The application discloses a fluorescent
coupling reaction low-
light exposure control incubation integrated
system, relates to the technical field of biological detection and experimental equipment, and comprises an operation dark box module, a sample incubation module, an imaging
assembly and a main control module. The operation dark box is combined with non-visible light imaging and hand action detection, realizes low-light blind operation
visualization and dynamic light adjustment, and reduces initial
light exposure. The sample incubation module initiatively creates a grading light shielding
system, uses local light shielding to timely stop loss of a
single sample, and uses overall light shielding to unify the dark field environment of subsequent reactions of all batches. In addition, the
system calculates accurate
exposure time through operation triggering and in-situ detection, combines multi-level threshold value quantization to identify the light bleaching degree of samples, and automatically determines to discard when the time is up. The application effectively solves the problems of uncontrollable
light exposure and uneven light receiving in the preparation and transfer process of large quantities of fluorescent samples, and guarantees the accuracy and high consistency of final fluorescent detection results.