189 results about "Mammary tissue" patented technology

Methods and systems are provided for the noninvasive measurement of the subepithelial impedance of the breast and for assessing the risk that a substantially asymptomatic female patient will develop or be at substantially increased risk of developing proliferative or pre-cancerous changes in the breast, or may be at subsequent risk for the development of pre-cancerous or cancerous changes. A plurality of electrodes are used to measure subepithelial impedance of parenchymal breast tissue of a patient at one or more locations and at least one frequency, particularly moderately high frequencies. The risk of developing breast cancer is assessed according to measured and expected or estimated values of subepithelial impedance for the patient and according to one or more experienced-based algorithms. Devices for practicing the disclosed methods are also provided.

The present invention relates to non-steroidal progestins of the general formula (I)whereinR1 and R2 are independently of each other —H or —F,R3 is —CH3 or —CF3, andAr isor a pharmaceutically acceptable derivative or analogue thereof. These progestins are suitable for selectively modulating progesterone receptor mediated effects in different target tissues, particularly in uterine tissue versus breast tissue. Therefore, the progestins of the present invention, optionally in combination with estrogens, may be used for contraception (in particular in estrogen-free oral contraceptives), hormone replacement therapy and the treatment of gynecological disorders. The present invention furthermore relates to methods for selectively modulating progesterone receptor mediated effects in different target tissues or organs.

The present invention provides methods for producing sialyloligosaccharides in situ in dairy sources and cheese processing waste streams, prior to, during, or after processing of the dairy source during the cheese manufacturing process. The methods of the present invention use the catalytic activity of alpha(2-3) trans-sialidases to exploit the high concentrations of lactose and alpha(2-3) sialosides which naturally occur in dairy sources and cheese processing waste streams to drive the enzymatic synthesis of alpha(2-3) sialyllactose. alpha(2-3) sialyloligosaccharides produced according to these methods are additionally encompassed by the present invention. The invention also provides for recovery of the sialyloligosaccharides produced by these methods. The invention further provides a method for producing alpha(2-3) sialyllactose. The invention additionally provides a method of enriching for alpha(2-3) sialyllactose in milk using transgenic mammals that express an alpha(2-3) trans-sialidase transgene. The invention also provides for recovery of the sialyllactose contained in the milk produced by this transgenic mammal either before or after processing of the milk. Transgenic mammals containing an alpha(2-3) trans-sialidase encoding sequence operably linked to a regulatory sequence of a gene expressed in mammary tissue are also provided by the invention.

The disclosed testing block for Her2 express in mammary tissue includes: the Her2 high-express area, the express contrast area, the no-express contrast area, and the testing area for target tissue. This invention can provide accurate evaluation standard for clinic diagnosis on breast cancer.

The invention discloses a method for determining an outer edge contour of mammary gland. The method comprises the following steps: (A1) performing binarization processing on data of a mammary gland image; (A2) computing a mass center of the mammary gland image; and (A3) performing region growing by taking the mass center of the mammary gland image as an initial point, wherein, the region covered is a mammary tissue region when the region growing is completed, thus determining the outer edge contour of the mammary gland. The method for determining the outer edge contour of the mammary gland can help accurately determine the outer edge contour of the mammary gland by such image processing technologies as extraction of the image mass center and the image binarization, the region growing and the like, and the method is a significant progress in the image processing technologies.

The computer aided method of predicting mammary cancer risk includes the following steps: partitioning the mammary gland tissue area in X-ray image regularly into small areas and calculating the density characteristic value of the small areas; selecting the initial suspicious small areas with the preset density characteristic value for further research; judging the final suspicious small areas based on the information in the corresponding areas and the adjacent areas on two frames of images; and judging the near term mammary cancer risk of the testee based on the existence of the marked final suspicious small areas and predicting the mammary cancer position in case of existing final suspicious small areas. The present invention has high objectivity, high accuracy and high effectiveness in predicting mammary cancer risk.

The invention discloses a breast tumor partition method. The breast tumor partition method includes the steps of building a coupled framework of classifications and biased field correcting of breast tissue nuclear magnetic resonance images, enhancing the breast areas and the peripheral areas, and partitioning the breast tumor images in cooperation with the shape prior. By means of the breast tumor partition method, biased field information is fused into classification models, the biased field information and the classification models are combined to be the unified framework, and the classifications and a corrected biased field of the breast tumor nuclear magnetic resonance images are solved with the fast energy minimization method at the same time, and use information of each other in the model evolution process for finally achieving accurate solving of the classifications and the corrected biased field; the shapes of blood vessels and tumors are analyzed, differences of the blood vessels and the tumors in shape are caught, the shape prior is built through parameters such as the characteristic values and the characteristic vectors, and the level set driving force based on the shapes is built and combined with a level set method based on local information, so that a level set overcomes the interference of a tubular structure during evolution and only captures the breast tumor area.

