42 results about "Macrophage phagocytosis" patented technology

The invention discloses a clam bioactive peptide and its extraction method and use. The clam bioactive peptide has an N-tail end sequence of TRGIDEIQNT GGGGTRR, an isoelectric point of 8.79 and molecular weight of 18KDa. The clam bioactive peptide can promote macrophage phagocytosis activity and splenic lymphocyte propagation capability of an immunization system, has pharmacological effects of improving immunity, can be used for preparation of a drug or a health food for improving immunity and has potential of exploitation of novel immunopotentiation drugs.

The invention relates to 6 saturated fatty chain alcohol Glu-Asp-Gly tripeptide ester conjugates with immunosuppressive activity in a general formula Glu-Asp-Gly-O-CH2-(CH2)nCH3 I. In saturated fatty chain alcohol, n is equal to 6, 8, 10, 12, 14 or 16. The invention also relates to a preparation method and application of the conjugates as an immunosuppressant. An experimental result of the saturated fatty chain alcohol Glu-Asp-Gly tripeptide ester has the inhibition effects of splenic lymphocyte mitogen breeder reaction and macrophage phagocytosis activity indicates that the conjugates of the invention have excellent immunosuppressive action and can be clinically used as an immunosuppressant.

The invention relates to 6 saturated fatty chain acid Glu-Asp-Gly tripeptide amide conjugates with immunosuppressive activity in a general formula CH3 (CH2)n-CO-Glu-Asp-Gly I. In saturated fatty chain acid, n is equal to 6, 8, 10, 12, 14 or 16. The invention also relates to a preparation method and application of the conjugates as an immunosuppressant. An experimental result of the saturated fatty chain acid Glu-Asp-Gly tripeptide ester which has the inhibition effects of splenic lymphocyte mitogen breeder reaction and macrophage phagocytosis activity indicates that the conjugates of the invention have excellent immunosuppressive action and can be clinically used as an immunosuppressant.

The invention relates to the technical field of medicine, and provides new application of macrophage beta-profilin-1, namely application of macrophage beta-profilin-1 to preparation of a drug for preventing or treating atherosclerosis. It is proved through experiments that in comparison with wild type mice, the serum total cholesterol content of macrophage beta-arrestin-1 specificity myelogenous knockout mice is remarkably increased, and it is found through oil red O dyeing that the aorta overall plaque area and the plaque area of aorta roots are obviously increased compared with those of the wild type mice. By changing the macrophage beta-arrestin-1 gene expression level through virus transfection, and it is found that macrophage phagocytosis lipid is increased by interfering beta-arrestin-1 expression and is decreased by overexpressing beta-arrestin-1. The results show that the beta-arrestin-1 gene has a protective effect on the severity degree and prognosis of atherosclerosis.

The invention relates to 12 saturated fat chain acid His-Gly-AA tripeptide amide conjugates having immunosuppressive activity in a general formula I: CH3(CH2)n-CO-His-Gly-AA, wherein AA is Glu or Lys; and n in the saturated fat chain acid is equal to 6, 8, 10, 12, 14 or 16. The invention relates to a preparation method for the tripeptide amide conjugates and use of the tripeptide amide conjugates as immunosuppressive agents. Experimental results that the saturated fat chain acid His-Gly-AA tripeptide amide has the effects of inhibiting splenic lymphocyte cell mitogen breeder reaction and inhibiting macrophage phagocytosis activities show that the compounds have good immunodepression effect and can be clinically used as the immunosuppressive agents.

The invention belongs to the field of traditional Chinese medicines and particularly relates to a traditional Chinese medicine composition having an immunity boosting function, a preparing method thereof and uses of the composition. Channel tropisms, properties and flavors of traditional Chinese medicines comprising ginseng, Chinese magnolia vine fruit, dwarf lilyturf tuber, barbary wolfberry fruit and danshen root are fully considered in the composition, and a ratio of the traditional Chinese medicines is selected to allow the traditional Chinese medicines to be matched and cooperated according to a "monarch, minister, assistant and guide" compatibility relation, thus allowing the composition to be acted on focuses and fully exerting the immunity boosting function. Experiments prove that the composition can increase the serum murammidase level of mice, enhances in-vitro macrophage phagocytosis functions of the mice to a certain degree, and can be adopted as a potential immunity boosting medicine.

