49 results about "2-methacryloyloxyethyl phosphorylcholine" patented technology

The invention aims to provide a reagent for assaying an antiphospholipid antibody which is excellent in long-term storage stability and capable of diagnosing syphilis infection or the like and a reagent for assaying an anti-Treponema pallidum antibody which is capable of diagnosing syphilis infection with high accuracy by preventing the occurrence of serum interference. The invention is directed to the reagent for assaying an antiphospholipid antibody to be used for diagnosing syphilis infection comprising an insoluble carrier carrying an antiphospholipid antigen and a copolymer with a segment derived from 2-methacryloyloxyethyl phosphorylcholine and a segment derived from a hydrophilic monomer.

The invention discloses a preparation method of a hyperbranched polyimide anti-coagulant antibacterial material, and belongs to the field of chemical materials. The surface of amino-terminated hyperbranched polyimide is subjected to graft modification by 2-methacryloyloxyethyl phosphorylcholine to form a hyperbranched polyimide film, and the biocompatibility, anticoagulant property and antibacterial property of the prepared HBPI-MPC film are good.

The invention relates to a phosphorylcholine-containing high-strength polyurethane hydrogel and a preparation method thereof, and the hydrogel is prepared from a double-bond-terminated polyurethane segmented copolymer, 2-methacryloyloxyethyl phosphorylcholine and an alkene copolymerization monomer through free radical copolymerization. The hydrogel is prepared by dissolving the double-bond-terminated polyurethane segmented copolymer, 2-methacryloyloxyethyl phosphorylcholine, the alkene copolymerization monomer and an initiator in a solvent, and performing ultraviolet-initiated free radical polymerization reaction at room temperature. The preparation method is simple, the raw material source is wide, and the prepared hydrogel is good in biocompatibility, also possesses extremely strong compression resistance and tensile resistance, can be used as a tissue engineering restoration skeleton material and the like, and possesses obvious application prospect in the field of biomedical materials.

The invention discloses a method for grafting 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on a titanium-base material surface. A layer of middle bridging groups, namely organic phosphate or phosphate monoester which respectively forms chemical bonding with the titanium-base material surface and the MPC polymer is prepared on the titanium-base material surface and the MPC polymer by a self-assembled monolayer method; and the MPC polymer is grafted on the titanium-base material surface by a surface-initiated atom transfer radical polymerization method. In the prepared simulative functionalized titanium-base material of the method, the MPC polymer on the surface of the simulative functionalized titanium-base material has strong bonding force with the titanium-base material and has better histocompatibility and better blood compatibility. The invention has simple process and is easy to realize.

The invention discloses a hydroxy propyl cellulose graft copolymer, a preparation method and an application thereof. The method comprises the following steps: in an acid aqueous solution, utilizing cerium salt to trigger hydroxy propyl cellulose to be grated with poly(2-methacryloyloxyethyl phosphorylcholine) (MPC), thereby introducing the MPC with an imitated biomembrane structure into the main chain of the hydroxy propyl cellulose; controlling reaction time to obtain anticoagulant hydroxy propyl cellulose derivatives with different grafting ratios; and utilizing an electrostatic spinning technology and adjusting solvent and relevant spinning condition parameters to obtain nanoscale cellulose fiber. The nanoscale HPC-g-PMPC fiber prepared in the invention has large specific surface area and biological functionality, thereby being capable of being applied to the biological medical fields of preparing anticoagulation non-woven fabrics, tissue engineering support materials, wound clad materials, medicine controlled release films and the like.

