87 results about "Self-assembling peptide" patented technology

The invention provides a multifunctional self-assembly peptide recognition method and system, and relates to the technical field of biological peptide recognition. The method comprises the following steps: firstly, obtaining peptide sequences of a self-assembled peptide and a plurality of biological functional peptides, and inputting the peptide sequences into an embedded layer of a polypeptide function prediction model for 21-dimensional vectorization; and learning combination modes and sequence features of amino acids through an amino acid association feature extraction layer of the LSTM network. Then, acquiring direct contact information among different amino acids on the basis of a fine-grained feature learning layer of a multi-head self-attention mechanism; and finally, designing a Transform multi-task classifier which comprises five sub-task classifiers which are used for respectively predicting the self-assembly function, the anti-cancer function, the anti-inflammatory function, the antibacterial function and the antioxidant function. According to the method, a multi-layer structure is adopted to carry out layer-by-layer deep learning and optimization on polypeptide sequence features, efficient recognition and classification of multifunctional biological peptides are achieved, and finally the model performance is comprehensively evaluated through the accuracy rate, the precision rate, the recall rate and the F1 value.

The invention discloses a beta-hairpin structure self-assembly capture antibacterial peptide and application thereof, and relates to the technical field of biological medicine. The amino acid sequence of the beta-hairpin structure self-assembly capture antibacterial peptide is as shown in any one of SEQ ID NO. 3-6. A broad-spectrum antibacterial short peptide Jelleine-1 (J-1) from bee royal jelly is selected as a template peptide, design and transformation are performed according to amino acid sequence characteristics of the short peptide J-1, and a hydrophilic and hydrophobic amino acid alternate arrangement structural unit is obtained and serves as a self-assembly peptide fiber skeleton. Beta-hairpin peptide design is carried out by adopting two turning sequences of DPG and RRRF, so that the beta-hairpin peptide has dual effects of resisting bacteria and capturing bacteria. The beta-hairpin structure self-assembly capture antibacterial peptide provided by the invention can form a fiber network structure, has a good antibacterial effect, high stability and excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

The invention relates to the technical field of molecular biology and protein engineering, in particular to a lactic acid bacteria antibacterial peptide LABA4 multi-copy expression method and application. The invention aims to provide a prokaryotic expression method of an antibacterial peptide LAB4 tandem gene based on a self-assembled peptide and application, so that the problems of toxicity to a host, low expression quantity and non-ideal product activity in a large intestine expression system are solved, the antibacterial peptide is efficiently expressed in escherichia coli, and the antibacterial activity is effectively improved. Particularly, the characteristics of specific cutting and no amino acid residue of enterokinase are utilized, modified recombinant enterokinase is added between LAB4 monomers to serve as an interval restriction enzyme cutting site, a gene shuffling technology is utilized to design and construct three-six copy tandem recombinant plasmids of LAB4, the self-cleavage characteristic of intein is utilized, and the recombinant enterokinase recombinant plasmids of LAB4 can be used for preparing the recombinant enterokinase recombinant plasmids of LAB4, so that the recombinant enterokinase recombinant plasmids of LAB4 can be used for preparing the recombinant enterokinase recombinant plasmids of LAB4. An optimized cspA cold shock promoter in a pCold I vector is adopted, and high-efficiency expression of tandem LAB4 in an escherichia coli expression system is realized through 15 DEG C low-temperature induction precise regulation and control.

The invention relates to an SR-B1 targeted polypeptide compound and application thereof in preparation of antitumor drugs, and belongs to the technical field of biological drug manufacturing. In order to solve the problems that the existing PDT photosensitizer is insufficient in targeting property and the treatment effect is limited due to the action of intracellular cholesterol, the invention provides an SR-B1 targeting polypeptide compound which is composed of an SR-B1 targeting polypeptide and an AS1411 aptamer. The SR-B1 targeting polypeptide comprises an SR-B1 targeting peptide fragment, a self-assembly peptide fragment, a response enzyme digestion peptide fragment and a photosensitizer TCPP which are connected in sequence. The invention constructs a polypeptide targeting system based on a dual self-assembly strategy, which not only can target SR-B1, inhibit a cholesterol-mediated tumor for a long time to promote a signal channel and interfere the ROS steady state, but also can target TCPP to a cell nucleus, improve the photosensitivity of PDT, and further improve the killing effect on tumor cells by improving the yield of ROS to cause the damage to the cell nucleus.

