD1 protein in a breast tissue sample by the reagent specifically binding to OARD1 protein and carries out H-score scoring, takes a preset H-score score as a breast cancer positive diagnosis threshold, and judges whether the sample is breast cancer positive. Moreover, the product can distinguish high / low expression of OARD1 protein by a preset H-score score, constructs a prognosis prediction model in combination with TNM staging of a subject, and realizes evaluation of a breast cancer patient disease progression risk. The method is simple and easy to implement, the diagnosis process is safe and effective, is easy to be accepted by patients, diagnosis standards are unified, and is less affected by personal subjective factors, which shows that OARD1 protein has important significance for diagnosis or prognosis judgment of breast cancer.

The invention provides a mammary tissue thermal conductivity measuring method and device based on a transient hot filament method, and relates to the technical field of biological tissue thermal measurement. The method comprises the following steps: providing a mammary tissue sample, selecting a hot filament of which the effective length is smaller than that of the mammary tissue sample, and completely wrapping the mammary tissue sample with the hot filament; direct current is connected to the two ends of the hot wire, the change relation of the voltage at the two ends of the hot wire along with time is collected, and the change relation of the hot wire temperature along with time is obtained according to the linear relation of hot wire resistance and temperature; and based on a preset formula, obtaining the thermal conductivity of the mammary tissue sample according to the change relation of the hot filament temperature along with time. The thermal conductivity of the mammary tissue can be accurately measured, the requirement for accurately regulating and controlling the temperature is met, and parameter selection and scheme optimization are provided for photothermal therapy of the breast cancer.

The invention relates to the field of biomedicine, and discloses a method for prevention, early diagnosis and treatment strategy of breast cancer, which comprises the following steps: step 1, detecting the utilization rate and the protein expression level of a far-end polyadenylation site of CDCA7L gene in a breast tissue sample or a peripheral blood sample of a high-risk group; 2, according to the abnormal change that the utilization rate of the far-end polyadenylation site is reduced and the protein expression level is up-regulated, performing prevention intervention or early diagnosis judgment on an individual with the abnormal change; and 3, constructing an intervention vector of a long 3'untranslated region of the targeted CDCA7L gene. By taking an abnormal variable polyadenylation mode of CDCA7L gene as a molecular mechanism, high-risk population intervention of a prevention link, molecular feature detection of early diagnosis and targeted intervention of a treatment link are linked, so that the three links play a synergistic role on the basis of the same biological basis.

Methods of predicting treatment benefit for reducing risk of recurrence for breast cancer is provided. In some embodiments, a method can include providing a breast tissue sample from a subject with invasive, hormone receptor (HR)-positive, HER2-negative breast cancer; analyzing the breast tissue sample for a biosignature comprising at least the biomarkers GLUT1 and Ki67 or at least the biomarkers PR and FOXA1, where the analyzed biosignature predicts the benefit from a breast cancer treatment with adjuvant therapy (e.g., radiotherapy followed by endocrine therapy) to reduce the risk of breast cancer recurrence in the subject. In some embodiments, the method includes administering the breast cancer treatment with adjuvant therapy to the subject.

Provided herein according to some aspects is a method for processing a tissue sample from a subject, the sample comprising cells of a breast tissue site comprising or suspected of comprising ductal carcinoma in situ (DCIS), and detecting an expression level of a plurality of genes in the cells. Also provided according to some aspects is a method for generating a classifier capable of determining a risk of DCIS recurrence and / or progression. Further provided is a system for determining the risk of DCIS recurrence and / or progression in a subject in need thereof.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a reagent specifically combined with OARD1 protein in preparation of a breast cancer diagnosis and / or prognosis evaluation product. According to the product, the expression level of the OARD1 protein in a breast tissue sample is detected through a reagent specifically combined with the OARD1 protein, H-Score scoring is performed, and whether the sample is positive of breast cancer or not is judged by taking a preset H-Score score as a breast cancer positive diagnosis threshold value. High / low expression of the OARD1 protein can be distinguished according to a preset H-Score score, and a prognosis prediction model is constructed by combining TNM stages of a subject, so that the disease progression risk of a breast cancer patient is evaluated. The method is simple and easy to implement, the diagnosis process is safe and effective, the method is easily accepted by patients, the diagnosis standard is uniform, the influence of personal subjective factors is small, and it is indicated that the OARD1 protein has important significance on diagnosis or prognosis judgment of breast cancer.