The present invention provides an agglutination immunoassay, wherein the agglutination of insoluble carrier particles such as latex are stabilized and uniformized to give good reproducibility, and a reagent therefor. In the agglutination immunoassay which comprises allowing an antigenic substance in a sample to bind to insoluble carrier particles carrying substantially neither antigens nor antibodies thereon, and allowing an antibody or an antibody complex which reacts specifically to the antigenic substance to bind to the antigenic substance to give a selective agglutination of the insoluble carrier particles, a homopolymer prepared by polymerization of a monomer such as 2-methacryloyloxyethyl phosphorylcholine having a phosphorylcholine group and a vinyl group, or a copolymer prepared by polymerization of a monomer having a phosphorylcholine group and a vinyl group, with a monomer having a vinyl group such as n-butyl methacrylate is used.

The invention discloses a coronary artery stent with anticoagulant effect and a preparation method thereof. The hyperbranched star-shaped amphiphilic SPLA-b-PMPC polymer nano particle coating is arranged on the support. Dissolve the treated stent in an organic solvent of hyperbranched star-shaped amphiphilic SPLA-b-PMPC polymer nanoparticles to prepare a coating solution; then take out the stent; place the stent on a centrifuge device and load it into a centrifuge tube , centrifuged, and dried under reduced pressure at room temperature to complete the coating. The 2-methacryloyloxyethylphosphorylcholine contained in the coating liquid of this coronary artery stent has little interaction with blood components, which can effectively inhibit blood coagulation and complement activation, and reduce protein adsorption , Inhibit the denaturation of adsorbed protein and platelet adhesion, can inhibit cell adsorption and inflammatory response, has good blood and tissue compatibility, and has a good anticoagulant effect, and has good application in cardiovascular and cerebrovascular diseases prospect.

The invention discloses a coronary stent with anti-coagulation and vascular endothelial cell adhesion acceleration effects and a preparation method thereof. The method comprises the following steps: soaking the stent into a dopamine and lysine solution to bath; soaking in an N-(3-aminopropyl) methylacrylate solution to carry out pre-treatment, and then dissolving the processed stent into a 2-methacryloyloxyethyl phosphorylcholine solution; adding tetramethylethylenediamine, N,N-methylene diacrylamine and ammonium persulfate to the solution to react; soaking into an arginine-glycine-aspartic acid (RGD) peptide solution, adding tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) to react, so as to prepare a coating. Protein adsorption, denaturation of adsorption protein and platelet adhesiveness can be reduced and inhibited by a 2-methacryloyloxyethyl phosphorylcholine (MPC) part contained in the coating of the coronary stent, inflammatory response is inhibited, adhesion to the stent caused by vascular endothelial cells can be accelerated by the contained RGD part, the coronary stent has good biocompatibility, the stent better exists inside a body to play the function, and the coronary stent has good application prospect in the aspect of cardiovascular and cerebrovascular diseases.

The invention provides a polylactic acid-bacterial cellulose composite material and a preparation method thereof. The composite material comprises polylactic acid, bacterial cellulose, graphene, 2-methacryloyloxyethyl phosphorylcholine, proanthocyanidins, polyethylene glycol, calcium chloride, a lubricant and an assistant. The preparation method comprises the following steps: drying polylactic acid; carrying out ultrafine crushing on the dried polylactic acid, the bacterial cellulose and graphene, and putting the crushed material in ethanol to prepare a dispersion solution; and carrying out room temperature volatilization on the dispersion solution to remove ethanol, uniformly mixing the ethanol removed dispersion with residual raw materials, adding the obtained mixture to a co-rotating twin-screw provided extruder, carrying out extrusion granulation, granulating through a pelleter, and drying until the mass is constant. Compared with polylactic acid-graphene composite materials, the polylactic acid-bacterial cellulose composite material prepared in the invention has the advantages of excellent mechanical performances, high tensile strength and bending strength, substantial reduction of the water absorption rate, and suitableness for processing application.

Disclosed is a respiration-assisting tube whereby tissue damages in vivo can be prevented and, as a result, inflammatory reactions and infections can be avoided. The respiration-assisting tube is developed based on the finding that adhesion of cells to a respiration-assisting tube can be inhibited by coating the respiration-assisting tube with a polymer containing 2-methacryloyloxyethyl phosphorylcholine (MPC). Also, the respiration-assisting tube is developed based on the finding that, by coating a respiration-assisting tube with a polymer containing MPC, mucosa peeling and tissue damages, which occur after using the respiration-assisting tube, can be prevented and, as a result, inflammatory reactions can be avoided.