The invention relates to an SR-B1 targeted polypeptide compound and application thereof in preparation of antitumor drugs and immunotherapy sensitizers, and belongs to the technical field of biological drug manufacturing. In order to solve the problems that an existing PD-L1 inhibitor is single in action mechanism and immune system dysfunction is easily caused, the invention provides an SR-B1 targeted polypeptide compound which is composed of SR-B1 targeted polypeptide and Resiquimod. The SR-B1 targeting polypeptide comprises an SR-B1 targeting peptide fragment, a self-assembly peptide fragment and a hydrophobic molecule which are connected in sequence. The SR-B1 targeting polypeptide can recognize overexpressed SR-B1 in tumor cells, block cholesterol uptake, inhibit tumor progression and inhibit expression of PD-L1 at the same time. The polypeptide compound accurately delivers Resiquimod to a tumor site, anti-tumor immune response is activated, and sensitivity of tumor immunotherapy is greatly enhanced through combination of cholesterol metabolism regulation and immunotherapy.

The invention relates to the technical field of polypeptide drugs, in particular to a nanoscale mitochondrial DNA scavenger and application thereof. The polypeptide targeting myocardial cells in the nanoscale mitochondrial DNA scavenger provided by the invention can target the myocardial cells, so that the STING inhibitor is enriched in the myocardial cells; the mtDNA probe can be combined with mtDNA in a targeted manner to form an NS-mtDNA compound; the self-assembly peptide is a convertible self-assembly unit, forms a nano structure, can transport an NS-mtDNA compound to lysosome to promote degradation, can prolong the retention time and stability of the nano-scale mitochondrial DNA scavenger and continuously release an STING inhibitor, the released STING inhibitor can inhibit an STING pathway, and the self-assembly peptide can be used for inhibiting the activity of the nano-scale mitochondrial DNA scavenger, so that the activity of the nano-scale mitochondrial DNA scavenger can be improved, and the activity of the nano-scale mitochondrial DNA scavenger is improved. Therefore, myocarditis or inflammations related to myocarditis are improved, and a new technical support is provided for treatment of myocarditis.

Embodiments are directed to a UPEC conjugate peptide including a self-assembling peptide and at least one UPEC epitope. The UPEC conjugate peptide may self-assemble into a nanofiber or fibril. Compositions including the UPEC conjugate peptide may be used to treat a bacterial infection such as a urinary tract infection (UTI). The compositions and methods may be specific for pathogenic bacteria. The compositions and methods may treat the infection without altering the gut microbiome.

The application discloses a kind of β-hairpin structure self-assembly capture antibacterial peptide J-1-RF and its application, it is related to biological medicine technical field.The amino acid sequence of the β-hairpin structure self-assembly capture antibacterial peptide J-1-RF is as shown in SEQ ID NO.4.The application selects the broad-spectrum antibacterial short peptide J-1 derived from honeybee royal jelly as template peptide, designs and transforms according to the amino acid sequence characteristics of short peptide J-1, obtains hydrophilic and hydrophobic amino acid alternately arranged structure unit, and uses it as self-assembly peptide fiber skeleton. D PG and RRRF two turning sequences are used for β-hairpin peptide design, so that it has the dual effect of antibacterial and bacterial capture.The β-hairpin structure self-assembly capture antibacterial peptide J-1-RF provided by the application can form fiber network structure, has good bacteriostatic effect and high stability, and has excellent biocompatibility, which provides effective technical support for developing new antibacterial drugs.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The present invention provides a material or method that can replace conventional materials and methods for preventing digestive fluid leakage. The digestive fluid leakage preventing material of the present invention comprises a self-assembling peptide gel, has an adhesion retention rate of at least 40% on a collagen sheet, and a decomposition rate of 35% or less based on pancreatic juice treatment at 37°C for 7 days.

