23 results about "Self-assembling peptide" patented technology

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

[Problem] The present invention provides a hemostatic composition and a hemostatic method using the hemostatic composition, and specifically provides a composition for hemostasis that can be uniformly applied to a bleeding site and exert a high hemostatic effect. [Solution] A composition to be applied as a spray to a subject is provided, wherein the composition is characterized in that the spray is for hemostasis, and the composition comprises a self-assembling peptide that gels due to self-assembly when the composition is applied to a bleeding site of the subject, and the self-assembling peptide is contained in the composition at a concentration with an improved hemostatic ability compared to direct application.

The present disclosure describes a peptide scaffold for producing vaccines. The peptide scaffold includes a peptide that self-assembles into a hapten carrier (hC) includes amphipathic alpha-helices. The peptide includes heptad repeats following a specific pattern. The hC further includes hapten or an agent conjugated to it, and optionally the hC includes one or more T-cell epitopes at the N- and / or C-terminus of the one or more amphipathic alpha-helices. The present disclosure also describes compositions including immunogenic compositions including the hapten-hC or agent-hC conjugate.

Compositions containing self-assembling peptides and / or self-assembling peptidomimetics (“self-assembling peptides”) can be used to create a long-lasting “lift” or means of elevating tissue to be resected, dissected, manipulated or repaired, as a bulking agent, or as a tissue forming matrix by injection of a solution that forms a solid gel in situ, which is stable for a prolonged period of time from days to a month, is hemostatic, and may prevent adhesions. These self-assembling peptides and methods of use thereof enable better separation of tissues and visualization of margins, more durable and robust lifts, less need for frequent injections that carry risk of undesired perforation, and simultaneous management of adverse effects, such as bleeding, leaking, inflammation and iatrogenic injury during endoscopic, laparoscopic or other minimally invasive, or open surgical procedures in and / or on the body.

The invention discloses self-assembled super-elastic protein, hydrogel and application of the self-assembled super-elastic protein and the hydrogel, and belongs to the technical field of biology. The self-assembled super-elastic protein provided by the invention comprises a self-assembled peptide SUP35, a human-derived elastin and a human-derived adhesive peptide, the amino acid sequence of the self-assembling peptide SUP35 is as shown in SEQ ID NO. 3; the amino acid sequence of the human elastin is as shown in SEQ ID NO. 4; the sequence of the human-derived adhesion peptide is VAPG; the number of the human-derived elastin is one or more. The self-assembled super-elastic protein is obtained by fermenting and purifying by taking microorganisms as a chassis, and a series of problems caused by source problems are reduced. Meanwhile, the super-elastic protein can be self-assembled to form a multi-stage structure, has remarkable functions of high temperature resistance, cell proliferation, cell migration and cell repair, and has remarkable antioxidant capacity and excellent in-vitro adhesion effect.

Compounds comprising a benzaldehyde acetal acid-degradable amphiphilic lipid and self-assembling peptides are incorporated in lipid nanoparticle (LNP) and used to transfect cells.

A nanoagonist, and a preparation method and use thereof are provided, belonging to the technical field of nanoscale biomedicine. The nanoagonist is formed by self-assembly of a transformable peptide, where the transformable peptide includes a targeted antimicrobial peptide, a functionalized self-assembling peptide, an FcγR recognition peptide, and a lipase-responsive hydrophobic molecule that are coupled in sequence. The functionalized self-assembling peptide can control the FcγR recognition peptide to flip toward a surface of a target pathogen during secondary self-assembly, and the target pathogen is a pathogen targeted and bound by the targeted antimicrobial peptide. The nanoagonist combines externalization of the FcγR recognition peptide that can be guided during the secondary self-assembly with FcγR-mediated endocytosis, and a nanoagonist is developed for the first time that takes into account both pathogen clearance and host immune function repair.

The present invention relates to a liquid, positively charged, responsive self- assembling peptide, a liquid scaffold material formed from the self-assembling peptide, and applications of the liquid scaffold material.

According to the echinococcus granulosus vaccine based on the self-assembly peptide, a peptide fragment, rich in dominant epitopes, in EgG1Y162-2 is connected to a Q11 amino terminal through '-SGSG-', due to the self-assembly effect of the Q11 peptide, the peptide fragment rich in dominant epitopes is repeatedly expressed on the surface of nanofibers, and therefore EgG1Y162 dominant multi-epitope self-assembly nano-polypeptide is formed; the EgG1Y162 dominant multi-epitope self-assembled nano polypeptide is adopted in the vaccine, so that the immune recognition and response of a body are effectively enhanced.