The invention discloses a viscosity-reducing type polycarboxylic acid water reducing agent preparation method, which comprises: (1) preparing an esterification monomer; (2) carrying out a copolymerization reaction; and (3) carrying out a neutralization reaction. According to the present invention, the viscosity-reducing type polycarboxylic acid water reducing agent is prepared by co-polymerizing 2-allyloxyethanol, a blend, VPEG, 2-methacryloyloxyethyl phosphorylcholine and an unsaturated acid, such that the phosphate radical, the carboxylate radical, the quaternary ammonium salt and the estergroup are introduced into the molecular structure of the polymer while the polymer structure is lightly cross-linked, wherein the product obtained by the esterification of the single carboxy in 2-phosphonobutane-1,2,4-tricarboxylicacid prepared by a 2-phosphonobutane-1,2,4-tricarboxylicacid esterification reaction, the product obtained by the esterification of the two carboxy in a small amount of2-phosphonobutane-1,2,4-tricarboxylicacid and the product obtained by the esterification of the three carboxy in a small amount of 2-phosphonobutane-1,2,4-tricarboxylicacid form the blend; and the prepared viscosity-reducing type polycarboxylic acid water reducing agent has advantages of viscosity reducing, water reducing, slump retaining and mud resistance, and can solve the problems of high viscosity, poor workability and rapid loss due to the high mud content in the current concrete raw materials.

The invention discloses a thrombus filter capable of realizing delayed recovery and a preparation method of the thrombus filter and particularly discloses a blood compatible coating. The blood compatible coating comprises a polyacrylic acid layer and a polyethylene glycol layer, and 2-methacryloyloxyethyl phosphorylcholine is covalently linked with the surface of the polyethylene glycol layer. Theinvention further discloses medical apparatus such as the thrombus filter coated with the blood compatible coating. According to the technical scheme, thrombogenesis and endothelial tissue growth canbe better inhibited. The whole surface coating is adjustable in thickness and surface grafting amount and can well bear stress and strain actions in apparatus assembly and release processes, molecules are firmly combined without falling off, and mechanical properties of the apparatus are not affected.

The invention discloses a filter membrane for removing low density lipoproteins, and a preparation method thereof. The filter membrane comprises, by weight, 10-25 parts of amphiphilic chitosan, 20-35parts of sulfonic group-modified cellulose acetate, 2-6 parts of poly(2-methacryloyloxyethyl phosphorylcholine) and 1-4 parts of sodium oleate. The filter membrane can effectively reduce the LDL level in the blood, has a good filtering effect on VLDL, TC and TG, has few influences on the level of beneficial proteins in the blood, such as high density lipoproteins, albumins and immunoglobulin IgG,is suitable for purifying the blood of clinical hyperlipidemia patients, can also be applied to the finishing industry of blood products, and can be combined with relevant separation auxiliary devices to recover useful plasma from the waste human plasma that does not meet the blood transfusion standards and produce blood products with high added values.

It is an object of the present invention to provide a reagent for assaying anti-phospholipid antibodies, which is excellent in long-term storage stability and enables an accurate diagnosis of syphilitic infection, and a reagent for assaying anti-Treponema pallidum antibodies, which enables an accurate diagnosis of syphilitic infection by preventing an occurrence of serum interference.The present invention is a reagent for assaying anti-phospholipid antibodies used for diagnosing syphilitic infection, which contains an insoluble carrier supporting an anti-phospholipid antigen thereon and a copolymer having a segment derived from 2-methacryloyloxyethyl phosphorylcholine and having a segment derived from a hydrophilic monomer.