The invention relates to a polypeptide for targeted destruction of lipid droplets and application of the polypeptide in preparation of antitumor drugs, and belongs to the technical field of biological drug manufacturing. In order to solve the problem that an existing anti-tumor drug aiming at tumor lipid droplets cannot effectively inhibit immune escape of tumor cells while destroying the lipid droplets, the invention provides a polypeptide for targeted destruction of the lipid droplets, and the polypeptide comprises a PLIN2 targeted peptide fragment, a self-assembly peptide fragment, an enzyme response peptide fragment and an HLA-E targeted peptide fragment which are connected in sequence. On the basis of a double-targeting strategy, the polypeptide for targeted destruction of the lipid droplets is constructed, and the polypeptide can target the PLIN2 protein on the surfaces of the lipid droplets; the self-assembled peptide fragment can form a nanofiber structure on the surface of the lipid droplet, the structure and function of the lipid droplet are destroyed, endoplasmic reticulum stress is caused, and tumor cell immunogenicity death is caused. The HLA-E targeting peptide fragment released by polypeptide digestion can specifically target HLA-E on a tumor cell membrane, block an NKG2A-HLA-E pathway, and effectively inhibit immune escape of tumor cells.

[Problem] The present invention provides a hemostatic composition and a hemostatic method using the hemostatic composition, and specifically provides a composition for hemostasis that can be uniformly applied to a bleeding site and exert a high hemostatic effect. [Solution] A composition to be applied as a spray to a subject is provided, wherein the composition is characterized in that the spray is for hemostasis, and the composition comprises a self-assembling peptide that gels due to self-assembly when the composition is applied to a bleeding site of the subject, and the self-assembling peptide is contained in the composition at a concentration with an improved hemostatic ability compared to direct application.

The present disclosure describes a peptide scaffold for producing vaccines. The peptide scaffold includes a peptide that self-assembles into a hapten carrier (hC) includes amphipathic alpha-helices. The peptide includes heptad repeats following a specific pattern. The hC further includes hapten or an agent conjugated to it, and optionally the hC includes one or more T-cell epitopes at the N- and / or C-terminus of the one or more amphipathic alpha-helices. The present disclosure also describes compositions including immunogenic compositions including the hapten-hC or agent-hC conjugate.

Compositions containing self-assembling peptides and / or self-assembling peptidomimetics (“self-assembling peptides”) can be used to create a long-lasting “lift” or means of elevating tissue to be resected, dissected, manipulated or repaired, as a bulking agent, or as a tissue forming matrix by injection of a solution that forms a solid gel in situ, which is stable for a prolonged period of time from days to a month, is hemostatic, and may prevent adhesions. These self-assembling peptides and methods of use thereof enable better separation of tissues and visualization of margins, more durable and robust lifts, less need for frequent injections that carry risk of undesired perforation, and simultaneous management of adverse effects, such as bleeding, leaking, inflammation and iatrogenic injury during endoscopic, laparoscopic or other minimally invasive, or open surgical procedures in and / or on the body.

The present invention provides a self-assembling peptide gel for use in preventing a leak of digestive fluid and a self-assembling peptide gel for use in protecting an organ against digestion by a digestive fluid. The self-assembling peptide gel comprises a self-assembling peptide and water, whereby the self-assembling peptide gel has an adhesion persistence ratio with respect to a collagen sheet of at least 40% and a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days, and the self-assembling peptide gel is positively charged as a whole.

Disclosed herein is a versatile injectable nano-bioactive scaffold designed to achieve both immediate soft tissue volumization and long-term tissue regeneration. By utilizing biocompatible polymers such as hyaluronic acid, chitosan, and alginate, along with self-assembling peptides and nano-structured carriers including mesoporous silica nanoparticles and liposomes, the system facilitates controlled release of essential growth factors. This innovative approach delivers sustained bioactivity, significantly reducing the need for repeated injections and minimizing inflammatory complications. The system promises superior clinical outcomes, advancing the field of tissue engineering and regenerative medicine by providing an effective and lasting solution for tissue repair and regeneration.

