11 results about "Human melanoma" patented technology

The present invention relates to a polysaccharide with cytotoxic activity against human colon, human lung, human melanoma and murine leukaemia tumour cells, as well as antioxidant and healing activity. The invention also relates to a method for producing the polysaccharide, its uses and applications, particularly via drugs for treating cancer and / or antioxidant and healing compositions. The polysaccharide of the present invention can be produced from biomass, particularly from fungi, and preferably from the fungus Bovistella utriformis.

The present application relates to the field of animal model construction, and in particular to a melanoma liver metastasis model construction method, which uses a spleen injection method to construct a human melanoma A375-M1 cell line liver metastasis model, and is identified in vivo by PET-CT and MRI, and is identified ex vivo by Bouin's and H&E.The human melanoma liver metastasis tumor model constructed by the present application has the advantages of short construction time, simple method and 100% modeling success rate.This metastasis model simulates the development process and pathological characteristics of melanoma liver metastasis, solves the problem of the lack of an ideal animal model for human melanoma during liver metastasis, and helps to explore and screen the development of anti-liver metastasis drugs for human melanoma, and plays a positive promoting role in new strategies for anti-melanoma liver metastasis.

The invention relates to the field of fluorescent probes and biological detection, and discloses a tyrosinase and hydrogen sulfide double-target fluorescent probe as well as a preparation method and application thereof. The fluorescent probe provided by the invention is characterized in that the fluorescence of the fluorescent probe is extremely weak, but when tyrosinase and hydrogen sulfide coexist, a specific chemical reaction can occur, the fluorescence effect is obviously started, and strong green fluorescence is generated at 517nm, so that the detection on the activity of tyrosinase and the content of hydrogen sulfide is realized. The probe not only can be used for detecting content changes of tyrosinase and hydrogen sulfide in human primary melanocytes, but also can be used for imaging analysis of human melanoma tissue slices, and a new tool is provided for early diagnosis and histological research of melanoma.

The invention discloses an application of a herba ecliptae extract in preparation of a whitening product. The skin-whitening product takes the herba perillae extract as a main functional component, and comprises a skin-care product, a washing care product and an aromatherapy product with a skin-whitening effect. The herba ecliptae extract comprises guaiacol, alpha-eudesmol, beta-eudesmol, D-camphor and beta-caryophyllene, and is obtained by performing steam distillation extraction on the overground part of herba ecliptae as a raw material. The invention discloses an effect of inhibiting melanin synthesis of the herba perillae acutae extract, can directly reduce the melanin content of human melanoma cells MNT1, and also can indirectly reduce the melanin content of the MNT1 cells by influencing human immortalized keratinocytes HaCaT. Under the same mass concentration, the effect of the whitening agent is superior to that of the whitening agents alpha-arbutin and kojic acid which are widely used at present. Therefore, the herba perillae extract can be used as a natural extract component to be applied to development of related whitening products such as cosmetic technology and aromatherapy.

The invention discloses a radix et rhizoma rhei extract as well as a preparation method and application of the radix et rhizoma rhei extract. The radix et rhizoma rhei extract is prepared from eudesmol, 4-isopropyl toluene, beta-pinene, gamma-terpinene and Helminthogermacene. The preparation method of the rheum officinale extract comprises the step of extracting the overground part of rheum officinale through a steam distillation method. The invention also discloses an application of the rheum officinale extract in preparation of a product with a whitening effect. The Rheum officinale extract has oxidation resistance and melanin synthesis inhibition capability, can directly reduce the melanin content of human melanoma cells MNT1, can indirectly reduce the melanin content of the MNT1 cells by influencing human immortalized keratinocytes HaCaT, and has better effects than the whitening agents alpha-arbutin and kojic acid which are widely used at present. Therefore, the rheum officinale extract can be used as a natural extract component to be applied to the fields of cosmetic technology and aromatherapy and the development of whitening products in related industries.