An object is to provide a peptide gelling agent which gels under physiological conditions and which has a relatively short chain length, and a sustained-release gel based on the gelling agent. A hydrogelling self-assembling peptide is provided having one or two core peptides with an amino acid sequence of the formula: Xaa-Yaa-Zaa-Yaa-Xaa-Yaa-Zaa-Yaa-Xaa, wherein Xaa is independently Ile or Met, Yaa is independently Asp, Glu, Lys, or Arg, and Zaa is independently Ala or Gly. The full length of the amino acid sequence constituting the self-assembling peptide is 25 amino acids or less.

The application discloses a kind of peptide micropipe / In2O3 / Au composite gas-sensitive materials, its preparation method and application, belong to gas-sensitive material technical field.The material is self-assembled peptide micropipe (SPMFs) as three-dimensional support skeleton, by solution mixing and functional modification, make In2O3 nanoparticle uniform dispersion, Au nanoparticle surface load, form ternary composite system.SPMFs porous structure provides stable load platform for nanoparticle and accelerates gas diffusion, the LSPR effect of Au nanoparticle can enhance visible light absorption and surface reactivity, the heterojunction formed by In2O3 and SPMFs regulates electron transport.Under the synergistic effect of the three, material can detect ethanol at room temperature under visible light excitation, with high selectivity, high sensitivity and fast adsorption and desorption characteristics, detection lower limit is as low as 1 ppm, meet the demand of trace ethanol real-time monitoring.

There is provided a bioabsorbable peptide tissue occluding agent that can be applied to large mammals including humans, the peptide tissue occluding agent being obtained by artificial synthesis to avoid concerns of infection by viruses and the like.The tissue occluding agent contains a peptide, wherein the peptide is an amphiphilic peptide having 8-200 amino acid residues with the hydrophilic amino acids and hydrophobic amino acids alternately bonded, and is a self-assembling peptide exhibiting a β-structure in aqueous solution in the presence of physiological pH and / or a cation.

The invention is in the field of caries prevention and treatment, in particular minimally-invasive, or non-invasive dental decay treatment. It relates to a composition and a kit comprising a self-assembling peptide, such as the self-assembling peptide P11-4 or P11-8, and a dental agent, namely a dental sealant, e.g. a glass-ionomer cement-based sealant or a resin-based sealant, a fluoride varnish, a dental restorative material or a bonding agent. The invention further relates to a composition and kit comprising a self-assembling peptide and a dental agent for use in the treatment of a carious lesion, preferably a cavitated carious lesion. The self-assembling peptide prevents secondary caries after failure of the dental agent used in treatment of cavitated caries, especially in case of a failing interface between the restoration with the dental agent and the tooth. The composition and kit of the present invention may also be used for pulp capping.

The application belongs to the technical field of biological materials, and particularly relates to an enzyme response in-situ self-assembled peptide and anti-tumor application thereof. An amino acid sequence of the self-assembled peptide PSK is shown in SEQ ID NO. 1. The self-assembled peptide PSK of the application firstly combines a PD-L1 blocking peptide with a tumor cell surface PD-L1, effectively internalizes the PSK peptide into the tumor cell through intracellular endocytosis, hydrolyzes in a lysosome through Legumain response bond, triggers self-assembled module assembly to form a beta-folded nanofilament structure, further triggers lysosome membrane permeability change, escapes from the lysosome, releases KLA to destroy mitochondria and induce tumor cell apoptosis. At the same time, the PD-L1 binding peptide is endocytosed into the lysosome, releases immunosuppression, combines with ICD induced by mitochondrial damage, enhances the killing effect of immune cells on tumor cells in the tumor microenvironment, effectively improves the selectivity and anti-tumor efficacy of the polypeptide, and has good biological safety.

This invention relates to an improved blister pack (1) for an active agent, such as a peptide that needs to be dissolved before application, for example, a self-assembling peptide, such as P11-4. In particular, the invention provides a blister pack (1) that is convenient to use and less expensive to produce than previous packs, and its use in peptide applications, such as in the treatment of subsurface caries. In a preferred embodiment, the blister pack (1) comprises a blister sheet having at least one first cavity (2), a cover sheet (3) disposed on and attached to the blister sheet, wherein the at least one first cavity (2) comprises a porous carrier (4) coated with the active agent, and advantageously includes a stabilizing cavity (10) that reduces shaking of the blister pack (1) during the dissolution of the active agent, preferably comprising a V-shaped stabilizing cavity (10).

Provided herein are self-assembling peptide amphiphiles (PAs) comprising a bioactive Transforming growth factor beta 1 (TGF-β1) mimetic epitope, high-aspect-ratio nanostructures of PAs displaying a TGF-β1 mimetic epitope, and methods of enhancing cartilage regeneration / repair and / or treatment of osteoarthritis and other musculoskeletal injuries and diseases.

