398 results about "Photodynamic therapy" patented technology

The invention discloses a drug-loaded liposome nano-particle, a preparation method thereof and application of the drug-loaded liposome nano-particle in treatment of brain glioma, and belongs to the technical field of biological nano-medicines. The invention provides a novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle and application of the novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle to treatment of brain glioma on the cellular level. The nano-particles are obtained by wrapping copper phthalocyanine derivatives and catalase with lipidosome prepared by a microfluidic method. The co-drug-loaded liposome can catalyze excessive H2O2 in brain glioma to generate oxygen, and O2 is continuously supplied to photodynamic therapy (PDT) to generate active oxygen; meanwhile, Cu < 2 + > can deplete glutathione and improve the oxidative stress level in the glioma, the reduction product Cu < + > can convert H2O2 into hydroxyl radicals with higher toxicity through a Fenton-like reaction, oxidative stress injury is doubly amplified, cascade cooperation of PDT and chemical dynamic therapy (CDT) is achieved, and the treatment effect on the glioma is remarkably enhanced.

The invention discloses a method for preparing a methylacryloyl chitosan / gelatin-based hydrogel wound dressing through 3D printing and application, and relates to a method for preparing a hydrogel wound dressing and application. The problems that existing MXene is high in photo-thermal conversion temperature, and bacteria rapidly breed again after near-infrared irradiation is closed are solved. The ZGC composite hydrogel wound dressing integrates multiple functions, can simultaneously realize hemostasis, antibiosis, anti-inflammation, vascularization promotion and cell regeneration, and is especially suitable for infectious wounds. The antibacterial performance can be achieved under the activation of near-infrared light through the dual effects of a photothermal therapy and a photodynamic therapy, and meanwhile, the antibacterial effect can be achieved in cooperation with Zn < 2 + >. The ZGC composite hydrogel wound dressing can be accurately customized and printed through a 3D printing technology according to the shape and depth of a wound, the composite dressing can achieve accurate low-temperature photo-thermal treatment, meanwhile, the continuous release of Zn < 2 + > and the physical antibacterial function of MXene are maintained, normal tissue is protected while bacteria are killed, and the healing rate of an infected wound is remarkably increased.

The invention relates to a structural design, synthesis and application of a biomass carbon dot modified poly L-cysteine-S-S-carboxylic acid amphiphilic polypeptide. The invention provides a structural design, synthesis and application of poly (L-cysteine-S-S-carboxylic acid) amphiphilic polypeptide (P-L-cys) modified by biomass carbon dots (CDs). Comprising synthesis of P-L-cys which contains hydrophobic groups, provides hydrophilic and functionalized carboxyl and provides reduction response by disulfide bonds, synthesis of CDs and CDs modified P-L-cys (CDs (at) P-L-cys), and the macroscopic performance change of the polypeptide is explored from regulation factors of a polypeptide secondary structure and a self-assembly nanostructure assembly mechanism. The P-L-cys and the CDs (at) P-L-cys prepared by the invention have functionalization, fluorescence imaging and dual-response drug release and have particle sizes suitable for drug delivery and enrichment, and the CDs also provide a photo-thermal and photodynamic synergistic treatment effect and have good application prospects in the fields of drug delivery, cancer resistance and the like.

The invention belongs to the technical field of medicines, and discloses a near-infrared cyanine probe for detecting penicillin G acylase as well as a preparation method and application of the near-infrared cyanine probe. According to the research, a near-infrared fluorescent probe Cy-NEO-PA responding to penicillin G acylase (PGA) is developed, so that the influence of environmental factors on the formation of an acinetobacter baumannii biological membrane is observed. Research results show that glucose inhibits the generation of PGA to enhance the formation of a biological membrane, while phenylacetic acid (PAA) stimulates the generation of PGA and inhibits the formation of the biological membrane. The observation results highlight the excellent capability of Cy-NEO-PA in accurately measuring PGA kinetics, and reveal the key effect of PGA in biological membrane development. In addition, the Cy-NEO-PA has good biocompatibility, strong active oxygen generation, efficient photo-thermal conversion and bacterial targeting ability, so that the Cy-NEO-PA becomes a promising drug for resisting bacterial infection and promoting wound healing through photo-thermal / photodynamic therapy.

