35 results about "Axon growth" patented technology

A BoC includes one or more chambers configured to house a population of neurons to be cultured, and one or more cellular-communication platforms placed between at least one pair of chambers from the one or more chambers. The cellular- communication platforms include one or more features / structures configured, shaped, sized, and arranged to promote axonal growth of the population of neurons in a preferred direction and impede axonal growth in directions opposite to the preferred direction, thereby providing control over at least one of, directionality, transmittance, and / or lateral spread between the populations of neurons being cultured. The cellular-communication platforms generate a neuronal circuit between the population of neurons in each pair of the chambers.

The invention relates to the technical field of peripheral nerve injury suture, and particularly discloses a radio stimulation surgical suture for promoting peripheral nerve healing and a preparation method and application thereof, the radio stimulation surgical suture comprises a Mo core layer and a PLGA coating; the preparation method of the radio stimulation operation suture comprises the following steps: preparing a core layer by using metal Mo, spraying out a PLGA spinning solution through a spinning nozzle, curing in a coagulating bath to form a film, and forming a uniformly coated PLGA coating on the Mo core layer through a wet spinning technology to obtain the radio stimulation operation suture for promoting peripheral nerve healing, according to the prepared radio stimulation surgical suture, energy is obtained from an external electromagnetic field in an electromagnetic induction and capacitance coupling mode, and a stable electric field is formed on the suture and the local part of a wound, so that the electric field acts on damaged tissue. The suture can promote neuronal axon growth, Schwann cell migration and the like, and the nerve tissue regeneration efficiency is improved.

The invention relates to the technical field of biomedical materials, in particular to a high-conductivity peripheral nerve socketed pipe and a preparation method thereof. The nerve socketed pipe is a hollow cylindrical socketed pipe made of a material containing poly (octylene-citrate-co-octanol) POCO, and a regeneration promoting material is loaded in the hollow cylindrical socketed pipe; the regeneration promoting material comprises fetal calf serum and a DMEM cell culture medium. The POCO has the advantages of conductive activity, nerve regeneration induction, free radical scavenging ability, oxidation resistance, deformation resistance and the like, and has good degradability and biocompatibility, so that the nerve socketed pipe is prepared from poly (octylene-citrate-co-octanol), and the growth of axons is accelerated through electrical stimulation. Furthermore, at least one conductive monomer of pyrrole, aniline, p-phenylenediamine, o-methoxyaniline, thiophene, 3-hexylthiophene, 3-thiopheneacetic acid and 3, 4-ethylenedioxythiophene is adopted for modifying the POCO hollow cylindrical socketed pipe through in-situ oxidative polymerization, and the performance of the POCO hollow cylindrical socketed pipe serving as the neural restoration material is further improved.

The invention discloses an application of a CDKN1A interaction zinc finger protein 1 in neuronal axon regulation and control. The invention proposes and verifies that the CDKN1A interaction zinc finger protein 1 can effectively regulate and control the growth behavior of neuron axons for the first time, the growth of the neuron axons and the regeneration of damaged axons can be promoted by inhibiting the expression of the CDKN1A interaction zinc finger protein 1, and a new direction is provided for research and development of drugs for treating diseases caused by neurodegenerative diseases and nerve injuries.

The invention provides an application of guanine and isoguanine in preparation of a drug for treating or repairing peripheral nerve injury, relates to the technical field of biomedicine, and provides an application of guanine Guanine and / or isoguanine Isoguanine in preparation of a drug for treating or repairing peripheral nerve injury. The specific embodiment verifies that the growth of DRG neuron axons can be obviously promoted by adding guanine and isoguanine into DRG neuron resuspended in vitro, and guanine and isoguanine in the application can regulate the growth of the DRG neuron axons in the peripheral nerve injury and regeneration process; the important effects of Guanine and Isoguanine in the nerve injury repairing process can be better understood, and meanwhile, the effectiveness of the medicine for repairing or treating peripheral nerve injury is verified; and a new target spot is provided for treatment of related nervous system diseases.

The invention discloses application of a lamprey transcription factor MKX in preparation of a medicine for promoting spinal cord injury repair, and belongs to the technical field of biology. The lamprey MKX gene is found to have a spinal cord injury repairing function by taking a mouse as a model for the first time, and can promote nerve cell proliferation and axon growth. The invention provides a new application of the lamprey MKX gene in spinal cord injury repair treatment, and provides a new perspective for research and clinical treatment of human spinal cord injury.