The invention relates to the technical field of medicines, in particular to application of a nanoparticle containing orlistat in preparing an anti-hepatitis B virus (HBV) medicine. The nanoparticle isprepared from the orlistat and a copolymer chosen from a vitamin A methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer or a vitamin E methacrylate-2-methacryloyloxyethyl phosphorylcholinecopolymer or a vitamin D2 methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer. The encapsulation efficiency of the nanoparticle is 82.67-93.83%; animal test shows that after oral administration of the nanoparticle, the blood drug concentration is higher, and the sustained-release effect is good; an in-vitro test result shows that the inhibition ratio of extracellular HBV DNA replication is used as an index, and the activity of the nanoparticle for resisting HBV infection is significantly higher than those of the orlistat and a contrast nanoparticle prepared by adopting PMB30W as acarrier.

The invention relates to the technical field of medicines, and in particular to an application of nano-microsphere containing orlistat in preparing antineoplastic drugs. The nano-microsphere consistsof the orlistat as well as any copolymer which is selected from a vitamin A methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer, a vitamin E methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer and a vitamin D methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer. Based upon in vitro test results, it is indicated that antineoplastic activity of the nano-microsphereprovided by the invention on human prostate cancer cells DU145, human melanoma cells A375 and human pancreatic cancer cell lines bxpc-3 is obviously higher than that caused by independent administration of the orlistat, and the antineoplastic activity is better than that of control nano-microsphere which is prepared by taking PMB30 as a carrier as well as nano-microsphere which contains the orlistat and polyethylene glycol-polycaprolactone.

The invention discloses a preparation method for a biodegradable high anti-bacterial anti-mold agent, and belongs to the technical field of the environmental protection preparation. The method comprises the following steps: according to the features of kieselguhr, such as high porosity and high liquid adsorption rate, using the kieselguhr as a carrier of the anti-mold agent, modifying kieselguhr powder by a titanate coupling agent, and extracting chitosan with the good biocompatibility, the biodegradability and the better anti-bacterial anti-corrosion property from shrimp shells and crab shells in acidity and alkaline reaction processes successively, grafting 2-methacryloyloxyethyl phosphorylcholine and sodium ethylenesulphonate on the chitosan, forming a derivative with the amphoteric iron characteristic, high molecular weight and water solubility, continuously reacting to generate quaternary ammonium salt in the high temperature and high pressure through dodecyl dimethylamine, isopropanol, sodium hydroxide solution and deionized water and the protection of chloromethane, enabling the quaternary ammonium salt to hybrid-modify with trimethylchlorosilane and a hydrochloric acid, andfurther improving the anti-bacterial property of the anti-mold agent. The anti-mold agent has the extensive using prospect.

Provided are a composition for a polymerase reaction, containing a nucleic acid polymerase and a 2-methacryloyloxyethyl phosphorylcholine (MPC)-containing zwitterionic copolymer detergent, a tube for a polymerase reaction, and a kit for a polymerase reaction. The stability of the composition for a polymerase reaction can be improved and the reliability of the results of polymerase reaction such as nucleic acid polymerization or amplification can be improved.

The invention relates to the technical field of medicine, in particular to an orlistat nanosphere and application of the same in preparation of obesity treatment drugs. The nanosphere comprises orlistat and a copolymer, wherein the copolymer is selected from one of a vitamin-A methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer, a vitamin-E methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer or a vitamin-D2 methacrylate-2-methacryloyloxyethyl phosphorylcholine copolymer. The encapsulation efficiency of the nanosphere is 82.67-93.83%. Animal experiments show that the vitamin absorption level of rats after the nanosphere is taken by the rats orally is obviously higher than the vitamin absorption level of rats after the orlistat and vitamins are taken by the rats orally at the same time (at the interval of two hours), and is similar to the vitamin absorption level of rats after the vitamins are taken by the rats individually and orally under fasting, so that deficiency of fat-soluble vitamin due to oral administration of the orlistat can be make up effectively, and safety weight loss can be realized.

