38 results about "Juglone" patented technology

The juglone extracting process includes the following steps: 1. crushing the root, stem, leaf, bark or pericarp of Juglans mandshurica as the material to below 20 mesh; 2. soaking the material in solvent of 1 to 1000 times weight through stirring for 0-12 hr; 3. distilling the soaked material under reduced pressure while replenishing solvent to obtain colorless distillate; 4. separating out the product physically from the distillate and drying to obtain juglone-containing product. The present invention has simple process, short production period, low cost, high product purity and yield, and other advantages.

The invention discloses a method for extracting juglone from walnut green husk residue by microwave reflux, which ueses chloroform solvent for extraction by a microwave reflux extraction method, preferably adopting walnut epicarpe (green husk) residue with higher juglone content and can be obtained easily as raw materials for extraction. The method is carried out under the optimal extraction condition that the solid-to-liquid ratio of walnut green husk with average grain size of 50 mesh to the chloroform solvent is between 1:5 and 1:8, and comprises the steps of: subjecting walnut green husk residue to extraction and pumping filtration at soft fire in microwave power level and washing the filter residue with solvent to obtain juglone extraction liquid, collecting all solutions, and preparing the juglone by decompression, rotary evaporation and concentration. After all index measurements, the extraction ratio of the juglone is 92%, the purity of product is 81.5%, and the product yield is 0.20%. The method is rapid and convenient, and has high extraction ratio, and devices in the method are easy to operate, have low energy consumption, consume less solvents which are easy to be recovered, and are suitable for the expanded production in factories. Meanwhile, the problem that juglone is heated and decomposed in a traditional method can be effectively avoided, which guarantees the effectiveness of extractives. The method satisfies the environmental protection and industrialization demands. The extracted juglone can be widely applied, and active ingredients in the juglone have the effects of antibacterial, anticancer and sedation of central nervous system, and have wide application prospects in such fields as medicine, pesticide, dye, pH indicator and the like.

The invention discloses a preparation method of pigment constituents of cortex juglandis mandshuricae, namely polyphenol and juglone parts of cortex juglandis mandshuricae, and also provides different-colored hair dyes of cortex juglandis mandshuricae and a hair dyeing method thereof. Each different-colored hair dye comprises an agent A, an agent B and an agent C which are used along with each other, wherein the agent A comprises, by weight, 0.5-1% of vitamin E, 1-5% of a metal salt mordant, 1-10% of a surfactant, 1-5% of a thickening agent and the balance being deionized water; the agent B comprises, by weight, 5-30% of one or two of the part I and the part II of cortex juglandis mandshuricae, 1-10% of a plant penetrant, 3-9% of hydrogen peroxide, 1-5% of a thickening agent and the balance being deionized water; and the agent C comprises, by weight, 5-30% of one or two of the part I and the part II of cortex juglandis mandshuricae, 1-10% of a plant penetrant, 1-5% of a thickening agent and the balance being deionized water. The invention has advantages as follows: the preparation is convenient and efficient; raw materials are easily available; and the constituents of cortex juglandis mandshuricae can be repeatedly utilized. The different-colored hair dyes belong to natural plant extracts, are safe and reliable, have various color systems and are suitable for colored dyeing of black hair, white hair and grizzled hair.

The present invention relates to application of juglans plant extract as pesticide in preventing and controlling diamondback moth. The leaf, fruit coating and branch bark of juglans plant are used as main material to prepare juglone liquid, and the juglone liquid is then compounded into pesticide alone or together with other farm pesticide for preventing and controlling diamondback moth. The present invention has high pesticidal activity on diamondback moth, and compared with other chemical pesticide, the botanical pesticide is more environmental friendly.