The invention discloses a bioreactor for nerve cell culture, and relates to the technical field of cell culture, a metal ball falls down to drive a culture dish to deflect, so that an azimuth magnetic field is generated, multi-stage energy storage is performed on the metal ball, the metal ball is bounced off after the energy is stored to a certain degree, and the bounced-off metal ball falls down disorderly to drive the culture dish to deflect disorderly. Therefore, the magnetic field is generated disorderly, irregular magnetic field stimulation is generated on nerve cells in the culture dish, and on one hand, the basic problems of cell adherence, cell block mass precipitation, uniform distribution of nutrient substances and oxygen, removal of metabolic waste and the like are solved; on the other hand, a stirring structure is not arranged, so that the problem of damage to cells caused by traditional stirring is avoided; and finally, the nerve cells are stimulated by combining a magnetic field disordered triggering mode with disordered deflection of the culture dish, so that the initiative of cell movement such as nerve cell axon growth, synaptic remodeling, cell migration and the like is stimulated.

According to the method for differentiating the SH-SY5Y cells into the neuron-like cells, rapid proliferation of the cells is facilitated in a DMEM culture medium containing 10% of serum, a differentiation reagent EC23 is used when differentiation begins, a DMEM / F12 culture medium with the concentration of 5% of serum is replaced, cell differentiation is facilitated, and finally, a serum-free culture medium NBA containing a differentiation reagent BDNF is used for differentiating the cells into the neuron-like cells. Compared with the prior art, the neuron-like cells with high SH-SY5Y differentiation efficiency can be rapidly obtained, a network is formed between the improved neuron-like cells, the survival rate of the cells is also greatly improved, and the neuron-like cells can be better used for research of subsequent experiments.

The application provides application of guanine and isoguanine in preparation of drugs for treating or repairing peripheral nerve injury, and relates to the technical field of biomedicine, and the technical scheme is characterized in that the application provides application of guanine Guanine and / or isoguanine Isoguanine in preparation of drugs for treating or repairing peripheral nerve injury.In the application, it is verified through specific examples that adding guanine and isoguanine in resuspended DRG neurons in vitro can significantly promote the growth of DRG neuron axons, and in the application, guanine and isoguanine can regulate DRG neuron cell axon growth in the process of peripheral nerve injury and regeneration, which helps better understand the important role of Guanine and Isoguanine in the process of nerve injury repair, and meanwhile, the effectiveness of the drugs for repairing or treating peripheral nerve injury is verified; and a new target point is provided for treatment of related nervous system diseases.

The present invention relates to the technical field of peripheral nerve injury suturing, and specifically discloses a radio-stimulated surgical suture for promoting peripheral nerve healing, and its preparation method and application. The radio-stimulated surgical suture comprises a Mo core layer and a PLGA coating. The preparation method of the radio-stimulated surgical suture is as follows: using metallic Mo to form the core layer, ejecting a PLGA spinning solution through a spinneret, and curing it in a coagulation bath to form a film, and forming a uniformly coated PLGA coating on the Mo core layer by wet spinning technology to obtain a radio-stimulated surgical suture for promoting peripheral nerve healing. The radio-stimulated surgical suture prepared by the present invention obtains energy from an external electromagnetic field through electromagnetic induction and capacitive coupling, and forms a stable electric field locally between the suture and the wound, thereby acting on the damaged tissue. The suture can promote neuronal axon growth, Schwann cell migration, etc., and improve the efficiency of nerve tissue regeneration.

The invention discloses application of a deubiquitinating enzyme inhibitor and an OTUD7A-HINT1-mTOR axis in regeneration of a central nervous system, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to promote the regeneration of the central nervous system. The invention finds that the deubiquitinating enzyme inhibitor PR-619 can promote the growth of axons in neuron cultures and retina explants, protect the survival of RGCs and enhance the regeneration of axons after ONC. The PR-619 can also promote CNS regeneration by down-regulating expression of HINT1, HINT is one of main targets of the PR-619 and plays a role through an mTOR signal channel, and OTUD7A serves as an upstream regulation factor of the HINT1. The PR-619 can be used for treating the CNS injury, the OTUD7A-HINT1-mTOR axis can be used as a CNS injury treatment target, and the PR-619 and the OTUD7A-HINT1-mTOR axis can be combined for treating the CNS injury.

