30results about How to "Improve membrane permeability" patented technology

The invention discloses a 1,3,4-oxadizaole structure unit-containing Rhodamine B pH fluorescence probe, the structure of which is shown as the formula (I) in the specification. The invention further discloses applications of the probe to monitoring the change of pH values and specifically marking lysocome in living cells. The probe does not emit fluoresce under the neutral and alkaline conditions; after the pH value is smaller than 7, the fluorescence intensity is fast enhanced along with the reduction of the pH value and can achieve the maximum value when the pH value is about 4.4; the pH sensitivity of the fluorescence detection ranges from 6.7 to 4.4, and the fluorescence intensity and the pH are in linear relation from pH 4.7 to 5.7; good capability of interference resistance to various metal ions can be achieved under acidity condition; and the probe is very suitable for monitoring the pH value under the complicated interior environment of the living cells in real time, especially specifically marking lysocome and monitoring the change of pH value in the lysocome.

The invention belongs to the field of production of high-added-value products by microorganisms, and particularly relates to a fermentation culture method for producing schizophyllan from schizophyllum commune. The method comprises the following steps of: performing deep liquid culture on schizophyllum commune by using a basic culture medium, and then adding cyclopentanone fatty acid to continue culture. The fermentation culture medium and the cyclopentanone fatty acid additive provided by the invention can shorten the fermentation period of schizophyllum commune hyphae, and greatly improve the content of high-added-value products produced by unit mycelium.

The invention belongs to the technical field of medicines and especially relates to a medicinal application of collagen protein peptide, a cucurbitacin oral micelle, and a preparation method thereof.The application is that the collagen protein peptide is used as a medicine transmembrane tool in preparation of the oral medicine preparation, wherein the collagen protein peptide carries positive charges. The invention provides the cucurbitacin oral micelle containing the collagen protein peptide, which comprises, by weight, 8-15 parts of cucurbitacin, 30-70 parts of lecithin, 50-75 parts of octanoic decanoic polyethylene glycol glyceride, 25-50 parts of polyethylene glycol 15-hydroxystearate and 80-100 parts of the collagen protein peptide carrying the positive charges. The collagen proteinpeptide, cooperated with other carrier materials, can improve membrane-permeability of the medicine and reduce excretion of the medicine, so that the collagen protein peptide can be used as the medicine transmembrane tool. For solving problems about oral bioavailability of the cucurbitacin, the invention provides the technology of collagen protein peptide coating nano-micelle, wherein by means ofthe characters of the collagen protein peptide, the problem of low bioavailability of medicines, such as the cucurbitacin, is solved.