The invention discloses self-assembled super-elastic protein, hydrogel and application of the self-assembled super-elastic protein and the hydrogel, and belongs to the technical field of biology. The self-assembled super-elastic protein provided by the invention comprises a self-assembled peptide SUP35, a human-derived elastin and a human-derived adhesive peptide, the amino acid sequence of the self-assembling peptide SUP35 is as shown in SEQ ID NO. 3; the amino acid sequence of the human elastin is as shown in SEQ ID NO. 4; the sequence of the human-derived adhesion peptide is VAPG; the number of the human-derived elastin is one or more. The self-assembled super-elastic protein is obtained by fermenting and purifying by taking microorganisms as a chassis, and a series of problems caused by source problems are reduced. Meanwhile, the super-elastic protein can be self-assembled to form a multi-stage structure, has remarkable functions of high temperature resistance, cell proliferation, cell migration and cell repair, and has remarkable antioxidant capacity and excellent in-vitro adhesion effect.

Compounds comprising a benzaldehyde acetal acid-degradable amphiphilic lipid and self-assembling peptides are incorporated in lipid nanoparticle (LNP) and used to transfect cells.

A nanoagonist, and a preparation method and use thereof are provided, belonging to the technical field of nanoscale biomedicine. The nanoagonist is formed by self-assembly of a transformable peptide, where the transformable peptide includes a targeted antimicrobial peptide, a functionalized self-assembling peptide, an FcγR recognition peptide, and a lipase-responsive hydrophobic molecule that are coupled in sequence. The functionalized self-assembling peptide can control the FcγR recognition peptide to flip toward a surface of a target pathogen during secondary self-assembly, and the target pathogen is a pathogen targeted and bound by the targeted antimicrobial peptide. The nanoagonist combines externalization of the FcγR recognition peptide that can be guided during the secondary self-assembly with FcγR-mediated endocytosis, and a nanoagonist is developed for the first time that takes into account both pathogen clearance and host immune function repair.

The invention discloses an antibacterial peptide J-R-pG and application thereof in preparation of an antibacterial agent, and relates to the technical field of biological medicines. The amino acid sequence of the antibacterial peptide J-R-pG is as shown in SEQ ID NO. 5. A broad-spectrum antibacterial short peptide Jelleine-1 (J-1) from bee royal jelly is selected as a template peptide, design and transformation are performed according to amino acid sequence characteristics of the short peptide J-1, and a hydrophilic and hydrophobic amino acid alternate arrangement structural unit is obtained and serves as a self-assembly peptide fiber skeleton. Beta-hairpin peptide design is carried out by adopting two turning sequences of DPG and RRRF, so that the beta-hairpin peptide has dual effects of resisting bacteria and capturing bacteria. The antibacterial peptide J-R-pG provided by the invention can form a fiber network structure, has a good antibacterial effect, high stability and excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

Described herein are self-assembled peptides and hydrogels comprising self-assembled peptides and glucose. Exemplary hydrogels comprising self-assembled peptides and glucose may be used to encapsulate glucagon and / or a glucagon analogue. Hydrogels with glucagon and / or a glucagon analogue encapsulated therein may be used to treat hypoglycemia and related disorders in subjects in need thereof.

The invention relates to the technical field of stem cells, in particular to a preparation method of neural stem cells, which is characterized by comprising the following steps: separating cells from a nervous tissue sample examined and approved by ethics; mixing the separated cells with the magnetic nanoparticles modified by the specific antibody; separating and recovering the target neural stem cells under the action of a low-gradient magnetic field; inoculating the recovered neural stem cells into a self-assembled peptide hydrogel culture system for in-vitro amplification; performing cryopreservation on the amplified neural stem cells, and performing standardized quality detection; the membrane integrity and surface marker expression of the neural stem cells are maintained through combination of low-concentration enzyme digestion and mild mechanical oscillation, the recovery rate and purity of the cells are remarkably improved by adopting the antibody-modified magnetic nanoparticles and a low-gradient magnetic field sorting technology, the in-vivo microenvironment is simulated by using the self-assembled peptide hydrogel, and the in-vivo microenvironment of the neural stem cells is simulated by using the self-assembled peptide hydrogel. The amplification and differentiation potential of the cells is promoted, so that the preparation efficiency and stability of the neural stem cells are improved.

