The invention discloses an acer elegans
cross breeding method based on flowering
phase difference regulation and control, and belongs to the technical field of garden
plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering
phase difference is greater than or equal to 10 days and the
genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature
refrigeration and shading
blue light supplementing treatment on a female parent to
delay the flowering phase, and carrying out prolonged illumination,
red light supplementing and
hormone and
nutrient solution spraying on a male parent to advance the flowering phase; male parent
pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a
sucrose solution is sprayed to female parent stigmas before
pollination, and repeated
pollination is conducted through a directional
nozzle at 9-11 o'
clock every day; carrying out stratification treatment and temperature-difference temperature-control
germination on the
hybrid seeds, and carrying out light-temperature regulation and control
seedling culture after
sowing; the
molecular marker is used for verifying the hybridization authenticity and target traits,
phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the
caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.