The invention discloses an anti-tumor intelligent double-crosslinking hydrogel drug delivery system based on a purple phosphorus nanosheet and a preparation method and application thereof, the system comprises a hydrogel carrier, a photodynamic carrier material and a drug, and the hydrogel carrier is a drug delivery carrier formed by crosslinking polydimethyldiguanide with Fe < 3 + > and a crosslinking agent; the photodynamic carrier material is a purple phosphorus nanosheet; the medicine is a medicine with two effects of inhibiting hypoxia-inducible factor-1 alpha and lowering PD-L1 down. According to the invention, the purple phosphorus nanosheet and the polydimethyldiguanide / Fe < 3 + > bi-crosslinking hydrogel are taken as carriers to prepare the tumor microenvironment response type hydrogel drug delivery system with good pH responsiveness, injectability and self-healing property, so that an effective solution is provided for tumor precise drug delivery mediated by a new material, namely the purple phosphorus nanosheet, as a nano-drug carrier; the invention also provides an effective photodynamic therapy strategy for anti-tumor application of the intelligent double-crosslinking hydrogel and precise treatment of breast cancer.

The invention belongs to the technical field of antitumor drugs, and particularly discloses a preparation method and application of a compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment. The compound for near-infrared fluorescence imaging and photothermal-photodynamic synergistic treatment is shown in the following structural formula: # imgabs0, the molecular skeleton of the compound contains a triphenylamine unit and an N, N-dimethylaniline fragment, intramolecular rotation limitation and intramolecular vibration limitation can be generated, and obvious aggregation-induced emission (AIE) performance is shown. The function-integrated phototherapy agent has high active oxygen generation efficiency, photothermal conversion efficiency and near-infrared fluorescence quantum yield, and is beneficial to obtaining ideal photothermal-photodynamic therapy effect for deep tumors. The phototherapy agent is novel in structure, the preparation method is easy in raw material obtaining, the synthesis condition is mild, the preparation method is simple, purification is convenient and fast, implementation is easy, and the phototherapy agent has great application prospects.

The invention relates to an experimental system and method for photodynamic therapy, photothermal therapy and combined research of photodynamic therapy and photothermal therapy. The experiment system comprises an optical irradiation module which comprises a plurality of laser light sources, each laser light source can output collimated light beams, the collimated light beams are combined to form a coaxial light path, and the coaxial light paths are projected to the surface of an observation object through a light beam adjusting assembly; the light path position adjusting module comprises an electric Z-axis displacement mechanism; the temperature monitoring module comprises an active temperature control substrate, an infrared thermal imager and an optical fiber temperature probe interface; the data acquisition and control module is configured to synchronously acquire laser output power, light spot area, electric Z-axis position, temperature of the active temperature control substrate, temperature sequences of a plurality of different areas and optical fiber temperature probe data, and associatively record the data with a unified timestamp; the power density can be calculated based on the real-time output power of the laser light source and the light spot area; and when the data of any temperature monitoring channel exceeds a preset safety threshold value, the laser light source is automatically controlled to close the output.

The invention belongs to the technical field of biology, and particularly relates to a construction method of a TMV and AIE hybrid material as well as a product and application of the TMV and AIE hybrid material. By optimizing TMV purification conditions, the problems of strip dispersion, strip taking difficulty and the like after ultracentrifugation are effectively solved. After TMV is extracted and purified, AIE is dissolved in a KP buffer solution to prepare an AIE molecular solution, then the purified TMV and the AIE molecular solution are mixed and incubated according to the volume ratio of 10: 1, the hybrid material with fluorescence tracing and photodynamic therapy activity is obtained, in-vivo and in-vitro visual tracking imaging and reactive oxygen species (ROS) release functions are achieved, the diagnosis and treatment integrated potential is shown, and the application prospect is wide. And the application boundary of the TMV in the fields of precision medicine and the like is expanded.

