60 results about "Endothelial tissue" patented technology

A device is disclosed for the securement of dermal tissues. The device provides approximation and eversion of the tissue as well as the placement of a fixation element that bridges a wound. The fixation element may be produced in several fixed or dynamic configurations that may or may not alter the ability to engage tissue in response to stresses placed upon the fixation member post-deployment. The delivery device inserts the fastener percutaneously through the epidermis into the sub-dermal position without entering the wound margins with any part of the approximation portion of the delivery device.

This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including the skin, gastrointestinal tract, pulmonary epithelium, and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidino or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length.

A compact retrievable blood clot filter has a filter section, a releasable lock and an alignment section connected to the filter section. Alignment ribs of the alignment section have releasable upstream ends that are locked to the filter by the releasable lock. The releasable upstream ends of the alignment ribs are capable of being released from the releasable lock so that during retrieval of the filter, the alignment ribs can slide through the endothelial tissue that may have grown around the alignment ribs.

The present invention is drawn to methods and compounds for photodynamic therapy (PDT) of a target tissue or compositions in a mammalian subject, using a light source that preferably transmits light to a treatment site transcutaneously. The method provides for administering to the subject a therapeutically effective amount of a targeted substance, which is either a targeted photosensitizing agent, or a photosensitizing agent delivery system, or a targeted prodrug. This targeted substance preferably selectively binds to the target tissue. Light at a wavelength or waveband corresponding to that which is absorbed by the targeted substance is then administered. The light intensity is relatively low, but a high total fluence is employed to ensure the activation of the targeted photosensitizing agent or targeted prodrug product. Transcutaneous PDT is useful in the treatment of specifically selected target tissues, such as vascular endothelial tissue, the abnormal vascular walls of tumors, solid tumors of the head and neck, tumors of the gastrointestinal tract, tumors of the liver, tumors of the breast, tumors of the prostate, tumors of the lung, nonsolid tumors, malignant cells of the hematopoietic and lymphoid tissue and other lesions in the vascular system or bone marrow, and tissue or cells related to autoimmune and inflammatory disease.

This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including the skin, gastrointestinal tract, pulmonary epithelium, ocular tissues and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidino or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length.

The present invention provides compositions and methods for enhancing transport of biologically active compounds across biological membranes and across and into animal epithelial or endothelial tissues. The composition includes a biologically active agent and a transport moiety. The transport moiety includes a structure selected from the group consisting of (ZYZ)nZ, (ZY)nZ, (ZYY)nZ and (ZYYY)nZ. Subunit “Z” is L-arginine or D-arginine, and subunit “Y” is an amino acid that does not comprise an amidino or guanidino moiety. Subscript “n” is an integer ranging from 2 to 10. The method for enhancing transport involves the administration of the aforementioned composition.

The present invention is drawn to methods and compounds for transcutaneous photodynamic therapy ("PDT") of a target tissue or compositions in a mammalian subject, which includes administering to the subject a therapeutically effective amount of a photosensitizing agent or a photosensitizing agent delivery system or a prodrug where the photosensitizing agent or photosensitizing agent delivery system or prodrug selectively binds to the target tissue; and irradiating at least a portion of the subject with light at a wavelength absorbed by the photosensitizing agent or if prodrug, by a prodrug product thereof where the light is provided by a light source, and where the irradiation is at low fluence rate that results in the activation of the photosensitizing agent or prodrug product. These methods of transcutaneous PDT are useful in the treatment of specifically selected target tissues, such as: vascular endothelial tissue abnormal vascular wall of tumors; tumors of the head and neck; tumors of the gastrointestinal tract; tumors of the liver; tumors of the esophopharyngeal; tumors of the lung; lymphoid tissue; lesions in the vascular system; hone marrow and tissue related to autoimmune disease.

The present invention provides targeted delivery systems to deliver pharmaceuticals to irradiated tissue comprising a biomolecule carrier, a targeting moiety to cellular adhesion molecules and a pharmaceutical. The present invention also provides methods of selectively targeting endothelial tissue for delivery of a pharmaceutical thereto and of treating a pathophysiological state in an individual using the targeted delivery systems disclosed herein. Further provided is a method of optimizing an immunoliposome for specific targeting of a pharmaceutical encapsulated therein to irradiated tissue by selecting a liposome that has a greater rate of adhesion to the irradiated tissue than a rate of uptake by the reticuloendothelial system.