The invention discloses a biodegradable composite material containing poly benzyl glutamate, and the preparation method. The method comprises three parts, namely, the synthesis of gamma-benzyl-L-glutamate N-carboxyl anhydride, the synthesis of end-allyl-poly benzyl glutamate, and the preparation of composite material. The synthesis of end-allyl-poly benzyl glutamate takes the gamma-benzyl-L-glutamate N-carboxyl anhydride as the monomer and takes diallyl amine as the initiator to prepare the end-allyl-poly benzyl glutamate through reaction in methylene chloride solution; through UV cross linking, polyethylene glycol dimethacrylate or 2-methacryloyloxyethyl phosphorylcholine with good hydrophilicity, biocompatibility and blood compatibility is introduced into the poly benzyl glutamate to prepare the biodegradable composite material containing poly benzyl glutamate. The composite material can be used for tissue repair in clinical medicine and has the advantages of simple preparation process and equipment, which has good application prospect and scientific significance.

The invention provides a preparation method for an anode device of a microbial fuel cell. The preparation method comprises the following steps of dissolving methacrylic acid in dichloromethane; adding dicyclohexylcarbodiimide and alkylamine osmium bipyridine chlorate for reaction; drying an organic phase to obtain a monomer Y after washing; dissolving 2-methacryloyloxyethyl phosphorylcholine, 2-butyl methacrylate, 4-vinylbenzene boronic acid and the monomer Y in absolute ethyl alcohol; adding azodiisobutyronitrile for reaction; depositing a product in a chloroform / ethyl ether mixed solvent, dissolving the product in deionized water, and carrying out reduced pressure distillation to obtain a copolymer PMBVY; mixing the copolymer PMBVY and a culture solution loaded with Shewanella to obtain a constituent I; mixing polyvinyl alcohol and a sterile culture solution to obtain a constituent II; placing an electrode in a die; adding the constituents I and II in same volume; uniformly mixing the constituents I and II until a sol is cured; and combing the electrode, an electrode plate, a substrate and a filter net to obtain the anode device. By the method, materials are recycled, and the anode device is convenient to maintain.

The invention provides a synthetic method of artificial cytomembrane main ingredient 2-methacryloyloxy ethyl phosphorylcholine. The synthetic method comprises the following steps: (1) dissolving phosphorus oxychloride in acetonitrile, adding triethylamine at 0 to 10 DEG C, dropwise adding 2,3-dibromo-2-hydroxyethyl methacrylate, reacting at -10 to 0 DEG C, adding triethylamine and choline chloride to facilitate the reaction at -10 to 0 DEG C, and obtaining bromophosphorylcholine after the reaction is ended; and (2) dissolving the bromophosphorylcholine obtained in the step (1) in ethanol, adding zinc powder, severely stirring, and obtaining 2-methacryloyloxyethyl phosphorylcholine. By adopting the synthetic method, a synthetic route of the original 2-methacryloyloxyethyl phosphorylcholine is simplified, no treatment is needed after the purification during the synthetic route, and the next operation can be directly carried out; severe conditions in the synthetic route are avoided, high-end equipment is not needed, uncontrollable factors during the synthetic route are reduced; raw materials are easy to obtain, the price is low, the cost is reduced, the use of solvents and raw materials with large toxicity is avoided, and the green synthesis is realized; and a foundation is set for the industrialization of the 2-methacryloyloxyethyl phosphorylcholine.

