41 results about "Self-assembling peptide" patented technology

Embodiments are directed to a UPEC conjugate peptide including a self-assembling peptide and at least one UPEC epitope. The UPEC conjugate peptide may self-assemble into a nanofiber or fibril. Compositions including the UPEC conjugate peptide may be used to treat a bacterial infection such as a urinary tract infection (UTI). The compositions and methods may be specific for pathogenic bacteria. The compositions and methods may treat the infection without altering the gut microbiome.

The application discloses a kind of β-hairpin structure self-assembly capture antibacterial peptide J-1-RF and its application, it is related to biological medicine technical field.The amino acid sequence of the β-hairpin structure self-assembly capture antibacterial peptide J-1-RF is as shown in SEQ ID NO.4.The application selects the broad-spectrum antibacterial short peptide J-1 derived from honeybee royal jelly as template peptide, designs and transforms according to the amino acid sequence characteristics of short peptide J-1, obtains hydrophilic and hydrophobic amino acid alternately arranged structure unit, and uses it as self-assembly peptide fiber skeleton. D PG and RRRF two turning sequences are used for β-hairpin peptide design, so that it has the dual effect of antibacterial and bacterial capture.The β-hairpin structure self-assembly capture antibacterial peptide J-1-RF provided by the application can form fiber network structure, has good bacteriostatic effect and high stability, and has excellent biocompatibility, which provides effective technical support for developing new antibacterial drugs.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The present invention provides a material or method that can replace conventional materials and methods for preventing digestive fluid leakage. The digestive fluid leakage preventing material of the present invention comprises a self-assembling peptide gel, has an adhesion retention rate of at least 40% on a collagen sheet, and a decomposition rate of 35% or less based on pancreatic juice treatment at 37°C for 7 days.

[Problem] The present invention provides a hemostatic composition and a hemostatic method using the hemostatic composition, and specifically provides a composition for hemostasis that can be uniformly applied to a bleeding site and exert a high hemostatic effect. [Solution] A composition to be applied as a spray to a subject is provided, wherein the composition is characterized in that the spray is for hemostasis, and the composition comprises a self-assembling peptide that gels due to self-assembly when the composition is applied to a bleeding site of the subject, and the self-assembling peptide is contained in the composition at a concentration with an improved hemostatic ability compared to direct application.

The present disclosure describes a peptide scaffold for producing vaccines. The peptide scaffold includes a peptide that self-assembles into a hapten carrier (hC) includes amphipathic alpha-helices. The peptide includes heptad repeats following a specific pattern. The hC further includes hapten or an agent conjugated to it, and optionally the hC includes one or more T-cell epitopes at the N- and / or C-terminus of the one or more amphipathic alpha-helices. The present disclosure also describes compositions including immunogenic compositions including the hapten-hC or agent-hC conjugate.

Compositions containing self-assembling peptides and / or self-assembling peptidomimetics (“self-assembling peptides”) can be used to create a long-lasting “lift” or means of elevating tissue to be resected, dissected, manipulated or repaired, as a bulking agent, or as a tissue forming matrix by injection of a solution that forms a solid gel in situ, which is stable for a prolonged period of time from days to a month, is hemostatic, and may prevent adhesions. These self-assembling peptides and methods of use thereof enable better separation of tissues and visualization of margins, more durable and robust lifts, less need for frequent injections that carry risk of undesired perforation, and simultaneous management of adverse effects, such as bleeding, leaking, inflammation and iatrogenic injury during endoscopic, laparoscopic or other minimally invasive, or open surgical procedures in and / or on the body.

The present invention provides a self-assembling peptide gel for use in preventing a leak of digestive fluid and a self-assembling peptide gel for use in protecting an organ against digestion by a digestive fluid. The self-assembling peptide gel comprises a self-assembling peptide and water, whereby the self-assembling peptide gel has an adhesion persistence ratio with respect to a collagen sheet of at least 40% and a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days, and the self-assembling peptide gel is positively charged as a whole.

Disclosed herein is a versatile injectable nano-bioactive scaffold designed to achieve both immediate soft tissue volumization and long-term tissue regeneration. By utilizing biocompatible polymers such as hyaluronic acid, chitosan, and alginate, along with self-assembling peptides and nano-structured carriers including mesoporous silica nanoparticles and liposomes, the system facilitates controlled release of essential growth factors. This innovative approach delivers sustained bioactivity, significantly reducing the need for repeated injections and minimizing inflammatory complications. The system promises superior clinical outcomes, advancing the field of tissue engineering and regenerative medicine by providing an effective and lasting solution for tissue repair and regeneration.