The invention discloses an H-aggregation type I photosensitizer as well as a preparation method and application thereof. The structural formula of the H-aggregation type I photosensitizer disclosed by the invention is # imgabs0. The H-aggregation type I photosensitizer disclosed by the invention is characterized in that aminophenylboronic acid and 5-bromothieno [3, 2-b] thiophene-2-formaldehyde are subjected to a reaction to obtain an intermediate product NBSCHO, and the NBSCHO and 3-(dicyanomethylene) indanone IC are subjected to a reaction to obtain the H-aggregation type I photosensitizer. The photosensitizer disclosed by the invention has a D-pi-A type molecular structure, and due to a well-designed plane characteristic between a pi structure and an A structure, the photosensitizer has relatively strong pi-pi accumulation capacity, and formation of H-aggregation accumulation of the photosensitizer is driven, so that the active oxygen generation effect of molecules, especially generation of I type active oxygen, is improved. The material can efficiently generate I-type active oxygen under the irradiation of white light or near-infrared laser, so that tumor cells are efficiently killed, and an efficient photodynamic therapy effect is shown.

The invention relates to a photodynamic therapy system for treating bladder cancer, and belongs to the technical field of photodynamic therapy. The system comprises a treatment catheter assembly used for performing laser energy delivery and biosensing signal acquisition on a bladder lesion area; the central control unit is used for data processing and control signal generation, and the central control unit can obtain tissue oxygen partial pressure and singlet oxygen concentration according to the biosensing signals collected by the treatment catheter assembly and generate control signals used for regulating and controlling laser energy delivery operation of the treatment catheter assembly according to the tissue oxygen partial pressure and the singlet oxygen concentration; the treatment catheter assembly can perform laser energy delivery operation on tumor tissue in a bladder lesion area in an intermittent mode, the interval time length and applied illumination parameters are changed based on the real-time concentration change condition of singlet oxygen, and the illumination parameters comprise light intensity and wavelength.

The invention discloses a fluorescent / photoacoustic dual-mode diagnosis and treatment probe for detection and photodynamic removal of senescent cells, a preparation method and application, the probe takes a sulfur-substituted hemicyanine dye as a parent structure, a novel bromo-glycoside ligand is covalently coupled, and a blue-green solid is obtained after gradient purification. The probe system shows a remarkable optical response characteristic on beta-galactosidase (beta-Gal) and ideal singlet oxygen (1O2) generation efficiency; an optical signal and a photodynamic therapy effect of the probe can be specifically activated by beta-galactosidase (beta-Gal) overexpressed in cancerous cells and senescent cells under a light excitation condition; the result is successfully verified in a palbociclib-induced senescence melanoma model. According to the probe disclosed by the invention, collaborative regulation and control of dual-mode visual monitoring and precise photodynamic therapy of beta-Gal enzyme activity are realized for the first time, a novel tool is provided for researching a molecular mechanism of cell aging and developing a diagnosis and treatment strategy of aging-related diseases, and the probe has a remarkable application value in the fields of anti-tumor treatment and degenerative diseases.

Methods and systems are described for implementing a photodynamic therapy. An example method may comprise determining, during photodynamic therapy and using a plurality of optical probes spatially distributed within a patient, data indicative of one or more of a photodynamic therapy dosage, a fluence rate of a photodynamic therapy treatment light, or a reactive oxygen species concentration associated with corresponding locations of the plurality of optical probes. The example method may comprise changing, based on the data, one or more treatment parameters associated with providing a photodynamic therapy.

The invention belongs to the technical field of biological medicine and nanotechnology, and particularly relates to nanoparticles loaded with curcumin and antibacterial peptide as well as a preparation method and application of the nanoparticles. The nanoparticles loaded with the curcumin and the antibacterial peptide are prepared by the following steps: taking PEI-PLGA and the curcumin as raw materials, and preparing PEI-PLGA-Cur nanoparticles; pEI-PLGA-Cur nanoparticles and the antibacterial peptide are used as raw materials, and the nanoparticles loaded with the curcumin and the antibacterial peptide are prepared. The nanoparticles loaded with the curcumin and the antibacterial peptide provided by the invention have a photodynamic therapy effect, the drug loading capacity of the curcumin in the nanoparticles is relatively high, the loading rate of the antibacterial peptide is relatively large, auxiliary materials are safe, the preparation process is simple, the particle size of the nanoparticles is relatively small and uniform, the blood compatibility is good, the nanoparticles can be used for treating infection of drug-resistant bacteria, and the application prospect is wide. The potential of becoming a novel nano antibacterial drug and the clinical application prospect are realized.

