38 results about "Juglone" patented technology

The juglone extracting process includes the following steps: 1. crushing the root, stem, leaf, bark or pericarp of Juglans mandshurica as the material to below 20 mesh; 2. soaking the material in solvent of 1 to 1000 times weight through stirring for 0-12 hr; 3. distilling the soaked material under reduced pressure while replenishing solvent to obtain colorless distillate; 4. separating out the product physically from the distillate and drying to obtain juglone-containing product. The present invention has simple process, short production period, low cost, high product purity and yield, and other advantages.

The invention discloses a method for extracting juglone from walnut green husk residue by microwave reflux, which ueses chloroform solvent for extraction by a microwave reflux extraction method, preferably adopting walnut epicarpe (green husk) residue with higher juglone content and can be obtained easily as raw materials for extraction. The method is carried out under the optimal extraction condition that the solid-to-liquid ratio of walnut green husk with average grain size of 50 mesh to the chloroform solvent is between 1:5 and 1:8, and comprises the steps of: subjecting walnut green husk residue to extraction and pumping filtration at soft fire in microwave power level and washing the filter residue with solvent to obtain juglone extraction liquid, collecting all solutions, and preparing the juglone by decompression, rotary evaporation and concentration. After all index measurements, the extraction ratio of the juglone is 92%, the purity of product is 81.5%, and the product yield is 0.20%. The method is rapid and convenient, and has high extraction ratio, and devices in the method are easy to operate, have low energy consumption, consume less solvents which are easy to be recovered, and are suitable for the expanded production in factories. Meanwhile, the problem that juglone is heated and decomposed in a traditional method can be effectively avoided, which guarantees the effectiveness of extractives. The method satisfies the environmental protection and industrialization demands. The extracted juglone can be widely applied, and active ingredients in the juglone have the effects of antibacterial, anticancer and sedation of central nervous system, and have wide application prospects in such fields as medicine, pesticide, dye, pH indicator and the like.

The present invention relates to application of juglans plant extract as pesticide in preventing and controlling diamondback moth. The leaf, fruit coating and branch bark of juglans plant are used as main material to prepare juglone liquid, and the juglone liquid is then compounded into pesticide alone or together with other farm pesticide for preventing and controlling diamondback moth. The present invention has high pesticidal activity on diamondback moth, and compared with other chemical pesticide, the botanical pesticide is more environmental friendly.

The invention discloses a method for supercritical fluid extraction of juglone in walnut seed green skin waste residue. In the invention, supercritical CO2 fluid in the supercritical fluid extraction method is adopted for extracting, walnut seed external skin (green skin) waste residue which is high in juglone content and easily available is preferably selected as the raw material for extraction. The best conditions are as follows: flow rate of the supercritical CO2 fluid is 1-3L/min, the extraction pressure is 10-18MPa, the extraction temperature is below 65 DEG C and the extraction time is within 20min. In the invention, one-time extraction yield of the juglone reaches 87%, the product purity is 92%, the productive rate is 0.21%. The method of the invention features not only high extraction yield but also innocuity, no taste, incombustibility, no erosion, low price and easy recovering of the extraction liquid, thus meeting the requirements of environmental protection and industrialization. The extracted juglone is wide in application, the active ingredients play a role in resisting bacterium and cancer and calming nervus centralis, thus enjoying very wide application prospect in medicine, agricultural chemical, dye and pH indicator and other fields.

The invention relates to an extraction method of chemical components in walnut green tangerine peel, particularly an extraction method of juglone and derivative in the walnut green tangerine peel. The extraction method comprises the following steps: pealing fresh walnut green tangerine peel, and sieving by a screen mesh of 40-80 meshes after washing, drying and crushing; putting the sieved walnut green tangerine peel powder into a container, and adding pure water according to a solid-liquid quality ratio of 1: (8-12); adding pectase and cellulase in the container, sealing the container after regulating the ph values of the solution in the container to 5.5-6.7, and then oscillating the resultant at the constant temperature of 20-40 DEG C for 36-60h; reducing pressure of and condensing the solution after enzymatic hydrolysis to obtain a concentrate; and extracting the resultant by petroleum ether after freezing and drying the concentrate to obtain juglone and derivative. In the invention, walnut green tangerine peel cell wall is destroyed by enzymatic hydrolysis, and recovery rate is raised by microwave ultrasonic wave processing; the technology of obtaining juglone and derivative by petroleum ether extraction is simple with high recovery rate, and the problems of low pericarp salvaging and low quinone substance recovery rate in walnut plucking and processing are solved.