The invention relates to a method for extracting polyphenol substances from semen juglandis with inner seed coats by the aid of combined enzyme and ultrasonic treatment. The method includes acquiring the semen juglandis with the seed coats; feeding the semen juglandis with the inner seed coats into ethanol aqueous solution according to a volume ratio of 1:3-1:15 to obtain mixtures, regulating the pH (potential of hydrogen) of the mixtures by the aid of food-grade citric acid or hydrochloric acid until the pH of the mixtures is 3.5-6.5 and then adding pectinase and / or cellulase compounds into the mixtures to obtain polyphenol extracted systems. The method has the advantages that the total weight of dry substances, which are obtained after freeze drying is carried out on semen juglandis polyphenol crude extract prepared by the aid of the method, at least accounts for 2% of the weight of the semen juglandis with the coats, and the contents of polyphenol effective substances in the semen juglandis polyphenol crude extract can reach 15% at least; the semen juglandis polyphenol crude extract further can be separated by the aid of macroporous adsorption resin and industrial chromatographs, and accordingly products such as ellagic acid, hydrolysable tannin and juglone can be prepared.

The invention discloses a method for supercritical fluid extraction of juglone in walnut seed green skin waste residue. In the invention, supercritical CO2 fluid in the supercritical fluid extraction method is adopted for extracting, walnut seed external skin (green skin) waste residue which is high in juglone content and easily available is preferably selected as the raw material for extraction. The best conditions are as follows: flow rate of the supercritical CO2 fluid is 1-3L / min, the extraction pressure is 10-18MPa, the extraction temperature is below 65 DEG C and the extraction time is within 20min. In the invention, one-time extraction yield of the juglone reaches 87%, the product purity is 92%, the productive rate is 0.21%. The method of the invention features not only high extraction yield but also innocuity, no taste, incombustibility, no erosion, low price and easy recovering of the extraction liquid, thus meeting the requirements of environmental protection and industrialization. The extracted juglone is wide in application, the active ingredients play a role in resisting bacterium and cancer and calming nervus centralis, thus enjoying very wide application prospect in medicine, agricultural chemical, dye and pH indicator and other fields.

The invention relates to an extraction method of chemical components in walnut green tangerine peel, particularly an extraction method of juglone and derivative in the walnut green tangerine peel. The extraction method comprises the following steps: pealing fresh walnut green tangerine peel, and sieving by a screen mesh of 40-80 meshes after washing, drying and crushing; putting the sieved walnut green tangerine peel powder into a container, and adding pure water according to a solid-liquid quality ratio of 1: (8-12); adding pectase and cellulase in the container, sealing the container after regulating the ph values of the solution in the container to 5.5-6.7, and then oscillating the resultant at the constant temperature of 20-40 DEG C for 36-60h; reducing pressure of and condensing the solution after enzymatic hydrolysis to obtain a concentrate; and extracting the resultant by petroleum ether after freezing and drying the concentrate to obtain juglone and derivative. In the invention, walnut green tangerine peel cell wall is destroyed by enzymatic hydrolysis, and recovery rate is raised by microwave ultrasonic wave processing; the technology of obtaining juglone and derivative by petroleum ether extraction is simple with high recovery rate, and the problems of low pericarp salvaging and low quinone substance recovery rate in walnut plucking and processing are solved.

The germicide preparation for preventing and controlling the damping-off of rhodiola is compounded with juglone, including 5-hydroxy-1,4-naphthoquinone and 5,8-dihydroxy-1,4-naphthoquinone, as the effective component in green fruit peel and leaf of Juglans mandshurica. It exhibits excellent effect of inhibiting the growth of ptotomiliary body of damping-off pathogen and is effective preparation for preventing and controlling the damping-off of rhodiola.

The invention discloses a medicament for inhibiting root growth of stored garlic. The medicament is a water solution containing juglone, wherein the concentration of juglone is 0.001-1%; juglone is wide in raw material source, can be used as an extract of a natural juglone plant and also juglone synthesized chemically, and is easy to prepare and low in cost. The medicament is convenient to use; the garlic is only soaked with the medicament for a while and then aired; the medicament is low in juglone dosage, non-toxic, environment-friendly, and capable of effectively inhibiting root growth of the garlic and effectively preventing garlic rot, and cannot inhibit seedling growth of the garlic.