The invention discloses a three-layer nerve conduit based on cell-loaded near-field direct-writing printing and a preparation method and application thereof, and is characterized in that the inner layer of the three-layer nerve conduit is a cell-loaded ALG / GelMA / PEO hydrogel stent, the middle layer is a PVA / GelMA electrostatic spinning membrane, and the outer layer is a fused near-field direct-writing PCL stent; the preparation method comprises the following steps: preparing an ordered fiber membrane PVA / GelMA from a spinning solution through electrostatic spinning, adding the ordered fiber membrane PVA / GelMA into an acetone / glutaraldehyde solution to obtain a PVA / GelMA electrostatic spinning membrane, and printing a direct-writing PCL support on any single surface of the electrostatic spinning membrane through fusion near-field direct-writing. The preparation method comprises the following steps: preparing an ordered cell-loaded hydrogel scaffold from cell-containing bio-ink through cell-loaded near-field direct writing printing, and finally preparing the three-layer nerve conduit, and the three-layer nerve conduit has the advantages of accelerating axon growth, promoting myelin sheath regeneration and improving nerve function recovery.

Preparation method and application of diterpenoid, relate to the application technical field of diterpenoid.The present application separates two new compounds of diterpenoid A and diterpenoid B from a strain of Hericium fermentation extract, and discloses chemical structural formula of the two compounds, and corresponding nuclear magnetic, mass spectrum, infrared and ultraviolet data, and its function of significantly inhibiting the production of inflammatory BV2 cell NO.Diterpenoid B has 24% of the promotion rate of axon growth of PC-12 cell, and simultaneously has significant inhibition of TLR4 / NF-κB signal path to inhibit downstream inflammatory proteins of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and diterpenoid A and diterpenoid B both show significant anti-neuroinflammatory activity.The present application can obtain the preparation method and application of diterpenoid.

A culture device for preparing separated assembloid comprises a first culture chamber, a second culture chamber and at least one assembloid culture unit, wherein the assembloid culture unit at least comprises a first cell culture cavity, a second cell culture cavity and one or more directional channels, wherein the first cell culture cavity and the second cell culture cavity are configured to culture a first cell and a second cell, respectively; the directional channels are filled with a directional induction growth culture medium to allow the first cell and / or the second cell to undergo directional axon-induced growth in the directional induction growth culture medium; and the first culture cavity and the second culture cavity are respectively configured to contain a first culture medium suitable for the growth of the first cell and a second culture medium suitable for the growth of the second cell, neither of which is mixed with the other. The assembloid built by the culture device can not only be used for basic research on the growth, projection, and synaptogenesis of neuron axons from a human source, but also provides a research model for human neurological diseases, and neuronal axon regeneration and myelination.

The invention discloses a culture device for preparing separated combined organoid. The culture device comprises a first culture chamber, a second culture chamber and at least one combined organoid culture unit, wherein the combined organ culture unit at least comprises a first cell culture cavity, a second cell culture cavity and one or more directional channels; wherein the first cell culture cavity and the second cell culture cavity are configured to culture first cells and second cells; the directional channel is filled with a directional induced growth culture medium, so that the first cells and / or the second cells are allowed to generate directional axon induced growth in the directional induced growth culture medium; the first culture cavity and the second culture cavity are respectively configured to contain a first culture medium suitable for growth of first cells and a second culture medium suitable for growth of second cells, and the first culture medium and the second culture medium are not mixed. The combined organ built by utilizing the culture device disclosed by the invention not only can be used for carrying out fundamental research on growth, projection and synaptic formation of various human neurons, but also can provide a research model for human nerve diseases and neuronal axon regeneration and myelination.