The invention discloses self-assembled super-elastic protein, hydrogel and application of the self-assembled super-elastic protein and the hydrogel, and belongs to the technical field of biology. The self-assembled super-elastic protein provided by the invention comprises a self-assembled peptide SUP35, a human-derived elastin and a human-derived adhesive peptide, the amino acid sequence of the self-assembling peptide SUP35 is as shown in SEQ ID NO. 3; the amino acid sequence of the human elastin is as shown in SEQ ID NO. 4; the sequence of the human-derived adhesion peptide is VAPG; the number of the human-derived elastin is one or more. The self-assembled super-elastic protein is obtained by fermenting and purifying by taking microorganisms as a chassis, and a series of problems caused by source problems are reduced. Meanwhile, the super-elastic protein can be self-assembled to form a multi-stage structure, has remarkable functions of high temperature resistance, cell proliferation, cell migration and cell repair, and has remarkable antioxidant capacity and excellent in-vitro adhesion effect.

Compounds comprising a benzaldehyde acetal acid-degradable amphiphilic lipid and self-assembling peptides are incorporated in lipid nanoparticle (LNP) and used to transfect cells.

A nanoagonist, and a preparation method and use thereof are provided, belonging to the technical field of nanoscale biomedicine. The nanoagonist is formed by self-assembly of a transformable peptide, where the transformable peptide includes a targeted antimicrobial peptide, a functionalized self-assembling peptide, an FcγR recognition peptide, and a lipase-responsive hydrophobic molecule that are coupled in sequence. The functionalized self-assembling peptide can control the FcγR recognition peptide to flip toward a surface of a target pathogen during secondary self-assembly, and the target pathogen is a pathogen targeted and bound by the targeted antimicrobial peptide. The nanoagonist combines externalization of the FcγR recognition peptide that can be guided during the secondary self-assembly with FcγR-mediated endocytosis, and a nanoagonist is developed for the first time that takes into account both pathogen clearance and host immune function repair.

The present invention relates to a liquid, positively charged, responsive self- assembling peptide, a liquid scaffold material formed from the self-assembling peptide, and applications of the liquid scaffold material.

According to the echinococcus granulosus vaccine based on the self-assembly peptide, a peptide fragment, rich in dominant epitopes, in EgG1Y162-2 is connected to a Q11 amino terminal through '-SGSG-', due to the self-assembly effect of the Q11 peptide, the peptide fragment rich in dominant epitopes is repeatedly expressed on the surface of nanofibers, and therefore EgG1Y162 dominant multi-epitope self-assembly nano-polypeptide is formed; the EgG1Y162 dominant multi-epitope self-assembled nano polypeptide is adopted in the vaccine, so that the immune recognition and response of a body are effectively enhanced.

The present invention provides a protein-responsive hydrogel and a method for preparing the same. The hydrogel comprises a nanofiber three-dimensional network structure formed by protein-induced self-assembly of a polypeptide or its derivative solution, and can be used for three-dimensional culture in vitro. It is useful for tissue repair, wound dressings, hemostatic materials, dispersion and in-tissue molding materials containing regenerative microspheres such as L-polylactic acid and polycaprolactone for cosmetic and medical use, carriers for the sustained release of drugs or functional factors, and cell preservation materials.

An object is to provide a peptide gelling agent which gels under physiological conditions and which has a relatively short chain length, and a sustained-release gel based on the gelling agent. A hydrogelling self-assembling peptide is provided having one or two core peptides with an amino acid sequence of the formula: Xaa-Yaa-Zaa-Yaa-Xaa-Yaa-Zaa-Yaa-Xaa, wherein Xaa is independently Ile or Met, Yaa is independently Asp, Glu, Lys, or Arg, and Zaa is independently Ala or Gly. The full length of the amino acid sequence constituting the self-assembling peptide is 25 amino acids or less.