A Chinese medicine in the form of softgel for improving thinking and memory power, preventing and relaxing arteriosclerosis, hypertension, heart disease, cerebral apoplexy, and senile dementia, and delaying the sanility of cerebral cells is proportionally prepared from the powdered extract of jujube and polygala root, soybean lecithin, walnut oil and beeswax. Its preparing process is also disclosed.

The invention discloses application of juglone to termite control, i.e., a juglone-containing agent is sprayed to places where termites haunt about to fulfill the aim of controlling the termites. The juglone has the advantages of wide source, readily available raw material, simple structure, easiness in synthesis and obvious control effect on the termites. The juglone has a synergistic effect after being mixed with other termite control agents for use; and the growth of a target plant can also be promoted by a juglone-containing plant extract. The juglone is originated from plants, has a smooth degradation approach in nature, and has the advantages of low toxicity to human and animals, no environmental pollution, no residue, no influence on water resource, capability of meeting the environmental protection requirement of the termite control agent and broad application prospect compared with other termite control agents.

The invention discloses the application of juglone nucin to resistance to eimeria tenella. The juglone nucin is uniformly mixed with acceptable auxiliary materials and the mixture is added into water or feeds to effectively resist the eimeria tenella.

Multifunctional compositions and methods are provided for therapeutic treatment of bacteria and cancers and for fluorescence diagnosis. Systems generate in situ reactive oxygen species such as singlet oxygen (1O2), hydroxyl radical (OH) and Juglone, and other chemotherapeutic agents. Methods provided selectively produce greater amounts of one reactive oxygen species over others. Variations are effective in aerobic, anaerobic or H2O2 rich environments and in presence of, or absence of, light. In H2O2 rich environment in absence of light, variations decompose H2O2 into O2 gas to remove excess H2O2 for elimination of hypoxic environment. Variations are formed of porphyrins, naphthalene derivatives, and metal ions, for illustration, free base tetrakis Ar substituted porphyrine core without metal or halide substitution but having hydroxyphenyl and alkyl pyridyl substituents at meso positions combined with dihydroxynaphthalene and +3 hydrated metal ions.

The invention discloses a biopesticide for killing oncomelania, which comprises the following materials in parts by weight: 35-64 parts of nicotine, 11-19 parts of celastrus angulatus, 10-16 parts of paraquat, 3-7 parts of sapium sebiferum, 15-20 parts of kushenin, 11-14 parts of santonin water aqua, 3-6 parts of fructus amomi, 10-12 parts of juglone, 25-36 parts of broad beans, 45-50 parts of ethanol, 5-11 parts of hirsutella thompsonii fisher powder and 35-40 parts of sanguinarine. The biopesticide disclosed by the invention has a good killing effect, and is quite obvious in function of killing oncomelania under a low concentration; and the biopesticide is low in toxicity to human, animals, aquatic plants and other aquatic animals, and is capable of relieving pollution to soil and water quality. The biopesticide disclosed by the invention is simple in formula, and easy and simple in preparation method.

The invention belongs to the field of traditional Chinese medicine preparations, relates to a novel use of juglone having a structure shown in a formula (I) in pharmacy, and particularly relates to a use of the juglone in preparation of medicines treating brain tumor. In-vitro U87 spongioblastoma cell line killing tests and animal tests on a spongioblastoma model are performed. Results of the tests show that: the IC50 of the juglone in the killing tests is 6 [mu]g / mL; in the animal tests, the lifetime of a juglone group is longer than that of a contrast group; and by systematic antitumor medicine evaluation, the results prove that the juglone has significant inhibiting functions for the spongioblastoma in vitro and in vivo, and can be used for preparation of chemotherapeutics for treating the brain tumor especially the spongioblastoma. The formula (I) is shown in the specification.