The invention discloses traditional Chinese medicine with an immunity enhancing function. The traditional Chinese medicine is prepared from five types of Chinese medicinal materials, namely, Chinese yam, barbary wolfberry fruit, Chinese angelica, Chinese date and donkey-hide gelatin. As proven by pharmacodynamic test, the traditional Chinese medicine has the effects of remarkably raising the serum hemolysin titer level of a mouse, improving the macrophage phagocytosis capability of the mouse and promoting the NK cell activity of the mouse, and can be used for improving the immunologic function of the mouse. As proven by safety test, the formula is safer than brain-refreshing tea, is more scientific, and is more suitable for long-time taking.

The invention relates to an amino acid sequence for inhibiting PTX3 to treat nasopharyngeal carcinoma. It can be used to inhibit PTX3 from promoting the migration and invasion of nasopharyngeal carcinoma cells, promoting angiogenesis and inhibiting macrophage phagocytosis to further treat nasopharyngeal carcinoma.

The invention relates to panax ginseng glycopeptide as well as a preparation method and medical application thereof, and belongs to the field of medicines. Panax ginseng is used as a raw material, and panax ginseng glycopeptide is obtained through the steps of extraction through a decoction method, separation through an ethanol precipitation method, hydrolysis through a double-enzyme enzymolysis method, dialysis, freeze drying and the like, wherein the yield of the panax ginseng glycopeptide is 14% or above, and the purity is 90% or above. Animal model experiments are carried out, and results show that the panax ginseng glycopeptide can enhance the cellular immune function, humoral immune function, mononuclear-macrophage phagocytosis function and NK cell activity of immunocompromised mice. The panax ginseng glycopeptide is novel in preparation method, relatively high in yield and purity and suitable for industrial production, serves as an active pharmaceutical ingredient, is expected to be developed into novel drugs for preventing and treating low-immunity diseases and auxiliary drugs for low-immunity diseases caused by tumors, cancers and the like, and has a wide application prospect. Clinical guidance and medication basis are provided for prevention and treatment of hypoimmunity diseases, so that the pain of patients is relieved, and the living quality of the patients is improved.

The invention relates to a long-circulating nanoparticle for targeting circulating tumor cells. The long-circulating nanoparticle is prepared from chemical medicine, a solid lipid material and an emulsifier, wherein the surface of the nanoparticle is modified with macrophage phagocytosis-resistant polypeptide and circulating tumor cell targeting polypeptide. The invention also provides a preparation method of the long-circulating nanoparticle. The preparation method comprises the following steps of mixing the macrophage phagocytosis-resistant polypeptide, the circulating tumor cell targeting polypeptide, and an organic solvent dissolved with a chemical drug, and then mixing the melted solid lipid material, so as to obtain an oil phase; uniformly dispersing the emulsifier into water, so as to obtain a water phase; adding the oil phase into the water phase, and removing the organic solvent after the dispersing is uniform. The invention also provides application of the long-circulating nanoparticle in preparing a preparation for treating the circulating tumor cells. The long-circulating nanoparticle for targeting the circulating tumor cells has the advantages that the circulating tumor cells can be identified in the blood circulation in a targeting way, the phagocytosis of the macrophage is inhibited, the withering of the circulating tumor cells is caused by the chemical medicine, the tumor transfer process is disturbed, and the tumor transfer is obviously prohibited.

The invention relates to a cis-platinum microparticle system composition which is lower in cost but has higher cis-platinum drug loading capacity and a macrophage phagocytosis shielding effect, and apreparation method. Cis-platinum nanoparticles prepared from a methoxy polyethylene glycol 2000-sodium polyglutamate copolymer (MPEG2000-P(GluNa)2000) as a carrier and cis-platinum under the preparation method conditions required by the patent have higher drug loading capacity, can better shield phagocytosis of macrophages in an in-vivo blood circulation process, and have better tumor tissue distribution.

The invention relates to 12 kinds of saturated fatty chain amine His-Gly-AA tripeptide amide conjugates with immunosuppressive activity in a general formula His-Gly-AA-NH-CH2-(CH2)nCH3 I, wherein AA stands for Glu or Lys, and n is equal to 6, 8 ,10, 12, 14 or 16 in the saturated fatty chain amine. The invention also relates to a preparation method and the application of the conjugates as an immunosuppressant. An experimental result of the saturated fatty chain amine His-Gly-AA tripeptide amide for the inhibition effects of splenic lymphocyte mitogen proliferation reaction and macrophage phagocytosis activity indicates that the conjugates of the invention have excellent immunosuppressive action and can be clinically used as an immunosuppressant.