The germicide preparation for preventing and controlling the damping-off of rhodiola is compounded with juglone, including 5-hydroxy-1,4-naphthoquinone and 5,8-dihydroxy-1,4-naphthoquinone, as the effective component in green fruit peel and leaf of Juglans mandshurica. It exhibits excellent effect of inhibiting the growth of ptotomiliary body of damping-off pathogen and is effective preparation for preventing and controlling the damping-off of rhodiola.

A Chinese medicine in the form of softgel for improving thinking and memory power, preventing and relaxing arteriosclerosis, hypertension, heart disease, cerebral apoplexy, and senile dementia, and delaying the sanility of cerebral cells is proportionally prepared from the powdered extract of jujube and polygala root, soybean lecithin, walnut oil and beeswax. Its preparing process is also disclosed.

The invention discloses application of juglone to termite control, i.e., a juglone-containing agent is sprayed to places where termites haunt about to fulfill the aim of controlling the termites. The juglone has the advantages of wide source, readily available raw material, simple structure, easiness in synthesis and obvious control effect on the termites. The juglone has a synergistic effect after being mixed with other termite control agents for use; and the growth of a target plant can also be promoted by a juglone-containing plant extract. The juglone is originated from plants, has a smooth degradation approach in nature, and has the advantages of low toxicity to human and animals, no environmental pollution, no residue, no influence on water resource, capability of meeting the environmental protection requirement of the termite control agent and broad application prospect compared with other termite control agents.

The invention discloses the application of juglone nucin to resistance to eimeria tenella. The juglone nucin is uniformly mixed with acceptable auxiliary materials and the mixture is added into water or feeds to effectively resist the eimeria tenella.

Multifunctional compositions and methods are provided for therapeutic treatment of bacteria and cancers and for fluorescence diagnosis. Systems generate in situ reactive oxygen species such as singlet oxygen (¹O₂), hydroxyl radical (OH) and Juglone, and other chemotherapeutic agents. Methods provided selectively produce greater amounts of one reactive oxygen species over others. Variations are effective in aerobic, anaerobic or H₂O₂ rich environments and in presence of, or absence of, light. In H₂O₂ rich environment in absence of light, variations decompose H₂O₂ into O₂ gas to remove excess H₂O₂ for elimination of hypoxic environment. Variations are formed of porphyrins, naphthalene derivatives, and metal ions, for illustration, free base tetrakis Ar substituted porphyrine core without metal or halide substitution but having hydroxyphenyl and alkyl pyridyl substituents at meso positions combined with dihydroxynaphthalene and +3 hydrated metal ions.

The invention belongs to the field of traditional Chinese medicine preparations, relates to a novel use of juglone having a structure shown in a formula (I) in pharmacy, and particularly relates to a use of the juglone in preparation of medicines treating brain tumor. In-vitro U87 spongioblastoma cell line killing tests and animal tests on a spongioblastoma model are performed. Results of the tests show that: the IC50 of the juglone in the killing tests is 6 [mu]g/mL; in the animal tests, the lifetime of a juglone group is longer than that of a contrast group; and by systematic antitumor medicine evaluation, the results prove that the juglone has significant inhibiting functions for the spongioblastoma in vitro and in vivo, and can be used for preparation of chemotherapeutics for treating the brain tumor especially the spongioblastoma. The formula (I) is shown in the specification.