The invention provides a method and a composition used for evaluating patients with breast cancer, particularly the prognosis of the patients with the breast cancer at an early stage. The method of the invention comprises the detection of at least one of body samples, particularly the expression of at least two biological markers, wherein, the over-expression of the biological markers or the combination of the biological markers indicates the prognosis of the breast cancer. The body samples in a plurality of implementation proposals are the mammary tissue samples, particularly the primary breast tumor samples. The biological markers of the invention are proteins and / or genes, and the over-expression indicates the good or poor cancer prognosis. The concerned biological markers include the proteins and genes which participate in cell cycle regulation, DNA replication, transcription, signal transduction, cell proliferation, cell invasion, protein hydrolysis or transfer. The biological marker-specific antibody is used on the protein level or the nucleic acid hybridization technology is used on the nucleic acid level for detecting the over-expression of the concerned biological markers in certain aspects of the invention.

A method of processing a mammogram image to derive a value for a parameter useful in detecting differences in breast tissue in subsequent images of the same breast or relative to a control group of such images, said derived parameter being an aggregate probability score reflecting the probability of the image being a member of a predefined class of mammogram images, comprises computing for each of a multitude of pixels within a large region of interest within the image a pixel probability score assigned by a trained statistical classifier according to the probability of said pixel belonging to an image belonging to said class, said pixel probability being calculated on the basis of a selected plurality of features of said pixels, and computing said parameter by aggregating the pixel probability scores over said region of interest. Saud features may include the 3-jet of said pixels.

A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

A reconstituted human breast tumor model is disclosed. The model, which is incorporated into mice, provides actual tumors that arise spontaneously, thereby mimicking naturally occurring breast cancer. The tumors are genetically human, because they arise from human mammary tissues that develop from human mammary epithelial cells implanted into host mice. Prior to implantation, the mammary epithelial cells are genetically modified to contain a recombinant human oncogene and an SV40 early region.

The invention relates to a novel mammary gland MRI automatic auxiliary diagnosis method based on a fusion attention mechanism. The novel mammary gland MRI automatic auxiliary diagnosis method comprises the following steps: S1, manually selecting a mammary gland segmentation data set, training a DenseUNet model, inputting a TSE sequence of mammary glands into the trained DenseUNet model for mammarygland segmentation, and removing organs interfering with tumor detection in the thoracic cavity; S2, mapping the segmentation result obtained in the step S1 to a DCE sequence, to obtaining the segmented mammary tissues, inputting the segmented mammary tissues into an ADUNet model with an attention mechanism to perform tumor segmentation, and aiming at the problems of class imbalance and difficultsamples, adopting Focal Loss in a training process to prevent the model from deviating; S3, inputting the result obtained in the S2 into a lightweight neural network, and carrying out benign and malignant judgment to obtain an auxiliary diagnosis result. According to the invention, the end-to-end breast cancer auxiliary diagnosis can be realized without manual intervention, and the diagnosis efficiency and accuracy can be greatly improved.

Methods and compositions are provided for the identification of expression signatures in ER+ breast cancer cases, where the signatures correlate with responsiveness, or lack thereof, to treatment with tamoxifen or another antiestrogen agent against breast cancer. The signature profiles are identified based upon sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for predicting the efficacy of treating a subject with breast cancer with tamoxifen or another antiestrogen agent against breast cancer. Additional methods and compositions are provided for predicting responsiveness to tamoxifen or another antiestrogen agent against breast cancer in cases of breast cancer by use of multiple biomarkers. Two biomarkers display increased expression correlated with tamoxifen response while two other biomarkers display decreased expression correlated with tamoxifen response.