The invention relates to a hydrogel material with water content of 50%-60% and a preparation method thereof. The hydrogel is prepared by the following method: the hydrogel is prepared by the following raw materials, by mass percent: 79%-94$ of a hydrophilic comonomer hydroxyethyl methylacrylate, 5%-20% of a hydrophilic substance 2-methacryloyloxyethyl phosphorylcholine, 0.2%-5% of a crosslinking agent ethylene dimethacrylate, and 0.1%-2% of an initiator azodiisobutyronitrile; the raw materials are mixed and uniformly stirred; the raw materials are injected into a mould, and the reaction mixture is solidified by a method of heat initiation; and demoulding is carried out, and the unreacted monomers or oligomers are removed to obtain the hydrogel material with the water content of 50%-60%. Compared with the prior art, the hydrogel material with the water content of 50%-60% and the preparation method thereof have the advantages of simple preparation method and easy operation.

The invention relates to a hyperbranched star polylactic acid-poly(2-methacryloyloxyethyl phosphorylcholine) block polymer nanoparticle prepared by a volatilization method and a method. The method comprises the following steps: adding 0.01 to 100mg of hyperbranched star polylactic acid-poly(2-methacryloyloxyethyl phosphorylcholine) block polymer into a test tube, and adding 1 to 10ml of acetone / water mixed solvent into the test tube, wherein the volume ratio of the acetone to the water is 1:(0.1-10); adding 1 to 100ml of deionized water and a magnetic stir bar into a beaker, stirring, sucking the previously prepared acetone-aqueous solution of SPLA-b-PMPC by use of a disposable injector, and injecting into the beaker once; and volatizing the solvent at normal temperature to obtain the product. The nanoparticles are structurally spherical and uniformly dispersed, and have the particle diameter from 50 to 80nm and the critical micelle concentration from 9.94*10<-4> mg / ml.

Provided is a lubricity-imparting agent that can impart durable lubricity (in particular, lubricity at the time of wetting) to a substrate. It has been found that a lubricity-imparting agent including a copolymer containing a constitutional unit (A) based on 2-methacryloyloxyethyl phosphorylcholine and a constitutional unit (B) based on a photoreactive functional group-containing monomer, or a copolymer containing a constitutional unit (A) based on 2-methacryloyloxyethyl phosphorylcholine, a constitutional unit (B) based on a photoreactive functional group-containing monomer, and a constitutional unit (C) based on a hydrophobic group-containing monomer can impart durable lubricity to a substrate surface through a simple approach called photoirradiation.

The invention discloses a composite membrane for promoting wound repair by simulating a skin texture and a preparation method thereof. The preparation method comprises the following steps that: dissolving collagen (Col), chitosan (CS) and 2-methacryloyloxyethyl phosphorylcholine (MPC) at a mass ratio of (45-65):(20-30):(15-25) in a hexafluoroisopropanol solvent, and stirring into a homogeneous phase solution; pouring the homogeneous phase solution into a polytetrafluoroethylene mould, demoulding after volatilizing the solvent so as to obtain a compact composite membrane, and drying in a vacuum drying oven to completely volatilize the solvent; placing the dried composite membrane into a dryer to crosslink by glutaraldehyde vapor; cleaning the cross-linked composite membrane in water until removing glutaraldehyde in the composite membrane, and then vacuum drying the composite membrane to remove moisture; punching a plurality of through holes on the vacuum dried composite membrane by utilizing laser drilling, wherein the diameter of the through holes is 0.1-0.2 mm, and the space between the through holes is 0.8-1.2 mm, thereby obtaining the composite membrane for promoting the wound repair by simulating the skin texture.

The invention discloses a preparation method of an amphoteric polycarboxylate-type slump loss resistance agent. With 2-methacryloyloxyethyl phosphorylcholine, unsaturated long-chain polyether, unsaturated carboxylic acid and hydroxyl acrylate as monomer raw materials, under the function of an initiator and a chain transfer agent, the amphoteric polycarboxylate-type slump loss resistance agent is prepared by adopting an aqueous polymerization process. Compared with conventional polycarboxylate-type slump loss resistance agents, the prepared amphoteric polycarboxylate-type slump loss resistanceagent has longer slump loss resistance release time and better slump loss resistance performance, and can well meet the requirements of newly-mixed concrete for long-time and long-distance transportation and construction.