The invention discloses a method for supercritical fluid extraction of juglone in walnut seed green skin waste residue. In the invention, supercritical CO2 fluid in the supercritical fluid extraction method is adopted for extracting, walnut seed external skin (green skin) waste residue which is high in juglone content and easily available is preferably selected as the raw material for extraction. The best conditions are as follows: flow rate of the supercritical CO2 fluid is 1-3L / min, the extraction pressure is 10-18MPa, the extraction temperature is below 65 DEG C and the extraction time is within 20min. In the invention, one-time extraction yield of the juglone reaches 87%, the product purity is 92%, the productive rate is 0.21%. The method of the invention features not only high extraction yield but also innocuity, no taste, incombustibility, no erosion, low price and easy recovering of the extraction liquid, thus meeting the requirements of environmental protection and industrialization. The extracted juglone is wide in application, the active ingredients play a role in resisting bacterium and cancer and calming nervus centralis, thus enjoying very wide application prospect inmedicine, agricultural chemical, dye and pH indicator and other fields.

The invention provides a method for separating and purifying juglone in walnut shells, wherein the method for separating and purifying juglone in the walnut shells comprises the following steps: S1, cleaning and drying the walnut shells, crushing the walnut shells by a crusher, and sieving the crushed walnut shells; S2, taking 20 g of walnut shell powder, placing in a steam explosion instrument, and pretreating; and S3, adding the walnut shell powder pretreated in the S2 into a solvent, and performing ultrasonic extraction at normal temperature, completely dissolving organic substances in the walnut shell powder into the solvent to obtain a mixed extracting solution. According to the method, the walnut shells are firstly crushed into the walnut shell powder, then purified crystals are obtained through the methods of steam explosion pretreatment, ultrasonic extraction and column chromatography purification, the process route is simple and feasible, juglone can be effectively extracted, separated and purified from the walnut shells, the product yield can reach 0.2% or above, waste utilization is facilitated, and the problem of ecological environment pollution caused by waste of a large number of walnut shells is avoided.

The invention relates to the technical field of medicines, discovers an application of juglone for adjusting immune states of mice and oxidizing reactions, and mainly researches medicinal composition juglone. A chemical name of the juglone is 5-hydroxy-1,4-naphthoquinone and 5-hydroxy-1,4-naphthalene diketone. An English name of the juglone is Juglone, walnuts quinine, 5-hydroxy-1,4-naphthoquinone and 5-hydroxynaphthalene, regianin, Nucin. A molecular formula of the juglone is C10H6O3. A relative molecular weight is 174.15. An immunocompromised model is built through systematic pharmacological tests and by using KM mice and adopting dexamethasone sodium phosphate injecta, a mouse immunostimulation model is built by using sheep red blood cells (SRBC) to induce the mice to produce SRBC immune bodies and cyclophosphamide, a fact that the juglone can recover low immunologic functions to a normal level is proved, the immunostimulation states are improved, self stability of an organism is maintained, and both-way immunoregulation functions are displayed. The juglone displays functions of adjusting organism immune system functions by adjusting oxidative stress reactions in the organism.

The invention relates to the technical field of pesticides, in particular to a plant herbicide. The plant herbicide is prepared from the following materials in parts by weight: 3-12 parts of 9-carbonyl-10,11-dehydroageraphorone, 5-12 parts of juglone, 10-18 parts of a ragweed extract, 6-16 parts of a carpesium cernuum extract, 1-8 parts of azadirachtin, 1-4 parts of triacontanol, 0.5-3 parts of pyromellitic sodium and 1-4 parts of an emulsifying agent. The plant herbicide provided by the invention has the characteristics of rapid response, obvious weeding effect, no pesticide residue, and lowpossiblity of causing drug resistance and accumulation of toxic and side effects.