The invention discloses a triarylmethane type I photosensitive dye as well as a synthesis method and application thereof. The structure of the dye is shown as a general formula I; intramolecular charge separation is optimized by regulating substituent groups, and the generation efficiency of superoxide anions and hydroxyl radicals is remarkably improved. According to the synthesis method, 4-diphenylaminobenzaldehyde or 4-vinyl benzaldehyde is used as a raw material, efficient preparation is achieved through the steps of condensation, oxidation and the like, the raw material is easy to obtain, and operation is easy and convenient. The dye has strong absorption at the wavelength of 600-630 nm, is suitable for red light triggered photodynamic therapy (PDT), especially keeps high reactive oxygen yield under the hypoxic condition, and breaks through the oxygen dependence limitation of traditional PDT. Experiments show that the dye can induce ferroptosis of tumor cells, has the functions of fluorescence imaging and treatment, and has wide application prospects in the fields of antitumor drugs, in-vivo fluorescence labeling, photoresponsive functional preparations and the like.

The application discloses an iron / copper-porphyrin nanoparticle and a preparation method and application thereof, and the nanoparticle is prepared through the following steps: adding iron chloride, copper chloride, tetra(4-carboxyphenyl)porphyrin and benzoic acid into a reaction solvent N,N-dimethylformamide solution, uniformly stirring at room temperature, and reacting at high temperature; and after the reaction is completed, the iron / manganese-porphyrin nanoparticle is obtained through centrifugation and washing. The iron / manganese-porphyrin nanoparticle is prepared by using a brand-new method, the nanoparticle can be degraded in a glutathione solution and release iron ions and copper ions, is used for catalyzing hydrogen peroxide to be converted into a hydroxyl radical (•OH), and tetra(4-carboxyphenyl)porphyrin can generate singlet oxygen (O2) under 660 nm laser irradiation, and is expected to become a good nano-carrier for accelerating photodynamic therapy-chemical kinetic therapy. 1 O2).

The invention specifically discloses a beta-diketone iridium complex as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The beta-diketone iridium complex provided by the invention has high phototoxicity and low dark toxicity, can be applied to tumor cells as effective PSs of PDT, and is specifically delivered to the tumor cells by taking a traditional Chinese medicine monomer tripterine as a supplement in combination with a nano-targeting delivery technology for photodynamic / immune synergistic treatment of hypoxic tumors.

To provide a topical composition for photodynamic therapy, an obstructive dressing material, a kit using them, a material for photodynamic therapy, and a method for photodynamic therapy.SOLUTION: In order to promote infiltration of a topical composition containing 5-aminolevulinic acid (ALA) applied on a treatment site for photodynamic therapy, transepidermal water loss from the treatment site is suppressed to the minimum by covering the treatment site by a low-density polyethylene barrier before phototherapy after the topical composition is applied to the treatment site.SELECTED DRAWING: Figure 3

The present application relates to a kind of based on photodynamic therapy and radiotherapy and chemotherapy treatment stent and its preparation method.The present application includes stent body, stent body is mesh stent, mesh stent is provided with fixed frame, fixed frame includes the 4 support frames of cross arrangement, the cross point of 4 support frames is hinged connection.The present application is simple in structure, easy to make, convenient to use, can be made into different specifications and models, applicable to different tumor size, reduce the damage to normal tissue.

A compound including a general formula of D-L-A particularly with the structure of Formula (A). A nanoparticle of the compound of the present invention. A pharmaceutical composition including the compound of the present invention as an active ingredient and a pharmaceutically acceptable carrier. A method of treating a target tissue including administering to a patient in need thereof a compound of the present invention and administering to the target tissue radiation in an amount and of a wavelength effective to activate the compound. Also use of the compound of the present invention in preparation of a medicament for treating the target tissue by photodynamic therapy.

The present invention discloses a Nile blue dye and its preparation method, a preparation method of a packaging film based on the prepared dye, and the uses of the dye and the packaging film, belonging to the technical field of food monitoring and preservation. Based on the Nile blue parent structure, by introducing different heavy atoms at the meso position and introducing alkyl chains with different chain lengths at the amino position, photosensitive dyes with various pH responses and photodynamic therapy capabilities are constructed. By comparing various heavy atom substitution structures, Dye I and Dye II of the present invention are both sensitive to the pH of the solution to a certain extent, and the thio-dye packaging film and the seleno-dye packaging film both exhibit excellent antibacterial ability against Escherichia coli. The Dye I packaging film and the Dye II packaging film of the present invention both have excellent antibacterial activity against Escherichia coli. Both can achieve visual detection of the decay process of fresh shrimps and preservation of bananas.