A removable blood clot filter includes anchor members with a sharp edge configured so that when the filter is removed the sharp edge aids in passing the anchor members through endothelial tissue. The filter may also include a sharp edge on locator members configured to aid pulling the locator members away from the endothelial tissue.

Serum compositions for application to endothelial tissue are described which contain an amniotic fluid extract in combination with embryonic stem cells. Formulations containing the serum composition are also described. The serum compositions and formulations may be used to treat conditions of the mouth such as dry mouth and mouth sores.

A device transfers donor endothelial cells to the cornea of a recipient without injuring the cells or the cornea during the transfer process. The device transfers living endothelial cells from a donor to a recipient with minimal or no trauma. The device includes a handle, an irrigation tube lengthwise within the handle, a button upon the handle concentric with the irrigation tube, a tapered nose opposite the button, a forward tube extending outwardly from the nose, and a platform joined to the irrigation tube. The forward tube has an elliptical cross section and two beveled features. The platform has two wings that curl gently around donor tissue upon retracting the platform into the forward tube. The handle has a groove for a stem of a knob that surgeon presses and pulls to move the platform.

The invention provides an immortalized dairy cow rumen endothelial cell line and a construction method thereof. The construction method comprises the following steps: (a) cutting, washing, and digesting collected dairy cow rumen endothelial tissues in a culture medium, and carrying out primary culture to obtain rumen endothelial cells; step (b) incubating rumen endothelial cells obtained in the step (a) with a viral liquid containing an SV40T antigen gene to obtain infected rumen endothelial cells; and step (c) culturing the infected rumen endothelial cells obtained in the step (b) to obtain the immortalized dairy cow rumen endothelial cell line. The provided immortalized dairy cow rumen endothelial cell line can provide a test cell model for the research on physiological regulation and nutrient absorption mechanism of dairy cow rumen. The culture method is simple, the growth speed is quick, and the construction method can obtain BRECs, which have physiological functions and can carryout continuous passage.

The invention discloses a developable sealed type woven body implantation material, which comprises a woven mesh, a sealing film and a developing layer and is characterized in that the woven mesh is a single-layer or double-layer nickel-titanium alloy mesh, the developing layer is one layer of coated developing materials, the sealing film is sewed into any one layer of the single-layer or double-layer nickel-titanium alloy mesh, then, an endothelial cell growth promotion layer is coated at the outer surface of the sealing film, and the developing layer is arranged among or outside any layer of the woven mesh, the sealing film and the endothelial cell growth promotion layer. The conveying device comprises an outer sheath tube (32), an inner sheath tube (31) and a conveying guide wire (30), wherein the inner sheath tube (31) is sheathed in the outer sheath tube (32) and is glidingly connected with the outer sheath tube (32). The woven body implantation material is in a self-expansion type, the integral developing can be realized, the sealing film can seal and isolate the hemangioma, the endothelial cell growth promotion layer promotes the growth of endothelial cells in the surface of the woven body implantation material, the woven body implantation material is coated inside endothelial tissues of blood vessels, and the risk of recent and future thrombus generation is reduced.

The present invention discloses an artificial substitute unit. It can be implanted into human body and can be used as temporary substitute for retaining specific tissue function. Said substitute includes a peripheral body, a hollow supporting frame placed in said peripheral body interior, intracavity filled cells placed in the inner cavity of said supporting frame and filler and functional cells and nutrient matrix body placed between said supporting frame and peripheral body, on the wall surfaces of the described supporting frame and peripheral body several through microporous channels are uniformly distributed, and the described intracavity filled cells can be induced and differentiated into endothelial tissue cells, and the extracavity filled cells can be changed according to the application direction of artificial substitute unit, and can be induced and differentiated into required functional cells, such as liver cells, pancreatic island beta cells, adrenal cortex cells and fat cells, etc.