The application discloses a kind of peptide micropipe / In2O3 / Au composite gas-sensitive materials, its preparation method and application, belong to gas-sensitive material technical field.The material is self-assembled peptide micropipe (SPMFs) as three-dimensional support skeleton, by solution mixing and functional modification, make In2O3 nanoparticle uniform dispersion, Au nanoparticle surface load, form ternary composite system.SPMFs porous structure provides stable load platform for nanoparticle and accelerates gas diffusion, the LSPR effect of Au nanoparticle can enhance visible light absorption and surface reactivity, the heterojunction formed by In2O3 and SPMFs regulates electron transport.Under the synergistic effect of the three, material can detect ethanol at room temperature under visible light excitation, with high selectivity, high sensitivity and fast adsorption and desorption characteristics, detection lower limit is as low as 1 ppm, meet the demand of trace ethanol real-time monitoring.

There is provided a bioabsorbable peptide tissue occluding agent that can be applied to large mammals including humans, the peptide tissue occluding agent being obtained by artificial synthesis to avoid concerns of infection by viruses and the like.The tissue occluding agent contains a peptide, wherein the peptide is an amphiphilic peptide having 8-200 amino acid residues with the hydrophilic amino acids and hydrophobic amino acids alternately bonded, and is a self-assembling peptide exhibiting a β-structure in aqueous solution in the presence of physiological pH and / or a cation.

To provide a material or a method capable of replacing a conventional digestive fluid leakage preventing material and a conventional digestive fluid leakage preventing method.SOLUTION: The digestive fluid leakage prevention material of the present invention contains a self-assembling peptide gel, has an adhesion residual ratio of at least 40% with respect to a collagen sheet, and has a degradation rate of 35% or less when treated with pancreatic fluid at 37 °C for 7 days.SELECTED DRAWING: None

The present invention provides methods for treating mucosal inflammation or vascular leakage in a subject. The methods involve administering to the subject a pharmaceutical composition comprising RADA16 self-assembling peptide.

The invention is in the field of caries prevention and treatment, in particular minimally-invasive, or non-invasive dental decay treatment. It relates to a composition and a kit comprising a self-assembling peptide, such as the self-assembling peptide P11-4 or P11-8, and a dental agent, namely a dental sealant, e.g. a glass-ionomer cement-based sealant or a resin-based sealant, a fluoride varnish, a dental restorative material or a bonding agent. The invention further relates to a composition and kit comprising a self-assembling peptide and a dental agent for use in the treatment of a carious lesion, preferably a cavitated carious lesion. The self-assembling peptide prevents secondary caries after failure of the dental agent used in treatment of cavitated caries, especially in case of a failing interface between the restoration with the dental agent and the tooth. The composition and kit of the present invention may also be used for pulp capping.

Disclosed herein are methods for induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) by combining the hiPSC-CMs with a self-assembling peptide (SAP). Also disclosed herein are compositions comprising induced pluripotent stem cell-derived cardiomyocytes and a self-assembling peptide.

This invention discloses a β-hairpin structure for self-assembling and capturing an antimicrobial peptide J-R-RF and its application in the preparation of antimicrobial agents, relating to the field of biomedical technology. The amino acid sequence of this β-hairpin structure for self-assembling and capturing an antimicrobial peptide J-R-RF is shown in SEQ ID NO. 6. This invention selects a broad-spectrum antimicrobial short peptide J-1 derived from bee royal jelly as the template peptide. Based on the amino acid sequence characteristics of short peptide J-1, it designs and modifies the structure to obtain alternating hydrophilic and hydrophobic amino acid structural units, which are then used as the backbone of the self-assembled peptide fiber. D The β-hairpin peptide is designed using two turning sequences, PG and RRRF, to achieve a dual antibacterial and bacterial trapping effect. The β-hairpin structure self-assembled to trap antimicrobial peptides provided by this invention can form a fibrous network structure, exhibiting good antibacterial effect and high stability, as well as excellent biocompatibility, providing effective technical support for the development of novel antimicrobial drugs.