The invention relates to an estrogen receptor alpha and fibroblast activating protein dual-targeting near-infrared fluorescent probe as well as a preparation method and medical application thereof. Specifically, the probe can be used as a fluorescence probe for developing cells co-expressing the estrogen receptor alpha and the fibroblast activating protein, and can also be used as a near-infrared fluorescence imaging probe for diagnosing benign / malignant lesions such as nodules / tumors co-expressing the estrogen receptor alpha and the fibroblast activating protein. The fluorescent probe can also be applied to research on related biological events in cells for co-expressing the estrogen receptor alpha and the fibroblast activating protein, and particularly can be applied to fluorescent navigation in tumor resection operations for co-expressing the estrogen receptor alpha and the fibroblast activating protein; particularly, the composition can be applied to the synergistic treatment of an anti-estrogen therapy and a photodynamic therapy on a diseased region which co-expresses the estrogen receptor alpha and the fibroblast activating protein.

The invention relates to the technical field of pharmaceutical preparations, in particular to composite condensing gel based on photodynamic therapy and a preparation method of the composite condensing gel. The preparation method of the composite condensate gel based on photodynamic therapy comprises the following steps: preparing a BSA (Bovine Serum Albumin) solution, uniformly mixing an inulin mother solution with the BSA solution, adjusting the pH value, heating in a water bath, cooling to room temperature, adding 5-aminolevulinic acid methyl ester hydrochloride into the condensate gel, adding an acid coagulant, diluting, and filling CO2 gas. According to the invention, CO2 is added into the composite condensation gel, so that the binding force of hemoglobin and O2 in tissues absorbing the composite condensation gel is reduced, and the concentration of free oxygen is improved, so that the composite condensation gel can transfer energy to surrounding oxygen and generate singlet oxygen with strong activity, thereby killing cancer cells and achieving the effect of improving the curative effect of photodynamic therapy.

The invention discloses a fibroin nanofiber-bismuth nanoparticle composite membrane and a preparation method thereof. The composite membrane is formed by compounding bismuth nanoparticles and fibroin nanofibers; the preparation method comprises the following steps: dispersing fibroin nanofibers in deionized water, heating and stirring until the fibroin nanofibers are dissolved, then adding bismuth nitrate powder, continuously stirring uniformly, and then adding a reducing agent for treatment until no bubble overflows, so that bismuth elementary substances grow on the fibroin nanofibers to obtain a bismuth-based fibroin nanofiber solution; and drying the bismuth-based fibroin nanofiber solution to obtain the bismuth-based fibroin nanofiber composite membrane. The obtained membrane product has excellent hydrophilicity, biodegradability, anti-inflammatory performance and antibacterial performance, is wide in application, can simulate an extracellular matrix structure, is used as an infectious skin wound dressing, can be combined with ion slow release / photo-thermal / photodynamic therapy to promote wound repair, and is safe and effective.

The present invention provides a dual-cell-targeted luminescent near-infrared fluorescent probe (E)-4-(4-(bis(4-(2-morpholinoethoxy)phenyl)amino)styryl)-1-(2-hydroxyethyl)quinolin-1-ammonium hexafluorophosphate (V), namely the fluorescent probe MoTQu. This dual-cell-targeted fluorescent probe contains a quinolinium cation moiety and targets mitochondria through electrostatic interaction with the electronegative mitochondrial membrane; the introduction of a weakly basic morpholine ring in the triphenylamine tail chain of this fluorescent probe targets lysosomes. At the same time, the preparation method and application of this probe are disclosed. The fluorescent probe of the present invention can not only light up different organelles in response to time, but also effectively improve the killing efficiency of cancer cells through the synergy of mitochondrial- and lysosome-targeted photodynamic therapy, and thus has a powerful effect on the ablation of photodynamic cancer cells.

The present invention provides an organic framework derivative phototherapeutic agent containing ruthenium and iron metal atoms for photothermal and photodynamic therapy, and a preparation method and application thereof. The preparation method of the phototherapeutic agent of the present invention includes the steps of: dissolving an iron salt, a ruthenium salt, a zinc salt and 2-methylimidazole in a solvent, stirring and reacting, and then centrifuging, washing and freeze-drying to obtain a precursor; the precursor is calcined to obtain the phototherapeutic agent. The phototherapeutic agent of the present invention has good structural stability, photothermal conversion characteristics and photosensitivity; not only has the property of converting light energy into heat energy, but also has the photosensitive property of converting light energy into kinetic energy and then generating reactive oxygen species; it can be effectively used for photothermal therapy and photodynamic therapy, and thus can be used as a phototherapeutic agent for the treatment of tumor cells.