The invention discloses a hand and foot chapping nursing composition and a preparing method thereof .The hand and foot chapping nursing composition is mainly prepared from, by weight, 2-8 parts of sphingolipid, 1-5 parts of glycyrrhizin, 2-4 parts of asiaticoside, 3-6 parts of acetylsalicylic acid, 1-2 parts of theanine, 0.2-0.3 part of interleukin, 1-2 parts of oleanolic acid, 3-8 parts of sugar alcohol, 0.5-1.5 parts of beta-oxidized juglone, 3-6 parts of plant essential oil, 1-4 parts of water soluble vitamin and 3-6 parts of alginic acid .By combining the components in certain proportion, the functions of antisepsis and anti-inflammation, anti-infection, relieving swelling, pains and itching, removing necrosis, promoting granulation, promoting blood circulation, avoiding cicatrization and beautifying and nursing skin are realized, and then quick healing of chapped wounds is accelerated .The composite has a remarkable clinical treatment effect.

A process for easily and inexpensively producing in high yield 2-(1-hydroxyethyl)-5-hydroxynaphtho[2,3-b]furan-4,9-dione which is a racemic modification or an optically active substance; 2-acetyl-2,3-dihydro-5-hydroxynaphtho[2,3-b]furan-4,9-dione, which is useful as an intermediate for producing NFD; and an anticancer containing 2-(1-hydroxyethyl)-5-hydroxynaphtho[2,3-b]-furan-4,9-dione as an active ingredient. 5-Hydroxynaphthalene-1,4-dione (another name: Juglone), which is available at a relatively low cost, is used as a starting material to obtain 2-(1-hydroxyethyl)-5-hydroxynaphtho[2,3-b]furan-4,9-dione through four or five steps.

The invention discloses an application of juglone to preparation of a medicament for treating autoimmune and inflammatory diseases. As proved by an experimental result, the juglone can be used for lowering the disease incidence, average clinical score, highest clinical score and total clinical scores of mouse experimental autoimmune encephalomyelitis, lowering the percentages of an IFN (Interferon)-gamma secretion-relevant cell Th1 and a 1L-17A secretion-relevant cell Th17 in spinal marrow of mouse experimental autoimmune encephalomyelitis in a CD4+T cell, inhibiting the proliferation of a cell T induced by MOG (Myelin Oligodendrocyte Glycoprotein) of the mouse experimental autoimmune encephalomyelitis, and lowering the expression of CD11b+ angtigen presentation cells CD83 and MHCII in the spleen of the mouse experimental autoimmune encephalomyelitis. The juglone for preparing a medicament for inhibiting an immune system related to the invention is expected to become a medicament for clinically treating autoimmune inflammatory diseases such as multiple sclerosis, acute disseminated encephalomyelitis and the like.

The invention provides an application of forsythia flower yellow pigment in preparing an anti-oxidative stress injury drug. The preparation step of the yellow pigment is as follows: adding dried forsythia flower powder into 95% ethanol according to a solid-liquid ratio of 1:100, performing water bath at 50 DEG C, and performing extraction by a hot reflux method; and performing suction filtration separation, repeating extraction twice, collecting the extractive liquid, performing rotary evaporation concentration, carrying out freeze drying, and collecting dry powder. The yellow pigment contains: rutin, forsythoside A, rosin, forsythin and the like. The yellow pigment can effectively eliminate ROS free radicals in caenorhabditis elegans body by up-regulating the expression of DAF-16 and SKN-1 target genes, increase the activity of antioxidant enzymes in elegans body, reduce the free radical content in the elegans body and weaken the oxidative stress damage in the elegans body by juglone,and can significantly prolong the lifespan of wild-type elegans N2 and daf-16 mutant elegans under oxidative stress, thereby increasing the resistance of elegans to oxidative stress. The yellow pigment can also be used in the preparation of functional foods resistant to oxidative stress damage.

The present invention relates to application of juglans plant extract as pesticide in preventing and controlling diamondback moth. The leaf, fruit coating and branch bark of juglans plant are used as main material to prepare juglone liquid, and the juglone liquid is then compounded into pesticide alone or together with other farm pesticide for preventing and controlling diamondback moth. The present invention has high pesticidal activity on diamondback moth, and compared with other chemical pesticide, the botanical pesticide is more environmental friendly.

