6 results about "Human melanoma" patented technology

The present invention relates to a polysaccharide with cytotoxic activity against human colon, human lung, human melanoma and murine leukaemia tumour cells, as well as antioxidant and healing activity. The invention also relates to a method for producing the polysaccharide, its uses and applications, particularly via drugs for treating cancer and / or antioxidant and healing compositions. The polysaccharide of the present invention can be produced from biomass, particularly from fungi, and preferably from the fungus Bovistella utriformis.

The present application relates to the field of animal model construction, and in particular to a melanoma liver metastasis model construction method, which uses a spleen injection method to construct a human melanoma A375-M1 cell line liver metastasis model, and is identified in vivo by PET-CT and MRI, and is identified ex vivo by Bouin's and H&E.The human melanoma liver metastasis tumor model constructed by the present application has the advantages of short construction time, simple method and 100% modeling success rate.This metastasis model simulates the development process and pathological characteristics of melanoma liver metastasis, solves the problem of the lack of an ideal animal model for human melanoma during liver metastasis, and helps to explore and screen the development of anti-liver metastasis drugs for human melanoma, and plays a positive promoting role in new strategies for anti-melanoma liver metastasis.

The invention relates to the field of fluorescent probes and biological detection, and discloses a tyrosinase and hydrogen sulfide double-target fluorescent probe as well as a preparation method and application thereof. The fluorescent probe provided by the invention is characterized in that the fluorescence of the fluorescent probe is extremely weak, but when tyrosinase and hydrogen sulfide coexist, a specific chemical reaction can occur, the fluorescence effect is obviously started, and strong green fluorescence is generated at 517nm, so that the detection on the activity of tyrosinase and the content of hydrogen sulfide is realized. The probe not only can be used for detecting content changes of tyrosinase and hydrogen sulfide in human primary melanocytes, but also can be used for imaging analysis of human melanoma tissue slices, and a new tool is provided for early diagnosis and histological research of melanoma.

The invention relates to the field of cell engineering, in particular to a melanoma verofenib drug-resistant cell strain as well as a construction method and application thereof. According to the invention, a parent human melanoma cell line A375 is used as a parent cell strain, verofenib is adopted to simulate an in-vivo treatment process, continuous treatment is carried out for 3 months, the drug resistance index of the cell strain to verofenib is detected through CCK-8, the cell strain resistant to verofenib is successfully screened out, the cell strain is named as the drug-resistant melanoma cell strain A375R homo sapiens, and the cell strain has the advantages that the drug resistance index of the drug-resistant melanoma cell strain A375R homo sapiens is shown in the description; the compound can be used for exploring a molecular mechanism or a signal channel for promoting or mediating the drug resistance of melanoma cells to the virofenib, and is used for exploring a molecular target spot for regulating and controlling the drug resistance of the melanoma virofenib, so that a targeted drug for reversing the drug resistance of the melanoma virofenib is developed. And the method is used for screening a drug resistance reversal agent of melanoma and searching a medication scheme for synergistically inhibiting drug resistance of a melanoma cell strain.

The invention discloses a polymer composition and application thereof in preparation of a medicine taking a Braf protein degradation agent as an active component. The polymer composition is prepared from the following raw materials: fucoidan, hydroxyethyl cellulose and polyvinyl alcohol, according to the high-molecular composition, fucoidan, hydroxyethyl cellulose and polyvinyl alcohol with different molecular weights are adopted, a cross-linked network structure is formed through the strong hydrogen-bond interaction among molecules, the good slow-release effect on the Braf protein degradation agent is achieved, and the stability of the Braf protein degradation agent can be effectively improved; in addition, the invention also provides a pharmaceutical composition, and the pharmaceutical composition has good inhibitory activity on SK-MEL-28 human melanoma cells, has a good degradation effect on Braf protein in the SK-MEL-28 human melanoma cells, and has a good potential treatment effect on solid tumors such as melanoma.

This invention relates to the field of animal model construction, specifically a method for constructing a lung metastasis model of the human melanoma A375-M1 cell line. The human melanoma A375-M1 cell line lung metastasis model was constructed using tail vein injection, and in vivo identification was performed using PET-CT and MRI, while in vitro identification was performed using Bouin's and H&E assays. The human melanoma lung metastasis model constructed by this invention has the advantages of short construction time, simple method, and 100% modeling success rate. This metastasis model simulates the development process and pathological characteristics of melanoma lung metastasis, solving the problem of the lack of ideal animal models for human melanoma lung metastasis. It contributes to the exploration and screening of anti-lung metastasis drugs for human melanoma and plays a positive role in promoting new strategies for combating melanoma lung metastasis.