The invention relates to the technical field of biology, in particular to a preparation method and application of a bionic nano regulator. The invention provides a bionic nano regulator. The nano regulator comprises a therapeutic drug; the therapeutic drug is prepared from Ce6 and SB525334. And extruding the bacterial outer membrane vesicles and the PLGA encapsulating the Ce6 and SB525334 medicines to obtain the bionic nano regulator. Under photodynamic therapy, the bionic nano regulator can be recognized, taken and delivered to tumor tissues by neutrophils. The bionic nano regulator can regulate and control polarization of neutrophil to an anti-tumor N1 type, promote the neutrophil to release neutrophil elastase, specifically kill tumor cells and reverse a tumor immunosuppression microenvironment. Under the combined treatment of photodynamic-immunotherapy, the biomimetic nano regulator obviously inhibits tumor growth and enhances the anti-tumor immune response of the body.

The present invention belongs to the field of photodynamic therapy technology, and more particularly to a BODIPY compound, a preparation method and its use as a photosensitizer. The present invention provides a series of novel small molecule compound photosensitizers with BODIPY dimer as basic skeleton and pure type I photodynamic. By the combined action of heavy atoms, 2,4,6-trimethylphenyl groups and limited structure R groups introduced at specific positions, the reactive oxygen species yield is increased, and the regulation of pure type I photosensitizer is carried out by regulating energy levels. Compared with common photosensitizer molecule indocyanine green (ICG), the reactive oxygen species yield is about 50 times that of ICG, has higher photothermal stability, can significantly increase the applicability of photodynamic agents, and the preparation method is simple, the reaction efficiency is high, the yield is high, and there is good industrial application prospect.

The present application relates to the technical field of cancer detection, in particular to a kind of tumor imaging guided photodynamic therapy reagent.The reagent is named ADS254BE / H2TPP NPs, which is composed of hydrophobic conjugated polymer ADS254BE and tetraphenylporphyrin H2TPP doped polymer microspheres, and the surface of the polymer microspheres also has PSMA modification layer.The reagent is prepared by uniformly mixing ADS254BE, H2TPP and PSMA solution, ultrasonic treatment, room temperature standing, and then evaporating solvent in the solution.The photodynamic diagnosis and treatment reagent provided by the present application shows bright red emission centered at 650nm, relatively large stoke shift, higher singlet oxygen generation rate, and good light stability and biocompatibility.The present application provides a feasible strategy for constructing new optical diagnosis and treatment integrated reagent, and is expected to promote the development of imaging guided photodynamic therapy.

The invention belongs to the technical field of biological medicines, and discloses a carbazole / benzo [cd] indole compound as well as a preparation method and application thereof. The carbazole / benzo [cd] indole compound has a structure as shown in a general formula I: # imgabs0 #, wherein R is one of NO2, NH2, Br and N (Ph) 2. The carbazole / benzo [cd] indole compound can generate active oxygen molecules after illumination, including superoxide anions and hydroxyl radicals, and can be applied to preparation of drugs for photodynamic therapy.

The present application relates to the field of pharmaceutical preparations, in particular to 5-amino-ketoglutaric acid metal complex, liposome and preparation method thereof. The 5-amino-ketoglutaric acid metal complex of the present application is formed by 5-amino-ketoglutaric acid and metal ion through coordination bond, and is a photosensitizer which can be used for photodynamic therapy of tumor. After ALA forms complex with metal ion, the reaction activity of amino and ketone groups in ALA molecule is reduced, the adverse influence of external environment on the molecule is delayed, and the stability is enhanced. Moreover, the co-aggregation of ALA and copper ion (or iron ion) to target tissue, the two means of ALA photodynamic therapy of tumor and copper (iron) ion promoting tumor cell apoptosis play drug efficacy, and have synergistic anti-tumor effect. The present application further provides liposome loaded with 5-amino-ketoglutaric acid metal complex, and preparation containing 5-amino-ketoglutaric acid metal complex or liposome.