The present invention belongs to the technical field of preparation of anti-tumor drugs, and more specifically, relates to a drug-loaded bacterial outer membrane vesicle that targets and intervenes in the tumor neural microenvironment and a preparation method thereof. The drug-loaded bacterial outer membrane vesicle includes a bacterial outer membrane vesicle, and a small molecule drug with a neuroinhibitory effect wrapped by the bacterial outer membrane vesicle, and the surface of the bacterial outer membrane vesicle is also modified with a nerve cell targeting molecule. The drug-loaded bacterial outer membrane vesicle provided by the present invention can effectively target nerves in the tumor microenvironment, while effectively blocking the interaction between nerves and tumors, inhibiting nerve infiltration and nerve axon growth in the tumor microenvironment, and thus inhibiting tumor growth. The preparation method of the present invention is simple and highly operable, and the prepared drug-loaded bacterial outer membrane vesicle has good biocompatibility and good clinical application prospects.

The present invention relates to use of slow-releasing hydrogen sulfide organic donor ADT-OH drugs in the differentiation of neural precursor cells. ADT-OH can induce more directed differentiation of neural precursor cells into neurons and oligodendrocytes and less differentiation into astrocytes, promote the axon growth of neurons, and reduce the death of neural precursor cells, thereby providing a direction for the transplantation of neural precursor cells to repair damaged nerves. ADT-OH will likely become a new drug target for the clinical treatment of nervous system diseases.

The application discloses a nerve regeneration guide conduit for promoting nerve cell differentiation and a preparation method and application thereof. The nerve regeneration guide conduit can realize stable and long-acting release of bioactive factors, effectively reduce the risk of tissue adhesion, efficiently induce nerve cell differentiation, promote axon growth and function recovery, and provides a novel and effective solution for nerve regeneration and repair. In addition, the nerve regeneration guide conduit has excellent conductivity, biocompatibility, mechanical adaptability and the like, and therefore has a wide application prospect in the field of nerve regeneration and repair.

The invention relates to a neural stem cell-parathymosin composite cell preparation and application thereof in treatment of nerve injury diseases. According to the invention, it is found for the first time that neural stem cells overexpressing parathymosin (PTMS) can effectively promote neuron neogenesis and axon growth in in-vitro and in-vivo experiments; animal experiment results show that the traditional Chinese medicine composition can also be used as a treatment means for promoting nerve regeneration to resist dyskinesia related to nerve injury diseases.

The invention provides application of Syn3 in preparation of medicines for treating spinal cord injury, and belongs to the technical field of biological medicines. The invention finds that the PSD95 binding peptide Syn3 can promote coupling of rkB-PSD95-G alphai1 / 3 signal compounds, promote a BDNF-TrkB downstream PI3K-Akt signal channel and play a role in promoting neuronal axon growth, meanwhile, the Syn3 can penetrate through a blood spinal cord barrier to reach a spinal cord part and be stably combined with PSD95, after in-vivo injection, growth cone depolymerization and axon regeneration after spinal cord injury can be promoted, a neural circuit is further reconstructed, and the effect of promoting neuronal axon growth is achieved. Motion functions and nerve electrophysiology of the hind limbs of the mouse are recovered.

The invention relates to the technical field of microbial medicines, and particularly discloses application of intestinal golden thread bacteria in preparation of a medicine for preventing or treating peripheral nerve injury. A mouse model proves that the intestinal golden thread bacteria can regulate the injury microenvironment in the peripheral nerve injury repair process, guide nerve axons to extend in the expected direction and reduce uncontrolled axon growth and wrong dominance, so that the structural accuracy of nerve regeneration is improved.

The invention relates to the technical field of medical equipment, and discloses an electrical stimulation device for axon regeneration and a control method. The electrical stimulation device comprises a control assembly used for generating an electrical signal and transmitting the electrical signal to a stimulation electrode; the stimulation electrodes are connected with the control assembly and are used for being placed on the two sides of a nerve injury plane respectively, so that an electric field is formed in the axon growth direction based on the electric signals, and axons are stimulated to grow across the nerve injury plane; the axon growth stimulation method is simple, and the cost is controllable; and the direction of the electric field is matched with the growth direction of the axons, so that the problem of disorder of a nerve regeneration network caused by random growth of the axons possibly caused by a traditional treatment method can be avoided, and the axon growth effect is improved.