A method for extracting polyphenol substances from semen juglandis with inner seed coats by the aid of combined enzyme and ultrasonic treatment. The method comprises: obtaining semen juglandis with seed coats; feeding the semen juglandis with inner seed coats into an ethanol aqueous solution according to a volume ratio of 1:3-1:15, regulating the pH of the mixture by the aid of food-grade citric acid or hydrochloric acid until the pH of the mixture is 3.5-6.5, and then adding pectinase and / or a cellulase compound to obtain a polyphenol extracted system. According to the semen juglandis polyphenol crude extract prepared by the method, the total weight of dry substances, which are obtained after freeze drying, at least accounts for 2% of the weight of the original semen juglandis with the coats, and the contents of polyphenol effective substances in the semen juglandis polyphenol crude extract can reach 15% at least; the semen juglandis polyphenol crude extract further can be separated by the aid of macroporous adsorption resin and industrial chromatographs, and accordingly products such as ellagic acid, hydrolysable tannin, and juglone can be prepared.

The invention discloses the application of juglone nucin to resistance to eimeria tenella. The juglone nucin is uniformly mixed with acceptable auxiliary materials and the mixture is added into water or feeds to effectively resist the eimeria tenella.

Multifunctional compositions and methods are provided for therapeutic treatment of bacteria and cancers and for fluorescence diagnosis. Systems generate in situ reactive oxygen species such as singlet oxygen (1O2), hydroxyl radical (OH) and Juglone, and other chemotherapeutic agents. Methods provided selectively produce greater amounts of one reactive oxygen species over others. Variations are effective in aerobic, anaerobic or H2O2 rich environments and in presence of, or absence of, light. In H2O2 rich environment in absence of light, variations decompose H2O2 into O2 gas to remove excess H2O2 for elimination of hypoxic environment. Variations are formed of porphyrins, naphthalene derivatives, and metal ions, for illustration, free base tetrakis Ar substituted porphyrine core without metal or halide substitution but having hydroxyphenyl and alkyl pyridyl substituents at meso positions combined with dihydroxynaphthalene and +3 hydrated metal ions.

A method for extracting juglone, relating to an extraction method. The present invention solves the problems of complex extraction and separation methods, low extract purity and low extract yield in the prior art, and the extraction steps are as follows: a, the raw material is the root, stem, leaf, skin or pericarp of Juglans catalpa Pulverize to below 20 mesh; b, weigh the raw material, add water according to the weight ratio of raw material and water in the ratio of 1: 1 to 1: 1000, stir, and soak for 0 to 12 hours; c, carry out vacuum distillation on the soaked material, and add Add water until the distillate is colorless; d. Collect the liquid distilled in step c, separate the product by physical methods, and then dry the product; that is, a product containing juglone is obtained. The invention has the advantages of low equipment requirement, easy implementation, simple steps, short production cycle, flexible arrangement of production schedule, low production cost, economic reliability and high extract purity and yield.

The invention relates to an extraction method of chemical components in walnut green tangerine peel, particularly an extraction method of juglone and derivative in the walnut green tangerine peel. The extraction method comprises the following steps: pealing fresh walnut green tangerine peel, and sieving by a screen mesh of 40-80 meshes after washing, drying and crushing; putting the sieved walnut green tangerine peel powder into a container, and adding pure water according to a solid-liquid quality ratio of 1: (8-12); adding pectase and cellulase in the container, sealing the container after regulating the ph values of the solution in the container to 5.5-6.7, and then oscillating the resultant at the constant temperature of 20-40 DEG C for 36-60h; reducing pressure of and condensing the solution after enzymatic hydrolysis to obtain a concentrate; and extracting the resultant by petroleum ether after freezing and drying the concentrate to obtain juglone and derivative. In the invention, walnut green tangerine peel cell wall is destroyed by enzymatic hydrolysis, and recovery rate is raised by microwave ultrasonic wave processing; the technology of obtaining juglone and derivative by petroleum ether extraction is simple with high recovery rate, and the problems of low pericarp salvaging and low quinone substance recovery rate in walnut plucking and processing are solved.