The invention discloses a single-component UV delayed curing adhesive composition as well as a preparation method and an application method thereof, and belongs to the technical field of radiation curing adhesives. The adhesive composition comprises light-cured oligomer resin, an acrylate reactive diluent, epoxy resin, a curing agent, a photoinitiator and a free radical inhibitor, wherein the photoinitiator is formed by mixing a photobase generator and a photosensitizer. The preparation method of the UV curing adhesive composition is simple, the UV curing adhesive composition can be applied toadhesion between lightproof base materials, the adhesive applied to the surfaces of the base materials cannot be cured immediately after being irradiated by ultraviolet light, a certain operation time window can be provided to meet lap joint shaping between the base materials under different application scenes, and subsequently, sufficient bonding between the base materials can be achieved through post-curing. The adhesive composition is high in assembly efficiency, has relatively good bonding strength after being cured, and has a good application prospect in the fields of electronic components, space flight and aviation, automobile machinery, building materials and the like.