The invention provides a method for separating and purifying juglone in walnut shells, wherein the method for separating and purifying juglone in the walnut shells comprises the following steps: S1, cleaning and drying the walnut shells, crushing the walnut shells by a crusher, and sieving the crushed walnut shells; S2, taking 20 g of walnut shell powder, placing in a steam explosion instrument, and pretreating; and S3, adding the walnut shell powder pretreated in the S2 into a solvent, and performing ultrasonic extraction at normal temperature, completely dissolving organic substances in the walnut shell powder into the solvent to obtain a mixed extracting solution. According to the method, the walnut shells are firstly crushed into the walnut shell powder, then purified crystals are obtained through the methods of steam explosion pretreatment, ultrasonic extraction and column chromatography purification, the process route is simple and feasible, juglone can be effectively extracted, separated and purified from the walnut shells, the product yield can reach 0.2% or above, waste utilization is facilitated, and the problem of ecological environment pollution caused by waste of a large number of walnut shells is avoided.

The invention relates to the technical field of medicines, discovers an application of juglone for adjusting immune states of mice and oxidizing reactions, and mainly researches medicinal composition juglone. A chemical name of the juglone is 5-hydroxy-1,4-naphthoquinone and 5-hydroxy-1,4-naphthalene diketone. An English name of the juglone is Juglone, walnuts quinine, 5-hydroxy-1,4-naphthoquinone and 5-hydroxynaphthalene, regianin, Nucin. A molecular formula of the juglone is C10H6O3. A relative molecular weight is 174.15. An immunocompromised model is built through systematic pharmacological tests and by using KM mice and adopting dexamethasone sodium phosphate injecta, a mouse immunostimulation model is built by using sheep red blood cells (SRBC) to induce the mice to produce SRBC immune bodies and cyclophosphamide, a fact that the juglone can recover low immunologic functions to a normal level is proved, the immunostimulation states are improved, self stability of an organism is maintained, and both-way immunoregulation functions are displayed. The juglone displays functions of adjusting organism immune system functions by adjusting oxidative stress reactions in the organism.

The invention discloses a hand and foot chapping nursing composition and a preparing method thereof .The hand and foot chapping nursing composition is mainly prepared from, by weight, 2-8 parts of sphingolipid, 1-5 parts of glycyrrhizin, 2-4 parts of asiaticoside, 3-6 parts of acetylsalicylic acid, 1-2 parts of theanine, 0.2-0.3 part of interleukin, 1-2 parts of oleanolic acid, 3-8 parts of sugar alcohol, 0.5-1.5 parts of beta-oxidized juglone, 3-6 parts of plant essential oil, 1-4 parts of water soluble vitamin and 3-6 parts of alginic acid .By combining the components in certain proportion, the functions of antisepsis and anti-inflammation, anti-infection, relieving swelling, pains and itching, removing necrosis, promoting granulation, promoting blood circulation, avoiding cicatrization and beautifying and nursing skin are realized, and then quick healing of chapped wounds is accelerated .The composite has a remarkable clinical treatment effect.

The invention discloses an application of juglone to preparation of a medicament for treating autoimmune and inflammatory diseases. As proved by an experimental result, the juglone can be used for lowering the disease incidence, average clinical score, highest clinical score and total clinical scores of mouse experimental autoimmune encephalomyelitis, lowering the percentages of an IFN (Interferon)-gamma secretion-relevant cell Th1 and a 1L-17A secretion-relevant cell Th17 in spinal marrow of mouse experimental autoimmune encephalomyelitis in a CD4+T cell, inhibiting the proliferation of a cell T induced by MOG (Myelin Oligodendrocyte Glycoprotein) of the mouse experimental autoimmune encephalomyelitis, and lowering the expression of CD11b+ angtigen presentation cells CD83 and MHCII in the spleen of the mouse experimental autoimmune encephalomyelitis. The juglone for preparing a medicament for inhibiting an immune system related to the invention is expected to become a medicament for clinically treating autoimmune inflammatory diseases such as multiple sclerosis, acute disseminated encephalomyelitis and the like.