The invention relates to a self-assembling peptide, a hydrogel formed by the self-assembling peptide, and an application of the hydrogel.

The present invention relates to a liquid, positively charged, responsive self- assembling peptide, a liquid scaffold material formed from the self-assembling peptide, and applications of the liquid scaffold material.

The present disclosure describes peptide nanosponges comprising a plurality of self-assembled peptide building blocks. The plurality of self-assembled peptide building blocks comprises a first peptide building block comprising: a branched polymeric core comprising at least three arms; a first block co-peptide covalently linked to one of said arms, wherein said first block co-peptide comprises a first peptide block and a second peptide block which is different from the first peptide block; a lipid-based capping moiety covalently attached to the first block co-peptide; and a therapeutically active compound covalently attached via a cleavable linkage to the first block co-peptide. Advantageously, the block co-peptide is covalently attached to the core such that it is resistant to enzymatic cleavage from the core.

According to the echinococcus granulosus vaccine based on the self-assembly peptide, a peptide fragment, rich in dominant epitopes, in EgG1Y162-2 is connected to a Q11 amino terminal through '-SGSG-', due to the self-assembly effect of the Q11 peptide, the peptide fragment rich in dominant epitopes is repeatedly expressed on the surface of nanofibers, and therefore EgG1Y162 dominant multi-epitope self-assembly nano-polypeptide is formed; the EgG1Y162 dominant multi-epitope self-assembled nano polypeptide is adopted in the vaccine, so that the immune recognition and response of a body are effectively enhanced.

Compositions containing self-assembling peptides and / or self-assembling peptidomimetics (“self-assembling peptides”) can be used to create a long-lasting “lift” or means of elevating tissue to be resected, dissected, manipulated or repaired, as a bulking agent, or as a tissue forming matrix by injection of a solution that forms a solid gel in situ, which is stable for a prolonged period of time from days to a month, is hemostatic, and may prevent adhesions. These self-assembling peptides and methods of use thereof enable better separation of tissues and visualization of margins, more durable and robust lifts, less need for frequent injections that carry risk of undesired perforation, and simultaneous management of adverse effects, such as bleeding, leaking, inflammation and iatrogenic injury during endoscopic, laparoscopic or other minimally invasive, or open surgical procedures in and / or on the body.

The present invention provides a protein-responsive hydrogel and a method for preparing the same. The hydrogel comprises a nanofiber three-dimensional network structure formed by protein-induced self-assembly of a polypeptide or its derivative solution, and can be used for three-dimensional culture in vitro. It is useful for tissue repair, wound dressings, hemostatic materials, dispersion and in-tissue molding materials containing regenerative microspheres such as L-polylactic acid and polycaprolactone for cosmetic and medical use, carriers for the sustained release of drugs or functional factors, and cell preservation materials.

The present disclosure provides, in part, peptide self-assemblies that are made into tablet form and methods of making and using the same. In some embodiments, the disclosure provides methods and formulations for a tabletized form of a vaccine, particularly a vaccine comprising self-assembling peptide-polymer nanofibers, an excipient and an adjuvant. Methods of making and using the tablet formulation are also provided.

The invention discloses a microsphere filler, and belongs to the field of biological medicine, the filler comprises polypeptide and microspheres capable of forming a net-shaped support, self-assembled peptide molecules in the preparation form a complex with a net-shaped structure in an aqueous solution, the dispersion and support effects can be achieved without modifying the microspheres, and the self-assembled peptide molecules in the preparation can be used for preparing the microsphere filler. And the problem of difficult injection due to microsphere aggregation is avoided.