The invention discloses a biodegradable intravascular stent with a composite coating. The biodegradable intravascular stent comprises a stent body and a multifunctional composite layer, wherein the multifunctional composite layer sequentially comprises a porous transition layer, a dopamine polymer layer, an anticoagulant drug layer, an endothelial regeneration promotion cell factor layer and a copper ion sterilization layer from inside to outside; the porous transition layer is prepared from the following raw materials in parts by weight: 20-30 parts of nano-silica, 0.1-0.2 part of vanillic aldehyde and 0.1-0.2 part of thymol. The biodegradable intravascular stent is scouring-resistant and stable, and has strong ability of capturing endothelial tissue cells in vitro, thus achieving the aims of well inhibiting thrombosis and reducing restenosis; therefore, the biodegradable intravascular stent has important clinical application value.

The present invention relates to delivery of biologically active molecules to cells. Specifically, the invention relates to polycationic compositions, polymers and methods for delivering nucleic acids, polynucleotides, and oligonucleotides such RNA, DNA and analogs thereof, including short interfering RNA (siRNA), ribozymes, and antisense, or peptides, polypeptides, proteins, antibodies, hormones and small molecules, to cells by facilitating transport across cellular membranes epithelial tissues and endothelial tissues. The compositions and methods of the invention are useful in therapeutic, research, and diagnostic applications that rely upon the efficient transfer of biologically active molecules into cells, tissues, and organs.

A cornea matrix transplant for providing proper carrier used for cornea transplantation is prepared from the pharmacologically acceptable biodegradable material and fibroblasts through mixing to inoculate said fibroblasts onto said biodegradable material.

A compact retrievable blood clot filter has a filter section, a releasable lock and an alignment section connected to the filter section. Alignment ribs of the alignment section have releasable upstream ends that are locked to the filter by the releasable lock. The releasable upstream ends of the alignment ribs are capable of being released from the releasable lock so that during retrieval of the filter, the alignment ribs can slide through the endothelial tissue that may have grown around the alignment ribs.

If pathogen factors such as meningococcal NMB 1870 or flaviviral NS1 are able to sequester factor H in the blood then its inhibitory effect on complement may be disturbed, thereby permitting C3 to initiate a dramatic attack on host endothelial tissue. In combination with a strong inflammatory response, this attack can result in sever damage to the endothelium, with resulting hemorrhagic syndrome. Blocking the interaction between pathogen factors and factor H may thus be used to treat and / or prevent these pathogen-induced hemorrhagic syndromes. The interaction may, for instance, be blocked by antibodies, either delivered endogenously (passive immunisation) or produced by a patient's immune system (active immunisation).

The invention provides a drug-eluting coronary artery stent and a method for preparing the same. The method comprises the following steps: providing a basal body for the coronary artery stent; providing a micro arc oxidation treatment device comprising an electrolyte tank; adding an electrolyte into the electrolyte tank and stirring the electrolyte evenly, wherein the formulation of the electrolyte comprises that calcium acetate (CH3COO)2Ca.H2O of which the concentration is between 0.2 and 0.6 mol per liter and sodium dihydrogen phosphate NaH2PO4 of which the concentration is between 0.02 and 0.06 mol per liter are added to 400 milliliters of distilled water respectively; and putting the basal body of the coronary artery stent into the electrolyte, starting the micro arc oxidation treatment device to run for 1 to 12 minutes to obtain the basal body of the coronary artery stent, and then assembling the basal body of the coronary artery stent with microspheres for encapsulating drugs so as to obtain the drug-eluting coronary artery stent. While the drug-eluting coronary artery stent slowly releases the drugs, the drug-eluting coronary artery stent promotes the complete blood vessel endothelialization on the surface of the implanted coronary artery stent for endothelial tissues of normal coronary artery blood vessels to grow on the surface of the implanted stent.

The invention discloses a thrombus filter capable of realizing delayed recovery and a preparation method of the thrombus filter and particularly discloses a blood compatible coating. The blood compatible coating comprises a polyacrylic acid layer and a polyethylene glycol layer, and 2-methacryloyloxyethyl phosphorylcholine is covalently linked with the surface of the polyethylene glycol layer. Theinvention further discloses medical apparatus such as the thrombus filter coated with the blood compatible coating. According to the technical scheme, thrombogenesis and endothelial tissue growth canbe better inhibited. The whole surface coating is adjustable in thickness and surface grafting amount and can well bear stress and strain actions in apparatus assembly and release processes, molecules are firmly combined without falling off, and mechanical properties of the apparatus are not affected.