The application belongs to the technical field of biological materials, and particularly relates to an enzyme response in-situ self-assembled peptide and anti-tumor application thereof. An amino acid sequence of the self-assembled peptide PSK is shown in SEQ ID NO. 1. The self-assembled peptide PSK of the application firstly combines a PD-L1 blocking peptide with a tumor cell surface PD-L1, effectively internalizes the PSK peptide into the tumor cell through intracellular endocytosis, hydrolyzes in a lysosome through Legumain response bond, triggers self-assembled module assembly to form a beta-folded nanofilament structure, further triggers lysosome membrane permeability change, escapes from the lysosome, releases KLA to destroy mitochondria and induce tumor cell apoptosis. At the same time, the PD-L1 binding peptide is endocytosed into the lysosome, releases immunosuppression, combines with ICD induced by mitochondrial damage, enhances the killing effect of immune cells on tumor cells in the tumor microenvironment, effectively improves the selectivity and anti-tumor efficacy of the polypeptide, and has good biological safety.

The present disclosure provides, in part, peptide self-assemblies that are made into tablet form and methods of making and using the same. In some embodiments, the disclosure provides methods and formulations for a tabletized form of a vaccine, particularly a vaccine comprising self-assembling peptide-polymer nanofibers, an excipient and an adjuvant. Methods of making and using the tablet formulation are also provided.

This invention relates to an improved blister pack (1) for an active agent, such as a peptide that needs to be dissolved before application, for example, a self-assembling peptide, such as P11-4. In particular, the invention provides a blister pack (1) that is convenient to use and less expensive to produce than previous packs, and its use in peptide applications, such as in the treatment of subsurface caries. In a preferred embodiment, the blister pack (1) comprises a blister sheet having at least one first cavity (2), a cover sheet (3) disposed on and attached to the blister sheet, wherein the at least one first cavity (2) comprises a porous carrier (4) coated with the active agent, and advantageously includes a stabilizing cavity (10) that reduces shaking of the blister pack (1) during the dissolution of the active agent, preferably comprising a V-shaped stabilizing cavity (10).

The present invention provides a synthetic, modular system for DNA modification as may be employed for genome editing comprising a targeting nucleic acid possessing both DNA targeting ability and ability to bind a recognition module of a modular polypeptide component, where the modular polypeptide component also includes an effector component and a short peptide DNA-binding sequence (DBD) which binds a pre-determined sequence (PBS) in the target. The DBD may preferably be no more than a 15 mer and serves to destabilize the structure of a targeted dsDNA upon binding thereby facilitating the desired DNA modification. The effector component may, for example, be an artificial nickase comprising linked self-assembling peptides thereby providing a complete genome-editing system of advantageous small size for vector delivery to cells.

The present invention provides new dental care products comprising self-assembling peptides that are capable of undergoing self-assembly at a certain pH for use in dental care, e.g. preventing and / or treating a tooth lesion such as a caries lesion, remineralising a tooth surface or increasing smoothness or shine, or for increasing hardness of a tooth surface. The present invention provides dental care products such as chewing gum, toffees or toothpaste, in which the peptides are present in monomeric form for a prolonged period after application into the oral cavity, thereby enabling non-targeted treatment of a plurality of teeth, independent of the diagnosis of an active lesion. Products of the invention are useful for animals and humans.

Provided herein are self-assembling peptide amphiphiles (PAs) comprising a bioactive Transforming growth factor beta 1 (TGF-β1) mimetic epitope, high-aspect-ratio nanostructures of PAs displaying a TGF-β1 mimetic epitope, and methods of enhancing cartilage regeneration / repair and / or treatment of osteoarthritis and other musculoskeletal injuries and diseases.

The scheme discloses the field of biomedical materials and bone tissue engineering technology, and discloses a composite drug-loaded hydrogel and a preparation method and application thereof.The composite drug-loaded hydrogel comprises a self-assembled peptide hydrogel base, hollow mesoporous dopamine nanoparticles and simvastatin, the simvastatin is loaded in the hollow mesoporous structure of the hollow mesoporous dopamine nanoparticles to form SIM@H-MPDA drug-loaded nanoparticles, and the drug-loaded nanoparticles are stably dispersed in the three-dimensional network structure of RADA16-I.The preparation method of the composite drug-loaded hydrogel is also disclosed, and the preparation method comprises H-MPDA preparation, SIM@H-MPDA drug-loaded nanoparticle preparation and composite hydrogel assembly.The composite drug-loaded hydrogel has good injectability and in-situ gelation, can realize smooth and slow release of simvastatin, significantly promotes osteogenic differentiation and new bone formation, effectively repairs non-infectious bone defects, and has no adverse effects on main organs of the body.