The invention discloses a primary isolated culture method for dairy cow mammary epithelial cells. The primary isolated culture method comprises steps as follows: mammary tissue of a dairy cow in the lactation period is shorn into tissue blocks, the tissue blocks are digested with collagenase / hyaluronidase digestive juice, a culture dish is inoculated with the digested chylous tissue blocks for culture, cells get free from tissue on peripheries of the tissue blocks after 1-2 days and grow along the wall of the culture dish, fusiform fibroblast is removed with a mechanical scraping method every day during culture, and initial passage is performed when a large quantity of cobblestone-like mammary epithelial cells grow in clusters and in patches and are mutually fused after about 4-5 days. During initial passage, a little fibroblast which is probably mixed is eliminated with trysin with a differential digestive method, and the dairy cow mammary epithelial cells with high purity are obtained. The mammary epithelial cells can emigrate from chylous tissue in a short time; a little mixed fibroblast is removed mainly with a mechanical scraping method, and accordingly, the obtained mammary epithelial cells are seldom damaged and are high in purity.

A degradable mammary scaffold comprises a three-dimensional boundary structure and an inner filling structure; the outline of the three-dimensional boundary structure matches with actual tumor outline of an implant taking patient, the outline of the three-dimensional structure is extended by 1-5 mm along a normal direction based on the actual tumor outline, and the inner filling structure includes a three-dimensional elastic unit array that may intersect with the three-dimensional boundary structure to form the degradable mammary scaffold; after the degradable mammary scaffold is implanted in a human body, mammary form can be repaired, and good overall elasticity and good deforming characteristic to simulate natural mammary tissues are imparted; the unique porous structure of the degradable mammary scaffold helps ingrowth of tissue cells, and mammary implant separation, displacement and other risks are solved; with degrading of the scaffold, autogenous tissues replace the scaffold structure gradually, and finally self-repair is achieved.

The invention provides a new molecular marker hsa-miR-374a of breast carcinoma, that is, non-coding RNA gene hsa-miR-374a of micromolecule as a molecular marker of breast carcinoma. Expression of the molecular marker in tissues suffering from breast carcinoma is obviously higher than that of normal mammary tissues, and is associated with clinical classification of breast carcinoma, and expression of hsa-miR-374a in breast carcinoma cell line cultured in vitro is higher than that of normal mammary epithelial cells and immortalized normal mammary epithelial cells. The invention further provides application of the molecular marker of breast carcinoma to preparing an anti-breast cancer drug. The drug comprises effective amount of blocker capable of blocking expression of non-coding RNA gene hsa-miR-374a of micromolecule. The molecular marker provides new effective way for diagnosing and treating breast carcinoma. The invention also provides certain experience and foundation for further research of function of hsa-miR-374a and relation with other tumors.

The invention discloses a recombinant plasmid of a GSDMD-N (gasdmermin D-N) gene, an expression method in mammary gland and an application, and belongs to the field of genetic engineering. An expression recombinant plasmid of the GSDMD-N gene is constructed and enabled to be specifically expressed in breast tissue and breast cells, so that mastitis caused by staphylococcus aureus infection is prevented and treated. GSDMD-N protein is a polypeptide compound found in recent years, which can specifically recognize biofilm component cardiolipin of bacteria and can be combined with cardiolipin to form a plurality of honeycomb-shaped pores, so that bacteria are disintegrated and die. According to the technology, the exogenous gene GSDMD-N is efficiently expressed in animal mammary glands througha specific promoter WAP (whey acidic protein) of breast tissue by a mammary bioreactor, staphylococcus aureus causing cow mastitis is killed, and accordingly, the cow mastitis caused by staphylococcus aureus infection is prevented and treated. The recombinant plasmid and the recombinant attenuated salmonella have the advantages of being efficient, safe, low in cost and the like, provide a a new approach for prevention and control of the cow mastitis caused by staphylococcus aureus infection, and have good application prospect.

An expandable mammary tissue implant including a shell having an anterior face and a posterior face, the anterior face having an upper pole portion and a lower pole portion meeting at an apex, and an injection zone for receiving fluid therethrough to inflate the implant. The implant further includes a vertical tether member having first and second ends and a central region therebetween having an aperture therethrough. The central region is coupled to the anterior face of the implant at a location such that the injection zone is positioned within the aperture in the central region. The first and second ends of the vertical tether member are coupled to the posterior face of the implant.