The invention discloses a method of building specificity endothelial cell growth promoting anticoagulant surface, belongs to the technical field of biomaterial surface modification. The method comprises the steps of 1, preparation of poly-dopamine of biomaterial surface; 2, micro-contact printing of fixed bi-amino glycol of biomaterial surface; 3, fixing of chitosan and heparin of biomaterial surface; 4, fixing of REDV of biomaterial surface; the building method is beneficial to the control of adhesion growth behavior of endothelial cells and the achieving of the complete healing of endothelial tissue; when the anticoagulant surface is used for the endovascular implantation materials or surface modification of equipment, excellent endothelial cell growth promoting property and anticoagulant property can be attached at the same time; due to the formation of a solid complex coating by auto-polymerization of dopamine on all solid material surfaces, and the formed coating has a strong ability to react with amino-containing substances, the building method can be used for the surface modification of almost all of biomaterials and for the building of anticoagulant surface for promoting the growth of specificity endothelial cells.

A removable blood clot filter includes anchor members with a sharp edge configured so that when the filter is removed the sharp edge aids in passing the anchor members through endothelial tissue. The filter may also include a sharp edge on locator members configured to aid pulling the locator members away from the endothelial tissue.

A novel renal precursor cell is identified from kidney tissue. The cell is multipotent capable of forming renal epithelial and endothelial tissues. The cell can be amplified in culture and maintains stemness over multiple passages. This cell fulfills a major unmet need as a cell-based source for kidney regeneration or repair. Treatment of kidney diseases and disorders (e.g., glomerularpathies and acute tubular necrosis) may be improved thereby.

The invention discloses a zwitterionic polypeptide, a derivative of the zwitterionic polypeptide and a nano-drug based on the zwitterionic polypeptide. Nano-drugs can be prepared on the basis of the zwitterionic polypeptide or derivatives thereof, the secondary structure of the zwitterionic polypeptide has excellent conversion capability before and after drug release, and release of the drugs in cells can be accelerated. The obtained nano-drug can be used for tumor targeted therapy; unexpected tumor targeting performance is obtained; and the nano-drug has excellent blood compatibility, has thecapability of avoiding immune recognition, tumor targeting, intracellular transformation and entering into cell nuclei, can reduce the distribution in liver, kidney, spleen, lung, heart and other organs rich in reticuloendothelial tissues, and finally realize the capability of efficiently inhibiting tumor growth and the in-vivo low-toxicity targeting tumor treatment effect.

The invention involves a model of naked mouse endothelial ancestral cell which urges the new born of blood vessel. The endothelial ancestral cell after being mixed with the base jel is injected into the endothelial tissue of naked mouse. Give medicine through routine way to naked mouse to effect the differentiation of the endothelial ancestral cell inside the base-jel in naked mouse; the endothelial ancestral cell differentiates in the base jel into blood vessel ebdothelial cell with remarkable structure characteristic to growth blood vessel of network grid form. Then take out the base jel. Advantages: low cost and short period.

The invention discloses an electrostatic spinning receiving device for preparing an axial orientation tubular tissue engineering material, and belongs to the field of tissue engineering material preparation. The electrostatic spinning receiving device comprises an electrostatic spinning spinneret, wherein the receiving device is arranged below the spinneret; the receiving device comprises one pairof receiving polar plates which are vertically arranged and are mutually in parallel, and a scroll; the scroll is vertical to the two receiving polar plates and is overlapped on the upper edges of the two receiving polar plates; the receiving device also comprises a pushing mechanism which pushes the scroll to carry out back and forth movement along the horizontal direction of the scroll; the scroll is also connected with a rotation mechanism. A nanometer fiber tubular tissue engineering material prepared with the device is high in a fiber axial orientation degree, and is used as an artificial blood vessel to be favorable for endothelial cells to grow along the axial orientation and form endothelial tissues which are the same with a true blood vessel.

The present invention provides compositions and methods for enhancing transport of biologically active compounds across biological membranes and across and into animal epithelial or endothelial tissues. The composition includes a biologically active agent and a transport moiety. The transport moiety includes a structure selected from the group consisting of (ZYZ)nZ, (ZY)nZ, (ZYY)nZ and (ZYYY)nZ. Subunit “Z” is L-arginine or D-arginine, and subunit “Y” is an amino acid that does not comprise an amidino or guanidino moiety. Subscript “n” is an integer ranging from 2 to 10. The method for enhancing transport involves the administration of the aforementioned composition.