The invention relates to the field of cell engineering, in particular to a melanoma verofenib drug-resistant cell strain as well as a construction method and application thereof. According to the invention, a parent human melanoma cell line A375 is used as a parent cell strain, verofenib is adopted to simulate an in-vivo treatment process, continuous treatment is carried out for 3 months, the drug resistance index of the cell strain to verofenib is detected through CCK-8, the cell strain resistant to verofenib is successfully screened out, the cell strain is named as the drug-resistant melanoma cell strain A375R homo sapiens, and the cell strain has the advantages that the drug resistance index of the drug-resistant melanoma cell strain A375R homo sapiens is shown in the description; the compound can be used for exploring a molecular mechanism or a signal channel for promoting or mediating the drug resistance of melanoma cells to the virofenib, and is used for exploring a molecular target spot for regulating and controlling the drug resistance of the melanoma virofenib, so that a targeted drug for reversing the drug resistance of the melanoma virofenib is developed. And the method is used for screening a drug resistance reversal agent of melanoma and searching a medication scheme for synergistically inhibiting drug resistance of a melanoma cell strain.

The invention discloses a polymer composition and application thereof in preparation of a medicine taking a Braf protein degradation agent as an active component. The polymer composition is prepared from the following raw materials: fucoidan, hydroxyethyl cellulose and polyvinyl alcohol, according to the high-molecular composition, fucoidan, hydroxyethyl cellulose and polyvinyl alcohol with different molecular weights are adopted, a cross-linked network structure is formed through the strong hydrogen-bond interaction among molecules, the good slow-release effect on the Braf protein degradation agent is achieved, and the stability of the Braf protein degradation agent can be effectively improved; in addition, the invention also provides a pharmaceutical composition, and the pharmaceutical composition has good inhibitory activity on SK-MEL-28 human melanoma cells, has a good degradation effect on Braf protein in the SK-MEL-28 human melanoma cells, and has a good potential treatment effect on solid tumors such as melanoma.

This invention relates to the field of animal model construction, specifically a method for constructing a lung metastasis model of the human melanoma A375-M1 cell line. The human melanoma A375-M1 cell line lung metastasis model was constructed using tail vein injection, and in vivo identification was performed using PET-CT and MRI, while in vitro identification was performed using Bouin's and H&E assays. The human melanoma lung metastasis model constructed by this invention has the advantages of short construction time, simple method, and 100% modeling success rate. This metastasis model simulates the development process and pathological characteristics of melanoma lung metastasis, solving the problem of the lack of ideal animal models for human melanoma lung metastasis. It contributes to the exploration and screening of anti-lung metastasis drugs for human melanoma and plays a positive role in promoting new strategies for combating melanoma lung metastasis.

The application discloses a use of a DDX51 gene in human melanoma and related products. The application takes the DDX51 gene as a template, designs an RNA interference target sequence, and constructs a target gene RNA interference lentivirus vector. RT-PCR detection of the expression of the target gene in target cells, lentivirus infection of the target cells, qPCR detection of the mRNA level DDX51 gene knockdown efficiency, Celigo detection of the influence of the DDX51 gene knockdown on cell proliferation, detection of the influence of the DDX51 gene knockdown on clone formation, Invasion (Transwell with ECM) detection of the influence of the DDX51 gene knockdown on the cell invasion ability, and the like confirm that the interference target gene DDX51 has the effects of inhibiting the proliferation, clone formation, invasion and metastasis of human melanoma cells A375.

The present invention discloses an anti-tumor compound, a preparation method, a pharmaceutical composition containing the compound and an application, belonging to the field of biomedical technology. The compound of the present invention has remarkable effects in in vitro anti-tumor activity tests. During the design process of the present invention, N 3 -((4,6-dimethyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-N 3 '-((R)-[1,1'-biphenyl]-3,3'-dicarboxamide compounds are designed and synthesized, which improve the anti-tumor effect, and it is found that they have good anti-proliferation ability against human melanoma cell line A375. During the synthesis process, the synthesis steps are optimized, providing a possibility for future industrial production.

The invention discloses (4-oxopentyl-1-alkynyl) (triphenylphosphine) gold (I) and application thereof in preparation of a medicine for treating melanoma, and belongs to the field of biological medicine. The invention provides novel (4-oxopentyl-1-alkynyl) (triphenylphosphine) gold (I) which can effectively resist melanoma by inhibiting proliferation and growth of melanoma cells, has low side effects, can be used as a candidate drug for treating melanoma-related diseases caused by various reasons, and can be used as a novel drug for treating melanoma-related diseases caused by various reasons. The application prospect of creating the medicine for treating the melanoma is good, a new medicine alternative is provided for treating the human melanoma, and the problems that the current treatment medicine is relatively single and has medicine resistance can be relieved.