The method provides a method for measuring a volume of dairy cow mammary tissue. The method includes the following steps of placing two shooting devices at a plurality of preset positions on two sides of a fence respectively, calibrating the shooting devices, collecting images as original images, acquiring a dairy cow breast image, constructing a three-dimensional description of a breast shape, calculating an upper bound depth and a lower bound depth of a mammary tissue distribution area of each breast area, acquiring three-dimensional coordinates of upper and lower bounds of the mammary tissue distribution area in a normal vector direction of an external three-dimensional point cloud, constituting inside and outside three-dimensional descriptions of the cow mammary tissue distribution area, acquiring the ratio of mammary tissue in an ultrasound image of each breast area, and calculating an actual volume of the mammary tissue of each breast area. According to the method for measuring the volume of the dairy cow mammary tissue, a cow breast three-dimensional model is constructed by means of a computer vision technology, measurement of the appearance assessment index data and volume is performed, internal tissue distribution of organs are analyzed through the ultrasound image, and real-time measurement of a proportional relation of the internal tissue distribution of cow breasts is performed.

The invention discloses an X-ray machine for checking whether human mammary tissue is normal. The X-ray machine mainly comprises a prone position bed, a bulb tube for transmitting X rays and a breast compressor, wherein the prone position bed is provided with two breast through holes; the bulb tube for transmitting the X rays is arranged on a side surface of the breast compressor; and the breast compressor and the bulb tube are arranged below the prone position bed and are positioned corresponding to the two breast through holes. A vertical frame of the traditional mammary X-ray machine is arranged as the prone position bed, so a checked person lies on the bed at a prone position, two breasts are exposed out of the two breast through holes and naturally sag due to attraction of gravitation, and the entire sagged breasts can be compressed by the breast compressor below the bed. Therefore, X-rays can permeate the breasts completely, and missed check cannot occur.

A method for producing anatomical models and models obtained are disclosed. The method includes obtaining information by diagnostic imaging; obtaining a three-dimensional, computerised model of the anatomical structure; and performing the following steps: designing a negative mould; printing the negative mould in 3D; printing in 3D rigid pieces of internal elements of the model provided; placing the pieces in the mould; closing and sealing the mould; injecting a soft material into the mould; and removing same from the mould. The anatomical model is a liver with hepatobiliary vasculature and tumours made from the rigid parts and hepatic parenchyma made from the soft material, or it is a mammary gland with a tumour and muscular tissue made from the rigid parts, mammary tissue made from the soft material and an external covering as skin.

The invention belongs to the field of an intelligent wearing technology, and specifically an intelligent corsage for detecting breast diseases. The intelligent corsage for detecting the breast diseases is provided with a signal acquisition module, a wireless communication module and a control module which are sequentially connected; the signal acquisition module comprises an infrared transmitter, an infrared receiver and a signal processor which are sequentially connected, wherein the signal processor is connected with the wireless communication module; the control module comprises a microprocessor, a memory and a buzzer, wherein the microprocessor is respectively connected with the memory and the buzzer, and the microprocessor is connected with the wireless communication module. Mammary abnormity is judged by acquiring the near infrared ray grey scale signals of mammary tissues through the intelligent corsage; by adopting the wireless communication module, an electronic circuit in the corsage can be reduced, the comfort of the corsage can be improved, and the life of washing and prolonging the corsage can be conveniently prolonged; and compared with the self-check of females through specific skills and annual periodic physical examination, the intelligent corsage is high in reliability and is capable of discovering the mammary abnormity.

The invention discloses a method for separating, purifying and culturing a mammary epithelial cell of a ruminant animal, which includes the following steps of: 1) the sampling of a mammary tissue; 2) the preparing of a culture fluid; 3) the culturing of a tissue block for obtaining a primary cultured mammary epithelial cell; 4) the purifying of the mammary epithelial cell for obtaining a purified mammary epithelial cell; and 5) the subculturing of the mammary epithelial cell. The invention simultaneously provides a preservation method for the mammary epithelial cell of the ruminant animal, which includes the following steps of: 1) preparing a freezing protection fluid which comprises the mammary epithelial cell of the ruminant animal; and 2) gradually and slowly reducing the temperature of the freezing protection fluid. The method can be adopted for prolonging the preservation time of the mammary epithelial cell of the ruminant animal.

A probe for NIR glandular tissue detection, which belongs to the field of glandular tissue detection technology, is characterized in that the probe comprises a multi-wavelength NIR light source and a plurality of photodetectors for receiving NIR light emitted by the multi-wavelength NIR light source; the multi-wavelength NIR light source is positioned on the opposite side of the topological structure constituted by the plurality of photodetectors at a distance as far as possible. The invention also provides an embodiment. In the invention, the straight line distance between the multi-wavelength NIR light source and each photodetector is increased in the small probe, so that the small probe can be used for detecting thicker glandular tissue and the detection volume is larger, thus improving the detection efficiency.