The invention discloses application of the M2 type microglial cell exosome. The invention discloses an application of an M2 type microglial cell exosome in preparation of a product for treating diabetic retina neuropathy. In combination with a specific embodiment, the M2 type microglial cell exosome (M2-Exos) is separated and proved to promote the survival and axon growth of primary RGCs in vitro. In an STZ-induced diabetic mouse model, in-vivo injection of M2-Exos prevents RGC loss by inhibiting a pro-inflammatory mediator and enhancing an anti-inflammatory factor, so that ganglion cell complex thickness and visual ability are retained. The axon regeneration promoting ability of the M2-Exos is further verified in an optic nerve crushing animal model. The discovery shows that the M2-Exos is a potential neuroprotection and axonal regeneration promotion tool for treating the diabetic retina neuropathy, and the M2-Exos can be used for preparing products for treating the diabetic retina neuropathy.

The invention discloses a Wipi1 gene inhibitor and an application thereof. The Wipi1 gene inhibitor comprises siRNA used for inhibiting Wipi1 gene expression, and the Wipi1 gene inhibitor can be applied to preparation of drugs for repairing nerve injury or drugs for promoting neuronal axon growth. The neuronal axon growth is promoted by down-regulating the expression quantity of the Wipi1 gene in the neurons, and the neuronal axon growth is regulated in vitro by taking the Wipi1 gene as a target spot. According to the invention, the Wipi1 gene is used as a regulation target to regulate the regeneration of neuronal axons after peripheral and central nervous injury; regeneration and repair after neuronal axon injury are promoted by down-regulating expression of the Wipi1 gene, a brand new regulation target is provided for treatment of neuronal axon injury, and development of new treatment means and strategies is facilitated.

The present invention discloses a three-layer nerve conduit based on cell-loaded near-field direct writing printing, a preparation method thereof, and an application thereof. The three-layer nerve conduit is characterized in that the inner layer of the three-layer nerve conduit is a cell-loaded ALG / GelMA / PEO hydrogel scaffold, the middle layer is a PVA / GelMA electrospun membrane, and the outer layer is a melt near-field direct writing PCL scaffold. The preparation method comprises the steps of preparing an ordered fiber membrane PVA / GelMA by electrospinning a spinning solution, adding an acetone / glutaraldehyde solution to the ordered fiber membrane PVA / GelMA to obtain a PVA / GelMA electrospun membrane, printing a direct writing PCL scaffold on any single side of the electrospun membrane by melt near-field direct writing, preparing a cell-containing bio-ink into an ordered cell-loaded hydrogel scaffold by cell-loaded near-field direct writing printing, and finally preparing the three-layer nerve conduit. The three-layer nerve conduit has the advantages of being able to accelerate axonal growth, promote myelin regeneration, and improve nerve function recovery.

A cell matrix nerve graft for repairing peripheral nerve injury and a preparation method thereof are provided. The cell matrix nerve graft comprises acellular matrix and a scaffold, wherein the acellular matrix is obtained by decellularization after secretion and formation of stem cells, and the acellular matrix is wrapped around the scaffold. The defects of autologous nerve transplantation repair can be overcome and the immunogenicity of allogeneic cell transplantation repair is avoided. The cell matrix nerve graft beneficial to nerve cell proliferation and migration is provided, a microenvironment beneficial to axon growth is established, the purposes of nerve regeneration and function repair are achieved, and a feasible solution is provided for clinical treatment of nerve defects.

The invention provides methods of promoting retinal ganglion axon formation, survival, and synapse formation.

The invention provides an application of an inhibitor containing 2 (dybindin domain containing 2, Dbndd2) in a small muscle nutritional protein domain in preparation of a medicine for promoting nerve growth, and a preparation method of the inhibitor containing 2 (dybindin domain containing 2, Dbndd2). The invention discloses that knock-down of Dbndd2 gene expression can promote neuronal axon growth and damaged nerve regeneration for the first time, which indicates that Dbndd2 can be used as a drug design target, and a Dbndd2 inhibitor can promote neuronal axon growth and is used for treating traumatic nerve injury and neurodegenerative diseases related to nerve injury.

The present disclosure provides a method of treating or ameliorating a disease or disorder comprising administering to a subject a crystalline form of a composition comprising crystalline forms A, B, and C of psilocin and psilocybin. The disclosure further provides a method of stimulating neurogenesis or neurite growth resulting in an increase in neuronal length.