10 results about "Cardiolipin" patented technology

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.

The invention relates to the technical field of cosmetics, in particular to a moisturizing mask with a repairing effect and a preparation method of the moisturizing mask, and mask liquid comprises a sensitive skin repairing composition, an auxiliary effect system, a thickening system, a moisturizing system, a grease system, a buffer system and purified water. The sensitive skin repairing composition is prepared from chamomile oil, tea tree oil, a radix gentianae extract, a herba centellae extract, 4-tert-butyl cyclohexanol, butanediol, phosphatidyl ethanolamine and cardiolipin, phosphatidyl ethanolamine is modified, and the weight ratio of all the components is adjusted, so that the sensitive skin repairing composition is prepared. The traditional Chinese medicine composition has the advantages that various components in the composition are coordinated to play the optimal anti-inflammatory, anti-allergy, analgesic and repairing functions, exogenous stimulation, nerve stress response and inflammatory response are reduced, the self-protection mechanism is strengthened, the skin barrier is repaired, the oxidative stress capability is further strengthened, and the composition has more excellent high-temperature and low-temperature stability; the traditional Chinese medicine composition is natural and safe, and does not generate side effects such as dependence and drug resistance after long-term use.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belongs to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The application belongs to the field of biological medicine, and relates to a drug-loaded nanovesicle and a preparation method and application thereof. The drug-loaded nanovesicle comprises: a vesicle core comprising siRNA capable of specifically targeting a silenced NR1D1 gene; and a vesicle membrane fused by red blood cell membranes, macrophage membranes, cardiolipin, cholesterol and lecithin. The drug-loaded nanovesicle can specifically target macrophages in the immune suppression stage of sepsis, and has an intracellular response release function. By inhibiting the expression of NR1D1, the drug-loaded nanovesicle restores the functions of BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axes, reestablishes the phagocytosis function of macrophages and the lysosome-dependent bacterial clearance capacity, significantly improves the survival rate of animals in a sepsis immune suppression model and reduces the bacterial load. Compared with a traditional electroporation method, the preparation method of the application significantly improves the encapsulation rate of siRNA.

The invention relates to the technical field of cosmetics, in particular to a sensitive skin repairing composition and application thereof.The mask liquid is prepared from chamomile oil, tea tree oil, radix gentianae extract, centella asiatica extract, 4-tert-butyl cyclohexanol, butanediol, phosphatidyl ethanolamine and cardiolipin. Phosphatidyl ethanolamine ester is modified, the weight ratio of all the components is adjusted, all the components in the composition are coordinated to play the optimal functions of diminishing inflammation, resisting allergy, easing pain and repairing, exogenous stimulation, nerve stress response and inflammatory response are reduced, meanwhile, the self-protection mechanism is strengthened, the skin barrier is repaired, and the skin repairing effect is achieved. The composition disclosed by the invention is free from preservatives, natural and safe, so that the product is milder and does not generate side effects such as dependence and drug resistance after being used for a long time.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The present invention relates to a novel substituted acridinium salts as fluorescent dyes, as well as methods of their manufacturing and use of the disclosed compounds for the detection of cardiolipin.

The invention relates to a method for regulating and controlling a cardiolipin-containing membrane structure by utilizing mid-infrared light and application, and belongs to the field of biophysical regulation and control. The method comprises the following steps: applying a mid-infrared electric field with specific frequency and intensity to the mitochondrial-like phospholipid bilayer membrane system containing cardiolipin, and triggering the change of membrane structure parameters and cardiolipin behaviors of the mitochondrial-like phospholipid bilayer membrane system. The method comprises the following steps: constructing a mitochondrial membrane model through CHARMM-GUI, applying alternating electric fields with different frequencies and intensities by using a GROMACS platform, analyzing by combining indexes such as membrane thickness, cardiolipin orientation angle and cardiolipin density distribution, and determining 53.04 THz and 53.16 THz as characteristic frequencies of cardiolipin; 0.7 V / nm is determined as the optimal intensity. The mid-infrared light non-contact operation is adopted, the method has the advantages of being high in frequency selectivity and reversible in regulation and control, moderate change of the membrane structure can be caused under the specific frequency and proper electric field intensity, and a theoretical basis is provided for light regulation and control of the mitochondrial function, drug delivery and disease intervention.

The application relates to the technical field of medicines, and discloses a polymer with pyroptosis execution protein function and a preparation method and application thereof. The polymer has the structure shown in the description, wherein R1 is as shown in the description, R2=-CO(CH2) n CH3.In the application, EPL is taken as a skeleton, a structural unit capable of specifically combining with an anionic lipid is grafted, and a fatty chain is obtained, the polymer can quickly break the inner side of a cell membrane, induce cell blebbing, release inflammatory factors, play a similar GSDM function, make cells die in a similar pyroptosis mode, exhibit excellent anti-tumor effects, and can combine with an anionic lipid cardiolipin on the outer side of a bacterial membrane, so that the bacterial membrane is broken, and the bacteria can be killed in a short time, and excellent antibacterial treatment effects are achieved. The polymer has strong scientific research value and good clinical transformation prospects.