The invention relates to theanine-modified carboline acyl amino acid benzyl ester, as well as preparation, anti-tumor activity and application thereof, discloses 17 compounds represented by a general formula I (in the formula, AA is selected from L-Leu, L-Glu, L- Val, L-Trp, L-Pro, L-Tyr, L-Met, L-Phe, L-Ala, L-Gly, L-Asn, L-Asp, L-Ile, L-Lys, L-Ser and L-Theanine residues, or AA-OBzl jointly represents OBzl), and discloses preparation and synthesis methods thereof. The invention further discloses in-vivo and in-vitro anti-tumor activity thereof and clinical application prospects thereof as anti-tumor medicaments.