The scheme discloses the field of biomedical materials and bone tissue engineering technology, and discloses a composite drug-loaded hydrogel and a preparation method and application thereof.The composite drug-loaded hydrogel comprises a self-assembled peptide hydrogel base, hollow mesoporous dopamine nanoparticles and simvastatin, the simvastatin is loaded in the hollow mesoporous structure of the hollow mesoporous dopamine nanoparticles to form SIM@H-MPDA drug-loaded nanoparticles, and the drug-loaded nanoparticles are stably dispersed in the three-dimensional network structure of RADA16-I.The preparation method of the composite drug-loaded hydrogel is also disclosed, and the preparation method comprises H-MPDA preparation, SIM@H-MPDA drug-loaded nanoparticle preparation and composite hydrogel assembly.The composite drug-loaded hydrogel has good injectability and in-situ gelation, can realize smooth and slow release of simvastatin, significantly promotes osteogenic differentiation and new bone formation, effectively repairs non-infectious bone defects, and has no adverse effects on main organs of the body.

The present disclosure provides peptide compositions (e.g., of self-assembling peptides) with particular attributes (e.g., peptide identity, peptide concentration, pH, ionic strength (including salt identity and / or concentration), etc. that show particularly useful material properties. The present disclosure also provides technologies for selecting and / or formulating particular peptide compositions useful in specific contexts. In some embodiments, provided peptide compositions have an elevated pH within the range of about 2.5 to about 3.5 and / or an ionic strength that is above that of a corresponding composition of the same peptide, at the same concentration, in water, but is below a critical salt point for the peptide (e.g., so that the composition is not cloudy).

The application discloses an enzyme-triggered self-assembly peptide hydrogel, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The hydrogel comprises a peptide precursor Nap-YY(p)Y, an antibacterial compound daphnodorin and alkaline phosphatase; the peptide precursor is dephosphorylated under the action of the alkaline phosphatase, self-assembled to form a nanofiber network structure, and encapsulates the daphnodorin in the network structure. The hydrogel has a short gelation time (3-10 minutes), a moderate mechanical strength (a storage modulus G' of 130-170 Pa), a high encapsulation rate (greater than or equal to 75%), presents a biphasic release characteristic in a simulated wound liquid, and can maintain an effective therapeutic concentration for more than 7 days. The hydrogel has a significant synergistic killing effect on methicillin-resistant Staphylococcus aureus and a biofilm thereof, can effectively reduce a bacterial load of a wound and accelerate wound healing.

The application discloses a kind of self-assembly super elastic protein, hydrogel and its application, belong to the field of biotechnology.The self-assembly super elastic protein of the application includes: self-assembly peptide SUP35, human-derived elastin and human-derived adhesive peptide;The amino acid sequence of the self-assembly peptide SUP35 is as shown in SEQ ID NO.3;The amino acid sequence of the human-derived elastin is as shown in SEQ ID NO.4;The sequence of the human-derived adhesive peptide is VAPG;The number of the human-derived elastin is 1 or more.The self-assembly super elastic protein of the application is obtained by fermentation, purification using microorganism as the bottom disc, reduces a series of problems caused by source problem.Simultaneously super elastic protein can be self-assembled to form multistage structure, has the function of significant high-temperature resistance, cell proliferation, cell migration, cell repair, and has significant antioxidant capacity and excellent in-vitro adhesion.

The present application relates to a kind of detection method of self-assembled peptide hydrogel hexadecapeptide content, belong to biological material detection technical field.The method includes the following steps: first instrument preparation, using high performance liquid chromatography (HPLC) as detection equipment;Secondly, preparation of test solution, about 0.2 g self-assembled peptide hydrogel test sample is placed in centrifugal tube, after being dissolved with 2 mL primary water, drying is carried out in vacuum freeze-drying box;The dry powder obtained is added to 0.2 mL formic acid, extracts 30 min under the condition of 37.0 ℃, then add primary water to constant volume to 10 mL, and vortex mixing, obtain test solution;Finally, the test solution is detected by HPLC and the content of hexadecapeptide is calculated.The method is simple in operation, good in repeatability, accurate in detection result, can effectively realize the quantitative detection of self-assembled peptide hydrogel hexadecapeptide content, and has good application value.

The present invention relates peptides capable of forming a hydrogel by self-assembly, wherein the peptide consists of 6 to 12 amino acids and containing the amino acid sequence X1–A4–A5–A6–X2. Said peptides self-assemble especially under mild conditions in an aqueous dispersion phase thereby forming a hydrogel and are thus particularly useful for forming a hydrogel composition.

The invention relates to the technical field of hydrogel, in particular to functional hydrogel of antibacterial healing-promoting fusion polypeptide as well as a preparation method and application of the functional hydrogel. The self-assembled peptide RADA16 is adopted as a hydrogel base material, and the neuropeptide and the antibacterial peptide are combined, so that the double effects of healing promotion and bacterium resistance of the hydrogel material are realized. The polypeptide and the degradation product amino acid are non-toxic and low in immunogenicity, and the polypeptide hydrogel has huge potential as a wound dressing by virtue of the natural source, biocompatibility and biosafety of the polypeptide hydrogel.