The invention discloses a method for efficiently separating and purifying mammary epithelial cells. The method is suitable for a plurality of mammals. The mammary tissues of adult mammals, which are in the middle and later periods of gestation or in the lactation period, are collected through aseptic operation. According to the provided method, the separation and purification can be achieved in one step, the whole experiment is simple and efficient; the purification, which comprises repeated differential digestion and differential attachment, in the conventional method is eliminated, the purity of mammary epithelial cells obtained by the provided method can reach 98% or more, the obtained mammary epithelial cells can be continuously cultured in-vitro and passed down for 40 generations, and the lactating function of mammary epithelial cells is not affected. The obtained mammary epithelial cells can be applied to the researches on growth of mammary gland, lactating mechanism, mammary bioreactor, transgenic animals, mammary diseases, and the like.

The invention relates to a separation and culture method for different cellular components of a human mammary tissue, and belongs to the technical field of cell culture. The method comprises the following steps: (1) sampling a fresh human mammary tissue specimen; (2) carrying out mechanical shearing and collagenase and hyaluronidase combined digestion to prepare a mammary tissue cell suspension; (3) carrying out low-speed and differential centrifugation to layer and separate the different cellular components; (4) carrying out centrifugation, washing and adherent culture for multiple times to purify the cell components, so as to obtain the different cellular components of the mammary tissue, which comprise epithelial cells, matrix cells and preadipocytes. The separation and culture method disclosed by the invention can be used for quickly, simply and effectively separating and purifying the different cellular components from the same mammary tissue; the cultured mammary gland cells are sufficient in quantity, good in cell viability and purity which is up to 95% above; the separation and culture method can provide very useful materials for molecular biology study associated with mammary glands and breast cancer, and establishes a foundation for construction of a mammary gland microenvironment multi-cell culture model.

The invention provides novel compositions, methods and uses, for the prediction, diagnosis, prognosis, prevention and treatment of malignant neoplasia and breast cancer. The invention further relates to genes that are differentially expressed in breast tissue of breast cancer patients versus those of normal “healthy” tissue. Differentially expressed genes for the identification of patients which are likely to respond to chemotherapy are also provided.

The invention discloses a mammary gland compression panel structure and a mammary gland detecting instrument. The mammary gland compression panel structure is used for being matched with a flat panel detector to detect mammary tissues in an assistant manner. The mammary gland compression panel structure comprises a compression panel and a connection component, wherein the compression panel is used for compressing the mammary tissues; the connection component is connected with the compression panel; in the process that the mammary gland compression panel structure is controlled to be pressed down, the compression panel is subjected to the reacting forces F of the mammary tissues and part of the reacting forces F act on the connection component so that the connection component is linked with the compression panel to move toward the direction far away from patients; the mammary tissues move along with the compression panel. The mammary gland compression panel structure has the beneficial effects that the mammary tissues move toward the direction far away from the patients along with the compression panel structure by moving the compression panel structure in the compression process, thus improving the compression effects, bringing the roots of the mammary tissues into the imaging range, reducing omission of foci and avoiding harms of the mammary tissues and the compression panel due to relative movement between the mammary tissues and the compression panel.

The invention relates to a sichuan lovage rhizome fresh medicine extract composition capable of diminishing swelling and stopping pain of breast. Extract components of all the fresh Chinese herbal medicines are combined, directly act on mammary tissues and are fully absorbed by the mammary tissues and breast lobule tissues, so that functions of the fresh medicine extract composition is better played, and the effects of alleviating manic-depressive spirit, preventing and treating breast lobular hyperplasia, mammary gland fibroma and enlargement of axillary lymph nodes as well as shrinking mammaaccessoria are realized; and skin is also effectively brightened, so that breast regains elasticity. Effective components in the fresh medicine oil for diminishing swelling and stopping pain of breast can be rapidly permeated into breast skin cells under the action of caprylic / capric triglyceride and ethylhexyl palmitate, the fast absorption of the fresh medicine extract composition and grape seed oil are helped, and excellent lubricating property of jojoba oil also enables usability of the fresh medicine oil to be better. The preparation method of the fresh medicine oil for diminishing swelling and stopping pain of breast has the advantages that the fresh medicine oil is homogenized and mixed, all the components effectively coordinate with each other, and the using effect is the best.