The invention relates to an enveloped
microorganism in whose
genome the following components are inserted and can be expressed: I) a
nucleotide sequence that encodes a directly or indirectly, antiproliferatively or cytotoxically active expression product or a plurality of said expression products, II) a
nucleotide sequence that encodes or is
constitutively active for a
blood plasma protein under the control of a activation sequence that can be activated in the
microorganism, III) optionally, a
nucleotide sequence that encodes or is
constitutively active for a
cell-specific ligand under the control of an activation sequence that can be activated in the
microorganism, IV) a nucleotide sequence for a
transport system that induces expression of the expression products of components I) and II) and optionally III) on the outer surface of the microorganism or that induces
secretion of the expression products of component I) and expression of
component II) and optionally component III) and that is preferably
constitutively active, V) optionally a nucleotide sequence for a
protein used for
lysis of the microorganism in the
cytosol of mammalian cells and for the
intracellular release of plasmids with at least one or more components I) and VI) contained in the lysed microorganism, and VI) an activation sequence that can be activated in the microorganism, and / or that is tissue-specific, tumor
cell-specific, function-specific or not
cell-specific, for expressing component I). The inventive microorganism is further characterized in that any of components I) to VI) can be present either single or several times, and can be either identical or different.