The present invention relates to application of cordyceps sinensis mycelium solid fermentation water extract in preparation of products with immune regulation functions. The cordyceps sinensis mycelium solid fermentation water extract of the invention is prepared by using wild cordyceps sinensis (Berk.) sporocarp through the steps of strain collection, preservation and rejuvenation, preparation of parent strain, first-grade liquid strain and second-grade liquid strain, preparation of solid fermentation objects, and water extraction. The obtained cordyceps sinensis mycelium solid fermentation water extract is used for conducting immune regulation experiments for mice. Experiment results show that the cordyceps sinensis mycelium solid-state fermentation water extract can substantially enhance macrophage phagocytosis of mice with low immune function, can adjust the phagocytosis rate of the mice with low immune function to a level approaching normal, has immune regulation function and can be used for preparing products with immune regulation functions.

The invention discloses a system for characterizing macrophage phagocytosis microorganisms of pompanos. The pompanos are dissected and separated through a surgical knife and tweezers to obtain heads and kidneys; the heads and the kidneys are abraded through a cell sieve to obtain cell suspension containing macrophages, and the macrophages of the heads and the kidneys are obtained through a Percoll solution density gradient centrifugal tube. The survival amount of cells cultured in vitro through a trypan blue dyeing manner is counted, and the survival condition in one week is detected. The system is used for carrying out a phagocytosis experiment, and phagocytosis conditions of nocardia, yeast and the like of the pompanos by the head and kidney macrophages of trachinotus ovatus are determined, and a phagocytosis function is observed. An experiment result of the system shows that the macrophages of the pompanos can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the pompanos can be observed very well. The system disclosed by the invention lays a foundation for further researches on physiology, pathology and toxicology of the macrophages of the pompanos.

The invention discloses a pharmaceutical composition of emetine hydrochloride and application of the pharmaceutical composition in biomedicine. The pharmaceutical composition comprises the emetine hydrochloride and a natural product compound (I) of a novel structure; when in single action, the emetine hydrochloride and the natural product compound can enhance the pulmonary macrophage phagocytosis function and AMphi activity, and play a role in improvement of the pulmonary immunization function; when the emetine hydrochloride and the natural product compound are in combined action, the improvement effect in the pulmonary immunization function is further improved, and the pharmaceutical composition can be developed into drugs for improving the pulmonary immunization function; compared with the prior art, the pharmaceutical composition has outstanding substantial characteristics and significant improvement.

The invention discloses a throat-clearing pharynx-moistening composition, which is prepared by mixing the following components: haematococcus pluvialis astaxanthin microcapsule powder or water-solublehaematococcus pluvialis sporoderm-broken spore powder, a fructus momordicae extract, a flos lonicerae extract, a flos chrysanthemi extract, a radix scutellariae extract, a radix platycodon extract, aherba rabdosiae extract, a semen sterculiae lychnophorae extract, a fructus canarli extract, xanthan gum, locust bean gum, an edible essence and a preservative. The throat-clearing pharynx-moisteningcomposition provided by the invention has the efficacies of resisting oxidation, resisting bacteria, resisting virus, diminishing inflammation, relieving cough and eliminating phlegm, can be beneficial to improving macrophage phagocytosis and neutrophil sterilizing power, improving lysozyme activity and enhancing organism immunity, is natural without toxic and side effects, can be taken for a long term so as to achieve the effects of curing or preventing pharyngitis, and is simple in components and suitable for mass production.

The invention discloses a high-bonding-strength coating based on ion slow release and shape retention design and a preparation method and application thereof, and belongs to the field of titanium-based surface treatment technology and biomedical application, and the mechanical embedding force of the coating and a substrate is increased through acid etching treatment to achieve high bonding strength; meanwhile, according to the characteristics of the crystal structure of the titanate, on the premise that the configuration of the titanate is basically unchanged, through ion exchange doping and Co < 2 + > release, and in combination with the nanorod topological configuration of the bone-imitating matrix, triple functions of inducing phagocytosis of macrophages to remove bacteria, promoting angiogenesis and promoting osteogenic differentiation of mesenchymal stem cells can be realized. The coating is simple in preparation method and wide in raw material source, and has a certain application prospect.

An anti-CD47 monoclonal antibody and use thereof. The provided anti-CD47 monoclonal antibody can effectively inhibit tumor growth. Blocking human SIRP and human CD47 signals may enhance macrophage phagocytosis of tumor cells, prevent the tumor cells from escaping a tumor immune defense system, and have an anti-tumor function. Blocking association between the CD47 on a tumor cell surface and the SIRP on a macrophage surface may block a “do not eat me” signal from the tumor cells, promoting tumor cell recognition and uptake of macrophages, and thereby facilitating tumor cells to be phagocytosed. The association between the CD47 on a tumor cell surface and the SIRP on a macrophage surface is a common “do not eat me” signal. The anti-CD47 antibody, as a very promising target in the tumor immune system, will play a powerful and effective role in human cancer therapy.